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The map label for this gene is ppsA [H]

Identifier: 29141694

GI number: 29141694

Start: 1296214

End: 1298592

Strand: Direct

Name: ppsA [H]

Synonym: t1230

Alternate gene names: 29141694

Gene position: 1296214-1298592 (Clockwise)

Preceding gene: 29141690

Following gene: 29141697

Centisome position: 27.05

GC content: 55.44

Gene sequence:

>2379_bases
ATGTCCAACAATGGCTCGTCACCGCTGGTGCTTTGGTATAACCAACTCGGCATGAATGATGTAGACAGAGTTGGGGGCAA
AAATGCCTCCCTGGGTGAAATGATTACTAACCTTTCCGGCATGGGCGTATCCGTTCCGAATGGTTTTGCAACCACTGCCG
ATGCGTTTAACCAGTTTTTGGATCAAAGCGGCGTAAACCAGCGCATTTATGAGCTGCTGGATAAGACAGATATTGATGAT
GTCTCCCAGCTGGCGAAAGCGGGCGCGCAGATCCGCCAGTGGATTATCGACACTCCTTTCCAGCCGGCGCTGGAAAACGC
CATCCGCGATGCCTATGCGCAGCTTTCGTCGGATGATGAAAATGCCTCTTTTGCCGTGCGTTCCTCCGCCACGGCGGAAG
ACATGCCAGATGCTTCGTTTGCGGGTCAACAGGAAACGTTTCTTAACGTTCAGGGCTTCGATGCGGTACTGGTGGCAGTG
AAACACGTATTTGCTTCTCTGTTTAACGATCGCGCCATCTCTTATCGCGTTCACCAGGGATATGATCATCGCGGCGTAGC
GCTTTCCGCTGGCGTGCAGCGGATGGTTCGCTCCGATCTCGCCTCGTCAGGGGTGATGTTCTCTATCGATACCGAATCCG
GCTTTGACCAGGTGGTCTTTATCACCTCGGCATGGGGACTGGGTGAAATGGTGGTGCAGGGCGCGGTTAACCCGGATGAA
TTTTATGTCCATAAGCCAACCCTGGCGGCAGGCCGTCCGGCCATCGTACGCCGCACCATGGGGTCGAAAAAAATCCGCAT
GGTTTACGCCGCCACCCAGGAGCACGGTAAGCAAGTGAAGATTGAGGATGTGCCGCAGGAACAGCGCGACATTTTCTCGC
TCACCAACGAAGAAGTGCAGGAACTGGCGAAACAAGCGGTGCAGATTGAGCAGCACTATGGTCGTCCGATGGATATCGAA
TGGGCGAAAGACGGTCATACCGGTAAGCTCTTTATTGTGCAGGCACGTCCGGAAACCGTGCGCTCTCGTGGCCAGGTGAT
GGAGCGTTATACGTTACATGCGCAGGGGAAAATCATTGCCGAAGGTCGTGCCATTGGGCATCGTATCGGCGCAGGTACGG
TAAAAGTTATTCACGATATCGGCGAAATGAACCGTATTGAGCCTGGCGATGTGCTTGTCACCGATATGACCGACCCGGAC
TGGGAACCGATCATGAAAAAAGCGGCGGCGATTGTCACTAACCGTGGCGGGCGCACCTGTCACGCGGCGATTATCGCCCG
CGAATTGGGCATTCCGGCGGTAGTCGGCTGCGGCGACGCCACCGAACGCATGAAAGATGGCGAAAAAGTAACCGTCTCCT
GTGCGGAAGGTGATACCGGTTATGTGTATGCCGACATGCTGGATTTCAGCGTAAAAAGCTCCAGCGTAGATACCATGCCA
GAGCTACCGCTGAAGGTCATGATGAACGTCGGCAACCCGGACAGGGCATTTGACTTTGCCTGCCTGCCGAATGAAGGCGT
AGGTCTGGCGCGACTGGAATTCATCATTAACCGTATGATTGGCGTTCACCCGCGCGCGTTGCTGGAGTTTGACGACCAGG
ATGCCGCGCTGCAAAACGACATTCGTGAGATGATGAAAGGGTTTGACTCGCCGCGTGAATTTTACGTTGGTCGACTGACG
GAAGGGATCGCGACGTTAGGCGCCGCCTTCTACCCGAAACGCATTATCGTTCGCCTGTCTGACTTTAAGTCCAACGAATA
CGCCAACCTGGTCGGCGGCGAACGTTATGAGCCGCATGAAGAAAACCCGATGCTGGGCTTCCGCGGCGCGGGTCGTTACG
TCGCAGAAAGTTTCCGCGATTGTTTTGCGCTGGAATGCGAAGCGGTGAAACGCGTGCGCAACGATATGGGCCTTACCAAC
GTCGAAATCATGATCCCGTTCGTGCGGACGGTCGACCAGGCGAAAGCGGTCATCGAAGAGCTGGCGCGCCAGGGACTTAA
ACGCGGCGAGAACGGGCTGAAGATCATCATGATGTGCGAGATTCCGTCCAATGCCTTGCTGGCTGAACAGTTCCTCGAGT
ATTTTGATGGCTTCTCGATTGGTTCTAACGACATGACGCAGCTGGCGCTGGGGCTGGATCGCGATTCCGGCGTGGTGTCT
GAACTGTTTGACGAGCGTAACGACGCGGTGAAAGCGCTGCTATCGATGGCGATCCGCGCGGCGAAAAAACAGGGCAAATA
TGTTGGAATTTGCGGCCAGGGCCCGTCCGACCATGAAGACTTTGCCGCCTGGCTGATGGAAGAGGGGATCGACAGCCTGT
CGCTGAACCCGGACACCGTGGTGCAGACCTGGCTGAGTCTGGCGGAACTGAAGAAGTAA

