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The map label for this gene is tgt [H]

Identifier: 29142846

GI number: 29142846

Start: 2526947

End: 2528074

Strand: Reverse

Name: tgt [H]

Synonym: t2458

Alternate gene names: 29142846

Gene position: 2528074-2526947 (Counterclockwise)

Preceding gene: 29142847

Following gene: 29142845

Centisome position: 52.76

GC content: 52.57

Gene sequence:

>1128_bases
ATGAAATTTGAGTTAGATACCACCGATGGTCGCGCGCGTCGCGGTCGTCTGGTGTTTGATCGTGGCGTAGTGGAAACGCC
TGCTTTTATGCCTGTGGGTACTTACGGTACGGTAAAAGGCATGACGCCGGAAGAAGTGGAAGCGACCGGCGCGCAGATTA
TTCTCGGCAACACTTTCCACCTGTGGCTGCGCCCGGGTCAGGAAATCATGAAGCTGCACGGCGACCTGCACGATTTCATG
CAGTGGAAAGGGCCGATTCTCACCGATTCCGGCGGCTTCCAGGTCTTCAGCCTCGGCGATATTCGTAAGATTACCGAGCA
AGGCGTGCACTTCCGTAACCCGATCAACGGCGATCCGATTTTCCTCGATCCGGAAAAGTCGATGGAGATCCAGTACGATC
TCGGTTCCGATATCGTGATGATCTTTGATGAATGTACGCCGTACCCGGCAGACTGGGACTATGCTAAACGCTCGATGGAG
ATGTCGTTGCGTTGGGCGAAGCGTAGCCGCGACCGTTTTGATAGCCTTGGCAATAAGAATGCGCTTTTTGGCATCATTCA
GGGCAGCGTTTACGAAGATTTACGCGATATCTCGGTGAAAGGTCTGGTAGAGATTGGCTTTGATGGCTACGCTGTCGGCG
GTTTGGCTGTCGGTGAGCCGAAAGCAGATATGCACCGCATTCTGGAGCACGTTTGCCCGCAGATCCCGGCTGACAAACCG
CGTTACCTGATGGGCGTGGGTAAACCGGAAGATCTGGTGGAAGGGGTGCGTCGCGGTATTGATATGTTTGACTGTGTCAT
GCCAACGCGAAATGCCCGTAATGGTCATCTGTTTGTGACCGACGGCGTGGTGAAGATTCGTAATGCAAAGCATAAAAGCG
ACATCAGCCCGCTTGACGCCGAGTGCGATTGCTACACCTGTCGCAATTATTCACGCGCTTACTTGCATCATCTCGACCGT
TGCAACGAAATATTAGGTGCGCGTCTCAATACCATTCATAACTTGCGTTACTACCAGCGTTTGATGGCGGGTTTACGCAA
GGCTATTGAAGAGGGTAAATTAGAGAGCTTCGTAACCGAGTTTTACCAACGTCAGGGGCGACCCGTTCCACCTTTGAACG
TTGATTAA

Upstream 100 bases:

>100_bases
ACTGGCTGCGCCGATGACGCCTTCGGGAATTTATTAGCTATGGAATCATTCCGTAGCGAGTCGTTAAACGTCGGACTGTT
TTTCTGACGCAGGAGTGATA

Downstream 100 bases:

>100_bases
TTTTAATAATGAGGGAATTTGAATGAGCTTTTTTATTTCTGATGCGGTAGCGGCAACAGGTGCTCCGGCGCAGGGCAGCC
CGATGTCTCTGATTTTAATG

Product: queuine tRNA-ribosyltransferase

Products: NA

Alternate protein names: Guanine insertion enzyme; tRNA-guanine transglycosylase [H]

Number of amino acids: Translated: 375; Mature: 375

Protein sequence:

>375_residues
MKFELDTTDGRARRGRLVFDRGVVETPAFMPVGTYGTVKGMTPEEVEATGAQIILGNTFHLWLRPGQEIMKLHGDLHDFM
QWKGPILTDSGGFQVFSLGDIRKITEQGVHFRNPINGDPIFLDPEKSMEIQYDLGSDIVMIFDECTPYPADWDYAKRSME
MSLRWAKRSRDRFDSLGNKNALFGIIQGSVYEDLRDISVKGLVEIGFDGYAVGGLAVGEPKADMHRILEHVCPQIPADKP
RYLMGVGKPEDLVEGVRRGIDMFDCVMPTRNARNGHLFVTDGVVKIRNAKHKSDISPLDAECDCYTCRNYSRAYLHHLDR
CNEILGARLNTIHNLRYYQRLMAGLRKAIEEGKLESFVTEFYQRQGRPVPPLNVD

Sequences:

>Translated_375_residues
MKFELDTTDGRARRGRLVFDRGVVETPAFMPVGTYGTVKGMTPEEVEATGAQIILGNTFHLWLRPGQEIMKLHGDLHDFM
QWKGPILTDSGGFQVFSLGDIRKITEQGVHFRNPINGDPIFLDPEKSMEIQYDLGSDIVMIFDECTPYPADWDYAKRSME
MSLRWAKRSRDRFDSLGNKNALFGIIQGSVYEDLRDISVKGLVEIGFDGYAVGGLAVGEPKADMHRILEHVCPQIPADKP
RYLMGVGKPEDLVEGVRRGIDMFDCVMPTRNARNGHLFVTDGVVKIRNAKHKSDISPLDAECDCYTCRNYSRAYLHHLDR
CNEILGARLNTIHNLRYYQRLMAGLRKAIEEGKLESFVTEFYQRQGRPVPPLNVD
>Mature_375_residues
MKFELDTTDGRARRGRLVFDRGVVETPAFMPVGTYGTVKGMTPEEVEATGAQIILGNTFHLWLRPGQEIMKLHGDLHDFM
QWKGPILTDSGGFQVFSLGDIRKITEQGVHFRNPINGDPIFLDPEKSMEIQYDLGSDIVMIFDECTPYPADWDYAKRSME
MSLRWAKRSRDRFDSLGNKNALFGIIQGSVYEDLRDISVKGLVEIGFDGYAVGGLAVGEPKADMHRILEHVCPQIPADKP
RYLMGVGKPEDLVEGVRRGIDMFDCVMPTRNARNGHLFVTDGVVKIRNAKHKSDISPLDAECDCYTCRNYSRAYLHHLDR
CNEILGARLNTIHNLRYYQRLMAGLRKAIEEGKLESFVTEFYQRQGRPVPPLNVD

Specific function: Exchanges the guanine residue with 7-aminomethyl-7- deazaguanine in tRNAs with GU(N) anticodons (tRNA-Asp, -Asn, -His and -Tyr). After this exchange, a cyclopentendiol moiety is attached to the 7-aminomethyl group of 7-deazaguanine, resulting in the hyper

COG id: COG0343

COG function: function code J; Queuine/archaeosine tRNA-ribosyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the queuine tRNA-ribosyltransferase family [H]

Homologues:

Organism=Homo sapiens, GI13654276, Length=364, Percent_Identity=42.5824175824176, Blast_Score=299, Evalue=3e-81,
Organism=Escherichia coli, GI1786607, Length=375, Percent_Identity=97.6, Blast_Score=761, Evalue=0.0,
Organism=Caenorhabditis elegans, GI17544680, Length=362, Percent_Identity=41.1602209944751, Blast_Score=285, Evalue=4e-77,
Organism=Caenorhabditis elegans, GI25146502, Length=140, Percent_Identity=31.4285714285714, Blast_Score=86, Evalue=5e-17,
Organism=Drosophila melanogaster, GI19920510, Length=371, Percent_Identity=43.6657681940701, Blast_Score=307, Evalue=8e-84,
Organism=Drosophila melanogaster, GI21355311, Length=264, Percent_Identity=28.4090909090909, Blast_Score=93, Evalue=3e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004803
- InterPro:   IPR002616 [H]

Pfam domain/function: PF01702 TGT [H]

EC number: =2.4.2.29 [H]

Molecular weight: Translated: 42534; Mature: 42534

Theoretical pI: Translated: 6.59; Mature: 6.59

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
5.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKFELDTTDGRARRGRLVFDRGVVETPAFMPVGTYGTVKGMTPEEVEATGAQIILGNTFH
CEEECCCCCCCCCCCCEEEECCCCCCCCCCCCCCCCCCCCCCHHHHHHCCCEEEECCEEE
LWLRPGQEIMKLHGDLHDFMQWKGPILTDSGGFQVFSLGDIRKITEQGVHFRNPINGDPI
EEECCCHHHHHHHHHHHHHHHCCCCEEECCCCEEEEECCHHHHHHHCCCEECCCCCCCCE
FLDPEKSMEIQYDLGSDIVMIFDECTPYPADWDYAKRSMEMSLRWAKRSRDRFDSLGNKN
EECCCCCEEEEEECCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
ALFGIIQGSVYEDLRDISVKGLVEIGFDGYAVGGLAVGEPKADMHRILEHVCPQIPADKP
EEEEEECCHHHHHHHHCCHHEEEEECCCCEEECCEEECCCHHHHHHHHHHHCCCCCCCCC
RYLMGVGKPEDLVEGVRRGIDMFDCVMPTRNARNGHLFVTDGVVKIRNAKHKSDISPLDA
CEEEECCCHHHHHHHHHHCCCHHHHHCCCCCCCCCEEEEECCEEEECCCCCCCCCCCCCC
ECDCYTCRNYSRAYLHHLDRCNEILGARLNTIHNLRYYQRLMAGLRKAIEEGKLESFVTE
CCCEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHH
FYQRQGRPVPPLNVD
HHHHCCCCCCCCCCC
>Mature Secondary Structure
MKFELDTTDGRARRGRLVFDRGVVETPAFMPVGTYGTVKGMTPEEVEATGAQIILGNTFH
CEEECCCCCCCCCCCCEEEECCCCCCCCCCCCCCCCCCCCCCHHHHHHCCCEEEECCEEE
LWLRPGQEIMKLHGDLHDFMQWKGPILTDSGGFQVFSLGDIRKITEQGVHFRNPINGDPI
EEECCCHHHHHHHHHHHHHHHCCCCEEECCCCEEEEECCHHHHHHHCCCEECCCCCCCCE
FLDPEKSMEIQYDLGSDIVMIFDECTPYPADWDYAKRSMEMSLRWAKRSRDRFDSLGNKN
EECCCCCEEEEEECCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
ALFGIIQGSVYEDLRDISVKGLVEIGFDGYAVGGLAVGEPKADMHRILEHVCPQIPADKP
EEEEEECCHHHHHHHHCCHHEEEEECCCCEEECCEEECCCHHHHHHHHHHHCCCCCCCCC
RYLMGVGKPEDLVEGVRRGIDMFDCVMPTRNARNGHLFVTDGVVKIRNAKHKSDISPLDA
CEEEECCCHHHHHHHHHHCCCHHHHHCCCCCCCCCEEEEECCEEEECCCCCCCCCCCCCC
ECDCYTCRNYSRAYLHHLDRCNEILGARLNTIHNLRYYQRLMAGLRKAIEEGKLESFVTE
CCCEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHH
FYQRQGRPVPPLNVD
HHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA