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The map label for this gene is csdA [H]

Identifier: 29143246

GI number: 29143246

Start: 2977389

End: 2978594

Strand: Direct

Name: csdA [H]

Synonym: t2892

Alternate gene names: 29143246

Gene position: 2977389-2978594 (Clockwise)

Preceding gene: 29143241

Following gene: 29143247

Centisome position: 62.13

GC content: 59.54

Gene sequence:

>1206_bases
ATGAACGCTTTTAATCCCACGCAGTTTCGCGCGCAGTTTCCCGCGCTAGCCGATGCGGGTGTTTATCTCGATAGCGCCGC
CACGGCATTAAAGCCACAGGCAGTCATTGACGCCACGCACCAGTTTTATTGTTTGAGCGCCGGTAACGTTCATCGTAGCC
AGTTTGCGCAGGCGCAGCGCCTGACGGCGCAATATGAAGCGGCCAGAGCAAAAGCGGCGCGGCTGTTAAACGCGCCCGAT
GAAAAAAGTATCGTCTGGACACGCGGCACCACCGAAGCGATCAACATGGTGGCGCAGTGTTACGCCCGTCCTCGTCTGCG
CCCCGGCGATGAAATTATCGTTAGCGTCGCCGAGCATCACGCCAACCTTGTTCCCTGGCTGATGGTGGCGCAACAAACCG
GCGCGCAGGTCATAAAACTGCCGCTTAATGACCGACGTCTTCCTGATGTTGAGCGTCTGCCGGAACTGATCACGTCGCGC
AGCCGGATACTGGCGCTGGGGCAAATGTCGAACGTAACGGGCGGCTGTCCGGATCTCACAGGCGCTATCAGCGCCGCTCA
CGCGGCGGGAATGGTCGTGATGATAGATGGCGCGCAAGGCGCTGTACACTTCCCGGCGGATGTCCAGCAGCTTGATATCG
ATTTTTATGCTTTTTCCGCTCACAAACTGTATGGCCCGACCGGTATCGGCGTGCTGTACGGTAAGCCGGAGCTTCTTGAG
GCGATGTCGCCCTGGCTCGGCGGCGGCAAGATGATCCGTGACGTTAGCTTTGAAGGCTTCACCACTCAAAGCGCTCCCTG
GAAACTGGAAGCGGGGACGCCGAACGTCGCCGGGGTCATCGGCCTGAGCGCTGCGCTGGAATGGCTGTCCGATATCGATA
TTGAACAGGCCGAAAACTGGAGCCGGGGGCTGGCGACGCTGGCGGAAGACGCACTGGCGAAACGTCCGGGCTTTCGTTCG
TTCCGCTGCCAGGACTCCAGCCTGCTGGCCTTTGATTTTGTCGGCGTGCACCACGGCGATATGGTGACGCTGCTGGCGGA
ATACGGTATTGCGCTCCGGGCCGGGCAACATTGCGCCCAGCCATTGCTAGCGGAACTTGGCGTCACAGGGACTCTGCGCG
CCTCTTTTGCGCCGTATAATACCCAACATGATGTGGATGCGTTGGTTAACGCCGTTGACCGCGCGCTGGAACTGCTGGTG
GATTAA

Upstream 100 bases:

>100_bases
CATAATTCGCCAGGATATACAGACGATTCCGATGGTTAGGACAAACCTTGTATCAGCCGCGCTTTTACGCCACGATCGGT
AAACTGAACCGAGGAATATC

Downstream 100 bases:

>100_bases
TGACAAACCCTCTTTATACCGGACACCCGTTCGGCACAACGGTAACGGAAGAGACGTTACGCGCTATCTTTCTCCCGCTG
ACCCAATGGGAGGATAAATA

Product: cysteine sulfinate desulfinase

Products: @ALAN01txt*L-Alanine! [C]

Alternate protein names: CSD [H]

Number of amino acids: Translated: 401; Mature: 401

Protein sequence:

>401_residues
MNAFNPTQFRAQFPALADAGVYLDSAATALKPQAVIDATHQFYCLSAGNVHRSQFAQAQRLTAQYEAARAKAARLLNAPD
EKSIVWTRGTTEAINMVAQCYARPRLRPGDEIIVSVAEHHANLVPWLMVAQQTGAQVIKLPLNDRRLPDVERLPELITSR
SRILALGQMSNVTGGCPDLTGAISAAHAAGMVVMIDGAQGAVHFPADVQQLDIDFYAFSAHKLYGPTGIGVLYGKPELLE
AMSPWLGGGKMIRDVSFEGFTTQSAPWKLEAGTPNVAGVIGLSAALEWLSDIDIEQAENWSRGLATLAEDALAKRPGFRS
FRCQDSSLLAFDFVGVHHGDMVTLLAEYGIALRAGQHCAQPLLAELGVTGTLRASFAPYNTQHDVDALVNAVDRALELLV
D

Sequences:

>Translated_401_residues
MNAFNPTQFRAQFPALADAGVYLDSAATALKPQAVIDATHQFYCLSAGNVHRSQFAQAQRLTAQYEAARAKAARLLNAPD
EKSIVWTRGTTEAINMVAQCYARPRLRPGDEIIVSVAEHHANLVPWLMVAQQTGAQVIKLPLNDRRLPDVERLPELITSR
SRILALGQMSNVTGGCPDLTGAISAAHAAGMVVMIDGAQGAVHFPADVQQLDIDFYAFSAHKLYGPTGIGVLYGKPELLE
AMSPWLGGGKMIRDVSFEGFTTQSAPWKLEAGTPNVAGVIGLSAALEWLSDIDIEQAENWSRGLATLAEDALAKRPGFRS
FRCQDSSLLAFDFVGVHHGDMVTLLAEYGIALRAGQHCAQPLLAELGVTGTLRASFAPYNTQHDVDALVNAVDRALELLV
D
>Mature_401_residues
MNAFNPTQFRAQFPALADAGVYLDSAATALKPQAVIDATHQFYCLSAGNVHRSQFAQAQRLTAQYEAARAKAARLLNAPD
EKSIVWTRGTTEAINMVAQCYARPRLRPGDEIIVSVAEHHANLVPWLMVAQQTGAQVIKLPLNDRRLPDVERLPELITSR
SRILALGQMSNVTGGCPDLTGAISAAHAAGMVVMIDGAQGAVHFPADVQQLDIDFYAFSAHKLYGPTGIGVLYGKPELLE
AMSPWLGGGKMIRDVSFEGFTTQSAPWKLEAGTPNVAGVIGLSAALEWLSDIDIEQAENWSRGLATLAEDALAKRPGFRS
FRCQDSSLLAFDFVGVHHGDMVTLLAEYGIALRAGQHCAQPLLAELGVTGTLRASFAPYNTQHDVDALVNAVDRALELLV
D

Specific function: Catalyzes the removal of elemental sulfur and selenium atoms from L-cysteine, L-cystine, L-selenocysteine, and L- selenocystine to produce L-alanine. L-cysteine sulfinic acid is the best substrate. Functions as a selenium delivery protein in the pathway f

COG id: COG0520

COG function: function code E; Selenocysteine lyase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-V pyridoxal-phosphate-dependent aminotransferase family. Csd subfamily [H]

Homologues:

Organism=Homo sapiens, GI32307132, Length=271, Percent_Identity=26.9372693726937, Blast_Score=79, Evalue=7e-15,
Organism=Escherichia coli, GI1789175, Length=401, Percent_Identity=88.7780548628429, Blast_Score=743, Evalue=0.0,
Organism=Escherichia coli, GI1787970, Length=404, Percent_Identity=44.0594059405941, Blast_Score=324, Evalue=7e-90,
Organism=Escherichia coli, GI48994898, Length=215, Percent_Identity=28.3720930232558, Blast_Score=85, Evalue=8e-18,
Organism=Caenorhabditis elegans, GI25143064, Length=213, Percent_Identity=28.6384976525822, Blast_Score=98, Evalue=7e-21,
Organism=Caenorhabditis elegans, GI193211090, Length=353, Percent_Identity=25.7790368271955, Blast_Score=75, Evalue=5e-14,
Organism=Saccharomyces cerevisiae, GI6319831, Length=401, Percent_Identity=27.431421446384, Blast_Score=107, Evalue=5e-24,
Organism=Drosophila melanogaster, GI20129463, Length=280, Percent_Identity=27.5, Blast_Score=92, Evalue=8e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000192
- InterPro:   IPR020578
- InterPro:   IPR022471
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF00266 Aminotran_5 [H]

