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The map label for this gene is citT [H]

Identifier: 29142649

GI number: 29142649

Start: 2313832

End: 2315367

Strand: Direct

Name: citT [H]

Synonym: t2249

Alternate gene names: 29142649

Gene position: 2313832-2315367 (Clockwise)

Preceding gene: 29142648

Following gene: 29142650

Centisome position: 48.29

GC content: 53.65

Gene sequence:

>1536_bases
ATGTCTTTATCCAAAGATAATATATGGAAGTTGTTGGCCCCATTAGTGGTGATGGGCGTCATGTTGCTAATTCCTGTTCC
AGATGGTATGCCGCCGCAGGCCTGGCACTATTTTGCGGTTTTCGTCGCGATGATTGTCGGTATGATCCTGGAGCCGATCC
CGGCGACAGCGATCAGTTTTATCGCCGTAACCATTTGCGTTATCGGCAGCAATTACCTGCTATTTGACGCTTCAGAACTG
GCCGATCCGGCGTTTAAAGCCAGTAAACAGGCGCTGAAATGGGGGCTTGCCGGTTTCTCCAGCACCACGGTCTGGCTGGT
TTTCGGCGCGTTTATTTTCGCGCTGGGCTACGAAGTGACCGGACTGGGGCGCCGCATTGCGCTGTTTCTGGTAAAATTCA
TGGGTAAACGCACGCTGACGCTCGGCTATGCGATTGTCATTATCGATATTTTGCTGGCGCCGTTTACTCCCTCCAATACG
GCTCGTACCGGCGGGACTGTCTTCCCGGTTATCAAGAACCTGCCGCCGCTGTTTAAATCGTTTCCAAACGATCCTTCCGC
ACGCCGCATCGGCGGTTATTTGATGTGGATGATGGTGATCAGTACCAGCCTGAGCTCTTCGATGTTTGTCACTGGCGCGG
CGCCAAACGTGTTAGGTCTGGAGTTCGTCAGCAAGATCGCCGGGGTCCAGATTAGCTGGCTGCAGTGGTTTCTCAGCTTT
CTGCCGGTAGGGATTATTTTACTGATCGTTGCGCCGTGGCTCTCCTATGTGCTTTATAAACCTGAAGTGACCCACAGTGC
GGAAGTCGCGGCCTGGGCTGGCGGCGAGTTAAAAAACATGGGGCGTCTGTCCCGCAAAGAGTGGACGCTGATTGGCCTGG
TGCTGCTTAGCTTAGGATTGTGGGTTTTTGGTGGAAAAATCATCAACGCCACTGCCGTGGGTCTGCTGGCCGTCTCGCTG
ATGCTGGCGCTGCATGTTGTGCCGTGGAAAGATATTACGCGCTACAACAGCGCCTGGAACACCCTGGTGAACCTCGCTAC
GCTGGTGGTCATGGCCAATGGCTTAACCCGCTCCGGCTTTATTGACTGGTTCGCCAATACCATGAGTACGCATCTGGAAG
GTTTCTCGCCGGATGCAACCGTCATTGTACTGGTACTGGTACTGGTACTGGTACTGGTACTGGTACTGGTACTGGTACTG
GTACTGGTACTGGTACTGGTACTGGTACTGGTATTCTATTTCGCGCACTATTTGTTCGCCAGTCTTTCCGCTCACACCGC
GACCATGCTGCCGGTCATTCTGGCGGTGGGTAAAGGCATTCCGGGCGTTCCGATGGAACAACTGTGTATTCTGCTGGTGC
TGTCTATCGGTATCATGGGCTGTCTGACGCCGTATGCCACCGGCCCTGGTGTGATTATCTACGGCTGCGGTTACGTGAAA
TCCAGAGACTACTGGCGGCTTGGCGCAATCTTTGGCGTTATCTATATCGCAATGCTGTTGCTGGTGGGCTGGCCGATTCT
GGCAATGTGGAATTAA

Upstream 100 bases:

>100_bases
GGTAGCGCCGATCTGCTGATCGTGACCTGGTTTTTAGCGCAAATTTCGCAAGTTAATCATTAACACAATTATAGAAAATA
TCCTTTCCGGAGAGTCATTT

Downstream 100 bases:

>100_bases
ACATTACGCCAACGTAGGTTAAAAATGATGCCGTTCAGGTTCACCTGAGCGGCATTTTTTATGATTAAATGAGTAACATT
CATAATGTCTATAAATTATC

Product: citrate carrier

Products: NA

Alternate protein names: Citrate transporter; Citrate/succinate antiporter [H]

Number of amino acids: Translated: 511; Mature: 510

Protein sequence:

>511_residues
MSLSKDNIWKLLAPLVVMGVMLLIPVPDGMPPQAWHYFAVFVAMIVGMILEPIPATAISFIAVTICVIGSNYLLFDASEL
ADPAFKASKQALKWGLAGFSSTTVWLVFGAFIFALGYEVTGLGRRIALFLVKFMGKRTLTLGYAIVIIDILLAPFTPSNT
ARTGGTVFPVIKNLPPLFKSFPNDPSARRIGGYLMWMMVISTSLSSSMFVTGAAPNVLGLEFVSKIAGVQISWLQWFLSF
LPVGIILLIVAPWLSYVLYKPEVTHSAEVAAWAGGELKNMGRLSRKEWTLIGLVLLSLGLWVFGGKIINATAVGLLAVSL
MLALHVVPWKDITRYNSAWNTLVNLATLVVMANGLTRSGFIDWFANTMSTHLEGFSPDATVIVLVLVLVLVLVLVLVLVL
VLVLVLVLVLVFYFAHYLFASLSAHTATMLPVILAVGKGIPGVPMEQLCILLVLSIGIMGCLTPYATGPGVIIYGCGYVK
SRDYWRLGAIFGVIYIAMLLLVGWPILAMWN

Sequences:

>Translated_511_residues
MSLSKDNIWKLLAPLVVMGVMLLIPVPDGMPPQAWHYFAVFVAMIVGMILEPIPATAISFIAVTICVIGSNYLLFDASEL
ADPAFKASKQALKWGLAGFSSTTVWLVFGAFIFALGYEVTGLGRRIALFLVKFMGKRTLTLGYAIVIIDILLAPFTPSNT
ARTGGTVFPVIKNLPPLFKSFPNDPSARRIGGYLMWMMVISTSLSSSMFVTGAAPNVLGLEFVSKIAGVQISWLQWFLSF
LPVGIILLIVAPWLSYVLYKPEVTHSAEVAAWAGGELKNMGRLSRKEWTLIGLVLLSLGLWVFGGKIINATAVGLLAVSL
MLALHVVPWKDITRYNSAWNTLVNLATLVVMANGLTRSGFIDWFANTMSTHLEGFSPDATVIVLVLVLVLVLVLVLVLVL
VLVLVLVLVLVFYFAHYLFASLSAHTATMLPVILAVGKGIPGVPMEQLCILLVLSIGIMGCLTPYATGPGVIIYGCGYVK
SRDYWRLGAIFGVIYIAMLLLVGWPILAMWN
>Mature_510_residues
SLSKDNIWKLLAPLVVMGVMLLIPVPDGMPPQAWHYFAVFVAMIVGMILEPIPATAISFIAVTICVIGSNYLLFDASELA
DPAFKASKQALKWGLAGFSSTTVWLVFGAFIFALGYEVTGLGRRIALFLVKFMGKRTLTLGYAIVIIDILLAPFTPSNTA
RTGGTVFPVIKNLPPLFKSFPNDPSARRIGGYLMWMMVISTSLSSSMFVTGAAPNVLGLEFVSKIAGVQISWLQWFLSFL
PVGIILLIVAPWLSYVLYKPEVTHSAEVAAWAGGELKNMGRLSRKEWTLIGLVLLSLGLWVFGGKIINATAVGLLAVSLM
LALHVVPWKDITRYNSAWNTLVNLATLVVMANGLTRSGFIDWFANTMSTHLEGFSPDATVIVLVLVLVLVLVLVLVLVLV
LVLVLVLVLVFYFAHYLFASLSAHTATMLPVILAVGKGIPGVPMEQLCILLVLSIGIMGCLTPYATGPGVIIYGCGYVKS
RDYWRLGAIFGVIYIAMLLLVGWPILAMWN

Specific function: Responsible for the uptake of citrate in exchange to the efflux of succinate. Has a relatively broad specificity for C(4)- dicarboxylates and tricarboxylates [H]

COG id: COG0471

COG function: function code P; Di- and tricarboxylate transporters

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the SLC13A transporter (TC 2.A.47) family. SODIT1 subfamily [H]

Homologues:

Organism=Escherichia coli, GI1786829, Length=511, Percent_Identity=90.4109589041096, Blast_Score=917, Evalue=0.0,
Organism=Escherichia coli, GI1789444, Length=495, Percent_Identity=43.2323232323232, Blast_Score=427, Evalue=1e-121,
Organism=Escherichia coli, GI1786986, Length=515, Percent_Identity=32.0388349514563, Blast_Score=251, Evalue=8e-68,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001898 [H]

Pfam domain/function: PF00939 Na_sulph_symp [H]

EC number: NA

Molecular weight: Translated: 55488; Mature: 55357

Theoretical pI: Translated: 9.57; Mature: 9.57

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLSKDNIWKLLAPLVVMGVMLLIPVPDGMPPQAWHYFAVFVAMIVGMILEPIPATAISF
CCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHH
IAVTICVIGSNYLLFDASELADPAFKASKQALKWGLAGFSSTTVWLVFGAFIFALGYEVT
HHHHHHHHCCCEEEEEHHHHCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHH
GLGRRIALFLVKFMGKRTLTLGYAIVIIDILLAPFTPSNTARTGGTVFPVIKNLPPLFKS
HHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHCCCHHHHC
FPNDPSARRIGGYLMWMMVISTSLSSSMFVTGAAPNVLGLEFVSKIAGVQISWLQWFLSF
CCCCCCHHHHHHHHHHHHHHHHHCCCCEEEECCCCCHHHHHHHHHHHCCCHHHHHHHHHH
LPVGIILLIVAPWLSYVLYKPEVTHSAEVAAWAGGELKNMGRLSRKEWTLIGLVLLSLGL
HHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
WVFGGKIINATAVGLLAVSLMLALHVVPWKDITRYNSAWNTLVNLATLVVMANGLTRSGF
HHHCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHH
IDWFANTMSTHLEGFSPDATVIVLVLVLVLVLVLVLVLVLVLVLVLVLVLVFYFAHYLFA
HHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SLSAHTATMLPVILAVGKGIPGVPMEQLCILLVLSIGIMGCLTPYATGPGVIIYGCGYVK
HHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCC
SRDYWRLGAIFGVIYIAMLLLVGWPILAMWN
CCCHHHHHHHHHHHHHHHHHHHCCHHHHCCC
>Mature Secondary Structure 
SLSKDNIWKLLAPLVVMGVMLLIPVPDGMPPQAWHYFAVFVAMIVGMILEPIPATAISF
CCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHH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CCCHHHHHHHHHHHHHHHHHHHCCHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]