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The map label for this gene is cysN [H]

Identifier: 29143190

GI number: 29143190

Start: 2908372

End: 2909811

Strand: Reverse

Name: cysN [H]

Synonym: t2835

Alternate gene names: 29143190

Gene position: 2909811-2908372 (Counterclockwise)

Preceding gene: 29143191

Following gene: 29143189

Centisome position: 60.72

GC content: 54.79

Gene sequence:

>1440_bases
ATGAACACCATACTTGCACAACAAATCGCCAGTGAAGGCGGCGTCGAAGCCTGGATGATTGCCCAACAACACAAAAGCCT
GCTGCGTTTTTTAACCTGTGGCAGCGTTGATGACGGTAAAAGTACGCTGATAGGTCGTTTGCTGCACGATACCCTGCAAA
TTTATGAAGATCAGCTCTCTTCTCTGCATAATGACAGTAAACGTCATGGCACGCAGGGAGAAAAACTCGATCTGGCGCTG
CTGGTAGATGGGCTGCAGGCCGAGCGTGAGCAGGGCATTACTATTGACGTCGCGTACCGCTATTTCTCCACGGAAAAACG
AAAATTTATTATCGCCGACACGCCGGGCCATGAACAATATACCCGTAATATGGCGACCGGAGCGTCCACCTGCGATCTGG
CGATCCTACTGATCGACGCGCGTAAAGGCGTGCTGGATCAGACGCGTCGCCATAGCTTTATCTCCACTCTGTTGGGGATC
AAACACCTGGTGGTGGCAATCAACAAAATGGATCTCGTTGACTACCGCGAAGAGACCTTCGCCCGTATTCGCGAAGATTA
CCTGACCTTTGCTGAACAGTTGCCGGGCGATCTGGACATTCGCTTTGTCCCGCTTTCGGCGTTGGAAGGGGATAATGTCG
CCGCGCAGAGCGCCAATATGCGCTGGTACAGCGGCCCGACGCTGTTGGAAGTCCTGGAAACGGTAGACATACAGCGTGCG
GTAGACCGTCAGCCGATGCGCTTTCCAGTACAATACGTCAATCGCCCGAACCCTGATTTTCGGGGGTATGCCGGCACTCT
GGCGTCTGGTAGCGTCAAAGTCGGAGAGCGCATTAAAGTGCTGCCGTCCGGCGTGGAATCCAGCGTGGCGCGTATCGTCA
CTTTTGATGGCGACAAAGAAGAAGTCTGTGCGGGCGAAGCCATTACCCTGGTACTCAACGACGACATTGATATTAGCCGC
GGCGATCTGCTGCTGGCGGCAAACGAGACGCTGGCACCTGCGCGGCACGCCGCGATCGATGTGGTATGGATGGCGGAACA
GCCGCTGGCGCCGGGCCAAAGCTACGACGTTAAACTTGCGGGTAAGAAAACCCGCGCGCGTATCGAGGCTATTTGCTATC
AGATTGATATCAACAATCTGACTCAACGAGACGTCGAGAGCCTGCCGTTAAACGGTATTGGTCTGGTAGAGATGACATTC
GATGAGCCGCTCGCGCTGGATATTTATCAGCAAAATCCGGTGACGGGCGGGCTGATTTTTATCGACCGACTTTCTAACGT
TACCGTGGGTGCTGGCATGGTGCGGGAACTGGATGAACGCGGAGCGACGCCGCCCGTGGAATACAGTGCGTTTGAGCTGG
AGCTGAATGCGCTGGTACGCCGTCATTTTCCGCACTGGGACGCCCGAGATTTGCTGGGAGATAAACATGGCGCTGCATGA

Upstream 100 bases:

>100_bases
GGTTTCTACCACCAGCGAGCGCCAGGGGAGGATGATTGACCGCGATCAGGCGGGGTCGATGGAGCTTAAAAAACGTCAGG
GGTATTTCTAAGGAGCCGCC

Downstream 100 bases:

>100_bases
TGAGAACGTGGTCTGGCACTCTCATCCCGTTACTGTCGCCGCTCGTGAACAGCTCCACGGCCACCGTGGCGTGGTGCTGT
GGTTTACCGGACTCTCCGGT

Product: sulfate adenylyltransferase subunit 1

Products: NA

Alternate protein names: ATP-sulfurylase large subunit; Sulfate adenylate transferase; SAT [H]

Number of amino acids: Translated: 479; Mature: 479

Protein sequence:

>479_residues
MNTILAQQIASEGGVEAWMIAQQHKSLLRFLTCGSVDDGKSTLIGRLLHDTLQIYEDQLSSLHNDSKRHGTQGEKLDLAL
LVDGLQAEREQGITIDVAYRYFSTEKRKFIIADTPGHEQYTRNMATGASTCDLAILLIDARKGVLDQTRRHSFISTLLGI
KHLVVAINKMDLVDYREETFARIREDYLTFAEQLPGDLDIRFVPLSALEGDNVAAQSANMRWYSGPTLLEVLETVDIQRA
VDRQPMRFPVQYVNRPNPDFRGYAGTLASGSVKVGERIKVLPSGVESSVARIVTFDGDKEEVCAGEAITLVLNDDIDISR
GDLLLAANETLAPARHAAIDVVWMAEQPLAPGQSYDVKLAGKKTRARIEAICYQIDINNLTQRDVESLPLNGIGLVEMTF
DEPLALDIYQQNPVTGGLIFIDRLSNVTVGAGMVRELDERGATPPVEYSAFELELNALVRRHFPHWDARDLLGDKHGAA

Sequences:

>Translated_479_residues
MNTILAQQIASEGGVEAWMIAQQHKSLLRFLTCGSVDDGKSTLIGRLLHDTLQIYEDQLSSLHNDSKRHGTQGEKLDLAL
LVDGLQAEREQGITIDVAYRYFSTEKRKFIIADTPGHEQYTRNMATGASTCDLAILLIDARKGVLDQTRRHSFISTLLGI
KHLVVAINKMDLVDYREETFARIREDYLTFAEQLPGDLDIRFVPLSALEGDNVAAQSANMRWYSGPTLLEVLETVDIQRA
VDRQPMRFPVQYVNRPNPDFRGYAGTLASGSVKVGERIKVLPSGVESSVARIVTFDGDKEEVCAGEAITLVLNDDIDISR
GDLLLAANETLAPARHAAIDVVWMAEQPLAPGQSYDVKLAGKKTRARIEAICYQIDINNLTQRDVESLPLNGIGLVEMTF
DEPLALDIYQQNPVTGGLIFIDRLSNVTVGAGMVRELDERGATPPVEYSAFELELNALVRRHFPHWDARDLLGDKHGAA
>Mature_479_residues
MNTILAQQIASEGGVEAWMIAQQHKSLLRFLTCGSVDDGKSTLIGRLLHDTLQIYEDQLSSLHNDSKRHGTQGEKLDLAL
LVDGLQAEREQGITIDVAYRYFSTEKRKFIIADTPGHEQYTRNMATGASTCDLAILLIDARKGVLDQTRRHSFISTLLGI
KHLVVAINKMDLVDYREETFARIREDYLTFAEQLPGDLDIRFVPLSALEGDNVAAQSANMRWYSGPTLLEVLETVDIQRA
VDRQPMRFPVQYVNRPNPDFRGYAGTLASGSVKVGERIKVLPSGVESSVARIVTFDGDKEEVCAGEAITLVLNDDIDISR
GDLLLAANETLAPARHAAIDVVWMAEQPLAPGQSYDVKLAGKKTRARIEAICYQIDINNLTQRDVESLPLNGIGLVEMTF
DEPLALDIYQQNPVTGGLIFIDRLSNVTVGAGMVRELDERGATPPVEYSAFELELNALVRRHFPHWDARDLLGDKHGAA

Specific function: May be the GTPase, regulating ATP sulfurylase activity [H]

COG id: COG2895

COG function: function code P; GTPases - Sulfate adenylate transferase subunit 1

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP-binding elongation factor family. CysN/nodQ subfamily [H]

Homologues:

Organism=Homo sapiens, GI223555963, Length=455, Percent_Identity=29.8901098901099, Blast_Score=168, Evalue=1e-41,
Organism=Homo sapiens, GI5729864, Length=455, Percent_Identity=29.8901098901099, Blast_Score=167, Evalue=2e-41,
Organism=Homo sapiens, GI194018522, Length=334, Percent_Identity=32.0359281437126, Blast_Score=153, Evalue=3e-37,
Organism=Homo sapiens, GI194018520, Length=316, Percent_Identity=32.9113924050633, Blast_Score=153, Evalue=3e-37,
Organism=Homo sapiens, GI194097354, Length=316, Percent_Identity=32.9113924050633, Blast_Score=153, Evalue=4e-37,
Organism=Homo sapiens, GI46094014, Length=292, Percent_Identity=33.2191780821918, Blast_Score=150, Evalue=3e-36,
Organism=Homo sapiens, GI4503471, Length=450, Percent_Identity=26.8888888888889, Blast_Score=145, Evalue=6e-35,
Organism=Homo sapiens, GI4503475, Length=448, Percent_Identity=26.3392857142857, Blast_Score=144, Evalue=2e-34,
Organism=Homo sapiens, GI34147630, Length=341, Percent_Identity=27.2727272727273, Blast_Score=86, Evalue=8e-17,
Organism=Escherichia coli, GI1789108, Length=475, Percent_Identity=87.1578947368421, Blast_Score=855, Evalue=0.0,
Organism=Escherichia coli, GI1790412, Length=420, Percent_Identity=26.1904761904762, Blast_Score=86, Evalue=4e-18,
Organism=Escherichia coli, GI1789737, Length=420, Percent_Identity=26.1904761904762, Blast_Score=86, Evalue=4e-18,
Organism=Escherichia coli, GI2367247, Length=354, Percent_Identity=25.9887005649718, Blast_Score=72, Evalue=5e-14,
Organism=Caenorhabditis elegans, GI115532067, Length=427, Percent_Identity=25.9953161592506, Blast_Score=162, Evalue=3e-40,
Organism=Caenorhabditis elegans, GI115532065, Length=427, Percent_Identity=25.9953161592506, Blast_Score=162, Evalue=5e-40,
Organism=Caenorhabditis elegans, GI32566629, Length=427, Percent_Identity=27.8688524590164, Blast_Score=157, Evalue=1e-38,
Organism=Caenorhabditis elegans, GI17552884, Length=441, Percent_Identity=28.7981859410431, Blast_Score=149, Evalue=3e-36,
Organism=Caenorhabditis elegans, GI17569207, Length=441, Percent_Identity=28.7981859410431, Blast_Score=149, Evalue=3e-36,
Organism=Caenorhabditis elegans, GI32566303, Length=434, Percent_Identity=27.4193548387097, Blast_Score=121, Evalue=8e-28,
Organism=Caenorhabditis elegans, GI25141371, Length=229, Percent_Identity=27.0742358078603, Blast_Score=91, Evalue=9e-19,
Organism=Caenorhabditis elegans, GI32566301, Length=138, Percent_Identity=36.9565217391304, Blast_Score=87, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI17556456, Length=336, Percent_Identity=28.5714285714286, Blast_Score=87, Evalue=2e-17,
Organism=Saccharomyces cerevisiae, GI6322937, Length=446, Percent_Identity=28.0269058295964, Blast_Score=158, Evalue=2e-39,
Organism=Saccharomyces cerevisiae, GI6325337, Length=324, Percent_Identity=30.2469135802469, Blast_Score=148, Evalue=2e-36,
Organism=Saccharomyces cerevisiae, GI6319594, Length=324, Percent_Identity=30.2469135802469, Blast_Score=148, Evalue=2e-36,
Organism=Saccharomyces cerevisiae, GI6320377, Length=432, Percent_Identity=27.0833333333333, Blast_Score=122, Evalue=1e-28,
Organism=Saccharomyces cerevisiae, GI6324761, Length=145, Percent_Identity=35.1724137931034, Blast_Score=84, Evalue=7e-17,
Organism=Drosophila melanogaster, GI45550900, Length=433, Percent_Identity=28.175519630485, Blast_Score=155, Evalue=7e-38,
Organism=Drosophila melanogaster, GI24652838, Length=439, Percent_Identity=25.9681093394077, Blast_Score=137, Evalue=1e-32,
Organism=Drosophila melanogaster, GI17137572, Length=439, Percent_Identity=25.9681093394077, Blast_Score=137, Evalue=1e-32,
Organism=Drosophila melanogaster, GI45553807, Length=439, Percent_Identity=25.9681093394077, Blast_Score=136, Evalue=4e-32,
Organism=Drosophila melanogaster, GI45553816, Length=439, Percent_Identity=25.9681093394077, Blast_Score=136, Evalue=4e-32,
Organism=Drosophila melanogaster, GI24651721, Length=439, Percent_Identity=25.9681093394077, Blast_Score=136, Evalue=4e-32,
Organism=Drosophila melanogaster, GI17864154, Length=439, Percent_Identity=25.9681093394077, Blast_Score=136, Evalue=4e-32,
Organism=Drosophila melanogaster, GI17137380, Length=312, Percent_Identity=29.4871794871795, Blast_Score=131, Evalue=8e-31,
Organism=Drosophila melanogaster, GI281363316, Length=302, Percent_Identity=28.476821192053, Blast_Score=91, Evalue=3e-18,
Organism=Drosophila melanogaster, GI17864358, Length=302, Percent_Identity=28.476821192053, Blast_Score=91, Evalue=3e-18,
Organism=Drosophila melanogaster, GI19921738, Length=307, Percent_Identity=27.6872964169381, Blast_Score=81, Evalue=1e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000795
- InterPro:   IPR005225
- InterPro:   IPR011779
- InterPro:   IPR009001
- InterPro:   IPR004161
- InterPro:   IPR009000 [H]

Pfam domain/function: PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2 [H]

EC number: =2.7.7.4 [H]

Molecular weight: Translated: 53067; Mature: 53067

Theoretical pI: Translated: 4.81; Mature: 4.81

Prosite motif: PS00301 EFACTOR_GTP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNTILAQQIASEGGVEAWMIAQQHKSLLRFLTCGSVDDGKSTLIGRLLHDTLQIYEDQLS
CCCHHHHHHHHCCCCEEEHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHH
SLHNDSKRHGTQGEKLDLALLVDGLQAEREQGITIDVAYRYFSTEKRKFIIADTPGHEQY
HHHCCHHHCCCCCCCEEEEEEECCHHHHHHCCCEEEEEEEEECCCCCEEEEEECCCCHHH
TRNMATGASTCDLAILLIDARKGVLDQTRRHSFISTLLGIKHLVVAINKMDLVDYREETF
HHHHHCCCCCCEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ARIREDYLTFAEQLPGDLDIRFVPLSALEGDNVAAQSANMRWYSGPTLLEVLETVDIQRA
HHHHHHHHHHHHHCCCCCEEEEEEEECCCCCCCEECCCCCEEECCCHHHHHHHHHHHHHH
VDRQPMRFPVQYVNRPNPDFRGYAGTLASGSVKVGERIKVLPSGVESSVARIVTFDGDKE
HCCCCCCCCHHHCCCCCCCCCCCCCCCCCCCEECCCEEEECCCCCCCCEEEEEEECCCHH
EVCAGEAITLVLNDDIDISRGDLLLAANETLAPARHAAIDVVWMAEQPLAPGQSYDVKLA
HHCCCCEEEEEEECCCCCCCCCEEEEECCCCCCCCCCEEEEEEEECCCCCCCCCCEEEEC
GKKTRARIEAICYQIDINNLTQRDVESLPLNGIGLVEMTFDEPLALDIYQQNPVTGGLIF
CCHHHHEEEEEEEEEECCCCCHHHHHHCCCCCCEEEEEECCCCEEEEEEECCCCCCCEEE
IDRLSNVTVGAGMVRELDERGATPPVEYSAFELELNALVRRHFPHWDARDLLGDKHGAA
EECCCCCEECHHHHHHHHHCCCCCCCCCEEEEEEHHHHHHHCCCCCCHHHHCCCCCCCC
>Mature Secondary Structure
MNTILAQQIASEGGVEAWMIAQQHKSLLRFLTCGSVDDGKSTLIGRLLHDTLQIYEDQLS
CCCHHHHHHHHCCCCEEEHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHH
SLHNDSKRHGTQGEKLDLALLVDGLQAEREQGITIDVAYRYFSTEKRKFIIADTPGHEQY
HHHCCHHHCCCCCCCEEEEEEECCHHHHHHCCCEEEEEEEEECCCCCEEEEEECCCCHHH
TRNMATGASTCDLAILLIDARKGVLDQTRRHSFISTLLGIKHLVVAINKMDLVDYREETF
HHHHHCCCCCCEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ARIREDYLTFAEQLPGDLDIRFVPLSALEGDNVAAQSANMRWYSGPTLLEVLETVDIQRA
HHHHHHHHHHHHHCCCCCEEEEEEEECCCCCCCEECCCCCEEECCCHHHHHHHHHHHHHH
VDRQPMRFPVQYVNRPNPDFRGYAGTLASGSVKVGERIKVLPSGVESSVARIVTFDGDKE
HCCCCCCCCHHHCCCCCCCCCCCCCCCCCCCEECCCEEEECCCCCCCCEEEEEEECCCHH
EVCAGEAITLVLNDDIDISRGDLLLAANETLAPARHAAIDVVWMAEQPLAPGQSYDVKLA
HHCCCCEEEEEEECCCCCCCCCEEEEECCCCCCCCCCEEEEEEEECCCCCCCCCCEEEEC
GKKTRARIEAICYQIDINNLTQRDVESLPLNGIGLVEMTFDEPLALDIYQQNPVTGGLIF
CCHHHHEEEEEEEEEECCCCCHHHHHHCCCCCCEEEEEECCCCEEEEEEECCCCCCCEEE
IDRLSNVTVGAGMVRELDERGATPPVEYSAFELELNALVRRHFPHWDARDLLGDKHGAA
EECCCCCEECHHHHHHHHHCCCCCCCCCEEEEEEHHHHHHHCCCCCCHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA