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The map label for this gene is pfkB [H]

Identifier: 29141671

GI number: 29141671

Start: 1273532

End: 1274464

Strand: Reverse

Name: pfkB [H]

Synonym: t1206

Alternate gene names: 29141671

Gene position: 1274464-1273532 (Counterclockwise)

Preceding gene: 29141673

Following gene: 29141670

Centisome position: 26.6

GC content: 56.91

Gene sequence:

>933_bases
ATGGTACGTATCTATACGTTGACGCTTGCGCCCTCTCTCGATAGCGCAACAATCACGCCGCAAATTTATCCCGAAGGTAA
ACTGCGGTGCTCCGCGCCGGTATTCGAACCCGGCGGCGGGGGTATTAATGTCGCTCGCGCCATTGCGCATCTTGGCGGCA
CCGCGACCGCAATTTTCCCGGCTGGCGGCGCTACCGGCGAACATCTGGTCGCACTACTGGCTGACGAAAATGTCCCCGTG
TCTACCGTCGATGCGAAGGACTGGACGCGTCAGAACCTGCACGTCCACGTCGAGTCCAGCGGCGAACAATATCGTTTTGT
TATGCCCGGCGCCACGCTGGACGATGACGAATTTCGCCAGCTTGAAGAACAGGTACTGGAAATTGAATCCGGGGCAATTC
TGGTTATCAGCGGCAGCCTTCCGCCCGGCGTTAAGGTCGAGAAACTCACGCAACTGATTTCCGCCGCGCAGAAGCAAGGC
ATTCGCTGCATTATTGATAGCTCTGGCGACGCGCTCACCGCTGCGTTGGCGCTTGGCGATATCGAACTGGTGAAACCTAA
CCTGAAAGAATTGAGCGCGTTAGTCAATCGCGATCTGACGCAGCCTGATGATGTGCGTAAAGCGGCCCAGGAATTAGTGC
AAAGTGGCAAGGCTCGCCGGGTAGTGGTCTCTCTGGGGCCGCAAGGGGCGCTGGGCATAGACAGTGAGAATTGCATCCAG
GTTGTTCCTCCCCCGGTGAAAAGTCAAAGTACTGTCGGCGCAGGCGACAGTATGGTGGGCGCGATGACGTTAAAACTGGC
GCAAGACGCGTCGCTGGAAGAGATGGTGCGCTTCGGTGTGGCGGCAGGCAGCGCCGCTACGCTCAATCAAGGTACGCGTC
TGTGTTCTCATGACGATACCCAAAAAATATACGCCTATCTTTCCGCGCAATAA

Upstream 100 bases:

>100_bases
TCGTACTTCTTTAAATAAAGATCCGGAAAATCATATTGTTAATTCCTTCACTTTCCGGGGATTAAGTGCCAGACTGAAAT
CAGCCTAACAGGAGGTAACG

Downstream 100 bases:

>100_bases
TCGTTTCCCCCTCGCCGAGGGGGATACCGGAAAATCGGTTAACGGGCGGCGCATCCGCCGCTGTTAAAAACACAGTTGCT
CCCAACGAGGTGAACGATGG

Product: 6-phosphofructokinase 2

Products: NA

Alternate protein names: Phosphofructokinase-2 [H]

Number of amino acids: Translated: 310; Mature: 310

Protein sequence:

>310_residues
MVRIYTLTLAPSLDSATITPQIYPEGKLRCSAPVFEPGGGGINVARAIAHLGGTATAIFPAGGATGEHLVALLADENVPV
STVDAKDWTRQNLHVHVESSGEQYRFVMPGATLDDDEFRQLEEQVLEIESGAILVISGSLPPGVKVEKLTQLISAAQKQG
IRCIIDSSGDALTAALALGDIELVKPNLKELSALVNRDLTQPDDVRKAAQELVQSGKARRVVVSLGPQGALGIDSENCIQ
VVPPPVKSQSTVGAGDSMVGAMTLKLAQDASLEEMVRFGVAAGSAATLNQGTRLCSHDDTQKIYAYLSAQ

Sequences:

>Translated_310_residues
MVRIYTLTLAPSLDSATITPQIYPEGKLRCSAPVFEPGGGGINVARAIAHLGGTATAIFPAGGATGEHLVALLADENVPV
STVDAKDWTRQNLHVHVESSGEQYRFVMPGATLDDDEFRQLEEQVLEIESGAILVISGSLPPGVKVEKLTQLISAAQKQG
IRCIIDSSGDALTAALALGDIELVKPNLKELSALVNRDLTQPDDVRKAAQELVQSGKARRVVVSLGPQGALGIDSENCIQ
VVPPPVKSQSTVGAGDSMVGAMTLKLAQDASLEEMVRFGVAAGSAATLNQGTRLCSHDDTQKIYAYLSAQ
>Mature_310_residues
MVRIYTLTLAPSLDSATITPQIYPEGKLRCSAPVFEPGGGGINVARAIAHLGGTATAIFPAGGATGEHLVALLADENVPV
STVDAKDWTRQNLHVHVESSGEQYRFVMPGATLDDDEFRQLEEQVLEIESGAILVISGSLPPGVKVEKLTQLISAAQKQG
IRCIIDSSGDALTAALALGDIELVKPNLKELSALVNRDLTQPDDVRKAAQELVQSGKARRVVVSLGPQGALGIDSENCIQ
VVPPPVKSQSTVGAGDSMVGAMTLKLAQDASLEEMVRFGVAAGSAATLNQGTRLCSHDDTQKIYAYLSAQ

Specific function: Key control step of glycolysis. [C]

COG id: COG1105

COG function: function code G; Fructose-1-phosphate kinase and related fructose-6-phosphate kinase (PfkB)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the carbohydrate kinase pfkB family [H]

Homologues:

Organism=Escherichia coli, GI1788017, Length=308, Percent_Identity=92.5324675324675, Blast_Score=593, Evalue=1e-171,
Organism=Escherichia coli, GI1788493, Length=276, Percent_Identity=31.8840579710145, Blast_Score=102, Evalue=4e-23,

Paralogues:

None

Copy number: 496 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011611
- InterPro:   IPR002173
- InterPro:   IPR017583 [H]

Pfam domain/function: PF00294 PfkB [H]

EC number: =2.7.1.11 [H]

Molecular weight: Translated: 32546; Mature: 32546

Theoretical pI: Translated: 4.71; Mature: 4.71

Prosite motif: PS00583 PFKB_KINASES_1 ; PS00584 PFKB_KINASES_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVRIYTLTLAPSLDSATITPQIYPEGKLRCSAPVFEPGGGGINVARAIAHLGGTATAIFP
CEEEEEEEECCCCCCCEECCEECCCCCEEECCCCCCCCCCCHHHHHHHHHCCCCEEEEEE
AGGATGEHLVALLADENVPVSTVDAKDWTRQNLHVHVESSGEQYRFVMPGATLDDDEFRQ
CCCCCHHHEEEEEECCCCCEEEECCCCCCCCCEEEEEECCCCEEEEEECCCCCCHHHHHH
LEEQVLEIESGAILVISGSLPPGVKVEKLTQLISAAQKQGIRCIIDSSGDALTAALALGD
HHHHHHHHCCCEEEEEECCCCCCCCHHHHHHHHHHHHHCCCEEEEECCCCEEEEEEECCC
IELVKPNLKELSALVNRDLTQPDDVRKAAQELVQSGKARRVVVSLGPQGALGIDSENCIQ
CEEECCCHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCCCCCEE
VVPPPVKSQSTVGAGDSMVGAMTLKLAQDASLEEMVRFGVAAGSAATLNQGTRLCSHDDT
ECCCCCCCCCCCCCCCCHHHHEEEEHHCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCC
QKIYAYLSAQ
CEEEEEEECC
>Mature Secondary Structure
MVRIYTLTLAPSLDSATITPQIYPEGKLRCSAPVFEPGGGGINVARAIAHLGGTATAIFP
CEEEEEEEECCCCCCCEECCEECCCCCEEECCCCCCCCCCCHHHHHHHHHCCCCEEEEEE
AGGATGEHLVALLADENVPVSTVDAKDWTRQNLHVHVESSGEQYRFVMPGATLDDDEFRQ
CCCCCHHHEEEEEECCCCCEEEECCCCCCCCCEEEEEECCCCEEEEEECCCCCCHHHHHH
LEEQVLEIESGAILVISGSLPPGVKVEKLTQLISAAQKQGIRCIIDSSGDALTAALALGD
HHHHHHHHCCCEEEEEECCCCCCCCHHHHHHHHHHHHHCCCEEEEECCCCEEEEEEECCC
IELVKPNLKELSALVNRDLTQPDDVRKAAQELVQSGKARRVVVSLGPQGALGIDSENCIQ
CEEECCCHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCCCCCEE
VVPPPVKSQSTVGAGDSMVGAMTLKLAQDASLEEMVRFGVAAGSAATLNQGTRLCSHDDT
ECCCCCCCCCCCCCCCCHHHHEEEEHHCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCC
QKIYAYLSAQ
CEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 6235149; 9097039; 9278503; 6310120 [H]