Upstream 100 bases:

>100_bases
TGAACGATTCACCGTTTTTTTCGCGGGTTTAAGTATGCCAGGATAAATACGCAACTATGTGTTTCTTAAACTCGTTCATT
TATCACAAAAGGATTGTTCG

Downstream 100 bases:

>100_bases
ACCGCGACCAGATAACGCTATGCTTATCCGGCCTGCACAAGAAACATGTTGTGTCGGCCGGATAGGGCGTAAGTTGCCCG
ACGCCTGCATAATGCCGGAT

Product: phosphoenolpyruvate synthase

Products: NA

Alternate protein names: PEP synthase; Pyruvate, water dikinase [H]

Number of amino acids: Translated: 792; Mature: 791

Protein sequence:

>792_residues
MSNNGSSPLVLWYNQLGMNDVDRVGGKNASLGEMITNLSGMGVSVPNGFATTADAFNQFLDQSGVNQRIYELLDKTDIDD
VSQLAKAGAQIRQWIIDTPFQPALENAIRDAYAQLSSDDENASFAVRSSATAEDMPDASFAGQQETFLNVQGFDAVLVAV
KHVFASLFNDRAISYRVHQGYDHRGVALSAGVQRMVRSDLASSGVMFSIDTESGFDQVVFITSAWGLGEMVVQGAVNPDE
FYVHKPTLAAGRPAIVRRTMGSKKIRMVYAATQEHGKQVKIEDVPQEQRDIFSLTNEEVQELAKQAVQIEQHYGRPMDIE
WAKDGHTGKLFIVQARPETVRSRGQVMERYTLHAQGKIIAEGRAIGHRIGAGTVKVIHDIGEMNRIEPGDVLVTDMTDPD
WEPIMKKAAAIVTNRGGRTCHAAIIARELGIPAVVGCGDATERMKDGEKVTVSCAEGDTGYVYADMLDFSVKSSSVDTMP
ELPLKVMMNVGNPDRAFDFACLPNEGVGLARLEFIINRMIGVHPRALLEFDDQDAALQNDIREMMKGFDSPREFYVGRLT
EGIATLGAAFYPKRIIVRLSDFKSNEYANLVGGERYEPHEENPMLGFRGAGRYVAESFRDCFALECEAVKRVRNDMGLTN
VEIMIPFVRTVDQAKAVIEELARQGLKRGENGLKIIMMCEIPSNALLAEQFLEYFDGFSIGSNDMTQLALGLDRDSGVVS
ELFDERNDAVKALLSMAIRAAKKQGKYVGICGQGPSDHEDFAAWLMEEGIDSLSLNPDTVVQTWLSLAELKK