EC number: 4.4.1.- [C]

Molecular weight: Translated: 43184; Mature: 43184

Theoretical pI: Translated: 5.76; Mature: 5.76

Prosite motif: PS00595 AA_TRANSFER_CLASS_5

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNAFNPTQFRAQFPALADAGVYLDSAATALKPQAVIDATHQFYCLSAGNVHRSQFAQAQR
CCCCCCCHHHHHCCCHHCCCEEECCHHHHCCCHHHHCCCCEEEEEECCCCCHHHHHHHHH
LTAQYEAARAKAARLLNAPDEKSIVWTRGTTEAINMVAQCYARPRLRPGDEIIVSVAEHH
HHHHHHHHHHHHHHHHCCCCCCCEEEECCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHC
ANLVPWLMVAQQTGAQVIKLPLNDRRLPDVERLPELITSRSRILALGQMSNVTGGCPDLT
CCHHHHHHHHHHCCCEEEEECCCCCCCCCHHHHHHHHCCCHHEEEEECCCCCCCCCCCHH
GAISAAHAAGMVVMIDGAQGAVHFPADVQQLDIDFYAFSAHKLYGPTGIGVLYGKPELLE
HHHHHHHHCCEEEEEECCCCCEECCCCCCCEEEEEEEEECCEEECCCCCEEEECCHHHHH
AMSPWLGGGKMIRDVSFEGFTTQSAPWKLEAGTPNVAGVIGLSAALEWLSDIDIEQAENW
HHCCCCCCCCEEEEECCCCCCCCCCCEEEECCCCCHHHHHHHHHHHHHHHHCCHHHHHHH
SRGLATLAEDALAKRPGFRSFRCQDSSLLAFDFVGVHHGDMVTLLAEYGIALRAGQHCAQ
HHHHHHHHHHHHHHCCCCCCEECCCCCEEEEEEEECCCCHHHHHHHHCCHHHHCCHHHHH
PLLAELGVTGTLRASFAPYNTQHDVDALVNAVDRALELLVD
HHHHHHCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MNAFNPTQFRAQFPALADAGVYLDSAATALKPQAVIDATHQFYCLSAGNVHRSQFAQAQR
CCCCCCCHHHHHCCCHHCCCEEECCHHHHCCCHHHHCCCCEEEEEECCCCCHHHHHHHHH
LTAQYEAARAKAARLLNAPDEKSIVWTRGTTEAINMVAQCYARPRLRPGDEIIVSVAEHH
HHHHHHHHHHHHHHHHCCCCCCCEEEECCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHC
ANLVPWLMVAQQTGAQVIKLPLNDRRLPDVERLPELITSRSRILALGQMSNVTGGCPDLT
CCHHHHHHHHHHCCCEEEEECCCCCCCCCHHHHHHHHCCCHHEEEEECCCCCCCCCCCHH
GAISAAHAAGMVVMIDGAQGAVHFPADVQQLDIDFYAFSAHKLYGPTGIGVLYGKPELLE
HHHHHHHHCCEEEEEECCCCCEECCCCCCCEEEEEEEEECCEEECCCCCEEEECCHHHHH
AMSPWLGGGKMIRDVSFEGFTTQSAPWKLEAGTPNVAGVIGLSAALEWLSDIDIEQAENW
HHCCCCCCCCEEEEECCCCCCCCCCCEEEECCCCCHHHHHHHHHHHHHHHHCCHHHHHHH
SRGLATLAEDALAKRPGFRSFRCQDSSLLAFDFVGVHHGDMVTLLAEYGIALRAGQHCAQ
HHHHHHHHHHHHHHCCCCCCEECCCCCEEEEEEEECCCCHHHHHHHHCCHHHHCCHHHHH
PLLAELGVTGTLRASFAPYNTQHDVDALVNAVDRALELLVD
HHHHHHCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: Pyridoxal phosphate [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: L-cystine; L-selenocystine [C]

Specific reaction: Catalyzes the removal of elemental sulfur and selenium atoms from l-cysteine, l-cystine, l-selenocysteine, and l- selenocystine to produce @ALAN01.txt*l-Alanine! [C]

General reaction: Remove sulfur and selenium atoms [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9278392; 9278503; 10829016; 10739946 [H]