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The map label for this gene is ygaA [H]

Identifier: 29143100

GI number: 29143100

Start: 2822112

End: 2823632

Strand: Reverse

Name: ygaA [H]

Synonym: t2741

Alternate gene names: 29143100

Gene position: 2823632-2822112 (Counterclockwise)

Preceding gene: 29143103

Following gene: 29143091

Centisome position: 58.92

GC content: 59.43

Gene sequence:

>1521_bases
ATGAGTTTTTCCGTGGAGGTATTGGCGGGCATCGCCATTGAGTTACAACGCGGCATTGGGCATCAGGATCGTTTTCAGCG
GCTGATTACTACGCTGCGTCAGGTGCTGGCGTGCGACGCCTCGGCGCTGCTGCGTTATGAATCACGACAATTTATTCCAC
TGGCGATTGATGGCCTGGCGCAGGACGTCCTCGGCAGACGTTTTACCCTGGAAGGGCATCCGCGCCTGGAAGCCATCGCC
CGCGCGGGGGACGTGGTGCGTTTTCCGGCTGATAGCGATTTACCCGATCCATATGACGGGTTAATTCCCGGACAGGAAAG
CCTGAAAGTCCACGCCTGTGTTGGTCTGCCGCTGTTTGCCGGACAGAACCTCATCGGTGCGCTGACCCTTGATGCTATGA
CGCCGGAGCAGTTCGAGGTATTTAGCGATGAAGAACTACGGCTTGTCGCCGCGCTGGCCGCCGGGGCATTGAGTAATGCG
CTGTTAATTGAACAACTGGAAAGCCAGAACATGCTGCCGGGGTCGTCAGGCGTGTTTGAACCGATAAAAGAGACGCACAT
GATCGGTCTGTCGCCGGCGATGACGCAGTTGAAAAAAGAGATCGAGATTGTGGCGGGCTCCGATCTTAACGTGCTGATCG
GCGGTGAAACGGGGACCGGTAAAGAGCTGGTGGCGAAAGCTATTCACCAGGGCTCCCCCAGAGCGGTCAATCCGCTGGTC
TATCTCAACTGCGCCGCGCTGCCGGAAAGCGTGGCGGAGAGCGAGTTGTTTGGTCATGTAAAAGGGGCTTTTACCGGGGC
TATCAGTAATCGTAGCGGTAAGTTTGAAATGGCGGATAACGGTACGCTGTTTCTGGATGAGATTGGCGAACTGTCGCTGG
CGCTACAGGCCAAGCTACTTCGCGTGTTGCAGTATGGCGATATTCAGCGCGTCGGCGACGATCGTAGCCTGCGCGTGGAT
GTGCGGGTGTTGGCGGCGACCAACCGTGACCTGCGTGAAGAGGTGCTGGCGGGGCGTTTTCGCGCCGACCTGTTCCATCG
CCTGAGCGTTTTCCCGCTCTTTGTGCCGCCGCTGCGCGAGCGCGGCGATGATGTGGTGTTGCTGGCGGGCTATTTTTGCG
AGCAGTGCCGACTGCGGCTGGGGCTGTCCCGCGTGGTACTGAGTCCCGGCGCGCGCCGCCATTTGCTCAACTATGGCTGG
CCGGGCAACGTCCGGGAGCTGGAACATGCCATTCACCGTGCGGTTGTACTGGCGAGAGCGACCCGGGCAGGGGATGAGGT
GGTACTGGAGGAGCAGCATTTTGCCTTGTCAGAGGACGTTTTACCCGCGCCGTCTGCGGAAAGCTTTCTGGCGTTGCCGG
CCTGCCGCAATTTGCGCGAATCGACGGAGAACTTCCAGCGAGAGATGATTCGCCAGGCGCTGGCGCAAAATAATCACAAC
TGGGCGGCCAGTGCGCGGGCGCTGGAAACCGACGTCGCCAACCTGCATCGGCTGGCGAAGCGTCTGGGACTGAAGGATTA
G

Upstream 100 bases:

>100_bases
TGAAATTATAATAATTTTGTATTTTTGGTATAGTCAAAAAGACTATAAATGATGCTGTCAATATGACACTATAGATTGTC
AAAATGACAATGAGGTAACC

Downstream 100 bases:

>100_bases
ATAATCCCCGCCTGGTAGAAATCCTGCAGGTTAATGGCGCCGGTGAGTTTGCCGTTTTCATCGACCACTGGCGCGGCGGT
AATTTTGCGTTTCATCAGGA

Product: anaerobic nitric oxide reductase transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 506; Mature: 505

Protein sequence:

