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The map label for this gene is prfA [H]

Identifier: 29141572

GI number: 29141572

Start: 1185117

End: 1186199

Strand: Direct

Name: prfA [H]

Synonym: t1100

Alternate gene names: 29141572

Gene position: 1185117-1186199 (Clockwise)

Preceding gene: 29141571

Following gene: 29141573

Centisome position: 24.73

GC content: 57.99

Gene sequence:

>1083_bases
ATGAAGCCTTCTATCGTTGCCAAACTGGAAGCCCTGCACGAACGCCATGAGGAAGTTCAGGCGTTGCTGGGCGATGCGGG
AATTATCGCCGACCAGGACCGCTTTCGCGCATTGTCGCGCGAATATGCGCAATTAAGCGACGTTTCTCGCTGTTTTACGG
ACTGGCAACAGGTTCAGGACGATATCGAGACGGCTCAGATGATGCTCGACGATCCTGAAATGCGGGAAATGGCGCAGGAA
GAACTGCGCGAAGCGAAAGAAAAAAGCGAACAACTGGAGCAACAGTTACAGGTACTGCTGCTGCCGAAAGATCCGGACGA
TGAACGAAACGCGTTCCTTGAGGTTCGCGCCGGCACTGGCGGCGACGAAGCCGCGCTGTTTGCCGGCGATCTGTTCCGCA
TGTACAGCCGTTATGCCGAAGCGCGCCGCTGGCGCGTGGAGATCATGAGCATGAGCGAAGGCGAGCATGGCGGTTATAAA
GAGATCATCGCCAAAATCAGCGGCGACGGCGTGTATGGCCGACTGAAATTTGAGTCCGGCGGACACCGCGTACAGCGTGT
TCCGGCGACCGAGTCGCAGGGGCGTATCCATACCTCCGCCTGTACCGTCGCCGTGATGCCGGAGCTGCCGGAAGCCGAGC
TGCCGGATATTAACCCGGCGGATCTGCGCATTGATACGTTTCGTTCTTCCGGCGCGGGCGGTCAGCACGTTAACACCACC
GACTCCGCTATCCGTATTACCCACTTGCCGACCGGCATCGTGGTGGAATGCCAGGACGAGCGTTCGCAGCATAAAAACAA
AGCGAAAGCGCTCTCGGTGCTCGGGGCGCGCATTCACGCCGCCGAAACGGCAAAACGCCAGCAGGCCGAGGCGTCAACGC
GCCGCAACCTGCTTGGCAGCGGCGATCGCAGCGATCGTAACCGGACCTATAATTTCCCGCAGGGGCGCGTGACCGATCAT
CGTATTAATCTGACGTTATATCGCCTTGATGAAACGATGGAAGGTAAGCTGGATATGCTGATTGAGCCGATTGTTCAGGA
ACACCAGGCTGACCTGTTAGCCGCGTTGTCCGAGCAGGAATAA

Upstream 100 bases:

>100_bases
GCCCGTGACGGGGATGACGAACGCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAGCAGCACACACCACACTTTTT
TACAGGGTGAATTTACGCCT

Downstream 100 bases:

>100_bases
TGGATTTTCAGCACTGGCTACATGAGGCGGTAAACCAGCTCCGGGACAGCGACAGCCCCCGGCGCGACGCCGAGATCCTG
CTTGAGTACGTTACGGGCAA

Product: peptide chain release factor 1

Products: NA

Alternate protein names: RF-1 [H]

Number of amino acids: Translated: 360; Mature: 360

Protein sequence:

>360_residues
MKPSIVAKLEALHERHEEVQALLGDAGIIADQDRFRALSREYAQLSDVSRCFTDWQQVQDDIETAQMMLDDPEMREMAQE
ELREAKEKSEQLEQQLQVLLLPKDPDDERNAFLEVRAGTGGDEAALFAGDLFRMYSRYAEARRWRVEIMSMSEGEHGGYK
EIIAKISGDGVYGRLKFESGGHRVQRVPATESQGRIHTSACTVAVMPELPEAELPDINPADLRIDTFRSSGAGGQHVNTT
DSAIRITHLPTGIVVECQDERSQHKNKAKALSVLGARIHAAETAKRQQAEASTRRNLLGSGDRSDRNRTYNFPQGRVTDH
RINLTLYRLDETMEGKLDMLIEPIVQEHQADLLAALSEQE

Sequences:

>Translated_360_residues
MKPSIVAKLEALHERHEEVQALLGDAGIIADQDRFRALSREYAQLSDVSRCFTDWQQVQDDIETAQMMLDDPEMREMAQE
ELREAKEKSEQLEQQLQVLLLPKDPDDERNAFLEVRAGTGGDEAALFAGDLFRMYSRYAEARRWRVEIMSMSEGEHGGYK
EIIAKISGDGVYGRLKFESGGHRVQRVPATESQGRIHTSACTVAVMPELPEAELPDINPADLRIDTFRSSGAGGQHVNTT
DSAIRITHLPTGIVVECQDERSQHKNKAKALSVLGARIHAAETAKRQQAEASTRRNLLGSGDRSDRNRTYNFPQGRVTDH
RINLTLYRLDETMEGKLDMLIEPIVQEHQADLLAALSEQE
>Mature_360_residues
MKPSIVAKLEALHERHEEVQALLGDAGIIADQDRFRALSREYAQLSDVSRCFTDWQQVQDDIETAQMMLDDPEMREMAQE
ELREAKEKSEQLEQQLQVLLLPKDPDDERNAFLEVRAGTGGDEAALFAGDLFRMYSRYAEARRWRVEIMSMSEGEHGGYK
EIIAKISGDGVYGRLKFESGGHRVQRVPATESQGRIHTSACTVAVMPELPEAELPDINPADLRIDTFRSSGAGGQHVNTT
DSAIRITHLPTGIVVECQDERSQHKNKAKALSVLGARIHAAETAKRQQAEASTRRNLLGSGDRSDRNRTYNFPQGRVTDH
RINLTLYRLDETMEGKLDMLIEPIVQEHQADLLAALSEQE

