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The map label for this gene is fruB [H]

Identifier: 29143513

GI number: 29143513

Start: 3268478

End: 3269284

Strand: Direct

Name: fruB [H]

Synonym: t3176

Alternate gene names: 29143513

Gene position: 3268478-3269284 (Clockwise)

Preceding gene: 29143512

Following gene: 29143514

Centisome position: 68.21

GC content: 53.28

Gene sequence:

>807_bases
ATGCAGCTCTGTGAACACGATATTTTTATCAGCGATGAGCGCCTCGATAAAGTCACCGCTCTGCACCGCGTGGTGGAAAA
GTTATCTGCGGCTGGCAATACCACGCCTGACTATTTACGCGGCATGTTGGATCGCGAAGCACAGATTTCCACTTATCTGG
GAAATGGAATCGCGATCCCACACGGTACGCCAGAATCTCGCGATGCGGTATTACAAACCGGTGTAAAGGTAATCGTTTTT
CGCCACGGCGTTGACTGGGGTGACGGCAATACCGCGTATCTGGTCACCGGAATTGCCGCGCGCTCCAATGAACATCTTGA
GATTTTGCGCCAACTGACTCGCGTGCTCAGCGATGACGCTATTCTGCAAGCACTGGCTAAAGCCGAATCCCCCAGTCAGG
TTTTAGCGCTACTGACTGGCTCGACGACCAATACGCCAGCAGCCATGGAGTTGCACGAGGGTGAGCAGGCAACATTTGTC
ATCCATAATCCGCATGGTTTACATGCCAGACCCAGTGCGGTACTGGTGAAGTTTATCAAACAGTTTCAAAGCCACATTAC
GGTAGAAAATCTGGATAACGCCTCCGGCCCGGTGGACGGAAAAAACCTGATGCGAGTGGTGTCGCTCGGCGCGAAAAAGG
GGCATCGCTTGTTGTTCCGGGCGCAGGGCGAAGATGCACAGCAGGCACTACGTGAAATCGGGGAGCTTATCGCCTCTGGT
GCAGGCGAGATGATCACCGTTCCCGTTACCCCACCACCAGAAGTCATGCAGCCAAAGCGCAGTTGGCTATCTCGATTGTT
TAATTAG

Upstream 100 bases:

>100_bases
GGATCTGCATGTGGTGCGATGATGCTTGGCCTATGGAGATTGTGGGCTGTGCGCAAAAATTCAGTCAACACAACTCCTGT
AGCAAAGGCTGGAGGTCAGA

Downstream 100 bases:

>100_bases
CCTTCATTTGTTCCTCCGTGCCCGCTCCGGCGGGTACGGTTTTTCATGACAAAGTGAAGTTACAGACATTACACACCATT
TCGTTACCTTACATTTTCTT

Product: phosphoryl transfer protein of PTS system

Products: protein histidine; sugar phosphate

Alternate protein names: MTP; Fructose PTS diphosphoryl transfer protein; Phosphotransferase FPr protein; Pseudo-HPr; Phosphocarrier protein HPr; Protein H; Fructose-specific phosphotransferase enzyme IIA component; EIIA-Fru; EIII-Fru; PTS system fructose-specific EIIA component [H]

Number of amino acids: Translated: 268; Mature: 268

Protein sequence:

>268_residues
MQLCEHDIFISDERLDKVTALHRVVEKLSAAGNTTPDYLRGMLDREAQISTYLGNGIAIPHGTPESRDAVLQTGVKVIVF
RHGVDWGDGNTAYLVTGIAARSNEHLEILRQLTRVLSDDAILQALAKAESPSQVLALLTGSTTNTPAAMELHEGEQATFV
IHNPHGLHARPSAVLVKFIKQFQSHITVENLDNASGPVDGKNLMRVVSLGAKKGHRLLFRAQGEDAQQALREIGELIASG
AGEMITVPVTPPPEVMQPKRSWLSRLFN

Sequences:

>Translated_268_residues
MQLCEHDIFISDERLDKVTALHRVVEKLSAAGNTTPDYLRGMLDREAQISTYLGNGIAIPHGTPESRDAVLQTGVKVIVF
RHGVDWGDGNTAYLVTGIAARSNEHLEILRQLTRVLSDDAILQALAKAESPSQVLALLTGSTTNTPAAMELHEGEQATFV
IHNPHGLHARPSAVLVKFIKQFQSHITVENLDNASGPVDGKNLMRVVSLGAKKGHRLLFRAQGEDAQQALREIGELIASG
AGEMITVPVTPPPEVMQPKRSWLSRLFN
>Mature_268_residues
MQLCEHDIFISDERLDKVTALHRVVEKLSAAGNTTPDYLRGMLDREAQISTYLGNGIAIPHGTPESRDAVLQTGVKVIVF
RHGVDWGDGNTAYLVTGIAARSNEHLEILRQLTRVLSDDAILQALAKAESPSQVLALLTGSTTNTPAAMELHEGEQATFV
IHNPHGLHARPSAVLVKFIKQFQSHITVENLDNASGPVDGKNLMRVVSLGAKKGHRLLFRAQGEDAQQALREIGELIASG
AGEMITVPVTPPPEVMQPKRSWLSRLFN

Specific function: The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. This

COG id: COG4668

COG function: function code G; Mannitol/fructose-specific phosphotransferase system, IIA domain

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PTS EIIA type-2 domain [H]

Homologues:

Organism=Escherichia coli, GI1788494, Length=140, Percent_Identity=52.1428571428571, Blast_Score=143, Evalue=1e-35,
Organism=Escherichia coli, GI1790027, Length=142, Percent_Identity=40.8450704225352, Blast_Score=108, Evalue=3e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016258
- InterPro:   IPR016152
- InterPro:   IPR002178
- InterPro:   IPR001020
- InterPro:   IPR005698
- InterPro:   IPR000032
- InterPro:   IPR002114 [H]

Pfam domain/function: PF00381 PTS-HPr; PF00359 PTS_EIIA_2 [H]

EC number: 2.7.1.69

Molecular weight: Translated: 29183; Mature: 29183

Theoretical pI: Translated: 6.75; Mature: 6.75

Prosite motif: PS00369 PTS_HPR_HIS ; PS00372 PTS_EIIA_TYPE_2_HIS ; PS51094 PTS_EIIA_TYPE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQLCEHDIFISDERLDKVTALHRVVEKLSAAGNTTPDYLRGMLDREAQISTYLGNGIAIP
CCCCCCCCEECHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCHHHHHHHHCCCEECC
HGTPESRDAVLQTGVKVIVFRHGVDWGDGNTAYLVTGIAARSNEHLEILRQLTRVLSDDA
CCCCCHHHHHHHCCCEEEEEECCCCCCCCCEEEEEEEEHHCCCHHHHHHHHHHHHHCHHH
ILQALAKAESPSQVLALLTGSTTNTPAAMELHEGEQATFVIHNPHGLHARPSAVLVKFIK
HHHHHHHCCCHHHEEHEEECCCCCCCCEEEEECCCCEEEEEECCCCCCCCHHHHHHHHHH
QFQSHITVENLDNASGPVDGKNLMRVVSLGAKKGHRLLFRAQGEDAQQALREIGELIASG
HHHHCCCHHCCCCCCCCCCHHHHHHHHHHCCCCCCEEEEEECCCHHHHHHHHHHHHHHCC
AGEMITVPVTPPPEVMQPKRSWLSRLFN
CCCEEEEECCCCHHHCCHHHHHHHHHCC
>Mature Secondary Structure
MQLCEHDIFISDERLDKVTALHRVVEKLSAAGNTTPDYLRGMLDREAQISTYLGNGIAIP
CCCCCCCCEECHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCHHHHHHHHCCCEECC
HGTPESRDAVLQTGVKVIVFRHGVDWGDGNTAYLVTGIAARSNEHLEILRQLTRVLSDDA
CCCCCHHHHHHHCCCEEEEEECCCCCCCCCEEEEEEEEHHCCCHHHHHHHHHHHHHCHHH
ILQALAKAESPSQVLALLTGSTTNTPAAMELHEGEQATFVIHNPHGLHARPSAVLVKFIK
HHHHHHHCCCHHHEEHEEECCCCCCCCEEEEECCCCEEEEEECCCCCCCCHHHHHHHHHH
QFQSHITVENLDNASGPVDGKNLMRVVSLGAKKGHRLLFRAQGEDAQQALREIGELIASG
HHHHCCCHHCCCCCCCCCCHHHHHHHHHHCCCCCCEEEEEECCCHHHHHHHHHHHHHHCC
AGEMITVPVTPPPEVMQPKRSWLSRLFN
CCCEEEEECCCCHHHCCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: protein N(pi)-phosphohistidine; sugar

Specific reaction: protein N(pi)-phosphohistidine + sugar = protein histidine + sugar phosphate

General reaction: Transferring phosphorus-containing groups [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800; 8763608 [H]