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The map label for this gene is rpoS [H]

Identifier: 29143180

GI number: 29143180

Start: 2900774

End: 2901928

Strand: Reverse

Name: rpoS [H]

Synonym: t2825

Alternate gene names: 29143180

Gene position: 2901928-2900774 (Counterclockwise)

Preceding gene: 29143181

Following gene: 29143176

Centisome position: 60.56

GC content: 52.21

Gene sequence:

>1155_bases
ATGAGTCAGAATACGCTGAAAGTTCATGATTTAAATGAAGACGCGGAATTTGATGAGAACGGAGTAGAGGCTTTTGACGA
AAAAGCCTTGAGTGAAGAGGAACCCAGTGATAACGACCTGGCTGAAGAAGAGCTGTTATCGCAAGGGGCCACACAGCGTG
TGTTGGACGCGACTCAGCTTTACCTTGGTGAGATTGGGTATTCACCACTGTTAACAGCCGAAGAAGAAGTCTATTTTGCG
CGTCGCGCACTGCGTGGAGATGTCGCTTCTCGCCGTCGCATGATTGAGAGTAACCTGCGTCTGGTGGTAAAAATTGCCCG
CCGTTATGGCAATCGTGGACTGGCGTTGCTGGACCTGATTGAAGAGGGCAACCTGGGGCTTATCCGTGCAGTCGAGAAGT
TTGACCCGGAACGCGGGTTCCGCTTCTCAACATACGCAACCTGGTGGATTCGCCAGACAATCGAACGGGCGATTATGAAC
CAAACCCGTACGATTCGCTTGCCGATTCACATTGTTAAAGAGCTGAACGTATACCTGCGCACCGCACGTGAGTTGTCGCA
TAAACTGGACCACGAACCGAGTGCGGAAGAAATTGCAGAGCAACTGGATAAACCGGTTGATGACGTCAGCCGTATGCTTC
GTCTCAACGAGCGCATTACCTCGGTAGACACCCCGCTGGGCGGTGATTCCGAAAAAGCGTTGCTGGACATCCTGGCCGAT
GAAAAAGAGAACGGTCCGGAAGACACCACGCAAGATGACGATATGAAACAGAGCATCGTCAAATGGTTGTTCGAACTGAA
CGCCAAACAGCGTGAAGTGCTGGCGCGCCGTTTCGGTCTGCTGGGATATGAAGCTGCGACACTGGAAGATGTAGGCCGTG
AAATCGGTCTTACGCGTGAACGTGTTCGTCAGATTCAGGTTGAAGGCCTGCGGCCGTCTGCGCGAAATTCTGCAGACGCA
GGGGCTGAATATCGAAGCGCTGTTCCGCGAGTAAGTACCCTTGTCAAAAAAAGGCCAGTCGACAGACTGGCCTTTTTTTT
ACCGTTTGTCTTCTGCCAGCAACGGCGGGATACTCGGCACCATTGGCGCGTCATCAATATCTTTTTGCGTCATGCGAAAC
GCTTGTGGATAGTGTTCGCGGCTGGTACGGCGTAA

Upstream 100 bases:

>100_bases
AATCCGTAAACCCGCTGCGTTATTTACCGCAGCGATAAAGCGGCGGAACCAGGCTTTGACTTGCTAGTTCCGTCAAGGGA
TCACGGGTAGGAGCCACCTT

Downstream 100 bases:

>100_bases
CGGTTCGGTATCGCGCCAGGTATACAGACAATGCTGACACTGATATACCGTCCAGACTCCTTTTACCGGCGAAGTCGCCA
TGAGTTCAATATGTGCATCG

Product: RNA polymerase sigma factor RpoS

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 384; Mature: 383

Protein sequence:

>384_residues
MSQNTLKVHDLNEDAEFDENGVEAFDEKALSEEEPSDNDLAEEELLSQGATQRVLDATQLYLGEIGYSPLLTAEEEVYFA
RRALRGDVASRRRMIESNLRLVVKIARRYGNRGLALLDLIEEGNLGLIRAVEKFDPERGFRFSTYATWWIRQTIERAIMN
QTRTIRLPIHIVKELNVYLRTARELSHKLDHEPSAEEIAEQLDKPVDDVSRMLRLNERITSVDTPLGGDSEKALLDILAD
EKENGPEDTTQDDDMKQSIVKWLFELNAKQREVLARRFGLLGYEAATLEDVGREIGLTRERVRQIQVEGLRPSARNSADA
GAEYRSAVPRVSTLVKKRPVDRLAFFLPFVFCQQRRDTRHHWRVINIFLRHAKRLWIVFAAGTA

Sequences:

>Translated_384_residues
MSQNTLKVHDLNEDAEFDENGVEAFDEKALSEEEPSDNDLAEEELLSQGATQRVLDATQLYLGEIGYSPLLTAEEEVYFA
RRALRGDVASRRRMIESNLRLVVKIARRYGNRGLALLDLIEEGNLGLIRAVEKFDPERGFRFSTYATWWIRQTIERAIMN
QTRTIRLPIHIVKELNVYLRTARELSHKLDHEPSAEEIAEQLDKPVDDVSRMLRLNERITSVDTPLGGDSEKALLDILAD
EKENGPEDTTQDDDMKQSIVKWLFELNAKQREVLARRFGLLGYEAATLEDVGREIGLTRERVRQIQVEGLRPSARNSADA
GAEYRSAVPRVSTLVKKRPVDRLAFFLPFVFCQQRRDTRHHWRVINIFLRHAKRLWIVFAAGTA
>Mature_383_residues
SQNTLKVHDLNEDAEFDENGVEAFDEKALSEEEPSDNDLAEEELLSQGATQRVLDATQLYLGEIGYSPLLTAEEEVYFAR
RALRGDVASRRRMIESNLRLVVKIARRYGNRGLALLDLIEEGNLGLIRAVEKFDPERGFRFSTYATWWIRQTIERAIMNQ
TRTIRLPIHIVKELNVYLRTARELSHKLDHEPSAEEIAEQLDKPVDDVSRMLRLNERITSVDTPLGGDSEKALLDILADE
KENGPEDTTQDDDMKQSIVKWLFELNAKQREVLARRFGLLGYEAATLEDVGREIGLTRERVRQIQVEGLRPSARNSADAG
AEYRSAVPRVSTLVKKRPVDRLAFFLPFVFCQQRRDTRHHWRVINIFLRHAKRLWIVFAAGTA

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This sigma factor is required for the normal expression of the enterotoxin, yst, in the stationary phase [H]

COG id: COG0568

COG function: function code K; DNA-directed RNA polymerase, sigma subunit (sigma70/sigma32)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family [H]

Homologues:

Organism=Escherichia coli, GI1789098, Length=311, Percent_Identity=99.0353697749196, Blast_Score=618, Evalue=1e-178,
Organism=Escherichia coli, GI1789448, Length=244, Percent_Identity=41.8032786885246, Blast_Score=204, Evalue=8e-54,
Organism=Escherichia coli, GI1789871, Length=260, Percent_Identity=26.9230769230769, Blast_Score=87, Evalue=2e-18,

Paralogues:

None

Copy number: <10 (log phase) 250 (stationary phase) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR000943
- InterPro:   IPR009042
- InterPro:   IPR007627
- InterPro:   IPR007624
- InterPro:   IPR007630
- InterPro:   IPR013325
- InterPro:   IPR013324
- InterPro:   IPR012761
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00140 Sigma70_r1_2; PF04542 Sigma70_r2; PF04539 Sigma70_r3; PF04545 Sigma70_r4 [H]

EC number: NA

Molecular weight: Translated: 44111; Mature: 43979

Theoretical pI: Translated: 5.43; Mature: 5.43

Prosite motif: PS00715 SIGMA70_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQNTLKVHDLNEDAEFDENGVEAFDEKALSEEEPSDNDLAEEELLSQGATQRVLDATQL
CCCCCEEEEECCCCCCCCCCHHHHHHHHHCCCCCCCCCCHHHHHHHHCCHHHHHHHHHHH
YLGEIGYSPLLTAEEEVYFARRALRGDVASRRRMIESNLRLVVKIARRYGNRGLALLDLI
HHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEHHHH
EEGNLGLIRAVEKFDPERGFRFSTYATWWIRQTIERAIMNQTRTIRLPIHIVKELNVYLR
HCCCCHHHHHHHHCCCCCCCEEHHHHHHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHHH
TARELSHKLDHEPSAEEIAEQLDKPVDDVSRMLRLNERITSVDTPLGGDSEKALLDILAD
HHHHHHHHCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHC
EKENGPEDTTQDDDMKQSIVKWLFELNAKQREVLARRFGLLGYEAATLEDVGREIGLTRE
CCCCCCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCHH
RVRQIQVEGLRPSARNSADAGAEYRSAVPRVSTLVKKRPVDRLAFFLPFVFCQQRRDTRH
HHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCHHH
HWRVINIFLRHAKRLWIVFAAGTA
HHHHHHHHHHHHHHEEEEEECCCC
>Mature Secondary Structure 
SQNTLKVHDLNEDAEFDENGVEAFDEKALSEEEPSDNDLAEEELLSQGATQRVLDATQL
CCCCEEEEECCCCCCCCCCHHHHHHHHHCCCCCCCCCCHHHHHHHHCCHHHHHHHHHHH
YLGEIGYSPLLTAEEEVYFARRALRGDVASRRRMIESNLRLVVKIARRYGNRGLALLDLI
HHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEHHHH
EEGNLGLIRAVEKFDPERGFRFSTYATWWIRQTIERAIMNQTRTIRLPIHIVKELNVYLR
HCCCCHHHHHHHHCCCCCCCEEHHHHHHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHHH
TARELSHKLDHEPSAEEIAEQLDKPVDDVSRMLRLNERITSVDTPLGGDSEKALLDILAD
HHHHHHHHCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHC
EKENGPEDTTQDDDMKQSIVKWLFELNAKQREVLARRFGLLGYEAATLEDVGREIGLTRE
CCCCCCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCHH
RVRQIQVEGLRPSARNSADAGAEYRSAVPRVSTLVKKRPVDRLAFFLPFVFCQQRRDTRH
HHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCHHH
HWRVINIFLRHAKRLWIVFAAGTA
HHHHHHHHHHHHHHEEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7729893 [H]