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The map label for this gene is dsbG [H]

Identifier: 29142661

GI number: 29142661

Start: 2326975

End: 2327721

Strand: Direct

Name: dsbG [H]

Synonym: t2261

Alternate gene names: 29142661

Gene position: 2326975-2327721 (Clockwise)

Preceding gene: 29142653

Following gene: 29142662

Centisome position: 48.56

GC content: 49.8

Gene sequence:

>747_bases
ATGATTAAACGTACATTGCTGCTTGCTATGCTGCCCATTCTCGCCCACGCGGAAGAACTGCCTGCGCTGGTTAAAGCCAT
TGAAAAACAGAGCATAACAATCCTGAAATCCTTCGAGGCGCCGGGGGGCATGAAGGGCTATCTCGGAAAGTATCAGGATA
TGGGCGTCACGATTTACCTGACGCCGGACGGTAAACACGCGATTTCCGGCTATATGTATAATGAAAAAGGTGAAAATCTT
AGCAACGCCCTGATAGAAAAAGAGATCTATGCGCCAGCCGGACGTGAAATGTGGCAGAAAATGGAAAAAGCGTCATGGAT
ACTCGACGGTAAAAAAGACGCGCCGGTGGTGCTGTATGTCTTTGCCGATCCTTTCTGCCCCTATTGTAAGCAGTTTTGGC
AACAAGCTCGTCCGTGGGTCGAATCCGGCAAGGTACAATTACGCACATTACTGGTCGGCGTGATTAAACCGGAAAGCCCG
GCCACAGCCGCAGCGATTTTAGCCGCGAAAAACCCGGCGAAAACCTGGCATGACTATGAAGCCTCCGCAGGAAAGATGAA
GCTGGAGGTTCCTGCGTCCATCCCCCCGGCGCAGATGAAAGTCATTAATCAGAATCAGCAGTTAATGGACGATCTTGGCG
CTAACGCGACGCCAGCAATTTATTACATGAATAAAGATAAGATTTTGCAACAGGTTGTTGGTTTACCGGAAAAAGCGCAA
CTGGACGCCATGATGGGGCAGCCTTAA

Upstream 100 bases:

>100_bases
CCAGCACAGAAAGGGCAATCCTGGCACGCCAGCTCTTACCTATGTCTGCGATAGGCATCATCATTAATACTCTTTTCGCT
TTGATATTAAAAAGAAAAAT

Downstream 100 bases:

>100_bases
CGCAGAAATTAGCAAGGCAGAAGCTTAACCGTACCACGCCCGACAGCGCTACGTTTGTCGGGCCAACCACCTCCCGGTAT
TAACGCGACGCCAGACCAAA

Product: disulfide isomerase/thiol-disulfide oxidase

Products: protein with oxidized disulfide bonds [C]

Alternate protein names: NA

Number of amino acids: Translated: 248; Mature: 248

Protein sequence:

>248_residues
MIKRTLLLAMLPILAHAEELPALVKAIEKQSITILKSFEAPGGMKGYLGKYQDMGVTIYLTPDGKHAISGYMYNEKGENL
SNALIEKEIYAPAGREMWQKMEKASWILDGKKDAPVVLYVFADPFCPYCKQFWQQARPWVESGKVQLRTLLVGVIKPESP
ATAAAILAAKNPAKTWHDYEASAGKMKLEVPASIPPAQMKVINQNQQLMDDLGANATPAIYYMNKDKILQQVVGLPEKAQ
LDAMMGQP

Sequences:

>Translated_248_residues
MIKRTLLLAMLPILAHAEELPALVKAIEKQSITILKSFEAPGGMKGYLGKYQDMGVTIYLTPDGKHAISGYMYNEKGENL
SNALIEKEIYAPAGREMWQKMEKASWILDGKKDAPVVLYVFADPFCPYCKQFWQQARPWVESGKVQLRTLLVGVIKPESP
ATAAAILAAKNPAKTWHDYEASAGKMKLEVPASIPPAQMKVINQNQQLMDDLGANATPAIYYMNKDKILQQVVGLPEKAQ
LDAMMGQP
>Mature_248_residues
MIKRTLLLAMLPILAHAEELPALVKAIEKQSITILKSFEAPGGMKGYLGKYQDMGVTIYLTPDGKHAISGYMYNEKGENL
SNALIEKEIYAPAGREMWQKMEKASWILDGKKDAPVVLYVFADPFCPYCKQFWQQARPWVESGKVQLRTLLVGVIKPESP
ATAAAILAAKNPAKTWHDYEASAGKMKLEVPASIPPAQMKVINQNQQLMDDLGANATPAIYYMNKDKILQQVVGLPEKAQ
LDAMMGQP

Specific function: Involved in disulfide bond formation. Functions probably as a disulfide isomerase with a narrower substrate specificity than DsbC. DsbG is maintained in a reduced state by DsbD. In vitro, displays chaperone activity in both redox states [H]

COG id: COG1651

COG function: function code O; Protein-disulfide isomerase

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thioredoxin family. DsbC subfamily [H]

Homologues:

Organism=Escherichia coli, GI87081762, Length=231, Percent_Identity=80.5194805194805, Blast_Score=400, Evalue=1e-113,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018950
- InterPro:   IPR012336
- InterPro:   IPR012335 [H]

Pfam domain/function: NA

EC number: NA

Molecular weight: Translated: 27481; Mature: 27481

Theoretical pI: Translated: 8.89; Mature: 8.89

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
5.2 %Met     (Translated Protein)
6.0 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
5.2 %Met     (Mature Protein)
6.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIKRTLLLAMLPILAHAEELPALVKAIEKQSITILKSFEAPGGMKGYLGKYQDMGVTIYL
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEECCCCCCCHHHCCCHHHCCEEEEE
TPDGKHAISGYMYNEKGENLSNALIEKEIYAPAGREMWQKMEKASWILDGKKDAPVVLYV
CCCCCCHHCCEEECCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHEEECCCCCCCEEEEE
FADPFCPYCKQFWQQARPWVESGKVQLRTLLVGVIKPESPATAAAILAAKNPAKTWHDYE
ECCCCCHHHHHHHHHCCCHHHCCCEEEEHHEEHHCCCCCCCHHHHHEECCCCCCCCCCCC
ASAGKMKLEVPASIPPAQMKVINQNQQLMDDLGANATPAIYYMNKDKILQQVVGLPEKAQ
CCCCCEEEEECCCCCHHHHHHHCCCHHHHHHHCCCCCCEEEEECHHHHHHHHHCCCCHHH
LDAMMGQP
HHHHCCCC
>Mature Secondary Structure
MIKRTLLLAMLPILAHAEELPALVKAIEKQSITILKSFEAPGGMKGYLGKYQDMGVTIYL
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEECCCCCCCHHHCCCHHHCCEEEEE
TPDGKHAISGYMYNEKGENLSNALIEKEIYAPAGREMWQKMEKASWILDGKKDAPVVLYV
CCCCCCHHCCEEECCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHEEECCCCCCCEEEEE
FADPFCPYCKQFWQQARPWVESGKVQLRTLLVGVIKPESPATAAAILAAKNPAKTWHDYE
ECCCCCHHHHHHHHHCCCHHHCCCEEEEHHEEHHCCCCCCCHHHHHEECCCCCCCCCCCC
ASAGKMKLEVPASIPPAQMKVINQNQQLMDDLGANATPAIYYMNKDKILQQVVGLPEKAQ
CCCCCEEEEECCCCCHHHHHHHCCCHHHHHHHCCCCCCEEEEECHHHHHHHHHCCCCHHH
LDAMMGQP
HHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: protein with reduced SH groups [C]

Specific reaction: protein with reduced SH groups = protein with oxidized disulfide bonds [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]