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The map label for this gene is rihA [H]

Identifier: 29142614

GI number: 29142614

Start: 2277897

End: 2278832

Strand: Direct

Name: rihA [H]

Synonym: t2212

Alternate gene names: 29142614

Gene position: 2277897-2278832 (Clockwise)

Preceding gene: 29142613

Following gene: 29142620

Centisome position: 47.54

GC content: 57.37

Gene sequence:

>936_bases
ATGGCACTGCCCATTATTATTGATTGCGATCCGGGTCATGATGACGCGATCGCCCTCGTTCTCGCCCTCGCCTCCCCTGA
ACTGGAAGTGAAAGCGATCACCTCTTCCGCCGGCAACCAGACGCCGGAGAAGACGCTGCGCAACGTGCTGCGGATGCTGA
CACTGCTCAAACGCCCGGATATTCCTGTGGCGGGCGGCGCGGTGAAACCGCTCATGCGCGAACTGATTATCGCTGATAAC
GTTCACGGCGAAAGCGGTCTTGACGGCCCGGCGCTGCCCGAGCCGTCATTCGCCCCGCAAAGCGGCACTGCCGTTGAGCT
GATGGCAAAAACACTGCGCGAGAGCGCACAGCCGGTCACCATCGTCTCCACTGGCCCACAGACCAACGTGGCGCTGCTGC
TGAATAGCCATCCGGAACTGCATACGAAAATCGCCCGCATTGTGATCATGGGCGGCGCGATGGCGCTGGGTAACTGGACG
CCCGCTGCGGAATTTAATATTTACGTCGATCCGGAAGCGGCAGAAATTGTCTTCCAGTCAGGCATTCCGGTGGTGATGGC
CGGGCTGGACGTCACCCATAAGGCGCAGATTCACGCCGCCGATATCGAGCGTTTCCGCGACATCGGCAATCCAATTTCGA
CGATCGTGGCTGAACTACTCGACTTCTTCTTTGAATACCACAAAGACGAAAAATGGGGATTTGTCGGCGCGCCGCTGCAC
GACCCTTGCACTATCGCCTGGCTGCTGAAACCTGAGATTTTCACCACCGTTGAGCGTTGGGTTGGCGTGGAAACTCAGGG
GAAATATACCCAGGGGATGACGGTGGTGGATTATTATTTCCTGACGGGGAATAAGCCGAATGCGACGGTGATGGTCGATG
TAGACCGTCAGGGATTTGTCGATTTGCTGGCGGAACGGCTGCAATACTACGCATAA

Upstream 100 bases:

>100_bases
GTCAGAGTGCGCGCTACCTCACGCTTTACTTCTTCGCTTCTCGAAACGTATCCAGCCGGGCGTTAACCTTGTCACAAAGT
ACCGCTTACAAGGAGCCACA

Downstream 100 bases:

>100_bases
AGCGCAAAGAAGAATCCAGGGATGAACAGCGATGTTTTGGCGTAACAATAGACCTGAAATCAGCCTGCTACAGCACGACG
TAGCCCATATAACGTTTTCA

Product: ribonucleoside hydrolase 1

Products: uracil; ribose; cytosine [C]

Alternate protein names: Cytidine/uridine-specific hydrolase [H]

Number of amino acids: Translated: 311; Mature: 310

Protein sequence:

>311_residues
MALPIIIDCDPGHDDAIALVLALASPELEVKAITSSAGNQTPEKTLRNVLRMLTLLKRPDIPVAGGAVKPLMRELIIADN
VHGESGLDGPALPEPSFAPQSGTAVELMAKTLRESAQPVTIVSTGPQTNVALLLNSHPELHTKIARIVIMGGAMALGNWT
PAAEFNIYVDPEAAEIVFQSGIPVVMAGLDVTHKAQIHAADIERFRDIGNPISTIVAELLDFFFEYHKDEKWGFVGAPLH
DPCTIAWLLKPEIFTTVERWVGVETQGKYTQGMTVVDYYFLTGNKPNATVMVDVDRQGFVDLLAERLQYYA

Sequences:

>Translated_311_residues
MALPIIIDCDPGHDDAIALVLALASPELEVKAITSSAGNQTPEKTLRNVLRMLTLLKRPDIPVAGGAVKPLMRELIIADN
VHGESGLDGPALPEPSFAPQSGTAVELMAKTLRESAQPVTIVSTGPQTNVALLLNSHPELHTKIARIVIMGGAMALGNWT
PAAEFNIYVDPEAAEIVFQSGIPVVMAGLDVTHKAQIHAADIERFRDIGNPISTIVAELLDFFFEYHKDEKWGFVGAPLH
DPCTIAWLLKPEIFTTVERWVGVETQGKYTQGMTVVDYYFLTGNKPNATVMVDVDRQGFVDLLAERLQYYA
>Mature_310_residues
ALPIIIDCDPGHDDAIALVLALASPELEVKAITSSAGNQTPEKTLRNVLRMLTLLKRPDIPVAGGAVKPLMRELIIADNV
HGESGLDGPALPEPSFAPQSGTAVELMAKTLRESAQPVTIVSTGPQTNVALLLNSHPELHTKIARIVIMGGAMALGNWTP
AAEFNIYVDPEAAEIVFQSGIPVVMAGLDVTHKAQIHAADIERFRDIGNPISTIVAELLDFFFEYHKDEKWGFVGAPLHD
PCTIAWLLKPEIFTTVERWVGVETQGKYTQGMTVVDYYFLTGNKPNATVMVDVDRQGFVDLLAERLQYYA

Specific function: Hydrolyzes cytidine or uridine to ribose and cytosine or uracil, respectively [H]

COG id: COG1957

COG function: function code F; Inosine-uridine nucleoside N-ribohydrolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the IUNH family. RihA subfamily [H]

Homologues:

Organism=Escherichia coli, GI1786871, Length=311, Percent_Identity=92.2829581993569, Blast_Score=599, Evalue=1e-173,
Organism=Escherichia coli, GI1788486, Length=306, Percent_Identity=39.8692810457516, Blast_Score=229, Evalue=2e-61,
Organism=Escherichia coli, GI1786213, Length=307, Percent_Identity=42.671009771987, Blast_Score=211, Evalue=6e-56,
Organism=Caenorhabditis elegans, GI32565474, Length=189, Percent_Identity=33.3333333333333, Blast_Score=99, Evalue=3e-21,
Organism=Caenorhabditis elegans, GI17565698, Length=188, Percent_Identity=35.1063829787234, Blast_Score=90, Evalue=1e-18,
Organism=Saccharomyces cerevisiae, GI6320608, Length=259, Percent_Identity=36.2934362934363, Blast_Score=145, Evalue=6e-36,
Organism=Drosophila melanogaster, GI24641839, Length=308, Percent_Identity=28.8961038961039, Blast_Score=92, Evalue=3e-19,
Organism=Drosophila melanogaster, GI24583915, Length=326, Percent_Identity=26.3803680981595, Blast_Score=88, Evalue=6e-18,
Organism=Drosophila melanogaster, GI24641837, Length=303, Percent_Identity=30.03300330033, Blast_Score=87, Evalue=2e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015910
- InterPro:   IPR023186
- InterPro:   IPR001910
- InterPro:   IPR022975 [H]

Pfam domain/function: PF01156 IU_nuc_hydro [H]

EC number: NA

Molecular weight: Translated: 33833; Mature: 33702

Theoretical pI: Translated: 4.66; Mature: 4.66

Prosite motif: PS01247 IUNH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MALPIIIDCDPGHDDAIALVLALASPELEVKAITSSAGNQTPEKTLRNVLRMLTLLKRPD
CCCEEEEECCCCCCHHHHHHHHHCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHCCCC
IPVAGGAVKPLMRELIIADNVHGESGLDGPALPEPSFAPQSGTAVELMAKTLRESAQPVT
CCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCEE
IVSTGPQTNVALLLNSHPELHTKIARIVIMGGAMALGNWTPAAEFNIYVDPEAAEIVFQS
EEECCCCCCEEEEECCCCHHHHHHHHHHEECCCEEECCCCCCCEEEEEECCCHHHEEECC
GIPVVMAGLDVTHKAQIHAADIERFRDIGNPISTIVAELLDFFFEYHKDEKWGFVGAPLH
CCCEEEECCCCCCCCEEEHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCEECCCCC
DPCTIAWLLKPEIFTTVERWVGVETQGKYTQGMTVVDYYFLTGNKPNATVMVDVDRQGFV
CCCEEEEEECCHHHHHHHHHCCCCCCCCCCCCCEEEEEEEEECCCCCEEEEEEECCCCHH
DLLAERLQYYA
HHHHHHHHHCC
>Mature Secondary Structure 
ALPIIIDCDPGHDDAIALVLALASPELEVKAITSSAGNQTPEKTLRNVLRMLTLLKRPD
CCEEEEECCCCCCHHHHHHHHHCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHCCCC
IPVAGGAVKPLMRELIIADNVHGESGLDGPALPEPSFAPQSGTAVELMAKTLRESAQPVT
CCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCEE
IVSTGPQTNVALLLNSHPELHTKIARIVIMGGAMALGNWTPAAEFNIYVDPEAAEIVFQS
EEECCCCCCEEEEECCCCHHHHHHHHHHEECCCEEECCCCCCCEEEEEECCCHHHEEECC
GIPVVMAGLDVTHKAQIHAADIERFRDIGNPISTIVAELLDFFFEYHKDEKWGFVGAPLH
CCCEEEECCCCCCCCEEEHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCEECCCCC
DPCTIAWLLKPEIFTTVERWVGVETQGKYTQGMTVVDYYFLTGNKPNATVMVDVDRQGFV
CCCEEEEEECCHHHHHHHHHCCCCCCCCCCCCCEEEEEEEEECCCCCEEEEEEECCCCHH
DLLAERLQYYA
HHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: uridine; H2O; cytidine [C]

Specific reaction: uridine + H2O = uracil + ribose cytidine + H2O = cytosine + ribose [C]

General reaction: Pyrimidine-specific nucleoside hydrolase [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA