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The map label for this gene is purB [H]

Identifier: 29142122

GI number: 29142122

Start: 1750841

End: 1752211

Strand: Direct

Name: purB [H]

Synonym: t1688

Alternate gene names: 29142122

Gene position: 1750841-1752211 (Clockwise)

Preceding gene: 29142121

Following gene: 29142123

Centisome position: 36.54

GC content: 51.64

Gene sequence:

>1371_bases
ATGGAATTATCCTCACTGACCGCCGTTTCCCCTGTCGATGGACGCTACGGCGATAAAGTCAGCGCGCTGCGCGGAATTTT
TAGCGAATACGGTTTGCTGAAATTTCGTGTACAAGTCGAAGTACGTTGGCTGCAGAAATTAGCCGCGCACGCAGCGATCA
AGGAAGTTCCTGCTTTTGCTGCCGACGCAAACGGTTACCTGGATACGCTTGTGGCAAACTTCAATGAAGAAGATGCCGCG
CGCATTAAAACCATTGAGCGCACCACGAATCACGATGTGAAAGCGGTTGAGTATTTTCTGAAAGAAAAAGCCGCCGCGAT
CCCGGCGCTACATGACGTTTCCGAATTTATCCACTTTGCCTGCACCTCTGAGGATATTAACAACCTGTCGCACGCGTTAA
TGCTCAAAACTGCGCGCGATGAAGTGATCCTGCCTTACTGGCGTCAGGTGATTAACGCGGTTAAAGATCTCGCCACGCAG
TATCGCGATATTCCTCTGCTCTCCCGCACCCACGGCCAGCCGGCAACACCTTCCACTCTGGGTAAAGAGATGGCGAACGT
GGCGTATCGGATGGAGCGTCAGTTCCGCCAGCTCAATCAGGTGGAGATCCTCGGTAAAATTAACGGCGCCGTAGGCAACT
ATAACGCGCATATCGCCGCCTATCCGGAAGTTGACTGGCATCAGTTCAGCGAAGAGTTCGTCACCTCGCTGGGCATCCAG
TGGAACCCTTACACCACCCAGATTGAACCGCATGATTATATTGCGGAACTGTTTGACTGTATCGCGCGCTTTAACACCAT
CCTGATCGATTTCGATCGCGATGTCTGGGGCTATATTGCGTTGAACCATTTCAAACAGAAAACCATCGCCGGAGAGATCG
GTTCTTCTACCATGCCGCATAAAGTTAACCCCATTGACTTTGAAAACTCAGAAGGCAACCTCGGTCTGTCTAATGCAGTG
TTGCACCATCTGGCAAACAAACTGCCGGTTTCCCGCTGGCAGCGCGATCTGACCGACTCAACCGTCCTGCGTAACCTGGG
TGTCGGCATCGGCTATGCGCTTATCGCTTATCAGTCGACCCTGAAGGGCGTCAGCAAGCTGGAAGTGAACCGCGATCATC
TGCTTGACGAACTGGATCACAACTGGGAAGTATTAGCCGAACCGATCCAGACCGTCATGCGCCGCTATGGCATTGAAAAA
CCATATGAAAAACTGAAAGAGTTGACCCGTGGCAAGCGTGTCGATGCCGAAGGAATGAAACAGCTTATTGATAGTCTGGC
CCTGCCGGAAGCAGAAAAAACGCGCCTTAAAGCCATGACGCCGGCAAATTATATCGGTCGCGCTGTGAATCTGGTCGACG
AACTTAAATAA

Upstream 100 bases:

>100_bases
TCAGGTAGGCGGTGGTCGCCTACAGCTTATGTTTTCTCGTCATCGCCTGACGACTCAGGCAAAACAAATTCTTGCTTATT
TAACCCCGGAGTTATGATCT

Downstream 100 bases:

>100_bases
TGTGTGCCTCACCCTCTTTTCTTCAGAAAGAGGGTGACTATTTGTCTGGTTTATTAACTGTTTATCCCCAAAGCACCATA
ATCAACGCTAGACTGTTCTT

Product: adenylosuccinate lyase

Products: NA

Alternate protein names: ASL; Adenylosuccinase; ASase [H]

Number of amino acids: Translated: 456; Mature: 456

Protein sequence:

>456_residues
MELSSLTAVSPVDGRYGDKVSALRGIFSEYGLLKFRVQVEVRWLQKLAAHAAIKEVPAFAADANGYLDTLVANFNEEDAA
RIKTIERTTNHDVKAVEYFLKEKAAAIPALHDVSEFIHFACTSEDINNLSHALMLKTARDEVILPYWRQVINAVKDLATQ
YRDIPLLSRTHGQPATPSTLGKEMANVAYRMERQFRQLNQVEILGKINGAVGNYNAHIAAYPEVDWHQFSEEFVTSLGIQ
WNPYTTQIEPHDYIAELFDCIARFNTILIDFDRDVWGYIALNHFKQKTIAGEIGSSTMPHKVNPIDFENSEGNLGLSNAV
LHHLANKLPVSRWQRDLTDSTVLRNLGVGIGYALIAYQSTLKGVSKLEVNRDHLLDELDHNWEVLAEPIQTVMRRYGIEK
PYEKLKELTRGKRVDAEGMKQLIDSLALPEAEKTRLKAMTPANYIGRAVNLVDELK

Sequences:

>Translated_456_residues
MELSSLTAVSPVDGRYGDKVSALRGIFSEYGLLKFRVQVEVRWLQKLAAHAAIKEVPAFAADANGYLDTLVANFNEEDAA
RIKTIERTTNHDVKAVEYFLKEKAAAIPALHDVSEFIHFACTSEDINNLSHALMLKTARDEVILPYWRQVINAVKDLATQ
YRDIPLLSRTHGQPATPSTLGKEMANVAYRMERQFRQLNQVEILGKINGAVGNYNAHIAAYPEVDWHQFSEEFVTSLGIQ
WNPYTTQIEPHDYIAELFDCIARFNTILIDFDRDVWGYIALNHFKQKTIAGEIGSSTMPHKVNPIDFENSEGNLGLSNAV
LHHLANKLPVSRWQRDLTDSTVLRNLGVGIGYALIAYQSTLKGVSKLEVNRDHLLDELDHNWEVLAEPIQTVMRRYGIEK
PYEKLKELTRGKRVDAEGMKQLIDSLALPEAEKTRLKAMTPANYIGRAVNLVDELK
>Mature_456_residues
MELSSLTAVSPVDGRYGDKVSALRGIFSEYGLLKFRVQVEVRWLQKLAAHAAIKEVPAFAADANGYLDTLVANFNEEDAA
RIKTIERTTNHDVKAVEYFLKEKAAAIPALHDVSEFIHFACTSEDINNLSHALMLKTARDEVILPYWRQVINAVKDLATQ
YRDIPLLSRTHGQPATPSTLGKEMANVAYRMERQFRQLNQVEILGKINGAVGNYNAHIAAYPEVDWHQFSEEFVTSLGIQ
WNPYTTQIEPHDYIAELFDCIARFNTILIDFDRDVWGYIALNHFKQKTIAGEIGSSTMPHKVNPIDFENSEGNLGLSNAV
LHHLANKLPVSRWQRDLTDSTVLRNLGVGIGYALIAYQSTLKGVSKLEVNRDHLLDELDHNWEVLAEPIQTVMRRYGIEK
PYEKLKELTRGKRVDAEGMKQLIDSLALPEAEKTRLKAMTPANYIGRAVNLVDELK

Specific function: De novo purine biosynthesis; eighth step. [C]

COG id: COG0015

COG function: function code F; Adenylosuccinate lyase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lyase 1 family. Adenylosuccinate lyase subfamily [H]

Homologues:

Organism=Escherichia coli, GI1787376, Length=456, Percent_Identity=94.2982456140351, Blast_Score=902, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013539
- InterPro:   IPR000362
- InterPro:   IPR020557
- InterPro:   IPR008948
- InterPro:   IPR022761
- InterPro:   IPR004769 [H]

Pfam domain/function: PF08328 ASL_C; PF00206 Lyase_1 [H]

EC number: =4.3.2.2 [H]

Molecular weight: Translated: 51498; Mature: 51498

Theoretical pI: Translated: 6.35; Mature: 6.35

Prosite motif: PS00163 FUMARATE_LYASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MELSSLTAVSPVDGRYGDKVSALRGIFSEYGLLKFRVQVEVRWLQKLAAHAAIKEVPAFA
CCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCEEEEEEHHHHHHHHHHHHHHHHHHCCCHH
ADANGYLDTLVANFNEEDAARIKTIERTTNHDVKAVEYFLKEKAAAIPALHDVSEFIHFA
CCCCCHHHHHHHCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHH
CTSEDINNLSHALMLKTARDEVILPYWRQVINAVKDLATQYRDIPLLSRTHGQPATPSTL
CCHHHHHHHHHHHHHHHCCCCEEHHHHHHHHHHHHHHHHHHHCCCCHHCCCCCCCCHHHH
GKEMANVAYRMERQFRQLNQVEILGKINGAVGNYNAHIAAYPEVDWHQFSEEFVTSLGIQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEECCCCHHHHHHHHHHHHCCCC
WNPYTTQIEPHDYIAELFDCIARFNTILIDFDRDVWGYIALNHFKQKTIAGEIGSSTMPH
CCCCCCCCCCHHHHHHHHHHHHHHCEEEEEECCCHHHHHHHHHHHHHHHHHHCCCCCCCC
KVNPIDFENSEGNLGLSNAVLHHLANKLPVSRWQRDLTDSTVLRNLGVGIGYALIAYQST
CCCCCCCCCCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHH
LKGVSKLEVNRDHLLDELDHNWEVLAEPIQTVMRRYGIEKPYEKLKELTRGKRVDAEGMK
HHHHHHHHCCHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCHHHHH
QLIDSLALPEAEKTRLKAMTPANYIGRAVNLVDELK
HHHHHHCCCCHHHHHHHHCCCHHHHHHHHHHHHHCC
>Mature Secondary Structure
MELSSLTAVSPVDGRYGDKVSALRGIFSEYGLLKFRVQVEVRWLQKLAAHAAIKEVPAFA
CCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCEEEEEEHHHHHHHHHHHHHHHHHHCCCHH
ADANGYLDTLVANFNEEDAARIKTIERTTNHDVKAVEYFLKEKAAAIPALHDVSEFIHFA
CCCCCHHHHHHHCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHH
CTSEDINNLSHALMLKTARDEVILPYWRQVINAVKDLATQYRDIPLLSRTHGQPATPSTL
CCHHHHHHHHHHHHHHHCCCCEEHHHHHHHHHHHHHHHHHHHCCCCHHCCCCCCCCHHHH
GKEMANVAYRMERQFRQLNQVEILGKINGAVGNYNAHIAAYPEVDWHQFSEEFVTSLGIQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEECCCCHHHHHHHHHHHHCCCC
WNPYTTQIEPHDYIAELFDCIARFNTILIDFDRDVWGYIALNHFKQKTIAGEIGSSTMPH
CCCCCCCCCCHHHHHHHHHHHHHHCEEEEEECCCHHHHHHHHHHHHHHHHHHCCCCCCCC
KVNPIDFENSEGNLGLSNAVLHHLANKLPVSRWQRDLTDSTVLRNLGVGIGYALIAYQST
CCCCCCCCCCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHH
LKGVSKLEVNRDHLLDELDHNWEVLAEPIQTVMRRYGIEKPYEKLKELTRGKRVDAEGMK
HHHHHHHHCCHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCHHHHH
QLIDSLALPEAEKTRLKAMTPANYIGRAVNLVDELK
HHHHHHCCCCHHHHHHHHCCCHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]