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The map label for this gene is hypf [H]

Identifier: 29143103

GI number: 29143103

Start: 2826484

End: 2828724

Strand: Reverse

Name: hypf [H]

Synonym: t2744

Alternate gene names: 29143103

Gene position: 2828724-2826484 (Counterclockwise)

Preceding gene: 29143104

Following gene: 29143100

Centisome position: 59.03

GC content: 60.24

Gene sequence:

>2241_bases
ATGGCGATAGACACACCTTCCGGCGTACAGCTACGCATTCGGGGCAAAGTACAGGGCGTCGGTTTTCGTCCTTTTGTCTG
GCAGCTGGCGCAGCAGTTGCAATTACACGGCGACGTGTGTAATGACGGCGACGGCGTCGTCGTTCGGCTGCTGGAAGATC
CGTCGCAATTTATTGCCGCGCTCTATCAGGATTGCCCGCCGCTGGCGCGCATTGACAGCGTTGAACACGCGTCGTTGGTA
TGGGAGCGCGCACCGACGGATTTCACCATTCGTCAGAGCGCAGGCGGTTCGATGAACACGCAAATCGTGCCGGATGCGGC
GACCTGCCCGGCATGTCTTGCCGAGATGAATACCCCAGGCGAGCGGCGCTACCGTTATCCTTTCATCAATTGCACCCACT
GCGGACCACGCTTCACCATTATTCGCGCTATGCCCTATGACCGGCCATTTACGGTGATGGCGGCGTTTCCCTTGTGTCCG
GAATGCGACAGCGAATACCGCGATCCGTACGATCGCCGTTTTCATGCCCAGCCCGTTGCCTGTCCGTCATGCGGGCCGCA
TCTTGAGTGGCGGAGCCAACATGAACGAGCGGAAAAAGAGGCGGCTTTGCAGGCGGCGGTCGCCCAACTGAACGCCGGAG
GCATTATTGCCGTTAAAGGGCTGGGTGGTTTTCATCTGGCCTGCGACGCGCGCAACGATAACGCAGTGGCGATGCTGCGG
GCGCGTAAACATCGTCCGGCGAAACCATTGGCGGTGATGTTGCCCACGGCGCAAACGCTGCCGACCGCGGCGCGTTCGCT
GCTGACCACGCCAGCGGCCCCGATTGTACTGGTGGATAAGCAGTATGTACCTTCGCTGAGTGAGGGCATCGCGCCAGGAC
TTACGGAAGTGGGCGTGATGCTGCCAGCCAACCCATTGCAACATCTCTTGTTGCAGGCACTCAATTACCCGCTGGTGATG
ACATCCGGCAACCTGAGCGGCAAACCGCCCGCCATCACCAACGAACAGGCGCTGGACGATTTACACGATATTGCCGATGG
TTTTCTGTTGCACAATCGCGACATTGTACAGCGCATGGACGACTCTGTCGTGCGCGACAGCGGCGAAATGCTGCGTCGTT
CGCGGGGATACGTGCCGGACGCGATTGCGTTGCCGCCCGGATTTCGCGATGTGCCGCCGATACTTTGTCTGGGCGCGGAT
CTGAAAAACACGTTCTGTCTGGTACGCGGCGAACAGGCGGTTGTCAGCCAACATTTAGGCGATCTCAGCGATGACGGTAT
CCAGGCGCAGTGGCGCGAGGCATTGCGTTTGATCCAGTCAATCTACGATTTTACGCCAGAGCGTATCGTCTGTGATGCGC
ATCCGGGCTATGTTTCCAGTCAGTGGGCCAGTGAGATGCGTCTGCCGACAGAGACGGTGTTACACCATCATGCCCATGCG
GCGGCTTGCCTGGCCGAGCATGGTTGGCCGCTGGACGGCGGAGAGGTGATTGCCCTGACGGTAGACGGTATCGGCATGGG
TGAGAATGGCGCGCTATGGGGCGGAGAATGTTTGCGGGTCAATTATCGCGAATGCGAACATTTAGGCGGTTTACCCGCCG
TGGCGCTGCCGGGAGGCGATCTGGCTGCCAAACAGCCGTGGCGTAATCTGTTAGCGCAGTGCCTGCGCTTTGTGCCGGAC
TGGCAGAATTACCCGGAGACAGCGGGGCTGCAACAGCAAAACTGGAGTGTCCTGGCGCGCGCCATTGAGCGCGGCGTCAA
TGCCCCGTTGGCCTCTTCCTGCGGGCGGTTGTTTGACGCGGTGGCTGCCGCGCTTCGCTGCGCGCCAGCATCGCTTAGCT
ATGAGGGCGAGGCCGCCTGCGCGCTGGAGGCGCTGGCCTCTCAATGCGCTAACGTTGAGTATCCGGTAACGATGCCGCTT
AACGGCGCTCAACTGGACGTCGCTGTTTTCTGGCGGCAATGGTTGAACTGGCAGGCCACGCCCGCGCAACGCGCCTGGGC
TTTTCATGATGCGCTGGCGTGCGGGTTTGCCACGCTAATGCGCCAACAGGCTACGGCGCGGGGGATAACCACTCTGGTCT
TCAGCGGCGGGGTGATACACAACCGCTTACTGCGCGCGCGTCTTGCCTTTTATCTTTCTGATTTTAAATTGTTATTTCCG
CAGCGGTTACCGGCGGGCGACGGCGGACTGTCGTTTGGACAGGGCGTGATTGCCGCAGCGCGAGCGTTAAGGGAAGTGTA
G

Upstream 100 bases:

>100_bases
GCGGCATCAATGCCTTATCCGGTCTACGGTCCGTGCGGTTGTAGGCCTGATAAGCGTCGCGCCATCAGGCATCCGGGTTC
GATGATGGCGGGAAAATAAG

Downstream 100 bases:

>100_bases
GGCGCCGGATGGCGGCTGCGCCTTATCCGGCGTGTAAACAGATGCTCACTCGTAGGCCGGATAAGACGCGAAGCGGCGCT
ATCCGGCATCACAGGCTTAT

Product: hydrogenase maturation protein

Products: NA

Alternate protein names: Carbamoyl phosphate-converting enzyme hypF; [NiFe]-hydrogenase maturation factor hypF; Hydrogenase maturation protein hypF [H]

Number of amino acids: Translated: 746; Mature: 745

Protein sequence:

>746_residues
MAIDTPSGVQLRIRGKVQGVGFRPFVWQLAQQLQLHGDVCNDGDGVVVRLLEDPSQFIAALYQDCPPLARIDSVEHASLV
WERAPTDFTIRQSAGGSMNTQIVPDAATCPACLAEMNTPGERRYRYPFINCTHCGPRFTIIRAMPYDRPFTVMAAFPLCP
ECDSEYRDPYDRRFHAQPVACPSCGPHLEWRSQHERAEKEAALQAAVAQLNAGGIIAVKGLGGFHLACDARNDNAVAMLR
ARKHRPAKPLAVMLPTAQTLPTAARSLLTTPAAPIVLVDKQYVPSLSEGIAPGLTEVGVMLPANPLQHLLLQALNYPLVM
TSGNLSGKPPAITNEQALDDLHDIADGFLLHNRDIVQRMDDSVVRDSGEMLRRSRGYVPDAIALPPGFRDVPPILCLGAD
LKNTFCLVRGEQAVVSQHLGDLSDDGIQAQWREALRLIQSIYDFTPERIVCDAHPGYVSSQWASEMRLPTETVLHHHAHA
AACLAEHGWPLDGGEVIALTVDGIGMGENGALWGGECLRVNYRECEHLGGLPAVALPGGDLAAKQPWRNLLAQCLRFVPD
WQNYPETAGLQQQNWSVLARAIERGVNAPLASSCGRLFDAVAAALRCAPASLSYEGEAACALEALASQCANVEYPVTMPL
NGAQLDVAVFWRQWLNWQATPAQRAWAFHDALACGFATLMRQQATARGITTLVFSGGVIHNRLLRARLAFYLSDFKLLFP
QRLPAGDGGLSFGQGVIAAARALREV

Sequences:

>Translated_746_residues
MAIDTPSGVQLRIRGKVQGVGFRPFVWQLAQQLQLHGDVCNDGDGVVVRLLEDPSQFIAALYQDCPPLARIDSVEHASLV
WERAPTDFTIRQSAGGSMNTQIVPDAATCPACLAEMNTPGERRYRYPFINCTHCGPRFTIIRAMPYDRPFTVMAAFPLCP
ECDSEYRDPYDRRFHAQPVACPSCGPHLEWRSQHERAEKEAALQAAVAQLNAGGIIAVKGLGGFHLACDARNDNAVAMLR
ARKHRPAKPLAVMLPTAQTLPTAARSLLTTPAAPIVLVDKQYVPSLSEGIAPGLTEVGVMLPANPLQHLLLQALNYPLVM
TSGNLSGKPPAITNEQALDDLHDIADGFLLHNRDIVQRMDDSVVRDSGEMLRRSRGYVPDAIALPPGFRDVPPILCLGAD
LKNTFCLVRGEQAVVSQHLGDLSDDGIQAQWREALRLIQSIYDFTPERIVCDAHPGYVSSQWASEMRLPTETVLHHHAHA
AACLAEHGWPLDGGEVIALTVDGIGMGENGALWGGECLRVNYRECEHLGGLPAVALPGGDLAAKQPWRNLLAQCLRFVPD
WQNYPETAGLQQQNWSVLARAIERGVNAPLASSCGRLFDAVAAALRCAPASLSYEGEAACALEALASQCANVEYPVTMPL
NGAQLDVAVFWRQWLNWQATPAQRAWAFHDALACGFATLMRQQATARGITTLVFSGGVIHNRLLRARLAFYLSDFKLLFP
QRLPAGDGGLSFGQGVIAAARALREV
>Mature_745_residues
AIDTPSGVQLRIRGKVQGVGFRPFVWQLAQQLQLHGDVCNDGDGVVVRLLEDPSQFIAALYQDCPPLARIDSVEHASLVW
ERAPTDFTIRQSAGGSMNTQIVPDAATCPACLAEMNTPGERRYRYPFINCTHCGPRFTIIRAMPYDRPFTVMAAFPLCPE
CDSEYRDPYDRRFHAQPVACPSCGPHLEWRSQHERAEKEAALQAAVAQLNAGGIIAVKGLGGFHLACDARNDNAVAMLRA
RKHRPAKPLAVMLPTAQTLPTAARSLLTTPAAPIVLVDKQYVPSLSEGIAPGLTEVGVMLPANPLQHLLLQALNYPLVMT
SGNLSGKPPAITNEQALDDLHDIADGFLLHNRDIVQRMDDSVVRDSGEMLRRSRGYVPDAIALPPGFRDVPPILCLGADL
KNTFCLVRGEQAVVSQHLGDLSDDGIQAQWREALRLIQSIYDFTPERIVCDAHPGYVSSQWASEMRLPTETVLHHHAHAA
ACLAEHGWPLDGGEVIALTVDGIGMGENGALWGGECLRVNYRECEHLGGLPAVALPGGDLAAKQPWRNLLAQCLRFVPDW
QNYPETAGLQQQNWSVLARAIERGVNAPLASSCGRLFDAVAAALRCAPASLSYEGEAACALEALASQCANVEYPVTMPLN
GAQLDVAVFWRQWLNWQATPAQRAWAFHDALACGFATLMRQQATARGITTLVFSGGVIHNRLLRARLAFYLSDFKLLFPQ
RLPAGDGGLSFGQGVIAAARALREV

Specific function: Along with hypE, it catalyzes the synthesis of the CN ligands of the active site iron of [NiFe]-hydrogenases using carbamoylphosphate as a substrate. It functions as a carbamoyl transferase using carbamoylphosphate as a substrate and transferring the carb

COG id: COG0068

COG function: function code O; Hydrogenase maturation factor

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 YrdC-like domain [H]

Homologues:

Organism=Escherichia coli, GI2367152, Length=743, Percent_Identity=84.5222072678331, Blast_Score=1210, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001792
- InterPro:   IPR017968
- InterPro:   IPR004421
- InterPro:   IPR017945
- InterPro:   IPR006070
- InterPro:   IPR011125 [H]

Pfam domain/function: PF00708 Acylphosphatase; PF01300 Sua5_yciO_yrdC; PF07503 zf-HYPF [H]

EC number: NA

Molecular weight: Translated: 81177; Mature: 81046

Theoretical pI: Translated: 6.46; Mature: 6.46

Prosite motif: PS00150 ACYLPHOSPHATASE_1 ; PS51160 ACYLPHOSPHATASE_3 ; PS51163 YRDC

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.1 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
3.1 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAIDTPSGVQLRIRGKVQGVGFRPFVWQLAQQLQLHGDVCNDGDGVVVRLLEDPSQFIAA
CCCCCCCCCEEEEEEEECCCCCCHHHHHHHHHHHHCCCCCCCCCCEEEEEECCHHHHHHH
LYQDCPPLARIDSVEHASLVWERAPTDFTIRQSAGGSMNTQIVPDAATCPACLAEMNTPG
HHHCCCCHHHHCCCHHHHHHHHCCCCCEEEECCCCCCCCCEECCCCHHHHHHHHHCCCCC
ERRYRYPFINCTHCGPRFTIIRAMPYDRPFTVMAAFPLCPECDSEYRDPYDRRFHAQPVA
CCEEECCEEEEECCCCCEEEEEECCCCCCEEEEEECCCCCCCCHHHCCCHHHCCCCCCCC
CPSCGPHLEWRSQHERAEKEAALQAAVAQLNAGGIIAVKGLGGFHLACDARNDNAVAMLR
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCEEEEEECCCCCEEEEEE
ARKHRPAKPLAVMLPTAQTLPTAARSLLTTPAAPIVLVDKQYVPSLSEGIAPGLTEVGVM
HHCCCCCCCEEEEECCCHHHHHHHHHHHCCCCCCEEEECCHHCCCHHHCCCCCHHHHCEE
LPANPLQHLLLQALNYPLVMTSGNLSGKPPAITNEQALDDLHDIADGFLLHNRDIVQRMD
CCCCHHHHHHHHHCCCCEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHEECCHHHHHHHH
DSVVRDSGEMLRRSRGYVPDAIALPPGFRDVPPILCLGADLKNTFCLVRGEQAVVSQHLG
HHHHHCHHHHHHHHCCCCCCCCCCCCCCCCCCCEEEECCCCCCCEEEEECCHHHHHHHCC
DLSDDGIQAQWREALRLIQSIYDFTPERIVCDAHPGYVSSQWASEMRLPTETVLHHHAHA
CCCCCCCHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCHHHHHHHHCCCHHHHHHHHHHH
AACLAEHGWPLDGGEVIALTVDGIGMGENGALWGGECLRVNYRECEHLGGLPAVALPGGD
HHHHHHCCCCCCCCCEEEEEECCCCCCCCCCEECCCEEEECHHHHHHCCCCCEEEECCCC
LAAKQPWRNLLAQCLRFVPDWQNYPETAGLQQQNWSVLARAIERGVNAPLASSCGRLFDA
CCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHH
VAAALRCAPASLSYEGEAACALEALASQCANVEYPVTMPLNGAQLDVAVFWRQWLNWQAT
HHHHHHCCCCCCCCCCCHHHHHHHHHHHHCCCCCCEEECCCCCEEHHHHHHHHHCCCCCC
PAQRAWAFHDALACGFATLMRQQATARGITTLVFSGGVIHNRLLRARLAFYLSDFKLLFP
CHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHCC
QRLPAGDGGLSFGQGVIAAARALREV
CCCCCCCCCCCHHHHHHHHHHHHHCC
>Mature Secondary Structure 
AIDTPSGVQLRIRGKVQGVGFRPFVWQLAQQLQLHGDVCNDGDGVVVRLLEDPSQFIAA
CCCCCCCCEEEEEEEECCCCCCHHHHHHHHHHHHCCCCCCCCCCEEEEEECCHHHHHHH
LYQDCPPLARIDSVEHASLVWERAPTDFTIRQSAGGSMNTQIVPDAATCPACLAEMNTPG
HHHCCCCHHHHCCCHHHHHHHHCCCCCEEEECCCCCCCCCEECCCCHHHHHHHHHCCCCC
ERRYRYPFINCTHCGPRFTIIRAMPYDRPFTVMAAFPLCPECDSEYRDPYDRRFHAQPVA
CCEEECCEEEEECCCCCEEEEEECCCCCCEEEEEECCCCCCCCHHHCCCHHHCCCCCCCC
CPSCGPHLEWRSQHERAEKEAALQAAVAQLNAGGIIAVKGLGGFHLACDARNDNAVAMLR
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCEEEEEECCCCCEEEEEE
ARKHRPAKPLAVMLPTAQTLPTAARSLLTTPAAPIVLVDKQYVPSLSEGIAPGLTEVGVM
HHCCCCCCCEEEEECCCHHHHHHHHHHHCCCCCCEEEECCHHCCCHHHCCCCCHHHHCEE
LPANPLQHLLLQALNYPLVMTSGNLSGKPPAITNEQALDDLHDIADGFLLHNRDIVQRMD
CCCCHHHHHHHHHCCCCEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHEECCHHHHHHHH
DSVVRDSGEMLRRSRGYVPDAIALPPGFRDVPPILCLGADLKNTFCLVRGEQAVVSQHLG
HHHHHCHHHHHHHHCCCCCCCCCCCCCCCCCCCEEEECCCCCCCEEEEECCHHHHHHHCC
DLSDDGIQAQWREALRLIQSIYDFTPERIVCDAHPGYVSSQWASEMRLPTETVLHHHAHA
CCCCCCCHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCHHHHHHHHCCCHHHHHHHHHHH
AACLAEHGWPLDGGEVIALTVDGIGMGENGALWGGECLRVNYRECEHLGGLPAVALPGGD
HHHHHHCCCCCCCCCEEEEEECCCCCCCCCCEECCCEEEECHHHHHHCCCCCEEEECCCC
LAAKQPWRNLLAQCLRFVPDWQNYPETAGLQQQNWSVLARAIERGVNAPLASSCGRLFDA
CCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHH
VAAALRCAPASLSYEGEAACALEALASQCANVEYPVTMPLNGAQLDVAVFWRQWLNWQAT
HHHHHHCCCCCCCCCCCHHHHHHHHHHHHCCCCCCEEECCCCCEEHHHHHHHHHCCCCCC
PAQRAWAFHDALACGFATLMRQQATARGITTLVFSGGVIHNRLLRARLAFYLSDFKLLFP
CHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHCC
QRLPAGDGGLSFGQGVIAAARALREV
CCCCCCCCCCCHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9278503; 8661925; 12206761 [H]