Sequences:

>Translated_792_residues
MSNNGSSPLVLWYNQLGMNDVDRVGGKNASLGEMITNLSGMGVSVPNGFATTADAFNQFLDQSGVNQRIYELLDKTDIDD
VSQLAKAGAQIRQWIIDTPFQPALENAIRDAYAQLSSDDENASFAVRSSATAEDMPDASFAGQQETFLNVQGFDAVLVAV
KHVFASLFNDRAISYRVHQGYDHRGVALSAGVQRMVRSDLASSGVMFSIDTESGFDQVVFITSAWGLGEMVVQGAVNPDE
FYVHKPTLAAGRPAIVRRTMGSKKIRMVYAATQEHGKQVKIEDVPQEQRDIFSLTNEEVQELAKQAVQIEQHYGRPMDIE
WAKDGHTGKLFIVQARPETVRSRGQVMERYTLHAQGKIIAEGRAIGHRIGAGTVKVIHDIGEMNRIEPGDVLVTDMTDPD
WEPIMKKAAAIVTNRGGRTCHAAIIARELGIPAVVGCGDATERMKDGEKVTVSCAEGDTGYVYADMLDFSVKSSSVDTMP
ELPLKVMMNVGNPDRAFDFACLPNEGVGLARLEFIINRMIGVHPRALLEFDDQDAALQNDIREMMKGFDSPREFYVGRLT
EGIATLGAAFYPKRIIVRLSDFKSNEYANLVGGERYEPHEENPMLGFRGAGRYVAESFRDCFALECEAVKRVRNDMGLTN
VEIMIPFVRTVDQAKAVIEELARQGLKRGENGLKIIMMCEIPSNALLAEQFLEYFDGFSIGSNDMTQLALGLDRDSGVVS
ELFDERNDAVKALLSMAIRAAKKQGKYVGICGQGPSDHEDFAAWLMEEGIDSLSLNPDTVVQTWLSLAELKK
>Mature_791_residues
SNNGSSPLVLWYNQLGMNDVDRVGGKNASLGEMITNLSGMGVSVPNGFATTADAFNQFLDQSGVNQRIYELLDKTDIDDV
SQLAKAGAQIRQWIIDTPFQPALENAIRDAYAQLSSDDENASFAVRSSATAEDMPDASFAGQQETFLNVQGFDAVLVAVK
HVFASLFNDRAISYRVHQGYDHRGVALSAGVQRMVRSDLASSGVMFSIDTESGFDQVVFITSAWGLGEMVVQGAVNPDEF
YVHKPTLAAGRPAIVRRTMGSKKIRMVYAATQEHGKQVKIEDVPQEQRDIFSLTNEEVQELAKQAVQIEQHYGRPMDIEW
AKDGHTGKLFIVQARPETVRSRGQVMERYTLHAQGKIIAEGRAIGHRIGAGTVKVIHDIGEMNRIEPGDVLVTDMTDPDW
EPIMKKAAAIVTNRGGRTCHAAIIARELGIPAVVGCGDATERMKDGEKVTVSCAEGDTGYVYADMLDFSVKSSSVDTMPE
LPLKVMMNVGNPDRAFDFACLPNEGVGLARLEFIINRMIGVHPRALLEFDDQDAALQNDIREMMKGFDSPREFYVGRLTE
GIATLGAAFYPKRIIVRLSDFKSNEYANLVGGERYEPHEENPMLGFRGAGRYVAESFRDCFALECEAVKRVRNDMGLTNV
EIMIPFVRTVDQAKAVIEELARQGLKRGENGLKIIMMCEIPSNALLAEQFLEYFDGFSIGSNDMTQLALGLDRDSGVVSE
LFDERNDAVKALLSMAIRAAKKQGKYVGICGQGPSDHEDFAAWLMEEGIDSLSLNPDTVVQTWLSLAELKK

Specific function: Catalyzes the phosphorylation of pyruvate to phosphoenolpyruvate [H]

COG id: COG0574

COG function: function code G; Phosphoenolpyruvate synthase/pyruvate phosphate dikinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PEP-utilizing enzyme family [H]

Homologues:

Organism=Escherichia coli, GI1787994, Length=792, Percent_Identity=96.7171717171717, Blast_Score=1595, Evalue=0.0,
Organism=Escherichia coli, GI1788756, Length=450, Percent_Identity=25.7777777777778, Blast_Score=111, Evalue=2e-25,
Organism=Escherichia coli, GI1788726, Length=404, Percent_Identity=26.980198019802, Blast_Score=103, Evalue=3e-23,
Organism=Escherichia coli, GI48994992, Length=190, Percent_Identity=32.6315789473684, Blast_Score=96, Evalue=1e-20,
Organism=Escherichia coli, GI1789193, Length=413, Percent_Identity=24.6973365617433, Blast_Score=76, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI17564524, Length=331, Percent_Identity=25.3776435045317, Blast_Score=77, Evalue=4e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR008279
- InterPro:   IPR006319
- InterPro:   IPR018274
- InterPro:   IPR023151
- InterPro:   IPR000121
- InterPro:   IPR002192
- InterPro:   IPR015813 [H]

Pfam domain/function: PF00391 PEP-utilizers; PF02896 PEP-utilizers_C; PF01326 PPDK_N [H]

EC number: =2.7.9.2 [H]

Molecular weight: Translated: 87166; Mature: 87035

Theoretical pI: Translated: 4.75; Mature: 4.75

Prosite motif: PS00370 PEP_ENZYMES_PHOS_SITE ; PS00742 PEP_ENZYMES_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSNNGSSPLVLWYNQLGMNDVDRVGGKNASLGEMITNLSGMGVSVPNGFATTADAFNQFL
CCCCCCCCEEEEECCCCCCHHHHCCCCCCHHHHHHHCCCCCCEECCCCCCHHHHHHHHHH
DQSGVNQRIYELLDKTDIDDVSQLAKAGAQIRQWIIDTPFQPALENAIRDAYAQLSSDDE
HHCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCC
NASFAVRSSATAEDMPDASFAGQQETFLNVQGFDAVLVAVKHVFASLFNDRAISYRVHQG
CCEEEEECCCCCCCCCCCCCCCCCCCEEECCCHHHHHHHHHHHHHHHHCCCCEEEEEECC
YDHRGVALSAGVQRMVRSDLASSGVMFSIDTESGFDQVVFITSAWGLGEMVVQGAVNPDE
CCCCCEEHHHHHHHHHHHHHHCCCEEEEEECCCCCCEEEEEEECCCHHHHHHHCCCCCCC
FYVHKPTLAAGRPAIVRRTMGSKKIRMVYAATQEHGKQVKIEDVPQEQRDIFSLTNEEVQ
EEEECCCCCCCCHHHHHHHCCCCEEEEEEEEHHHCCCEEEECCCCHHHHHHHHHCHHHHH
ELAKQAVQIEQHYGRPMDIEWAKDGHTGKLFIVQARPETVRSRGQVMERYTLHAQGKIIA
HHHHHHHHHHHHCCCCCCEEECCCCCCCEEEEEECCCHHHHHHHHHHHHHHHCCCCEEEE
EGRAIGHRIGAGTVKVIHDIGEMNRIEPGDVLVTDMTDPDWEPIMKKAAAIVTNRGGRTC
CCHHHHHHCCCCHHHHHHHHHHCCCCCCCCEEEECCCCCCHHHHHHHHHHHHCCCCCCCH
HAAIIARELGIPAVVGCGDATERMKDGEKVTVSCAEGDTGYVYADMLDFSVKSSSVDTMP
HHHHHHHHCCCCEEEECCCHHHHHCCCCEEEEEEECCCCCEEEEEEHHHHCCCCCCCCCC
ELPLKVMMNVGNPDRAFDFACLPNEGVGLARLEFIINRMIGVHPRALLEFDDQDAALQND
CCCHHHEEECCCCCCCEEEEECCCCCCCHHHHHHHHHHHHCCCCHHEEEECCCHHHHHHH
IREMMKGFDSPREFYVGRLTEGIATLGAAFYPKRIIVRLSDFKSNEYANLVGGERYEPHE
HHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCHHCCCCCCCCCCC
ENPMLGFRGAGRYVAESFRDCFALECEAVKRVRNDMGLTNVEIMIPFVRTVDQAKAVIEE
CCCCEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEHHHHHHHHHHHHHHH
LARQGLKRGENGLKIIMMCEIPSNALLAEQFLEYFDGFSIGSNDMTQLALGLDRDSGVVS
HHHHHHHCCCCCCEEEEEEECCCCHHHHHHHHHHHCCCCCCCCCHHHHHHCCCCCCCHHH
ELFDERNDAVKALLSMAIRAAKKQGKYVGICGQGPSDHEDFAAWLMEEGIDSLSLNPDTV
HHHHCHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHHHHCCCCCCCCHHHH
VQTWLSLAELKK
HHHHHHHHHHCC
>Mature Secondary Structure 
SNNGSSPLVLWYNQLGMNDVDRVGGKNASLGEMITNLSGMGVSVPNGFATTADAFNQFL
CCCCCCCEEEEECCCCCCHHHHCCCCCCHHHHHHHCCCCCCEECCCCCCHHHHHHHHHH
DQSGVNQRIYELLDKTDIDDVSQLAKAGAQIRQWIIDTPFQPALENAIRDAYAQLSSDDE
HHCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCC
NASFAVRSSATAEDMPDASFAGQQETFLNVQGFDAVLVAVKHVFASLFNDRAISYRVHQG
CCEEEEECCCCCCCCCCCCCCCCCCCEEECCCHHHHHHHHHHHHHHHHCCCCEEEEEECC
YDHRGVALSAGVQRMVRSDLASSGVMFSIDTESGFDQVVFITSAWGLGEMVVQGAVNPDE
CCCCCEEHHHHHHHHHHHHHHCCCEEEEEECCCCCCEEEEEEECCCHHHHHHHCCCCCCC
FYVHKPTLAAGRPAIVRRTMGSKKIRMVYAATQEHGKQVKIEDVPQEQRDIFSLTNEEVQ
EEEECCCCCCCCHHHHHHHCCCCEEEEEEEEHHHCCCEEEECCCCHHHHHHHHHCHHHHH
ELAKQAVQIEQHYGRPMDIEWAKDGHTGKLFIVQARPETVRSRGQVMERYTLHAQGKIIA
HHHHHHHHHHHHCCCCCCEEECCCCCCCEEEEEECCCHHHHHHHHHHHHHHHCCCCEEEE
EGRAIGHRIGAGTVKVIHDIGEMNRIEPGDVLVTDMTDPDWEPIMKKAAAIVTNRGGRTC
CCHHHHHHCCCCHHHHHHHHHHCCCCCCCCEEEECCCCCCHHHHHHHHHHHHCCCCCCCH
HAAIIARELGIPAVVGCGDATERMKDGEKVTVSCAEGDTGYVYADMLDFSVKSSSVDTMP
HHHHHHHHCCCCEEEECCCHHHHHCCCCEEEEEEECCCCCEEEEEEHHHHCCCCCCCCCC
ELPLKVMMNVGNPDRAFDFACLPNEGVGLARLEFIINRMIGVHPRALLEFDDQDAALQND
CCCHHHEEECCCCCCCEEEEECCCCCCCHHHHHHHHHHHHCCCCHHEEEECCCHHHHHHH
IREMMKGFDSPREFYVGRLTEGIATLGAAFYPKRIIVRLSDFKSNEYANLVGGERYEPHE
HHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCHHCCCCCCCCCCC
ENPMLGFRGAGRYVAESFRDCFALECEAVKRVRNDMGLTNVEIMIPFVRTVDQAKAVIEE
CCCCEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEHHHHHHHHHHHHHHH
LARQGLKRGENGLKIIMMCEIPSNALLAEQFLEYFDGFSIGSNDMTQLALGLDRDSGVVS
HHHHHHHCCCCCCEEEEEEECCCCHHHHHHHHHHHCCCCCCCCCHHHHHHCCCCCCCHHH
ELFDERNDAVKALLSMAIRAAKKQGKYVGICGQGPSDHEDFAAWLMEEGIDSLSLNPDTV
HHHHCHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHHHHCCCCCCCCHHHH
VQTWLSLAELKK
HHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1310524; 9097039; 9278503 [H]