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The map label for this gene is hydN [H]

Identifier: 29143104

GI number: 29143104

Start: 2828870

End: 2829415

Strand: Reverse

Name: hydN [H]

Synonym: t2745

Alternate gene names: 29143104

Gene position: 2829415-2828870 (Counterclockwise)

Preceding gene: 29143105

Following gene: 29143103

Centisome position: 59.05

GC content: 55.86

Gene sequence:

>546_bases
ATGAACCGTTTCATCATTGCAGACGCGAGTAAGTGCATTGGTTGCCGTACCTGTGAAGTGGCTTGCGTCGTATCCCATCA
GGAAAATCAGGATTGCGCATCGCTGACCCCTGAAACCTTTTTACCTCGTATCCATGTTATCAAAGGCGTGAACGTTTCCA
CGGCAACGCTGTGTCGCCAGTGTGAAGATGCGCCGTGCGCCAACGTTTGCCCGAATGGTGCCATCAGCCGTGACAAAGGC
TTTGTTCATGTGATGCAGGAACGCTGCATTGGCTGTAAAACTTGCGTAGTGGCATGTCCTTACGGCGCCATGGAAGTGGT
GGTTCGTCCGGTGGTTCGCAATAGCGGCGCGGGGCTAAACGTGCGTGCCGAAAAAGCCGAAGCCAACAAGTGCGATCTGT
GTCACCACCGTGAAGCCGGTCCGGCCTGTATGGCCGCTTGCCCAACGCACGCCCTCATCTGCGTGGACCGCAATAAGCTT
GAACAGCTGAGCGCGGAAAAACGCCGTCGTGCGGCGCTGGATTCAACGGCATCGCTGCTTTTCTGA

Upstream 100 bases:

>100_bases
CTCACGAAAATAGAAAGAATATTCTTAAATAACAATGAAATAAAAATTGGCACGATTCGTGTATATATCGGCACATCATC
TGTTATTGCTGGAGAATTTG

Downstream 100 bases:

>100_bases
TTTCTGCGATTGCGCGTAGAATACGCCACAAAGCATTGCCGGATGGCGGCATCAATGCCTTATCCGGTCTACGGTCCGTG
CGGTTGTAGGCCTGATAAGC

Product: electron transport protein HydN

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 181; Mature: 181

Protein sequence:

>181_residues
MNRFIIADASKCIGCRTCEVACVVSHQENQDCASLTPETFLPRIHVIKGVNVSTATLCRQCEDAPCANVCPNGAISRDKG
FVHVMQERCIGCKTCVVACPYGAMEVVVRPVVRNSGAGLNVRAEKAEANKCDLCHHREAGPACMAACPTHALICVDRNKL
EQLSAEKRRRAALDSTASLLF

Sequences:

>Translated_181_residues
MNRFIIADASKCIGCRTCEVACVVSHQENQDCASLTPETFLPRIHVIKGVNVSTATLCRQCEDAPCANVCPNGAISRDKG
FVHVMQERCIGCKTCVVACPYGAMEVVVRPVVRNSGAGLNVRAEKAEANKCDLCHHREAGPACMAACPTHALICVDRNKL
EQLSAEKRRRAALDSTASLLF
>Mature_181_residues
MNRFIIADASKCIGCRTCEVACVVSHQENQDCASLTPETFLPRIHVIKGVNVSTATLCRQCEDAPCANVCPNGAISRDKG
FVHVMQERCIGCKTCVVACPYGAMEVVVRPVVRNSGAGLNVRAEKAEANKCDLCHHREAGPACMAACPTHALICVDRNKL
EQLSAEKRRRAALDSTASLLF

Specific function: Electron transport from formate to hydrogen [H]

COG id: COG1142

COG function: function code C; Fe-S-cluster-containing hydrogenase components 2

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 4 4Fe-4S ferredoxin-type domains [H]

Homologues:

Organism=Escherichia coli, GI1789067, Length=173, Percent_Identity=95.9537572254335, Blast_Score=341, Evalue=2e-95,
Organism=Escherichia coli, GI2367245, Length=162, Percent_Identity=61.7283950617284, Blast_Score=195, Evalue=1e-51,
Organism=Escherichia coli, GI1788811, Length=172, Percent_Identity=47.093023255814, Blast_Score=154, Evalue=3e-39,
Organism=Escherichia coli, GI87082179, Length=167, Percent_Identity=39.5209580838323, Blast_Score=129, Evalue=1e-31,
Organism=Escherichia coli, GI87082180, Length=179, Percent_Identity=37.4301675977654, Blast_Score=127, Evalue=6e-31,
Organism=Escherichia coli, GI1789079, Length=187, Percent_Identity=40.1069518716578, Blast_Score=121, Evalue=2e-29,
Organism=Escherichia coli, GI87082114, Length=196, Percent_Identity=34.6938775510204, Blast_Score=101, Evalue=3e-23,
Organism=Escherichia coli, GI1787122, Length=193, Percent_Identity=33.6787564766839, Blast_Score=88, Evalue=4e-19,
Organism=Escherichia coli, GI1787872, Length=193, Percent_Identity=33.6787564766839, Blast_Score=88, Evalue=4e-19,
Organism=Escherichia coli, GI226510944, Length=158, Percent_Identity=35.4430379746835, Blast_Score=78, Evalue=4e-16,
Organism=Escherichia coli, GI2367345, Length=189, Percent_Identity=31.7460317460317, Blast_Score=74, Evalue=8e-15,
Organism=Escherichia coli, GI1787963, Length=174, Percent_Identity=28.735632183908, Blast_Score=63, Evalue=1e-11,
Organism=Escherichia coli, GI1789370, Length=205, Percent_Identity=27.3170731707317, Blast_Score=60, Evalue=7e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001450
- InterPro:   IPR017896
- InterPro:   IPR017900 [H]

Pfam domain/function: PF00037 Fer4 [H]

EC number: NA

Molecular weight: Translated: 19532; Mature: 19532

Theoretical pI: Translated: 7.96; Mature: 7.96

Prosite motif: PS00198 4FE4S_FERREDOXIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

9.9 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
12.2 %Cys+Met (Translated Protein)
9.9 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
12.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNRFIIADASKCIGCRTCEVACVVSHQENQDCASLTPETFLPRIHVIKGVNVSTATLCRQ
CCEEEEECCCHHCCCCCEEEEEEECCCCCCCHHHCCHHHHCCEEEEEECCCCHHHHHHHH
CEDAPCANVCPNGAISRDKGFVHVMQERCIGCKTCVVACPYGAMEVVVRPVVRNSGAGLN
HCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCHHEEECCCCHHHHHHHHHHHCCCCCCE
VRAEKAEANKCDLCHHREAGPACMAACPTHALICVDRNKLEQLSAEKRRRAALDSTASLL
EEECCCCCCCCCCCCCCCCCCHHHHHCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHC
F
C
>Mature Secondary Structure
MNRFIIADASKCIGCRTCEVACVVSHQENQDCASLTPETFLPRIHVIKGVNVSTATLCRQ
CCEEEEECCCHHCCCCCEEEEEEECCCCCCCHHHCCHHHHCCEEEEEECCCCHHHHHHHH
CEDAPCANVCPNGAISRDKGFVHVMQERCIGCKTCVVACPYGAMEVVVRPVVRNSGAGLN
HCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCHHEEECCCCHHHHHHHHHHHCCCCCCE
VRAEKAEANKCDLCHHREAGPACMAACPTHALICVDRNKLEQLSAEKRRRAALDSTASLL
EEECCCCCCCCCCCCCCCCCCHHHHHCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHC
F
C

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Fe [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]