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The map label for this gene is uxuB [H]

Identifier: 29143401

GI number: 29143401

Start: 3138812

End: 3140284

Strand: Direct

Name: uxuB [H]

Synonym: t3057

Alternate gene names: 29143401

Gene position: 3138812-3140284 (Clockwise)

Preceding gene: 29143400

Following gene: 29143402

Centisome position: 65.5

GC content: 56.42

Gene sequence:

>1473_bases
ATGGAACAGAATATCGCCACCGCCCAGGTTTCCGTCGCCCGCCCAAACTGGGACAAATCACGTCTGGTATCCCGTATTGT
GCATCTGGGCTGCGGGGCGTTTCACCGCGCGCACCAGGCGCTCTTTACCCATCATCTACTGGAAAAGAGCGACAGCGACT
GGGGCATTTGTGAAGTGAACCTGATGCCGGGTAACGACGCGCGGCTGATCGCGAACCTGAAAGCGCAAAATCTGCTGTAC
ACCGTTGCAGAACGCGGCGCAGAAAGCACCGAACTGAAAATTATCGGTTCAATGAAAGAAGCGCTACACCCTGAATTCGA
CGGTCATGCAGGGATTCTGGCGGCAATGGCGCGCCCGGAAACCGCCATCGTCTCCTTAACCGTAACCGAAAAAGGCTACT
GCACCGACCCCGCCAGCGGCGAGCTTGATGTCAATAACCCGCTGATCCAAAACGATCTTGCCCATCCACAGCAGCCTAAA
TCCGCCATTGGCTATATTGTCGAAGCGCTAAACATGCGCCGGGAGCAAGGACTGAAAGCTTTTACCGTGCTGTCGTGCGA
TAACGTGCGCGAGAATGGTCACGTCGCGAAGGCTGCCGTCCTCGGCCTGGCAAAAGCTCGCGATGCCGCCCTCGCCGCAT
GGATTGCCGACAATGTGACCTTCCCCTGCACCATGGTTGACCGCATTGTTCCGGCAGCGACCGAAGAGACGCTACAACTG
GTTGCCGACCAGTTGGGCGTTTATGATCCTTGCGCTATCGCCTGCGAACCCTTCCGCCAGTGGGTCATCGAAGATAACTT
TGTGAATGGTCGCCCGGACTGGGATACCGTCGGCGCGCAATTCGTTGCAGATGTCGTACCGTTTGAGATGATGAAGCTGC
GTATGCTTAACGGCAGTCACTCTTTCCTCGCCTATCTGGGTTATCTCGGCGGGTATGACACTATCGCCGATACTATGACT
AACCCGGCTTACCGTCGCGCGGCGCTGGCGCTGATGCTTGATGAACAAGCGCCGACGTTGTCAATGCCGGAAGGCACCGA
TCTGGAAGGGTATGCGAATTTGTTGATTGCGCGTTTCACTAACCCCTCGTTGAAACACCGCACCTGGCAGATCGCGATGG
ATGGCAGTCAGAAACTGCCGCAGCGTCTGTTGGACCCGGTACGTCTGCACTTGCAACAGGGCGATGACTATCGCCGCCTG
ACGCTGGGCGTCGCCGGATGGATGCGTTATGTCGGCGGTGTCGATGAGCAAGGTAAAACCATTGATGTCGTCGATCCGCT
GCTTGCACAGTATCAGGCGATTCATCAGCAATATCAGACGCCGGAAGAACGCGTTCGCGGGCTACTGGCCATCGAGTCTA
TCTTTGGCAACGACCTGCCGAAGAACCACGAATTTGTGCAAGCCGTTACCGACGCTTACCAACAGCTATTGCAGAATGGC
GCGAAAGCCACGGTAGAAGCGCTGGCTAAGTAA

Upstream 100 bases:

>100_bases
CATGGCGCGACGCGTCATGCGATTTCCCCTACTCAATGCAATAGCAACATGCCTCGCCCCGGAGATCGCGGGCGAAGACG
TCGAATGACAGGAGTTTGCA

Downstream 100 bases:

>100_bases
GGAGATAAATCATGGCTACGTTTATGACTGAAGATTTTCTACTTAAAAACGACATTGCCCGCACGCTGTACCATAAATAC
GCCGCGCCCATGCCGATTTA

Product: D-mannonate oxidoreductase

Products: NA

Alternate protein names: Fructuronate reductase [H]

Number of amino acids: Translated: 490; Mature: 490

Protein sequence:

>490_residues
MEQNIATAQVSVARPNWDKSRLVSRIVHLGCGAFHRAHQALFTHHLLEKSDSDWGICEVNLMPGNDARLIANLKAQNLLY
TVAERGAESTELKIIGSMKEALHPEFDGHAGILAAMARPETAIVSLTVTEKGYCTDPASGELDVNNPLIQNDLAHPQQPK
SAIGYIVEALNMRREQGLKAFTVLSCDNVRENGHVAKAAVLGLAKARDAALAAWIADNVTFPCTMVDRIVPAATEETLQL
VADQLGVYDPCAIACEPFRQWVIEDNFVNGRPDWDTVGAQFVADVVPFEMMKLRMLNGSHSFLAYLGYLGGYDTIADTMT
NPAYRRAALALMLDEQAPTLSMPEGTDLEGYANLLIARFTNPSLKHRTWQIAMDGSQKLPQRLLDPVRLHLQQGDDYRRL
TLGVAGWMRYVGGVDEQGKTIDVVDPLLAQYQAIHQQYQTPEERVRGLLAIESIFGNDLPKNHEFVQAVTDAYQQLLQNG
AKATVEALAK

Sequences:

>Translated_490_residues
MEQNIATAQVSVARPNWDKSRLVSRIVHLGCGAFHRAHQALFTHHLLEKSDSDWGICEVNLMPGNDARLIANLKAQNLLY
TVAERGAESTELKIIGSMKEALHPEFDGHAGILAAMARPETAIVSLTVTEKGYCTDPASGELDVNNPLIQNDLAHPQQPK
SAIGYIVEALNMRREQGLKAFTVLSCDNVRENGHVAKAAVLGLAKARDAALAAWIADNVTFPCTMVDRIVPAATEETLQL
VADQLGVYDPCAIACEPFRQWVIEDNFVNGRPDWDTVGAQFVADVVPFEMMKLRMLNGSHSFLAYLGYLGGYDTIADTMT
NPAYRRAALALMLDEQAPTLSMPEGTDLEGYANLLIARFTNPSLKHRTWQIAMDGSQKLPQRLLDPVRLHLQQGDDYRRL
TLGVAGWMRYVGGVDEQGKTIDVVDPLLAQYQAIHQQYQTPEERVRGLLAIESIFGNDLPKNHEFVQAVTDAYQQLLQNG
AKATVEALAK
>Mature_490_residues
MEQNIATAQVSVARPNWDKSRLVSRIVHLGCGAFHRAHQALFTHHLLEKSDSDWGICEVNLMPGNDARLIANLKAQNLLY
TVAERGAESTELKIIGSMKEALHPEFDGHAGILAAMARPETAIVSLTVTEKGYCTDPASGELDVNNPLIQNDLAHPQQPK
SAIGYIVEALNMRREQGLKAFTVLSCDNVRENGHVAKAAVLGLAKARDAALAAWIADNVTFPCTMVDRIVPAATEETLQL
VADQLGVYDPCAIACEPFRQWVIEDNFVNGRPDWDTVGAQFVADVVPFEMMKLRMLNGSHSFLAYLGYLGGYDTIADTMT
NPAYRRAALALMLDEQAPTLSMPEGTDLEGYANLLIARFTNPSLKHRTWQIAMDGSQKLPQRLLDPVRLHLQQGDDYRRL
TLGVAGWMRYVGGVDEQGKTIDVVDPLLAQYQAIHQQYQTPEERVRGLLAIESIFGNDLPKNHEFVQAVTDAYQQLLQNG
AKATVEALAK

Specific function: Hexuronate conversion to KDG. [C]

COG id: COG0246

COG function: function code G; Mannitol-1-phosphate/altronate dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mannitol dehydrogenase family. UxuB subfamily [H]

Homologues:

Organism=Escherichia coli, GI1790779, Length=485, Percent_Identity=79.3814432989691, Blast_Score=807, Evalue=0.0,
Organism=Escherichia coli, GI1788497, Length=470, Percent_Identity=57.2340425531915, Blast_Score=553, Evalue=1e-158,
Organism=Escherichia coli, GI1787823, Length=485, Percent_Identity=57.1134020618557, Blast_Score=552, Evalue=1e-158,
Organism=Escherichia coli, GI48994885, Length=498, Percent_Identity=24.4979919678715, Blast_Score=110, Evalue=2e-25,
Organism=Escherichia coli, GI1790028, Length=256, Percent_Identity=27.734375, Blast_Score=89, Evalue=7e-19,
Organism=Saccharomyces cerevisiae, GI6324401, Length=490, Percent_Identity=39.5918367346939, Blast_Score=368, Evalue=1e-103,
Organism=Saccharomyces cerevisiae, GI6320765, Length=490, Percent_Identity=39.5918367346939, Blast_Score=368, Evalue=1e-103,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008927
- InterPro:   IPR013328
- InterPro:   IPR000669
- InterPro:   IPR013118
- InterPro:   IPR023027
- InterPro:   IPR013131
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01232 Mannitol_dh; PF08125 Mannitol_dh_C [H]

EC number: =1.1.1.57 [H]

Molecular weight: Translated: 54008; Mature: 54008

Theoretical pI: Translated: 5.30; Mature: 5.30

Prosite motif: PS00974 MANNITOL_DHGENASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEQNIATAQVSVARPNWDKSRLVSRIVHLGCGAFHRAHQALFTHHLLEKSDSDWGICEVN
CCCCCCEEEEEEECCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCCCEEEEE
LMPGNDARLIANLKAQNLLYTVAERGAESTELKIIGSMKEALHPEFDGHAGILAAMARPE
EECCCCCEEEEECHHHHHHHHHHHCCCCCCEEEEEEHHHHHHCCCCCCCHHHHHHHCCCC
TAIVSLTVTEKGYCTDPASGELDVNNPLIQNDLAHPQQPKSAIGYIVEALNMRREQGLKA
EEEEEEEEECCCCCCCCCCCCEECCCCCHHCCCCCCCCHHHHHHHHHHHHHHHHHCCCCE
FTVLSCDNVRENGHVAKAAVLGLAKARDAALAAWIADNVTFPCTMVDRIVPAATEETLQL
EEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHCCCCHHHHHHH
VADQLGVYDPCAIACEPFRQWVIEDNFVNGRPDWDTVGAQFVADVVPFEMMKLRMLNGSH
HHHHHCCCCCHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCH
SFLAYLGYLGGYDTIADTMTNPAYRRAALALMLDEQAPTLSMPEGTDLEGYANLLIARFT
HHHHHHHHHCCHHHHHHHHCCHHHHHHHHHHEECCCCCCCCCCCCCCCHHHHHEEEEECC
NPSLKHRTWQIAMDGSQKLPQRLLDPVRLHLQQGDDYRRLTLGVAGWMRYVGGVDEQGKT
CCCCCCEEEEEEECCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCCE
IDVVDPLLAQYQAIHQQYQTPEERVRGLLAIESIFGNDLPKNHEFVQAVTDAYQQLLQNG
EHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCC
AKATVEALAK
HHHHHHHHCC
>Mature Secondary Structure
MEQNIATAQVSVARPNWDKSRLVSRIVHLGCGAFHRAHQALFTHHLLEKSDSDWGICEVN
CCCCCCEEEEEEECCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCCCEEEEE
LMPGNDARLIANLKAQNLLYTVAERGAESTELKIIGSMKEALHPEFDGHAGILAAMARPE
EECCCCCEEEEECHHHHHHHHHHHCCCCCCEEEEEEHHHHHHCCCCCCCHHHHHHHCCCC
TAIVSLTVTEKGYCTDPASGELDVNNPLIQNDLAHPQQPKSAIGYIVEALNMRREQGLKA
EEEEEEEEECCCCCCCCCCCCEECCCCCHHCCCCCCCCHHHHHHHHHHHHHHHHHCCCCE
FTVLSCDNVRENGHVAKAAVLGLAKARDAALAAWIADNVTFPCTMVDRIVPAATEETLQL
EEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHCCCCHHHHHHH
VADQLGVYDPCAIACEPFRQWVIEDNFVNGRPDWDTVGAQFVADVVPFEMMKLRMLNGSH
HHHHHCCCCCHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCH
SFLAYLGYLGGYDTIADTMTNPAYRRAALALMLDEQAPTLSMPEGTDLEGYANLLIARFT
HHHHHHHHHCCHHHHHHHHCCHHHHHHHHHHEECCCCCCCCCCCCCCCHHHHHEEEEECC
NPSLKHRTWQIAMDGSQKLPQRLLDPVRLHLQQGDDYRRLTLGVAGWMRYVGGVDEQGKT
CCCCCCEEEEEEECCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCCE
IDVVDPLLAQYQAIHQQYQTPEERVRGLLAIESIFGNDLPKNHEFVQAVTDAYQQLLQNG
EHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCC
AKATVEALAK
HHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7610040; 9278503 [H]