Click here to switch to the map view.

The map label for this gene is rna [H]

Identifier: 29142650

GI number: 29142650

Start: 2315477

End: 2316283

Strand: Direct

Name: rna [H]

Synonym: t2250

Alternate gene names: 29142650

Gene position: 2315477-2316283 (Clockwise)

Preceding gene: 29142649

Following gene: 29142651

Centisome position: 48.32

GC content: 55.14

Gene sequence:

>807_bases
ATGAACACAATATGGCATTACAGTCCGCTGCTGGCTGTCCTCTTAACCCCGATTTTTGCCGCCAACGCCGACGAACTTCA
GGCGCAACAGTACGGTGATTTCACTGACTATGTGCTGGCGCTTTCCTGGCAAACCGGTTTTTGTCAAAGCCAGCATGAAC
GCCGCCATCGGGAACCCGATGAGTGCCGTCTGCAAAAAGAGCCAGCCAACAAAGCAGACTTTCTGACCGTCCACGGCCTG
TGGCCAGGATTACCCAAATCTATCGCCGCACGCGGTGTTGACCAGAGACGCTGGCAGCGCTTTGGTTGTGCGACGCGGCC
AATCCCGAATCTGCCTGAAGTCAAGGCCAGCCGGAAATGCTCCGCCCCCGCCCCCGGACTTTCGCCTGACATCGCGACAG
CGCTTAAGGAAGTGATGCCCGGCGCGGGGGGGAATTCCTGTCTGGAGCGCTACGAATATGCCAAACATGGCGCCTGTTTC
GGGTTTGATCCCAATGCCTATTTCGGCACCATGATACGGCTGGATAAGGCGATAAAAGACAGCGCGCTGGGGCAGTTTCT
GGCGGATAATTACGGCAAGACCGTTAGCCGCGCTGAGTTTGATAGCGTCGTCGCACAAACGTGGGGCCAGGATAATGTCA
AAGCCGTTAAAGTAAACTGCCATGGCAATCCCGCCTATTTAACAGAGATTCAGTTTTCATTAAAGGCGTCGATGATTAAC
GCCCCGCTCTCCTCTGCCTCATTCCAGCCGCAGCCGCATCCCGGCAACTGCGGGAAACAGTTTATTATCGATAAAGCCGG
TTATTGA

Upstream 100 bases:

>100_bases
CAACGTAGGTTAAAAATGATGCCGTTCAGGTTCACCTGAGCGGCATTTTTTATGATTAAATGAGTAACATTCATAATGTC
TATAAATTATCGAATTCATC

Downstream 100 bases:

>100_bases
CGAAGCAGAGGTTTAAAACCGTAGCCACATTTGAGAACACGCCCGAATGTAGACTAAAATTCACTCAGGGCTATGACGTT
TGAGGATTTACCCGAATGTG

Product: ribonuclease I

Products: NA

Alternate protein names: RNase I; Enterobacter ribonuclease [H]

Number of amino acids: Translated: 268; Mature: 268

Protein sequence:

>268_residues
MNTIWHYSPLLAVLLTPIFAANADELQAQQYGDFTDYVLALSWQTGFCQSQHERRHREPDECRLQKEPANKADFLTVHGL
WPGLPKSIAARGVDQRRWQRFGCATRPIPNLPEVKASRKCSAPAPGLSPDIATALKEVMPGAGGNSCLERYEYAKHGACF
GFDPNAYFGTMIRLDKAIKDSALGQFLADNYGKTVSRAEFDSVVAQTWGQDNVKAVKVNCHGNPAYLTEIQFSLKASMIN
APLSSASFQPQPHPGNCGKQFIIDKAGY

Sequences:

>Translated_268_residues
MNTIWHYSPLLAVLLTPIFAANADELQAQQYGDFTDYVLALSWQTGFCQSQHERRHREPDECRLQKEPANKADFLTVHGL
WPGLPKSIAARGVDQRRWQRFGCATRPIPNLPEVKASRKCSAPAPGLSPDIATALKEVMPGAGGNSCLERYEYAKHGACF
GFDPNAYFGTMIRLDKAIKDSALGQFLADNYGKTVSRAEFDSVVAQTWGQDNVKAVKVNCHGNPAYLTEIQFSLKASMIN
APLSSASFQPQPHPGNCGKQFIIDKAGY
>Mature_268_residues
MNTIWHYSPLLAVLLTPIFAANADELQAQQYGDFTDYVLALSWQTGFCQSQHERRHREPDECRLQKEPANKADFLTVHGL
WPGLPKSIAARGVDQRRWQRFGCATRPIPNLPEVKASRKCSAPAPGLSPDIATALKEVMPGAGGNSCLERYEYAKHGACF
GFDPNAYFGTMIRLDKAIKDSALGQFLADNYGKTVSRAEFDSVVAQTWGQDNVKAVKVNCHGNPAYLTEIQFSLKASMIN
APLSSASFQPQPHPGNCGKQFIIDKAGY

Specific function: One of the few RNases that cleaves the phosphodiester bond between any two nucleotide. Shows a preference for cytidylic or guanylic acid [H]

COG id: COG3719

COG function: function code J; Ribonuclease I

Gene ontology:

Cell location: Periplasm. Cytoplasm. Note=RNase I (periplasmic) and RNase I* (cytoplasmic) appear to be isoforms apparently encoded by the same gene. The cytoplasmic form is less active towards natural polymer RNA [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNase T2 family [H]

Homologues:

Organism=Escherichia coli, GI1786828, Length=268, Percent_Identity=73.5074626865672, Blast_Score=427, Evalue=1e-121,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001568
- InterPro:   IPR018188 [H]

Pfam domain/function: PF00445 Ribonuclease_T2 [H]

EC number: =3.1.27.6 [H]

Molecular weight: Translated: 29551; Mature: 29551

Theoretical pI: Translated: 8.21; Mature: 8.21

Prosite motif: PS00530 RNASE_T2_1 ; PS00531 RNASE_T2_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.0 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
3.0 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNTIWHYSPLLAVLLTPIFAANADELQAQQYGDFTDYVLALSWQTGFCQSQHERRHREPD
CCCCHHHHHHHHHHHHHHHHCCHHHHHHHHCCCHHHHHEEEECCCCCHHHHHHHHCCCCH
ECRLQKEPANKADFLTVHGLWPGLPKSIAARGVDQRRWQRFGCATRPIPNLPEVKASRKC
HHHCCCCCCCCCCEEEEECCCCCCCHHHHHCCCHHHHHHHHCCCCCCCCCCCCCCCCCCC
SAPAPGLSPDIATALKEVMPGAGGNSCLERYEYAKHGACFGFDPNAYFGTMIRLDKAIKD
CCCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCEECCCCCHHHHHHHHHHHHHHH
SALGQFLADNYGKTVSRAEFDSVVAQTWGQDNVKAVKVNCHGNPAYLTEIQFSLKASMIN
HHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCEEEEHHHHHHHHHHHH
APLSSASFQPQPHPGNCGKQFIIDKAGY
CCCCCCCCCCCCCCCCCCCEEEEECCCC
>Mature Secondary Structure
MNTIWHYSPLLAVLLTPIFAANADELQAQQYGDFTDYVLALSWQTGFCQSQHERRHREPD
CCCCHHHHHHHHHHHHHHHHCCHHHHHHHHCCCHHHHHEEEECCCCCHHHHHHHHCCCCH
ECRLQKEPANKADFLTVHGLWPGLPKSIAARGVDQRRWQRFGCATRPIPNLPEVKASRKC
HHHCCCCCCCCCCEEEEECCCCCCCHHHHHCCCHHHHHHHHCCCCCCCCCCCCCCCCCCC
SAPAPGLSPDIATALKEVMPGAGGNSCLERYEYAKHGACFGFDPNAYFGTMIRLDKAIKD
CCCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCEECCCCCHHHHHHHHHHHHHHH
SALGQFLADNYGKTVSRAEFDSVVAQTWGQDNVKAVKVNCHGNPAYLTEIQFSLKASMIN
HHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCEEEEHHHHHHHHHHHH
APLSSASFQPQPHPGNCGKQFIIDKAGY
CCCCCCCCCCCCCCCCCCCEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2253883; 8905232; 9278503; 8188249 [H]