Click here to switch to the map view.

The map label for this gene is miaB [H]

Identifier: 29142605

GI number: 29142605

Start: 2268374

End: 2269840

Strand: Direct

Name: miaB [H]

Synonym: t2203

Alternate gene names: 29142605

Gene position: 2268374-2269840 (Clockwise)

Preceding gene: 29142603

Following gene: 29142606

Centisome position: 47.34

GC content: 54.06

Gene sequence:

>1467_bases
ATGCGCCCTGCTTTTCAGCCACTATTTCGCAAGAGCAAGTCGATGACTAAAAAACTCCATATTAAAACCTGGGGCTGTCA
GATGAACGAGTACGATTCATCGAAGATGGCCGATCTGCTGGACGCCACCCACGGCTATCAACTGACCGACGTGGCGGAAG
AGGCGGATGTGCTGCTGTTGAATACCTGCTCAATCCGCGAGAAGGCTCAGGAAAAAGTCTTCCATCAATTAGGTCGCTGG
AGACTGTTAAAAGAGAAAAATCCCGATCTGATTATCGGCGTCGGCGGCTGTGTGGCTTCCCAGGAAGGCGAGCACATTCG
TCAACGCGCCCATTACGTCGACATTATTTTTGGCCCGCAAACCTTACACCGCCTGCCGGAGATGATTAACTCCGTGCGTG
GCGACCGCAGCCCGGTGGTCGATATCAGCTTCCCGGAAATCGAGAAGTTCGATCGTTTGCCGGAGCCGCGCGCCGAAGGC
CCGACCGCTTTCGTTTCTATCATGGAAGGCTGCAATAAATATTGTACTTACTGCGTGGTACCGTATACCCGCGGTGAAGA
AGTCAGCCGTCCCTCAGACGATATCCTGTTCGAAATCGCCCAGTTAGCGGCGCAGGGCGTGCGTGAGGTCAACCTGCTGG
GCCAGAACGTTAACGCCTGGCGCGGAGAAAACTACGACGGCACCACCGGCACCTTTGCCGATCTGCTGCGTCTGGTTGCC
GCCATCGACGGCATTGACCGTATTCGCTTTACCACCAGCCATCCGATTGAGTTCACCGACGATATTATCGAGGTGTACCG
CGATACGCCGGAGCTGGTCAGTTTCCTGCATCTGCCGGTACAGAGCGGTTCCGATCGCGTGTTGAATCTGATGGGGCGCA
CCCACACTGCGCTGGAATATAAAGCAATCATCCGTAAACTGCGCGCGGCGCGGCCGGACATTCAGATAAGCTCTGACTTT
ATCGTCGGTTTCCCAGGCGAAACTACCGATGATTTCGAAAAAACCATGAAGCTTATCGCCGACGTCAACTTTGATATGAG
CTACAGCTTTATCTTCTCTGCGCGTCCGGGAACGCCGGCTGCCGATATGGTTGATGACGTACCGGAAGAAGAGAAAAAGC
AGCGTCTGTATATTCTGCAAGAGCGTATCAATCAGCAGGCGATGGCATGGAGCCGCCGTATGCTCGGCACGACACAGCGC
ATTCTGGTGGAAGGCACGTCGCGTAAAAACATTATGGAACTGTCTGGCCGTACCGAAAACAATCGGGTGGTGAACTTTGA
AGGCACGCCGGAGATGATTGGTAAGTTTGTCGATGTGGAAATTACTGACGTCTATCCGAACTCCCTGCGCGGAAAAGTGG
TGCGTACCGAAGATGAAATGGGTCTGCGCGTCGCCGAAACGCCGGAGTCCGTCATTGCCCGTACCCGTAAAGAAAATGAG
CTGGGCGTAGGATTCTACCAGCCTTAA

Upstream 100 bases:

>100_bases
GTCATTCATCCTGTTACGTTTTAGACAAGTTTACAGGATTTTTTACCCATTAGGGCTGACGTCAGTCATACTGGTCACAA
CCGCGCCAAAGCATTACACT

Downstream 100 bases:

>100_bases
TCCTGCAGGAAAGGCAATAATGCCCGATGGCGCTACGCCTGTCGGGCTTCCTGGTCATGGCCTGTCGCCCGACGGGCCTT
GCAATTTTCTCTCTTCATCC

Product: (dimethylallyl)adenosine tRNA methylthiotransferase

Products: NA

Alternate protein names: tRNA-i(6)A37 methylthiotransferase [H]

Number of amino acids: Translated: 488; Mature: 488

Protein sequence:

>488_residues
MRPAFQPLFRKSKSMTKKLHIKTWGCQMNEYDSSKMADLLDATHGYQLTDVAEEADVLLLNTCSIREKAQEKVFHQLGRW
RLLKEKNPDLIIGVGGCVASQEGEHIRQRAHYVDIIFGPQTLHRLPEMINSVRGDRSPVVDISFPEIEKFDRLPEPRAEG
PTAFVSIMEGCNKYCTYCVVPYTRGEEVSRPSDDILFEIAQLAAQGVREVNLLGQNVNAWRGENYDGTTGTFADLLRLVA
AIDGIDRIRFTTSHPIEFTDDIIEVYRDTPELVSFLHLPVQSGSDRVLNLMGRTHTALEYKAIIRKLRAARPDIQISSDF
IVGFPGETTDDFEKTMKLIADVNFDMSYSFIFSARPGTPAADMVDDVPEEEKKQRLYILQERINQQAMAWSRRMLGTTQR
ILVEGTSRKNIMELSGRTENNRVVNFEGTPEMIGKFVDVEITDVYPNSLRGKVVRTEDEMGLRVAETPESVIARTRKENE
LGVGFYQP

Sequences:

>Translated_488_residues
MRPAFQPLFRKSKSMTKKLHIKTWGCQMNEYDSSKMADLLDATHGYQLTDVAEEADVLLLNTCSIREKAQEKVFHQLGRW
RLLKEKNPDLIIGVGGCVASQEGEHIRQRAHYVDIIFGPQTLHRLPEMINSVRGDRSPVVDISFPEIEKFDRLPEPRAEG
PTAFVSIMEGCNKYCTYCVVPYTRGEEVSRPSDDILFEIAQLAAQGVREVNLLGQNVNAWRGENYDGTTGTFADLLRLVA
AIDGIDRIRFTTSHPIEFTDDIIEVYRDTPELVSFLHLPVQSGSDRVLNLMGRTHTALEYKAIIRKLRAARPDIQISSDF
IVGFPGETTDDFEKTMKLIADVNFDMSYSFIFSARPGTPAADMVDDVPEEEKKQRLYILQERINQQAMAWSRRMLGTTQR
ILVEGTSRKNIMELSGRTENNRVVNFEGTPEMIGKFVDVEITDVYPNSLRGKVVRTEDEMGLRVAETPESVIARTRKENE
LGVGFYQP
>Mature_488_residues
MRPAFQPLFRKSKSMTKKLHIKTWGCQMNEYDSSKMADLLDATHGYQLTDVAEEADVLLLNTCSIREKAQEKVFHQLGRW
RLLKEKNPDLIIGVGGCVASQEGEHIRQRAHYVDIIFGPQTLHRLPEMINSVRGDRSPVVDISFPEIEKFDRLPEPRAEG
PTAFVSIMEGCNKYCTYCVVPYTRGEEVSRPSDDILFEIAQLAAQGVREVNLLGQNVNAWRGENYDGTTGTFADLLRLVA
AIDGIDRIRFTTSHPIEFTDDIIEVYRDTPELVSFLHLPVQSGSDRVLNLMGRTHTALEYKAIIRKLRAARPDIQISSDF
IVGFPGETTDDFEKTMKLIADVNFDMSYSFIFSARPGTPAADMVDDVPEEEKKQRLYILQERINQQAMAWSRRMLGTTQR
ILVEGTSRKNIMELSGRTENNRVVNFEGTPEMIGKFVDVEITDVYPNSLRGKVVRTEDEMGLRVAETPESVIARTRKENE
LGVGFYQP

Specific function: Catalyzes the methylthiolation of N6- (dimethylallyl)adenosine (i(6)A), leading to the formation of 2- methylthio-N6-(dimethylallyl)adenosine (ms(2)i(6)A) at position 37 in tRNAs that read codons beginning with uridine [H]

COG id: COG0621

COG function: function code J; 2-methylthioadenine synthetase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 TRAM domain [H]

Homologues:

Organism=Homo sapiens, GI28872784, Length=478, Percent_Identity=37.0292887029289, Blast_Score=288, Evalue=6e-78,
Organism=Homo sapiens, GI28872782, Length=478, Percent_Identity=37.0292887029289, Blast_Score=288, Evalue=6e-78,
Organism=Homo sapiens, GI93277076, Length=456, Percent_Identity=30.2631578947368, Blast_Score=177, Evalue=1e-44,
Organism=Escherichia coli, GI1786882, Length=474, Percent_Identity=98.3122362869198, Blast_Score=973, Evalue=0.0,
Organism=Escherichia coli, GI1787057, Length=385, Percent_Identity=27.5324675324675, Blast_Score=147, Evalue=2e-36,
Organism=Caenorhabditis elegans, GI17553146, Length=462, Percent_Identity=33.1168831168831, Blast_Score=247, Evalue=1e-65,
Organism=Caenorhabditis elegans, GI71996771, Length=345, Percent_Identity=27.8260869565217, Blast_Score=118, Evalue=6e-27,
Organism=Drosophila melanogaster, GI21356207, Length=433, Percent_Identity=37.1824480369515, Blast_Score=277, Evalue=1e-74,
Organism=Drosophila melanogaster, GI19922432, Length=462, Percent_Identity=29.2207792207792, Blast_Score=162, Evalue=4e-40,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006638
- InterPro:   IPR005839
- InterPro:   IPR020612
- InterPro:   IPR013848
- InterPro:   IPR006463
- InterPro:   IPR007197
- InterPro:   IPR002792 [H]

Pfam domain/function: PF04055 Radical_SAM; PF01938 TRAM; PF00919 UPF0004 [H]

EC number: NA

Molecular weight: Translated: 55392; Mature: 55392

Theoretical pI: Translated: 5.32; Mature: 5.32

Prosite motif: PS50926 TRAM ; PS01278 UPF0004

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRPAFQPLFRKSKSMTKKLHIKTWGCQMNEYDSSKMADLLDATHGYQLTDVAEEADVLLL
CCCCHHHHHHHHHHHHHEEEEEEECCCCCCCCHHHHHHHHHHCCCCEEHHHHCCCCEEEE
NTCSIREKAQEKVFHQLGRWRLLKEKNPDLIIGVGGCVASQEGEHIRQRAHYVDIIFGPQ
ECCHHHHHHHHHHHHHHHHEEEECCCCCCEEEECCCCCCCCCHHHHHHHCCEEEEEECHH
TLHRLPEMINSVRGDRSPVVDISFPEIEKFDRLPEPRAEGPTAFVSIMEGCNKYCTYCVV
HHHHHHHHHHHHCCCCCCEEECCCCCHHHHHCCCCCCCCCHHHHHHHHHHHHHHEEEEEE
PYTRGEEVSRPSDDILFEIAQLAAQGVREVNLLGQNVNAWRGENYDGTTGTFADLLRLVA
ECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHH
AIDGIDRIRFTTSHPIEFTDDIIEVYRDTPELVSFLHLPVQSGSDRVLNLMGRTHTALEY
HHCCCHHEEEECCCCCCHHHHHHHHHHCCHHHHHHHHCCCCCCCHHHHHHHCCHHHHHHH
KAIIRKLRAARPDIQISSDFIVGFPGETTDDFEKTMKLIADVNFDMSYSFIFSARPGTPA
HHHHHHHHHCCCCCEECCCEEEECCCCCHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCH
ADMVDDVPEEEKKQRLYILQERINQQAMAWSRRMLGTTQRILVEGTSRKNIMELSGRTEN
HHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCHHEECCCCCC
NRVVNFEGTPEMIGKFVDVEITDVYPNSLRGKVVRTEDEMGLRVAETPESVIARTRKENE
CEEEEECCCHHHHCCEECEEEEECCCCCCCCCEEEEHHHCCCEEECCCHHHHHHHCCCCC
LGVGFYQP
CCCCCCCC
>Mature Secondary Structure
MRPAFQPLFRKSKSMTKKLHIKTWGCQMNEYDSSKMADLLDATHGYQLTDVAEEADVLLL
CCCCHHHHHHHHHHHHHEEEEEEECCCCCCCCHHHHHHHHHHCCCCEEHHHHCCCCEEEE
NTCSIREKAQEKVFHQLGRWRLLKEKNPDLIIGVGGCVASQEGEHIRQRAHYVDIIFGPQ
ECCHHHHHHHHHHHHHHHHEEEECCCCCCEEEECCCCCCCCCHHHHHHHCCEEEEEECHH
TLHRLPEMINSVRGDRSPVVDISFPEIEKFDRLPEPRAEGPTAFVSIMEGCNKYCTYCVV
HHHHHHHHHHHHCCCCCCEEECCCCCHHHHHCCCCCCCCCHHHHHHHHHHHHHHEEEEEE
PYTRGEEVSRPSDDILFEIAQLAAQGVREVNLLGQNVNAWRGENYDGTTGTFADLLRLVA
ECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHH
AIDGIDRIRFTTSHPIEFTDDIIEVYRDTPELVSFLHLPVQSGSDRVLNLMGRTHTALEY
HHCCCHHEEEECCCCCCHHHHHHHHHHCCHHHHHHHHCCCCCCCHHHHHHHCCHHHHHHH
KAIIRKLRAARPDIQISSDFIVGFPGETTDDFEKTMKLIADVNFDMSYSFIFSARPGTPA
HHHHHHHHHCCCCCEECCCEEEECCCCCHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCH
ADMVDDVPEEEKKQRLYILQERINQQAMAWSRRMLGTTQRILVEGTSRKNIMELSGRTEN
HHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCHHEECCCCCC
NRVVNFEGTPEMIGKFVDVEITDVYPNSLRGKVVRTEDEMGLRVAETPESVIARTRKENE
CEEEEECCCHHHHCCEECEEEEECCCCCCCCCEEEEHHHCCCEEECCCHHHHHHHCCCCC
LGVGFYQP
CCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA