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The map label for this gene is leuS [H]

Identifier: 29142620

GI number: 29142620

Start: 2284615

End: 2287197

Strand: Direct

Name: leuS [H]

Synonym: t2219

Alternate gene names: 29142620

Gene position: 2284615-2287197 (Clockwise)

Preceding gene: 29142614

Following gene: 29142621

Centisome position: 47.68

GC content: 55.83

Gene sequence:

>2583_bases
ATGCAAGAGCAATACCGCCCGGAAGAGATAGAATCCAAAGTACAGCTTCACTGGGATGAGAAGCGCACATTTGAAGTGAC
CGAAGACGAGAGCAAAGAGAAGTATTACTGCCTGTCGATGCTTCCCTATCCTTCTGGCCGACTACACATGGGCCACGTAC
GTAACTACACCATCGGCGACGTGGTTGCCCGCTACCAGCATATGCTGGGCAAAAACGTGCTGCAGCCAATCGGCTGGGAC
GCCTTCGGCCTGCCGGCGGAAGGCGCGGCGGTGAAGAACAACACCGCGCCGGCGCCGTGGACGTACGACAACATCGCGTA
CATGAAAAACCAGCTCAAAACGCTGGGTTTTGGCTATGACTGGAGCCGTGAAATCGCGACCTGCACCCCGGAATACTACC
GTTGGGAACAGAAATTCTTCACCGAGCTGTATAAAAAAGGCCTGGTGTACAAGAAGACTTCTGCCGTTAACTGGTGCCCG
AACGATCAGACCGTTCTCGCCAACGAACAGGTGATCGACGGCTGCTGCTGGCGTTGCGACACCAAAGTTGAGCGTAAAGA
GATCCCACAGTGGTTTATCAAAATCACCGCTTACGCAGACGAGCTGTTGCGCGATCTGGATAAGCTGGACCACTGGCCTG
ATACCGTAAAAACGATGCAGCGCAACTGGATTGGCCGCTCTGAAGGCGTGGAAATCACCTTCGATGTGAAGGGTTACGAT
AACACGCTGACCGTCTATACCACCCGTCCGGATACCTTTATGGGCGCCACCTACCTGGCAGTGGCCGCCGGTCATCCGCT
GGCGCAAAAAGCGGCGGCGAACAACGCCGAGCTGGCGGCGTTCATCGACGAATGCCGTAACACCAAAGTGGCCGAAGCCG
AAATGGCGACCATGGAGAAAAAAGGCGTCGACACCGGCTATAAAGCGATTCATCCGTTAACTGGCGAAGAGATCCCGGTC
TGGGCGGCAAACTTTGTCCTGATGGAGTACGGCACTGGCGCGGTGATGGCCGTTCCGGGTCACGATCAGCGCGACTACGA
ATTTGCCTCCAAATACGGTTTGACCATCAAGCCGGTGATTCTGGCCGCTGACGGTTCCGAGCCGGATCTTTCCGAACAGG
CGCTGACCGAAAAAGGCGTGCTGTTTAACTCCGGTGAGTTCGACGGTCTGGCCTTTGAAGCGGCGTTTAACGCCATTGCC
GATAAACTGGCGGAAAAAGGCGTCGGCGAACGTAAAGTGAACTACCGTCTGCGTGACTGGGGCGTTTCCCGTCAGCGTTA
CTGGGGCGCGCCGATCCCGATGGTCACCCTGGAAGACGGCACCGTGCTGCCGACGCCGGAAGATCAACTGCCGGTTATTT
TGCCGGAAGACGTCGTCATGGACGGCATCACCAGCCCGATCAAGGCCGATCCTGAGTGGGCGAAAACCACCGTTAACGGT
ATGCCGGCGCTGCGTGAAACCGACACCTTCGACACCTTTATGGAATCGTCATGGTACTACGCGCGTTACACCTGCCCGCA
GTATCAGGAAGGAATGCTGGATTCGAAAGCGGCAAACTACTGGCTGCCAGTCGACATCTACATCGGCGGTATTGAACATG
CCATCATGCACCTGCTCTACTTCCGCTTCTTCCATAAGCTGATGCGCGATGCGGGCATGGTCACCTCTGACGAGCCGGCT
AAACAGTTGCTGTGCCAGGGCATGGTACTGGCGGATGCGTTCTATTATGTCGGCGAGAACGGCGAGCGTAACTGGGTTTC
GCCAGTTGACGCTATCGTCGAGCGCGATGAAAAAGGCCGCATCGTCAAAGCAAAAGACGCAGCGGGTCATGAGCTGGTCT
ACACTGGCATGAGTAAAATGTCCAAGTCGAAAAATAACGGTATCGACCCGCAGGTAATGGTTGAACGTTATGGCGCGGAT
ACGGTACGCCTGTTTATGATGTTCGCTTCTCCGGCGGATATGACTCTGGAATGGCAGGAGTCCGGCGTAGAAGGCGCAAA
CCGCTTTATTAAACGCGTCTGGAAGCTGGTTTACGAACACACCGCTAAAGGTCCTGTCGCGGCGCTGAACGTCGATGCGC
TGAGCGAAGATCAAAAAGCGCTGCGTCGCGATGTGCATAAAACCATTGCCAAAGTGACCGATGATATCGGCCGCCGTCAG
ACGTTCAACACCGCCATTGCGGCGATCATGGAGTTGATGAACAAACTGGCGAAAGCCCCGCAGGAAGGCGAACAAGACCG
CGCACTGCTTCAGGAAGCGCTGCAGGCCGTCGTGCGTATGCTTAACCCGTTCACCCCGCACGTCTGCTTTACCTTGTGGC
AGGAACTGGGTGGCGAAGGCGATATCGACAACGCGCCGTGGCCGGTTGCCGACGAGCAGGCAATGGTGGAAAACACCACG
CTGGTTGTCGTGCAGGTTAACGGTAAAGTTCGCGGTAAGATCACCGTTGCGGTGGATGCCACCGAAGAACAGGTGCGTGA
ACGTGCAGGCCAGGAACATCTGGTGGCAAAATATCTTGATGGCGTTACCGTGCGTAAAGTGATTTACGTTCCGGGTAAAC
TCCTCAATCTGGTTGTTGGCTAA