Specific function: Peptide chain release factor 1 directs the termination of translation in response to the peptide chain termination codons UAG and UAA [H]

COG id: COG0216

COG function: function code J; Protein chain release factor A

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic/mitochondrial release factor family [H]

Homologues:

Organism=Homo sapiens, GI166795303, Length=289, Percent_Identity=50.1730103806228, Blast_Score=273, Evalue=3e-73,
Organism=Homo sapiens, GI34577120, Length=342, Percent_Identity=39.766081871345, Blast_Score=243, Evalue=2e-64,
Organism=Homo sapiens, GI166795305, Length=190, Percent_Identity=50.5263157894737, Blast_Score=189, Evalue=3e-48,
Organism=Escherichia coli, GI1787462, Length=360, Percent_Identity=96.9444444444444, Blast_Score=692, Evalue=0.0,
Organism=Escherichia coli, GI2367172, Length=338, Percent_Identity=38.7573964497041, Blast_Score=207, Evalue=9e-55,
Organism=Caenorhabditis elegans, GI17542784, Length=326, Percent_Identity=33.1288343558282, Blast_Score=182, Evalue=2e-46,
Organism=Saccharomyces cerevisiae, GI6321295, Length=313, Percent_Identity=43.1309904153355, Blast_Score=258, Evalue=1e-69,
Organism=Drosophila melanogaster, GI19921226, Length=327, Percent_Identity=39.1437308868502, Blast_Score=202, Evalue=4e-52,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005139
- InterPro:   IPR000352
- InterPro:   IPR004373 [H]

Pfam domain/function: PF03462 PCRF; PF00472 RF-1 [H]

EC number: NA

Molecular weight: Translated: 40462; Mature: 40462

Theoretical pI: Translated: 4.91; Mature: 4.91

Prosite motif: PS00745 RF_PROK_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKPSIVAKLEALHERHEEVQALLGDAGIIADQDRFRALSREYAQLSDVSRCFTDWQQVQD
CCCHHHHHHHHHHHHHHHHHHHHCCCCCEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DIETAQMMLDDPEMREMAQEELREAKEKSEQLEQQLQVLLLPKDPDDERNAFLEVRAGTG
HHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCCCCCCCEEEEEECCCC
GDEAALFAGDLFRMYSRYAEARRWRVEIMSMSEGEHGGYKEIIAKISGDGVYGRLKFESG
CCCHHHHHHHHHHHHHHHHHHHHHEEEEEECCCCCCCCHHHHHHHHCCCCEEEEEEECCC
GHRVQRVPATESQGRIHTSACTVAVMPELPEAELPDINPADLRIDTFRSSGAGGQHVNTT
CCEEEECCCCCCCCEEEECEEEEEECCCCCCCCCCCCCCCCEEEEECCCCCCCCCCCCCC
DSAIRITHLPTGIVVECQDERSQHKNKAKALSVLGARIHAAETAKRQQAEASTRRNLLGS
CCEEEEEECCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
GDRSDRNRTYNFPQGRVTDHRINLTLYRLDETMEGKLDMLIEPIVQEHQADLLAALSEQE
CCCCCCCCCCCCCCCCCCCEEEEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MKPSIVAKLEALHERHEEVQALLGDAGIIADQDRFRALSREYAQLSDVSRCFTDWQQVQD
CCCHHHHHHHHHHHHHHHHHHHHCCCCCEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DIETAQMMLDDPEMREMAQEELREAKEKSEQLEQQLQVLLLPKDPDDERNAFLEVRAGTG
HHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCCCCCCCEEEEEECCCC
GDEAALFAGDLFRMYSRYAEARRWRVEIMSMSEGEHGGYKEIIAKISGDGVYGRLKFESG
CCCHHHHHHHHHHHHHHHHHHHHHEEEEEECCCCCCCCHHHHHHHHCCCCEEEEEEECCC
GHRVQRVPATESQGRIHTSACTVAVMPELPEAELPDINPADLRIDTFRSSGAGGQHVNTT
CCEEEECCCCCCCCEEEECEEEEEECCCCCCCCCCCCCCCCEEEEECCCCCCCCCCCCCC
DSAIRITHLPTGIVVECQDERSQHKNKAKALSVLGARIHAAETAKRQQAEASTRRNLLGS
CCEEEEEECCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
GDRSDRNRTYNFPQGRVTDHRINLTLYRLDETMEGKLDMLIEPIVQEHQADLLAALSEQE
CCCCCCCCCCCCCCCCCCCEEEEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA