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The map label for this gene is gdhA [H]

Identifier: 29141644

GI number: 29141644

Start: 1246514

End: 1247857

Strand: Reverse

Name: gdhA [H]

Synonym: t1178

Alternate gene names: 29141644

Gene position: 1247857-1246514 (Counterclockwise)

Preceding gene: 29141646

Following gene: 29141640

Centisome position: 26.04

GC content: 57.89

Gene sequence:

>1344_bases
ATGGATCAGACATGTTCTCTGGAATCGTTCCTCAACCATGTACAAAAGCGCGACCCGCATCAAACCGAGTTCGCGCAAGC
CGTCCGTGAAGTCATGACCACCCTGTGGCCGTTTCTTGAACAAAACCCCCGTTACCGCCACATGTCTTTACTGGAACGTT
TGGTTGAACCAGAGCGCGTAATCCAGTTTCGCGTAGTGTGGCTGGACGATAAAAATCAGGTGCAGGTAAATCGCGCCTGG
CGCGTGCAGTTCAACTCTGCTATCGGCCCCTATAAAGGCGGTATGCGCTTCCATCCTTCGGTAAACCTTTCCATCCTTAA
GTTCCTCGGCTTTGAGCAAACCTTTAAAAATGCCCTCACCACGTTGCCGATGGGCGGCGGTAAAGGCGGCAGCGATTTCG
ATCCGAAAGGGAAAAGCGAAGGCGAAGTGATGCGTTTCTGCCAGGCGCTGATGACTGAACTGTATCGCCATTTAGGGCCG
GATACCGACGTGCCTGCCGGGGATATCGGCGTGGGCGGGCGCGAAGTGGGCTTTATGGCGGGAATGATGCGCAAGCTTTC
CAATAACAGCGCCTGCGTCTTCACCGGCAAAGGGCTTTCCTTCGGCGGCAGCCTGATTCGTCCGGAGGCGACGGGCTATG
GCCTGGTCTACTTCACCGAAGCGATGCTTAAACGTCATGGTCTCGGTTTTGAAGGGATGCGCGTCGCCGTGTCCGGCTCC
GGCAACGTAGCGCAATACGCCATTGAGAAAGCGATGGCCTTTGGCGCGCGCGTGGTTACCGCGTCGGATTCGAGCGGGAC
GGTGGTCGATGAAAGTGGCTTCACGCCAGAGAAGCTGGCGCGGCTGTGTGAAATTAAAGCCAGCCGTGACGGTCGCGTCG
CTGACTACGCCCGTGAGTTTGGCTTAACCTATCTGGAAGGCCAGCAGCCCTGGAGCGTACCGGTGGATATCGCCCTGCCA
TGCGCGACGCAGAACGAACTGGACGTTGACGCCGCGCGCGTACTGATTGCCAATGGCGTGAAAGCCGTGGCGGAAGGGGC
AAATATGCCAACCACGATTGAAGCGACCGATCTCTTCCTTGAGGCGGGCGTACTGTTTGCGCCGGGTAAAGCCGCCAACG
CGGGCGGCGTGGCGACATCGGGGCTGGAAATGGCGCAGAACGCCGCGCGGTTGAGCTGGAAAGCCGAGAAAGTGGACGCC
CGTCTGCATCATATTATGCTGGATATCCACCACGCCTGTGTCAAGTACGGCGGCGACAATAAGCATACTAACTACGTTCA
GGGCGCCAACATCGCCGGTTTCGTGAAAGTCGCCGACGCCATGCTGGCGCAGGGCGTCATTTAA

Upstream 100 bases:

>100_bases
CTTACCGTCACATTGCTGATGGTATAGTCGAAAACTGCAAATACATATTCTGATAAAACGCAAATACAACCACATTAATA
TATAAGAGGTTTTTATATCT

Downstream 100 bases:

>100_bases
TCCCGTCGCCAGGCCAGATGGCTTTACGCTTATTCGGTCTGACGGCCTGTCTCCTCAGCAGGCGGGCTTTTTTTGCCCGC
TCGCTTACGCGGCGCGCTCT

Product: glutamate dehydrogenase

Products: NA

Alternate protein names: NADP-GDH [H]

Number of amino acids: Translated: 447; Mature: 447

Protein sequence:

>447_residues
MDQTCSLESFLNHVQKRDPHQTEFAQAVREVMTTLWPFLEQNPRYRHMSLLERLVEPERVIQFRVVWLDDKNQVQVNRAW
RVQFNSAIGPYKGGMRFHPSVNLSILKFLGFEQTFKNALTTLPMGGGKGGSDFDPKGKSEGEVMRFCQALMTELYRHLGP
DTDVPAGDIGVGGREVGFMAGMMRKLSNNSACVFTGKGLSFGGSLIRPEATGYGLVYFTEAMLKRHGLGFEGMRVAVSGS
GNVAQYAIEKAMAFGARVVTASDSSGTVVDESGFTPEKLARLCEIKASRDGRVADYAREFGLTYLEGQQPWSVPVDIALP
CATQNELDVDAARVLIANGVKAVAEGANMPTTIEATDLFLEAGVLFAPGKAANAGGVATSGLEMAQNAARLSWKAEKVDA
RLHHIMLDIHHACVKYGGDNKHTNYVQGANIAGFVKVADAMLAQGVI

Sequences:

>Translated_447_residues
MDQTCSLESFLNHVQKRDPHQTEFAQAVREVMTTLWPFLEQNPRYRHMSLLERLVEPERVIQFRVVWLDDKNQVQVNRAW
RVQFNSAIGPYKGGMRFHPSVNLSILKFLGFEQTFKNALTTLPMGGGKGGSDFDPKGKSEGEVMRFCQALMTELYRHLGP
DTDVPAGDIGVGGREVGFMAGMMRKLSNNSACVFTGKGLSFGGSLIRPEATGYGLVYFTEAMLKRHGLGFEGMRVAVSGS
GNVAQYAIEKAMAFGARVVTASDSSGTVVDESGFTPEKLARLCEIKASRDGRVADYAREFGLTYLEGQQPWSVPVDIALP
CATQNELDVDAARVLIANGVKAVAEGANMPTTIEATDLFLEAGVLFAPGKAANAGGVATSGLEMAQNAARLSWKAEKVDA
RLHHIMLDIHHACVKYGGDNKHTNYVQGANIAGFVKVADAMLAQGVI
>Mature_447_residues
MDQTCSLESFLNHVQKRDPHQTEFAQAVREVMTTLWPFLEQNPRYRHMSLLERLVEPERVIQFRVVWLDDKNQVQVNRAW
RVQFNSAIGPYKGGMRFHPSVNLSILKFLGFEQTFKNALTTLPMGGGKGGSDFDPKGKSEGEVMRFCQALMTELYRHLGP
DTDVPAGDIGVGGREVGFMAGMMRKLSNNSACVFTGKGLSFGGSLIRPEATGYGLVYFTEAMLKRHGLGFEGMRVAVSGS
GNVAQYAIEKAMAFGARVVTASDSSGTVVDESGFTPEKLARLCEIKASRDGRVADYAREFGLTYLEGQQPWSVPVDIALP
CATQNELDVDAARVLIANGVKAVAEGANMPTTIEATDLFLEAGVLFAPGKAANAGGVATSGLEMAQNAARLSWKAEKVDA
RLHHIMLDIHHACVKYGGDNKHTNYVQGANIAGFVKVADAMLAQGVI

Specific function: Unknown

COG id: COG0334

COG function: function code E; Glutamate dehydrogenase/leucine dehydrogenase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Glu/Leu/Phe/Val dehydrogenases family [H]

Homologues:

Organism=Homo sapiens, GI31377775, Length=397, Percent_Identity=30.4785894206549, Blast_Score=145, Evalue=9e-35,
Organism=Homo sapiens, GI4885281, Length=391, Percent_Identity=29.923273657289, Blast_Score=140, Evalue=4e-33,
Organism=Escherichia coli, GI1788059, Length=447, Percent_Identity=92.841163310962, Blast_Score=869, Evalue=0.0,
Organism=Caenorhabditis elegans, GI17544676, Length=342, Percent_Identity=30.1169590643275, Blast_Score=120, Evalue=1e-27,
Organism=Saccharomyces cerevisiae, GI6324951, Length=451, Percent_Identity=52.549889135255, Blast_Score=426, Evalue=1e-120,
Organism=Saccharomyces cerevisiae, GI6319256, Length=453, Percent_Identity=52.5386313465784, Blast_Score=421, Evalue=1e-118,
Organism=Drosophila melanogaster, GI24649283, Length=318, Percent_Identity=32.0754716981132, Blast_Score=136, Evalue=3e-32,
Organism=Drosophila melanogaster, GI45553475, Length=366, Percent_Identity=28.9617486338798, Blast_Score=125, Evalue=5e-29,
Organism=Drosophila melanogaster, GI45549226, Length=366, Percent_Identity=28.9617486338798, Blast_Score=125, Evalue=5e-29,
Organism=Drosophila melanogaster, GI24649460, Length=306, Percent_Identity=30.3921568627451, Blast_Score=107, Evalue=2e-23,

Paralogues:

None

Copy number: 140 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 9362 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006095
- InterPro:   IPR006096
- InterPro:   IPR006097
- InterPro:   IPR014362
- InterPro:   IPR016040 [H]

Pfam domain/function: PF00208 ELFV_dehydrog; PF02812 ELFV_dehydrog_N [H]

EC number: =1.4.1.4 [H]

Molecular weight: Translated: 48558; Mature: 48558

Theoretical pI: Translated: 7.09; Mature: 7.09

Prosite motif: PS00074 GLFV_DEHYDROGENASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDQTCSLESFLNHVQKRDPHQTEFAQAVREVMTTLWPFLEQNPRYRHMSLLERLVEPERV
CCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCE
IQFRVVWLDDKNQVQVNRAWRVQFNSAIGPYKGGMRFHPSVNLSILKFLGFEQTFKNALT
EEEEEEEECCCCEEEEEEEEEEEECCCCCCCCCCEEECCCCCHHHHHHHCHHHHHHHHHH
TLPMGGGKGGSDFDPKGKSEGEVMRFCQALMTELYRHLGPDTDVPAGDIGVGGREVGFMA
CCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHH
GMMRKLSNNSACVFTGKGLSFGGSLIRPEATGYGLVYFTEAMLKRHGLGFEGMRVAVSGS
HHHHHHCCCCEEEEECCCCCCCCCEECCCCCCCCHHHHHHHHHHHCCCCCCCEEEEEECC
GNVAQYAIEKAMAFGARVVTASDSSGTVVDESGFTPEKLARLCEIKASRDGRVADYAREF
CCHHHHHHHHHHHHCCEEEEECCCCCCEEECCCCCHHHHHHHHHHHCCCCCCHHHHHHHH
GLTYLEGQQPWSVPVDIALPCATQNELDVDAARVLIANGVKAVAEGANMPTTIEATDLFL
CCEEECCCCCCCCCEEEEECCCCCCCCCHHHHHHHHHCCHHHHHCCCCCCCEEHHHHHHH
EAGVLFAPGKAANAGGVATSGLEMAQNAARLSWKAEKVDARLHHIMLDIHHACVKYGGDN
HCCEEECCCCCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCC
KHTNYVQGANIAGFVKVADAMLAQGVI
CCCCEECCCCCHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MDQTCSLESFLNHVQKRDPHQTEFAQAVREVMTTLWPFLEQNPRYRHMSLLERLVEPERV
CCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCE
IQFRVVWLDDKNQVQVNRAWRVQFNSAIGPYKGGMRFHPSVNLSILKFLGFEQTFKNALT
EEEEEEEECCCCEEEEEEEEEEEECCCCCCCCCCEEECCCCCHHHHHHHCHHHHHHHHHH
TLPMGGGKGGSDFDPKGKSEGEVMRFCQALMTELYRHLGPDTDVPAGDIGVGGREVGFMA
CCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHH
GMMRKLSNNSACVFTGKGLSFGGSLIRPEATGYGLVYFTEAMLKRHGLGFEGMRVAVSGS
HHHHHHCCCCEEEEECCCCCCCCCEECCCCCCCCHHHHHHHHHHHCCCCCCCEEEEEECC
GNVAQYAIEKAMAFGARVVTASDSSGTVVDESGFTPEKLARLCEIKASRDGRVADYAREF
CCHHHHHHHHHHHHCCEEEEECCCCCCEEECCCCCHHHHHHHHHHHCCCCCCHHHHHHHH
GLTYLEGQQPWSVPVDIALPCATQNELDVDAARVLIANGVKAVAEGANMPTTIEATDLFL
CCEEECCCCCCCCCEEEEECCCCCCCCCHHHHHHHHHCCHHHHHCCCCCCCEEHHHHHHH
EAGVLFAPGKAANAGGVATSGLEMAQNAARLSWKAEKVDARLHHIMLDIHHACVKYGGDN
HCCEEECCCCCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCC
KHTNYVQGANIAGFVKVADAMLAQGVI
CCCCEECCCCCHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 6308576; 6373501; 9097039; 9278503; 9298646 [H]