Upstream 100 bases:

>100_bases
CCCTGTTTTGACGTGCGTGTTTGGGCTATGCTATGCGGATCTAAACACCACCTCTAAAGCTACATTTGTAGCTGTATCGA
AAACAGGACTACTGGCTGCC

Downstream 100 bases:

>100_bases
GCGCGGGAGGAAGCGTGCGATATCTGGTAACACTGTTGTTATCTCTGGCGGTGCTGGTCACCGCCGGATGCGGCTGGCAT
CTGCGTAGCACTACGCAGGT

Product: leucyl-tRNA synthetase

Products: NA

Alternate protein names: Leucine--tRNA ligase; LeuRS [H]

Number of amino acids: Translated: 860; Mature: 860

Protein sequence:

>860_residues
MQEQYRPEEIESKVQLHWDEKRTFEVTEDESKEKYYCLSMLPYPSGRLHMGHVRNYTIGDVVARYQHMLGKNVLQPIGWD
AFGLPAEGAAVKNNTAPAPWTYDNIAYMKNQLKTLGFGYDWSREIATCTPEYYRWEQKFFTELYKKGLVYKKTSAVNWCP
NDQTVLANEQVIDGCCWRCDTKVERKEIPQWFIKITAYADELLRDLDKLDHWPDTVKTMQRNWIGRSEGVEITFDVKGYD
NTLTVYTTRPDTFMGATYLAVAAGHPLAQKAAANNAELAAFIDECRNTKVAEAEMATMEKKGVDTGYKAIHPLTGEEIPV
WAANFVLMEYGTGAVMAVPGHDQRDYEFASKYGLTIKPVILAADGSEPDLSEQALTEKGVLFNSGEFDGLAFEAAFNAIA
DKLAEKGVGERKVNYRLRDWGVSRQRYWGAPIPMVTLEDGTVLPTPEDQLPVILPEDVVMDGITSPIKADPEWAKTTVNG
MPALRETDTFDTFMESSWYYARYTCPQYQEGMLDSKAANYWLPVDIYIGGIEHAIMHLLYFRFFHKLMRDAGMVTSDEPA
KQLLCQGMVLADAFYYVGENGERNWVSPVDAIVERDEKGRIVKAKDAAGHELVYTGMSKMSKSKNNGIDPQVMVERYGAD
TVRLFMMFASPADMTLEWQESGVEGANRFIKRVWKLVYEHTAKGPVAALNVDALSEDQKALRRDVHKTIAKVTDDIGRRQ
TFNTAIAAIMELMNKLAKAPQEGEQDRALLQEALQAVVRMLNPFTPHVCFTLWQELGGEGDIDNAPWPVADEQAMVENTT
LVVVQVNGKVRGKITVAVDATEEQVRERAGQEHLVAKYLDGVTVRKVIYVPGKLLNLVVG

Sequences:

>Translated_860_residues
MQEQYRPEEIESKVQLHWDEKRTFEVTEDESKEKYYCLSMLPYPSGRLHMGHVRNYTIGDVVARYQHMLGKNVLQPIGWD
AFGLPAEGAAVKNNTAPAPWTYDNIAYMKNQLKTLGFGYDWSREIATCTPEYYRWEQKFFTELYKKGLVYKKTSAVNWCP
NDQTVLANEQVIDGCCWRCDTKVERKEIPQWFIKITAYADELLRDLDKLDHWPDTVKTMQRNWIGRSEGVEITFDVKGYD
NTLTVYTTRPDTFMGATYLAVAAGHPLAQKAAANNAELAAFIDECRNTKVAEAEMATMEKKGVDTGYKAIHPLTGEEIPV
WAANFVLMEYGTGAVMAVPGHDQRDYEFASKYGLTIKPVILAADGSEPDLSEQALTEKGVLFNSGEFDGLAFEAAFNAIA
DKLAEKGVGERKVNYRLRDWGVSRQRYWGAPIPMVTLEDGTVLPTPEDQLPVILPEDVVMDGITSPIKADPEWAKTTVNG
MPALRETDTFDTFMESSWYYARYTCPQYQEGMLDSKAANYWLPVDIYIGGIEHAIMHLLYFRFFHKLMRDAGMVTSDEPA
KQLLCQGMVLADAFYYVGENGERNWVSPVDAIVERDEKGRIVKAKDAAGHELVYTGMSKMSKSKNNGIDPQVMVERYGAD
TVRLFMMFASPADMTLEWQESGVEGANRFIKRVWKLVYEHTAKGPVAALNVDALSEDQKALRRDVHKTIAKVTDDIGRRQ
TFNTAIAAIMELMNKLAKAPQEGEQDRALLQEALQAVVRMLNPFTPHVCFTLWQELGGEGDIDNAPWPVADEQAMVENTT
LVVVQVNGKVRGKITVAVDATEEQVRERAGQEHLVAKYLDGVTVRKVIYVPGKLLNLVVG
>Mature_860_residues
MQEQYRPEEIESKVQLHWDEKRTFEVTEDESKEKYYCLSMLPYPSGRLHMGHVRNYTIGDVVARYQHMLGKNVLQPIGWD
AFGLPAEGAAVKNNTAPAPWTYDNIAYMKNQLKTLGFGYDWSREIATCTPEYYRWEQKFFTELYKKGLVYKKTSAVNWCP
NDQTVLANEQVIDGCCWRCDTKVERKEIPQWFIKITAYADELLRDLDKLDHWPDTVKTMQRNWIGRSEGVEITFDVKGYD
NTLTVYTTRPDTFMGATYLAVAAGHPLAQKAAANNAELAAFIDECRNTKVAEAEMATMEKKGVDTGYKAIHPLTGEEIPV
WAANFVLMEYGTGAVMAVPGHDQRDYEFASKYGLTIKPVILAADGSEPDLSEQALTEKGVLFNSGEFDGLAFEAAFNAIA
DKLAEKGVGERKVNYRLRDWGVSRQRYWGAPIPMVTLEDGTVLPTPEDQLPVILPEDVVMDGITSPIKADPEWAKTTVNG
MPALRETDTFDTFMESSWYYARYTCPQYQEGMLDSKAANYWLPVDIYIGGIEHAIMHLLYFRFFHKLMRDAGMVTSDEPA
KQLLCQGMVLADAFYYVGENGERNWVSPVDAIVERDEKGRIVKAKDAAGHELVYTGMSKMSKSKNNGIDPQVMVERYGAD
TVRLFMMFASPADMTLEWQESGVEGANRFIKRVWKLVYEHTAKGPVAALNVDALSEDQKALRRDVHKTIAKVTDDIGRRQ
TFNTAIAAIMELMNKLAKAPQEGEQDRALLQEALQAVVRMLNPFTPHVCFTLWQELGGEGDIDNAPWPVADEQAMVENTT
LVVVQVNGKVRGKITVAVDATEEQVRERAGQEHLVAKYLDGVTVRKVIYVPGKLLNLVVG

Specific function: Unknown

COG id: COG0495

COG function: function code J; Leucyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI7661872, Length=863, Percent_Identity=36.7323290845886, Blast_Score=514, Evalue=1e-145,
Organism=Escherichia coli, GI1786861, Length=860, Percent_Identity=95.2325581395349, Blast_Score=1717, Evalue=0.0,
Organism=Escherichia coli, GI1790708, Length=393, Percent_Identity=26.2086513994911, Blast_Score=113, Evalue=5e-26,
Organism=Caenorhabditis elegans, GI71997510, Length=881, Percent_Identity=29.6254256526674, Blast_Score=350, Evalue=1e-96,
Organism=Caenorhabditis elegans, GI71997517, Length=873, Percent_Identity=29.6678121420389, Blast_Score=350, Evalue=2e-96,
Organism=Caenorhabditis elegans, GI212645227, Length=437, Percent_Identity=26.3157894736842, Blast_Score=133, Evalue=5e-31,
Organism=Caenorhabditis elegans, GI71980946, Length=151, Percent_Identity=29.1390728476821, Blast_Score=67, Evalue=5e-11,
Organism=Saccharomyces cerevisiae, GI6323414, Length=809, Percent_Identity=36.9592088998764, Blast_Score=504, Evalue=1e-143,
Organism=Saccharomyces cerevisiae, GI6321531, Length=387, Percent_Identity=26.6149870801034, Blast_Score=100, Evalue=8e-22,
Organism=Saccharomyces cerevisiae, GI6325217, Length=357, Percent_Identity=23.5294117647059, Blast_Score=73, Evalue=2e-13,
Organism=Drosophila melanogaster, GI21355409, Length=882, Percent_Identity=32.8798185941043, Blast_Score=440, Evalue=1e-123,
Organism=Drosophila melanogaster, GI281366294, Length=143, Percent_Identity=31.4685314685315, Blast_Score=74, Evalue=4e-13,

Paralogues:

None

Copy number: 800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001412
- InterPro:   IPR002300
- InterPro:   IPR002302
- InterPro:   IPR014729
- InterPro:   IPR009080
- InterPro:   IPR013155
- InterPro:   IPR009008 [H]

Pfam domain/function: PF08264 Anticodon_1; PF00133 tRNA-synt_1 [H]

EC number: =6.1.1.4 [H]

Molecular weight: Translated: 96941; Mature: 96941

Theoretical pI: Translated: 4.97; Mature: 4.97

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQEQYRPEEIESKVQLHWDEKRTFEVTEDESKEKYYCLSMLPYPSGRLHMGHVRNYTIGD
CCCCCCCHHHHCCEEEEECCCCEEEECCCCCCCCEEEEEECCCCCCCEEECCCCCCCHHH
VVARYQHMLGKNVLQPIGWDAFGLPAEGAAVKNNTAPAPWTYDNIAYMKNQLKTLGFGYD
HHHHHHHHHHHHHHHHCCCCCCCCCCCCCEECCCCCCCCCCCCHHHHHHHHHHHHCCCCC
WSREIATCTPEYYRWEQKFFTELYKKGLVYKKTSAVNWCPNDQTVLANEQVIDGCCWRCD
CCCCHHHCCHHHHHHHHHHHHHHHHCCCEEEECCCCEECCCCCEEEECHHHHHHHHHCCC
TKVERKEIPQWFIKITAYADELLRDLDKLDHWPDTVKTMQRNWIGRSEGVEITFDVKGYD
CCHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCEEEEEEECCCC
NTLTVYTTRPDTFMGATYLAVAAGHPLAQKAAANNAELAAFIDECRNTKVAEAEMATMEK
CEEEEEEECCCCHHHHHHHHHHCCCCHHHHHCCCCCHHHHHHHHHCCCCCHHHHHHHHHH
KGVDTGYKAIHPLTGEEIPVWAANFVLMEYGTGAVMAVPGHDQRDYEFASKYGLTIKPVI
CCCCCCHHHCCCCCCCCCCEEEEEEEEEEECCCEEEEECCCCCCCHHHHHHCCCEEEEEE
LAADGSEPDLSEQALTEKGVLFNSGEFDGLAFEAAFNAIADKLAEKGVGERKVNYRLRDW
EEECCCCCCCHHHHHHHCCCEEECCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCEEEHHC
GVSRQRYWGAPIPMVTLEDGTVLPTPEDQLPVILPEDVVMDGITSPIKADPEWAKTTVNG
CCCCHHCCCCCCCEEEECCCEECCCCCCCCCEECCHHHHHHCCCCCCCCCCHHHHHHHCC
MPALRETDTFDTFMESSWYYARYTCPQYQEGMLDSKAANYWLPVDIYIGGIEHAIMHLLY
CCCCCCCCHHHHHHHCCCEEEEECCCHHHHCCCCCCCCCEEEEEEEEECCHHHHHHHHHH
FRFFHKLMRDAGMVTSDEPAKQLLCQGMVLADAFYYVGENGERNWVSPVDAIVERDEKGR
HHHHHHHHHHCCCCCCCCHHHHHHHCCHHHHHHHHEECCCCCCCCCCHHHHHHCCCCCCC
IVKAKDAAGHELVYTGMSKMSKSKNNGIDPQVMVERYGADTVRLFMMFASPADMTLEWQE
EEEECCCCCCEEEEHHHHHHHHCCCCCCCHHHHHHHCCCHHEEEEHHHCCCCCCEEEEHH
SGVEGANRFIKRVWKLVYEHTAKGPVAALNVDALSEDQKALRRDVHKTIAKVTDDIGRRQ
CCCHHHHHHHHHHHHHHHHHCCCCCEEEEEHHHCCHHHHHHHHHHHHHHHHHHHHHHHHH
TFNTAIAAIMELMNKLAKAPQEGEQDRALLQEALQAVVRMLNPFTPHVCFTLWQELGGEG
HHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCC
DIDNAPWPVADEQAMVENTTLVVVQVNGKVRGKITVAVDATEEQVRERAGQEHLVAKYLD
CCCCCCCCCCCCCCEECCCEEEEEEECCEEEEEEEEEEECCHHHHHHHCCHHHHHHHHHC
GVTVRKVIYVPGKLLNLVVG
CCEEEEEEECCHHHHHHHCC
>Mature Secondary Structure
MQEQYRPEEIESKVQLHWDEKRTFEVTEDESKEKYYCLSMLPYPSGRLHMGHVRNYTIGD
CCCCCCCHHHHCCEEEEECCCCEEEECCCCCCCCEEEEEECCCCCCCEEECCCCCCCHHH
VVARYQHMLGKNVLQPIGWDAFGLPAEGAAVKNNTAPAPWTYDNIAYMKNQLKTLGFGYD
HHHHHHHHHHHHHHHHCCCCCCCCCCCCCEECCCCCCCCCCCCHHHHHHHHHHHHCCCCC
WSREIATCTPEYYRWEQKFFTELYKKGLVYKKTSAVNWCPNDQTVLANEQVIDGCCWRCD
CCCCHHHCCHHHHHHHHHHHHHHHHCCCEEEECCCCEECCCCCEEEECHHHHHHHHHCCC
TKVERKEIPQWFIKITAYADELLRDLDKLDHWPDTVKTMQRNWIGRSEGVEITFDVKGYD
CCHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCEEEEEEECCCC
NTLTVYTTRPDTFMGATYLAVAAGHPLAQKAAANNAELAAFIDECRNTKVAEAEMATMEK
CEEEEEEECCCCHHHHHHHHHHCCCCHHHHHCCCCCHHHHHHHHHCCCCCHHHHHHHHHH
KGVDTGYKAIHPLTGEEIPVWAANFVLMEYGTGAVMAVPGHDQRDYEFASKYGLTIKPVI
CCCCCCHHHCCCCCCCCCCEEEEEEEEEEECCCEEEEECCCCCCCHHHHHHCCCEEEEEE
LAADGSEPDLSEQALTEKGVLFNSGEFDGLAFEAAFNAIADKLAEKGVGERKVNYRLRDW
EEECCCCCCCHHHHHHHCCCEEECCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCEEEHHC
GVSRQRYWGAPIPMVTLEDGTVLPTPEDQLPVILPEDVVMDGITSPIKADPEWAKTTVNG
CCCCHHCCCCCCCEEEECCCEECCCCCCCCCEECCHHHHHHCCCCCCCCCCHHHHHHHCC
MPALRETDTFDTFMESSWYYARYTCPQYQEGMLDSKAANYWLPVDIYIGGIEHAIMHLLY
CCCCCCCCHHHHHHHCCCEEEEECCCHHHHCCCCCCCCCEEEEEEEEECCHHHHHHHHHH
FRFFHKLMRDAGMVTSDEPAKQLLCQGMVLADAFYYVGENGERNWVSPVDAIVERDEKGR
HHHHHHHHHHCCCCCCCCHHHHHHHCCHHHHHHHHEECCCCCCCCCCHHHHHHCCCCCCC
IVKAKDAAGHELVYTGMSKMSKSKNNGIDPQVMVERYGADTVRLFMMFASPADMTLEWQE
EEEECCCCCCEEEEHHHHHHHHCCCCCCCHHHHHHHCCCHHEEEEHHHCCCCCCEEEEHH
SGVEGANRFIKRVWKLVYEHTAKGPVAALNVDALSEDQKALRRDVHKTIAKVTDDIGRRQ
CCCHHHHHHHHHHHHHHHHHCCCCCEEEEEHHHCCHHHHHHHHHHHHHHHHHHHHHHHHH
TFNTAIAAIMELMNKLAKAPQEGEQDRALLQEALQAVVRMLNPFTPHVCFTLWQELGGEG
HHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCC
DIDNAPWPVADEQAMVENTTLVVVQVNGKVRGKITVAVDATEEQVRERAGQEHLVAKYLD
CCCCCCCCCCCCCCEECCCEEEEEEECCEEEEEEEEEEECCHHHHHHHCCHHHHHHHHHC
GVTVRKVIYVPGKLLNLVVG
CCEEEEEEECCHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA