Click here to switch to the map view.

The map label for this gene is sppA

Identifier: 29141640

GI number: 29141640

Start: 1240788

End: 1242644

Strand: Reverse

Name: sppA

Synonym: t1174

Alternate gene names: 29141640

Gene position: 1242644-1240788 (Counterclockwise)

Preceding gene: 29141644

Following gene: 29141639

Centisome position: 25.93

GC content: 55.84

Gene sequence:

>1857_bases
ATGCGAACCCTGTGGCGATTTATTGCCGGATTTTTTAAATGGACGTGGCGAGTGTTGAACTTCGTCCGTGAAATGGTACT
CAACCTGTTCTTTATTTTTCTGGTCCTGGTGGGCGTTGGGATCTGGATGCAGATCGGTAACGGCAGCAACAGTGAGCAAA
CGGCGCGCGGCGCTTTGCTGCTGGATATTTCCGGCGTCATTGTGGATAAACCCTCCACCAATCACCGTCTGGGCGCGCTG
GGACGCCAGTTATTTGGCGCCAGCTCCGACCGTCTGCAGGAAAACTCCCTGTTTGACATCGTCAACGCTATTCGTCAGGC
GAAAGATGACCGTAACATCACGGGTATCGTTCTGGATCTGAAAAACTTCACCGGTGCCGATCAGCCGTCAATGCGCTATA
TCGGTAAAGCGCTGCGCGAATTCCGCGATAGCGGCAAACCGGTTTTCGCCGTGGGTGAAAACTACAGCCAGGGTCAGTAT
TACCTCGCCAGTTTCGCCAATAAAATTTGGCTTTCCCCACAGGGTCAGGTAGATCTTCACGGCTTCGCTACGAATGGCCT
GTACTACAAAACGCTGCTGGATAAACTGAAAGTCTCTACCCACGTTTTCCGGGTCGGCACCTATAAATCCGCCGTCGAGC
CGTTTATCCGCGACGATATGTCGCCCGCCGCCCGCGAGGCCGACAGCCGCTGGATAGGCGAACTGTGGCAGAACTACCTG
CATACCGTTTCCGCCAATCGCCAGATTTCGCCGCAACAACTCTTCCCCGGCGCCCAGGCTATTATCGACGGGTTAACCAG
CGTGGGCGGTGACACCGCCAAATATGCGCTCGACCATAAACTGGTGGACGCCCTCGCCTCCAGCGCAGATGTTGAAAAAG
CGCTGACGAAGCAGTTTGGCTGGAGCAAAACCGAAAATAACTATCGCGCGATCAGCTATTACGATTATTCGCTGAAAACG
CCTGCGGATACCGGCGGTACTATTGCGGTTATTTTCGCCAATGGCGCGATTATGGATGGCGAAGAAACGCCAGGGAATGT
CGGCGGCGACACTACGGCATCGCAGATCCGCGACGCACGCCTTGATCCTAAAGTGAAAGCGATTGTGCTGCGCGTCAATA
GCCCAGGCGGTAGCGTCAACGCCTCCGAAGTTATCCGCGCCGAACTGGCGGCGGCAAGAGCGGCTGGCAAACCGGTGGTG
GTCTCAATGGGCGGCATGGCGGCCTCCGGCGGTTACTGGATCTCTACGCCGGCAAACTATATCGTGGCCAGCCCCAGCAC
GCTGACGGGTTCAATTGGCATCTTCGGCGTAATTAATACGGTAGAAAACAGCCTGTCGTCGATTGGCGTACACAGCGACG
GCGTTTCCACCTCGCCGCTGGCGGATATTTCGATGACTAAAGCGCTGTCACCGGAAGTGCAGCAGATGATGCAACTCAGT
ATTGAGTACGGCTACAAACGCTTTATCACGCTGGTGGCAGACGCGCGTAAGCGTACGCCGGAGCAGATTGACAAAATCGC
TCAAGGCCATGTCTGGACCGGAGAAGACGCGAAAGCCAATGGTCTGGTGGACAGTCTCGGCGACTTTGACGACGCCGTCG
CCAAAGCGGCGGAGCTGGCGAAACTGAAACAGTGGCATCTTGATTACTATCAGGACGAACCGACAGTCCTTGATATGGTC
ATGGACAGTATGACCGGATCTGTACGCGCCATGCTGCCGGAGGCCATTCAGGCGATGCTCCCGGCGCCGCTCGTTTCCGC
CGCCAACACGGTGAAGGCCGAGGGGGATAAACTGGCGGCATTTAACGATCCACAAAACCGTTATGCGTTCTGTTTGACTT
GCGCGAACGTGCGCTAA

Upstream 100 bases:

>100_bases
ATTCCTGCTGACAACTGCCTGATGGGTCATTACGATAGCCACATCTTAATGCTTTATCCTCACCAAGGTATAAGCGTTGT
TACTCAGATAGGGAGAATAC

Downstream 100 bases:

>100_bases
TGCTTCATCAATAGCCATACCGGATGTCGGCGTCTGCTCATAGCCGCCATCCGATAATCATTAATACGTCCTTCGCGACA
CGACTGAACATTATCATGCA

Product: protease 4

Products: NA

Alternate protein names: Endopeptidase IV; Protease IV; Signal peptide peptidase

Number of amino acids: Translated: 618; Mature: 618

Protein sequence:

>618_residues
MRTLWRFIAGFFKWTWRVLNFVREMVLNLFFIFLVLVGVGIWMQIGNGSNSEQTARGALLLDISGVIVDKPSTNHRLGAL
GRQLFGASSDRLQENSLFDIVNAIRQAKDDRNITGIVLDLKNFTGADQPSMRYIGKALREFRDSGKPVFAVGENYSQGQY
YLASFANKIWLSPQGQVDLHGFATNGLYYKTLLDKLKVSTHVFRVGTYKSAVEPFIRDDMSPAAREADSRWIGELWQNYL
HTVSANRQISPQQLFPGAQAIIDGLTSVGGDTAKYALDHKLVDALASSADVEKALTKQFGWSKTENNYRAISYYDYSLKT
PADTGGTIAVIFANGAIMDGEETPGNVGGDTTASQIRDARLDPKVKAIVLRVNSPGGSVNASEVIRAELAAARAAGKPVV
VSMGGMAASGGYWISTPANYIVASPSTLTGSIGIFGVINTVENSLSSIGVHSDGVSTSPLADISMTKALSPEVQQMMQLS
IEYGYKRFITLVADARKRTPEQIDKIAQGHVWTGEDAKANGLVDSLGDFDDAVAKAAELAKLKQWHLDYYQDEPTVLDMV
MDSMTGSVRAMLPEAIQAMLPAPLVSAANTVKAEGDKLAAFNDPQNRYAFCLTCANVR

Sequences:

>Translated_618_residues
MRTLWRFIAGFFKWTWRVLNFVREMVLNLFFIFLVLVGVGIWMQIGNGSNSEQTARGALLLDISGVIVDKPSTNHRLGAL
GRQLFGASSDRLQENSLFDIVNAIRQAKDDRNITGIVLDLKNFTGADQPSMRYIGKALREFRDSGKPVFAVGENYSQGQY
YLASFANKIWLSPQGQVDLHGFATNGLYYKTLLDKLKVSTHVFRVGTYKSAVEPFIRDDMSPAAREADSRWIGELWQNYL
HTVSANRQISPQQLFPGAQAIIDGLTSVGGDTAKYALDHKLVDALASSADVEKALTKQFGWSKTENNYRAISYYDYSLKT
PADTGGTIAVIFANGAIMDGEETPGNVGGDTTASQIRDARLDPKVKAIVLRVNSPGGSVNASEVIRAELAAARAAGKPVV
VSMGGMAASGGYWISTPANYIVASPSTLTGSIGIFGVINTVENSLSSIGVHSDGVSTSPLADISMTKALSPEVQQMMQLS
IEYGYKRFITLVADARKRTPEQIDKIAQGHVWTGEDAKANGLVDSLGDFDDAVAKAAELAKLKQWHLDYYQDEPTVLDMV
MDSMTGSVRAMLPEAIQAMLPAPLVSAANTVKAEGDKLAAFNDPQNRYAFCLTCANVR
>Mature_618_residues
MRTLWRFIAGFFKWTWRVLNFVREMVLNLFFIFLVLVGVGIWMQIGNGSNSEQTARGALLLDISGVIVDKPSTNHRLGAL
GRQLFGASSDRLQENSLFDIVNAIRQAKDDRNITGIVLDLKNFTGADQPSMRYIGKALREFRDSGKPVFAVGENYSQGQY
YLASFANKIWLSPQGQVDLHGFATNGLYYKTLLDKLKVSTHVFRVGTYKSAVEPFIRDDMSPAAREADSRWIGELWQNYL
HTVSANRQISPQQLFPGAQAIIDGLTSVGGDTAKYALDHKLVDALASSADVEKALTKQFGWSKTENNYRAISYYDYSLKT
PADTGGTIAVIFANGAIMDGEETPGNVGGDTTASQIRDARLDPKVKAIVLRVNSPGGSVNASEVIRAELAAARAAGKPVV
VSMGGMAASGGYWISTPANYIVASPSTLTGSIGIFGVINTVENSLSSIGVHSDGVSTSPLADISMTKALSPEVQQMMQLS
IEYGYKRFITLVADARKRTPEQIDKIAQGHVWTGEDAKANGLVDSLGDFDDAVAKAAELAKLKQWHLDYYQDEPTVLDMV
MDSMTGSVRAMLPEAIQAMLPAPLVSAANTVKAEGDKLAAFNDPQNRYAFCLTCANVR

Specific function: Digestion of the cleaved signal peptides. This activity is necessary to maintain proper secretion of mature proteins across the membrane

COG id: COG0616

COG function: function code OU; Periplasmic serine proteases (ClpP class)

Gene ontology:

Cell location: Cell inner membrane; Single-pass membrane protein

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S49 family

Homologues:

Organism=Escherichia coli, GI1788064, Length=618, Percent_Identity=86.2459546925566, Blast_Score=1098, Evalue=0.0,
Organism=Escherichia coli, GI1787527, Length=180, Percent_Identity=28.3333333333333, Blast_Score=72, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): SPPA_SALTI (Q8Z6F3)

Other databases:

- EMBL:   AL627271
- EMBL:   AE014613
- RefSeq:   NP_456217.1
- RefSeq:   NP_804982.1
- ProteinModelPortal:   Q8Z6F3
- SMR:   Q8Z6F3
- MEROPS:   S49.001
- GeneID:   1070592
- GeneID:   1248188
- GenomeReviews:   AE014613_GR
- GenomeReviews:   AL513382_GR
- KEGG:   stt:t1174
- KEGG:   sty:STY1819
- HOGENOM:   HBG686572
- OMA:   GGYWIAT
- ProtClustDB:   PRK10949
- BioCyc:   SENT209261:T1174-MONOMER
- BioCyc:   SENT220341:STY1819-MONOMER
- InterPro:   IPR004634
- InterPro:   IPR004635
- InterPro:   IPR002142
- PIRSF:   PIRSF001217
- TIGRFAMs:   TIGR00705
- TIGRFAMs:   TIGR00706

Pfam domain/function: PF01343 Peptidase_S49

EC number: 3.4.21.-

Molecular weight: Translated: 67190; Mature: 67190

Theoretical pI: Translated: 6.73; Mature: 6.73

Prosite motif: NA

Important sites: ACT_SITE 209-209 ACT_SITE 409-409

Signals:

None

Transmembrane regions:

HASH(0xd720c24)-;

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRTLWRFIAGFFKWTWRVLNFVREMVLNLFFIFLVLVGVGIWMQIGNGSNSEQTARGALL
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCCCCHHHCCEEE
LDISGVIVDKPSTNHRLGALGRQLFGASSDRLQENSLFDIVNAIRQAKDDRNITGIVLDL
EEECCEEEECCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEE
KNFTGADQPSMRYIGKALREFRDSGKPVFAVGENYSQGQYYLASFANKIWLSPQGQVDLH
CCCCCCCCCHHHHHHHHHHHHHHCCCEEEEECCCCCCCHHHHHHHHHHEECCCCCCEEEE
GFATNGLYYKTLLDKLKVSTHVFRVGTYKSAVEPFIRDDMSPAAREADSRWIGELWQNYL
EEEECCCHHHHHHHHHHHHHHEEEECCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHH
HTVSANRQISPQQLFPGAQAIIDGLTSVGGDTAKYALDHKLVDALASSADVEKALTKQFG
HHHCCCCCCCHHHHCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
WSKTENNYRAISYYDYSLKTPADTGGTIAVIFANGAIMDGEETPGNVGGDTTASQIRDAR
CCCCCCCEEEEEEEEEEECCCCCCCCEEEEEEECCEEECCCCCCCCCCCCCHHHHHHHCC
LDPKVKAIVLRVNSPGGSVNASEVIRAELAAARAAGKPVVVSMGGMAASGGYWISTPANY
CCCCEEEEEEEEECCCCCCCHHHHHHHHHHHHHCCCCCEEEEECCEEECCCEEEECCCCE
IVASPSTLTGSIGIFGVINTVENSLSSIGVHSDGVSTSPLADISMTKALSPEVQQMMQLS
EEECCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHCCHHHHHHHHHH
IEYGYKRFITLVADARKRTPEQIDKIAQGHVWTGEDAKANGLVDSLGDFDDAVAKAAELA
HHHHHHHHHHHHHHHHHCCHHHHHHHHCCCEECCCCCCCCCCHHHHCCHHHHHHHHHHHH
KLKQWHLDYYQDEPTVLDMVMDSMTGSVRAMLPEAIQAMLPAPLVSAANTVKAEGDKLAA
HHHHHHHHHCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHCCCHHHHHCHHHHCCCCEEEE
FNDPQNRYAFCLTCANVR
CCCCCCCEEEEEEECCCH
>Mature Secondary Structure
MRTLWRFIAGFFKWTWRVLNFVREMVLNLFFIFLVLVGVGIWMQIGNGSNSEQTARGALL
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCCCCHHHCCEEE
LDISGVIVDKPSTNHRLGALGRQLFGASSDRLQENSLFDIVNAIRQAKDDRNITGIVLDL
EEECCEEEECCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEE
KNFTGADQPSMRYIGKALREFRDSGKPVFAVGENYSQGQYYLASFANKIWLSPQGQVDLH
CCCCCCCCCHHHHHHHHHHHHHHCCCEEEEECCCCCCCHHHHHHHHHHEECCCCCCEEEE
GFATNGLYYKTLLDKLKVSTHVFRVGTYKSAVEPFIRDDMSPAAREADSRWIGELWQNYL
EEEECCCHHHHHHHHHHHHHHEEEECCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHH
HTVSANRQISPQQLFPGAQAIIDGLTSVGGDTAKYALDHKLVDALASSADVEKALTKQFG
HHHCCCCCCCHHHHCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
WSKTENNYRAISYYDYSLKTPADTGGTIAVIFANGAIMDGEETPGNVGGDTTASQIRDAR
CCCCCCCEEEEEEEEEEECCCCCCCCEEEEEEECCEEECCCCCCCCCCCCCHHHHHHHCC
LDPKVKAIVLRVNSPGGSVNASEVIRAELAAARAAGKPVVVSMGGMAASGGYWISTPANY
CCCCEEEEEEEEECCCCCCCHHHHHHHHHHHHHCCCCCEEEEECCEEECCCEEEECCCCE
IVASPSTLTGSIGIFGVINTVENSLSSIGVHSDGVSTSPLADISMTKALSPEVQQMMQLS
EEECCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHCCHHHHHHHHHH
IEYGYKRFITLVADARKRTPEQIDKIAQGHVWTGEDAKANGLVDSLGDFDDAVAKAAELA
HHHHHHHHHHHHHHHHHCCHHHHHHHHCCCEECCCCCCCCCCHHHHCCHHHHHHHHHHHH
KLKQWHLDYYQDEPTVLDMVMDSMTGSVRAMLPEAIQAMLPAPLVSAANTVKAEGDKLAA
HHHHHHHHHCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHCCCHHHHHCHHHHCCCCEEEE
FNDPQNRYAFCLTCANVR
CCCCCCCEEEEEEECCCH

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; Acting on peptide bonds (Peptidases) [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11677608; 12644504