>506_residues
MSFSVEVLAGIAIELQRGIGHQDRFQRLITTLRQVLACDASALLRYESRQFIPLAIDGLAQDVLGRRFTLEGHPRLEAIA
RAGDVVRFPADSDLPDPYDGLIPGQESLKVHACVGLPLFAGQNLIGALTLDAMTPEQFEVFSDEELRLVAALAAGALSNA
LLIEQLESQNMLPGSSGVFEPIKETHMIGLSPAMTQLKKEIEIVAGSDLNVLIGGETGTGKELVAKAIHQGSPRAVNPLV
YLNCAALPESVAESELFGHVKGAFTGAISNRSGKFEMADNGTLFLDEIGELSLALQAKLLRVLQYGDIQRVGDDRSLRVD
VRVLAATNRDLREEVLAGRFRADLFHRLSVFPLFVPPLRERGDDVVLLAGYFCEQCRLRLGLSRVVLSPGARRHLLNYGW
PGNVRELEHAIHRAVVLARATRAGDEVVLEEQHFALSEDVLPAPSAESFLALPACRNLRESTENFQREMIRQALAQNNHN
WAASARALETDVANLHRLAKRLGLKD

Sequences:

>Translated_506_residues
MSFSVEVLAGIAIELQRGIGHQDRFQRLITTLRQVLACDASALLRYESRQFIPLAIDGLAQDVLGRRFTLEGHPRLEAIA
RAGDVVRFPADSDLPDPYDGLIPGQESLKVHACVGLPLFAGQNLIGALTLDAMTPEQFEVFSDEELRLVAALAAGALSNA
LLIEQLESQNMLPGSSGVFEPIKETHMIGLSPAMTQLKKEIEIVAGSDLNVLIGGETGTGKELVAKAIHQGSPRAVNPLV
YLNCAALPESVAESELFGHVKGAFTGAISNRSGKFEMADNGTLFLDEIGELSLALQAKLLRVLQYGDIQRVGDDRSLRVD
VRVLAATNRDLREEVLAGRFRADLFHRLSVFPLFVPPLRERGDDVVLLAGYFCEQCRLRLGLSRVVLSPGARRHLLNYGW
PGNVRELEHAIHRAVVLARATRAGDEVVLEEQHFALSEDVLPAPSAESFLALPACRNLRESTENFQREMIRQALAQNNHN
WAASARALETDVANLHRLAKRLGLKD
>Mature_505_residues
SFSVEVLAGIAIELQRGIGHQDRFQRLITTLRQVLACDASALLRYESRQFIPLAIDGLAQDVLGRRFTLEGHPRLEAIAR
AGDVVRFPADSDLPDPYDGLIPGQESLKVHACVGLPLFAGQNLIGALTLDAMTPEQFEVFSDEELRLVAALAAGALSNAL
LIEQLESQNMLPGSSGVFEPIKETHMIGLSPAMTQLKKEIEIVAGSDLNVLIGGETGTGKELVAKAIHQGSPRAVNPLVY
LNCAALPESVAESELFGHVKGAFTGAISNRSGKFEMADNGTLFLDEIGELSLALQAKLLRVLQYGDIQRVGDDRSLRVDV
RVLAATNRDLREEVLAGRFRADLFHRLSVFPLFVPPLRERGDDVVLLAGYFCEQCRLRLGLSRVVLSPGARRHLLNYGWP
GNVRELEHAIHRAVVLARATRAGDEVVLEEQHFALSEDVLPAPSAESFLALPACRNLRESTENFQREMIRQALAQNNHNW
AASARALETDVANLHRLAKRLGLKD

Specific function: Required for the expression of anaerobic nitric oxide (NO) reductase, acts as a transcriptional activator for at least the norVW operon. Activation also requires sigma-54 [H]

COG id: COG3604

COG function: function code KT; Transcriptional regulator containing GAF, AAA-type ATPase, and DNA binding domains

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 sigma-54 factor interaction domain [H]

Homologues:

Organism=Escherichia coli, GI87082152, Length=507, Percent_Identity=87.9684418145957, Blast_Score=853, Evalue=0.0,
Organism=Escherichia coli, GI87082117, Length=316, Percent_Identity=48.7341772151899, Blast_Score=265, Evalue=5e-72,
Organism=Escherichia coli, GI1789087, Length=357, Percent_Identity=41.7366946778711, Blast_Score=259, Evalue=3e-70,
Organism=Escherichia coli, GI1790437, Length=302, Percent_Identity=48.0132450331126, Blast_Score=255, Evalue=4e-69,
Organism=Escherichia coli, GI1788550, Length=309, Percent_Identity=44.6601941747573, Blast_Score=247, Evalue=1e-66,
Organism=Escherichia coli, GI1790299, Length=336, Percent_Identity=42.5595238095238, Blast_Score=237, Evalue=1e-63,
Organism=Escherichia coli, GI1788905, Length=315, Percent_Identity=39.6825396825397, Blast_Score=223, Evalue=2e-59,
Organism=Escherichia coli, GI1789233, Length=322, Percent_Identity=39.4409937888199, Blast_Score=218, Evalue=6e-58,
Organism=Escherichia coli, GI87081872, Length=322, Percent_Identity=39.7515527950311, Blast_Score=212, Evalue=4e-56,
Organism=Escherichia coli, GI1786524, Length=248, Percent_Identity=46.3709677419355, Blast_Score=196, Evalue=4e-51,
Organism=Escherichia coli, GI1787583, Length=232, Percent_Identity=43.9655172413793, Blast_Score=191, Evalue=7e-50,
Organism=Escherichia coli, GI1789828, Length=251, Percent_Identity=37.4501992031873, Blast_Score=141, Evalue=9e-35,
Organism=Escherichia coli, GI87081858, Length=207, Percent_Identity=34.7826086956522, Blast_Score=112, Evalue=6e-26,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR003018
- InterPro:   IPR009057
- InterPro:   IPR002078 [H]

Pfam domain/function: PF01590 GAF; PF00158 Sigma54_activat [H]

EC number: NA

Molecular weight: Translated: 55379; Mature: 55248

Theoretical pI: Translated: 5.79; Mature: 5.79

Prosite motif: PS00675 SIGMA54_INTERACT_1 ; PS00676 SIGMA54_INTERACT_2 ; PS00688 SIGMA54_INTERACT_3 ; PS50045 SIGMA54_INTERACT_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSFSVEVLAGIAIELQRGIGHQDRFQRLITTLRQVLACDASALLRYESRQFIPLAIDGLA
CCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCHHHHHHHCCCCEEEEHHHHHH
QDVLGRRFTLEGHPRLEAIARAGDVVRFPADSDLPDPYDGLIPGQESLKVHACVGLPLFA
HHHHCCEEEECCCCHHHHHHHCCCEEECCCCCCCCCCCCCCCCCCCCCEEEEEECCHHHC
GQNLIGALTLDAMTPEQFEVFSDEELRLVAALAAGALSNALLIEQLESQNMLPGSSGVFE
CCCEEEEEEECCCCCHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHH
PIKETHMIGLSPAMTQLKKEIEIVAGSDLNVLIGGETGTGKELVAKAIHQGSPRAVNPLV
HHHHHHEECCCHHHHHHHHHHEEEECCCCEEEEECCCCCCHHHHHHHHHCCCCCCCCCEE
YLNCAALPESVAESELFGHVKGAFTGAISNRSGKFEMADNGTLFLDEIGELSLALQAKLL
EEEEHHCCHHHHHHHHHHHHHHHHEEEECCCCCCEEECCCCEEEEECHHHHHHHHHHHHH
RVLQYGDIQRVGDDRSLRVDVRVLAATNRDLREEVLAGRFRADLFHRLSVFPLFVPPLRE
HHHHCCCHHHCCCCCCEEEEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHEECCHHHC
RGDDVVLLAGYFCEQCRLRLGLSRVVLSPGARRHLLNYGWPGNVRELEHAIHRAVVLARA
CCCCEEEEHHHHHHHHHHHHCHHHHHHCCCHHHHHHCCCCCCCHHHHHHHHHHHHHHHHH
TRAGDEVVLEEQHFALSEDVLPAPSAESFLALPACRNLRESTENFQREMIRQALAQNNHN
HCCCCCEEEECHHHHCCCCCCCCCCCCCEEECHHHHHHHHHHHHHHHHHHHHHHHCCCCC
WAASARALETDVANLHRLAKRLGLKD
HHHHHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
SFSVEVLAGIAIELQRGIGHQDRFQRLITTLRQVLACDASALLRYESRQFIPLAIDGLA
CCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCHHHHHHHCCCCEEEEHHHHHH
QDVLGRRFTLEGHPRLEAIARAGDVVRFPADSDLPDPYDGLIPGQESLKVHACVGLPLFA
HHHHCCEEEECCCCHHHHHHHCCCEEECCCCCCCCCCCCCCCCCCCCCEEEEEECCHHHC
GQNLIGALTLDAMTPEQFEVFSDEELRLVAALAAGALSNALLIEQLESQNMLPGSSGVFE
CCCEEEEEEECCCCCHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHH
PIKETHMIGLSPAMTQLKKEIEIVAGSDLNVLIGGETGTGKELVAKAIHQGSPRAVNPLV
HHHHHHEECCCHHHHHHHHHHEEEECCCCEEEEECCCCCCHHHHHHHHHCCCCCCCCCEE
YLNCAALPESVAESELFGHVKGAFTGAISNRSGKFEMADNGTLFLDEIGELSLALQAKLL
EEEEHHCCHHHHHHHHHHHHHHHHEEEECCCCCCEEECCCCEEEEECHHHHHHHHHHHHH
RVLQYGDIQRVGDDRSLRVDVRVLAATNRDLREEVLAGRFRADLFHRLSVFPLFVPPLRE
HHHHCCCHHHCCCCCCEEEEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHEECCHHHC
RGDDVVLLAGYFCEQCRLRLGLSRVVLSPGARRHLLNYGWPGNVRELEHAIHRAVVLARA
CCCCEEEEHHHHHHHHHHHHCHHHHHHCCCHHHHHHCCCCCCCHHHHHHHHHHHHHHHHH
TRAGDEVVLEEQHFALSEDVLPAPSAESFLALPACRNLRESTENFQREMIRQALAQNNHN
HCCCCCEEEECHHHHCCCCCCCCCCCCCEEECHHHHHHHHHHHHHHHHHHHHHHHCCCCC
WAASARALETDVANLHRLAKRLGLKD
HHHHHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA