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The map label for this gene is queA [H]

Identifier: 29142847

GI number: 29142847

Start: 2528271

End: 2529335

Strand: Reverse

Name: queA [H]

Synonym: t2459

Alternate gene names: 29142847

Gene position: 2529335-2528271 (Counterclockwise)

Preceding gene: 29142850

Following gene: 29142846

Centisome position: 52.78

GC content: 55.68

Gene sequence:

>1065_bases
ATGCGCGTTACCGATTTTTCCTTTGAACTACCCGAATCCTTGATTGCCCACTACCCACAGCCGGAACGCAGTCGCTGCCG
TTTGCTGTCATTAGAGGGGCCGACGGGCGCGCTGACGCACGGTACTTTCACCGATCTGCTCGATAAGCTCAACCCTGGCG
ATTTGCTGGTCTTCAATAATACCCGCGTGATCCCGGCGCGCCTGTTTGGCCGTAAAGCCAGCGGCGGCAAGATAGAAGTG
CTGGTCGAACGTATGCTCGATGATAAACGCATCCTGGCGCATATTCGCGCTTCTAAAGCGCCAAAACCGGGCACGGAACT
GCTACTGGGCGATGACGAAAGTATTCACGCGACCATGACCGCGCGTCACGGCGCTCTGTTTGAAGTTGAGTTTAATGACC
CGCGTCCGGTGCTGGATATTCTCAACGCCATCGGCCATATGCCGTTGCCGCCGTATATCGACCGTCCGGACGAGGACGCC
GACCGCGAACTGTATCAGACGGTTTACAGTGAAAAACCGGGCGCCGTCGCCGCGCCGACCGCGGGGCTGCACTTTGATGA
GCCGCTGCTGGCGGCGCTTCGTGAGAAAGGTGTCGAAATGGCGTTTGTTACGCTACACGTCGGCGCGGGCACTTTCCAGC
CAGTGCGTGTCGATACCATTGAAGATCACATCATGCACTCCGAATATGCTGAGGTGCCGCAAGAGGTGGTCGACGCGGTG
CTGGCGGCGAAAGCGCGCGGCAACCGGGTTATCGCCGTCGGCACGACCTCGGTACGCTCACTGGAGAGCGCGGCGCAGGC
GGCAAAAAGCGATTTGATAGAGCCATTCTTCGGCGATACGCAAATCTTTATTTATCCAGGTTATCAATACAAAGTGATTG
ATGCGCTGATCACCAACTTCCATCTGCCGGAATCGACGTTGATTATGCTGGTTTCCGCATTTGCCGGTTATCAACACACC
ATGAATGCCTATAAGACTGCGGTAGAACAAAAATATCGCTTTTTTAGCTACGGGGACGCGATGTTTATCACGTGCAATCC
GCAGGCTATTTCTGAGCGTCCGTAA

Upstream 100 bases:

>100_bases
AATTCATTATTGATTCTCTGTCCAAAAAGCGGGTAACGGTTGCAGCGGGAACGCCCCGGCACTAGACTACCCGCCTCTTA
TTTTAGTCTGAGTCAGAGTC

Downstream 100 bases:

>100_bases
GAAGGCTATCTCACTTGCCATTTTGACGCCGGGCAGTGCTCGCCATCCTCACGTACTGCGTGTACGCTCCGGTGGTTGTG
CGCTGGCCGACTCCAAACTG

Product: S-adenosylmethionine:tRNA ribosyltransferase-isomerase

Products: NA

Alternate protein names: Queuosine biosynthesis protein queA [H]

Number of amino acids: Translated: 354; Mature: 354

Protein sequence:

>354_residues
MRVTDFSFELPESLIAHYPQPERSRCRLLSLEGPTGALTHGTFTDLLDKLNPGDLLVFNNTRVIPARLFGRKASGGKIEV
LVERMLDDKRILAHIRASKAPKPGTELLLGDDESIHATMTARHGALFEVEFNDPRPVLDILNAIGHMPLPPYIDRPDEDA
DRELYQTVYSEKPGAVAAPTAGLHFDEPLLAALREKGVEMAFVTLHVGAGTFQPVRVDTIEDHIMHSEYAEVPQEVVDAV
LAAKARGNRVIAVGTTSVRSLESAAQAAKSDLIEPFFGDTQIFIYPGYQYKVIDALITNFHLPESTLIMLVSAFAGYQHT
MNAYKTAVEQKYRFFSYGDAMFITCNPQAISERP

Sequences:

>Translated_354_residues
MRVTDFSFELPESLIAHYPQPERSRCRLLSLEGPTGALTHGTFTDLLDKLNPGDLLVFNNTRVIPARLFGRKASGGKIEV
LVERMLDDKRILAHIRASKAPKPGTELLLGDDESIHATMTARHGALFEVEFNDPRPVLDILNAIGHMPLPPYIDRPDEDA
DRELYQTVYSEKPGAVAAPTAGLHFDEPLLAALREKGVEMAFVTLHVGAGTFQPVRVDTIEDHIMHSEYAEVPQEVVDAV
LAAKARGNRVIAVGTTSVRSLESAAQAAKSDLIEPFFGDTQIFIYPGYQYKVIDALITNFHLPESTLIMLVSAFAGYQHT
MNAYKTAVEQKYRFFSYGDAMFITCNPQAISERP
>Mature_354_residues
MRVTDFSFELPESLIAHYPQPERSRCRLLSLEGPTGALTHGTFTDLLDKLNPGDLLVFNNTRVIPARLFGRKASGGKIEV
LVERMLDDKRILAHIRASKAPKPGTELLLGDDESIHATMTARHGALFEVEFNDPRPVLDILNAIGHMPLPPYIDRPDEDA
DRELYQTVYSEKPGAVAAPTAGLHFDEPLLAALREKGVEMAFVTLHVGAGTFQPVRVDTIEDHIMHSEYAEVPQEVVDAV
LAAKARGNRVIAVGTTSVRSLESAAQAAKSDLIEPFFGDTQIFIYPGYQYKVIDALITNFHLPESTLIMLVSAFAGYQHT
MNAYKTAVEQKYRFFSYGDAMFITCNPQAISERP

Specific function: Transfers and isomerizes the ribose moiety from AdoMet to the 7-aminomethyl group of 7-deazaguanine (preQ1-tRNA) to give epoxyqueuosine (oQ-tRNA) [H]

COG id: COG0809

COG function: function code J; S-adenosylmethionine:tRNA-ribosyltransferase-isomerase (queuine synthetase)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the queA family [H]

Homologues:

Organism=Escherichia coli, GI1786606, Length=353, Percent_Identity=94.0509915014164, Blast_Score=684, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003699 [H]

Pfam domain/function: PF02547 Queuosine_synth [H]

EC number: 5.-.-.- [C]

Molecular weight: Translated: 39196; Mature: 39196

Theoretical pI: Translated: 5.40; Mature: 5.40

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRVTDFSFELPESLIAHYPQPERSRCRLLSLEGPTGALTHGTFTDLLDKLNPGDLLVFNN
CCCCCCCCCCCHHHHHCCCCCCCCCEEEEEECCCCCCCCCCHHHHHHHCCCCCCEEEECC
TRVIPARLFGRKASGGKIEVLVERMLDDKRILAHIRASKAPKPGTELLLGDDESIHATMT
CEEEEHHHHCCCCCCCHHHHHHHHHHCHHHHHHHHHHCCCCCCCCEEEECCCCCEEEEEE
ARHGALFEVEFNDPRPVLDILNAIGHMPLPPYIDRPDEDADRELYQTVYSEKPGAVAAPT
ECCCEEEEEEECCCCHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCEECCC
AGLHFDEPLLAALREKGVEMAFVTLHVGAGTFQPVRVDTIEDHIMHSEYAEVPQEVVDAV
CCCCCCCHHHHHHHHCCCEEEEEEEEECCCCCCCEEECHHHHHHHHHHHHHHHHHHHHHH
LAAKARGNRVIAVGTTSVRSLESAAQAAKSDLIEPFFGDTQIFIYPGYQYKVIDALITNF
HHHHCCCCEEEEEECHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCHHHHHHHHHHC
HLPESTLIMLVSAFAGYQHTMNAYKTAVEQKYRFFSYGDAMFITCNPQAISERP
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCEEEEEECCHHCCCCC
>Mature Secondary Structure
MRVTDFSFELPESLIAHYPQPERSRCRLLSLEGPTGALTHGTFTDLLDKLNPGDLLVFNN
CCCCCCCCCCCHHHHHCCCCCCCCCEEEEEECCCCCCCCCCHHHHHHHCCCCCCEEEECC
TRVIPARLFGRKASGGKIEVLVERMLDDKRILAHIRASKAPKPGTELLLGDDESIHATMT
CEEEEHHHHCCCCCCCHHHHHHHHHHCHHHHHHHHHHCCCCCCCCEEEECCCCCEEEEEE
ARHGALFEVEFNDPRPVLDILNAIGHMPLPPYIDRPDEDADRELYQTVYSEKPGAVAAPT
ECCCEEEEEEECCCCHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCEECCC
AGLHFDEPLLAALREKGVEMAFVTLHVGAGTFQPVRVDTIEDHIMHSEYAEVPQEVVDAV
CCCCCCCHHHHHHHHCCCEEEEEEEEECCCCCCCEEECHHHHHHHHHHHHHHHHHHHHHH
LAAKARGNRVIAVGTTSVRSLESAAQAAKSDLIEPFFGDTQIFIYPGYQYKVIDALITNF
HHHHCCCCEEEEEECHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCHHHHHHHHHHC
HLPESTLIMLVSAFAGYQHTMNAYKTAVEQKYRFFSYGDAMFITCNPQAISERP
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCEEEEEECCHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Isomerases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA