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The map label for this gene is hemK [H]

Identifier: 29141573

GI number: 29141573

Start: 1186199

End: 1187032

Strand: Direct

Name: hemK [H]

Synonym: t1101

Alternate gene names: 29141573

Gene position: 1186199-1187032 (Clockwise)

Preceding gene: 29141572

Following gene: 29141574

Centisome position: 24.75

GC content: 58.75

Gene sequence:

>834_bases
ATGGATTTTCAGCACTGGCTACATGAGGCGGTAAACCAGCTCCGGGACAGCGACAGCCCCCGGCGCGACGCCGAGATCCT
GCTTGAGTACGTTACGGGCAAGAGGCGGACGTATATCATGGCCTTTGGCGAAACGCCGCTTACCGATGTCCAGCAACAAC
AGCTCGCGGAGCTGCTGCAGCGGCGTAAACAGGGCGAACCCATTGCGTACCTGACGGGCTTACGCGAATTTTGGTCGCTG
CCGCTCTTCGTTTCTCCCGCCACGCTAATCCCGCGCCCGGATACCGAATGTCTGGTTGAACAGGCGTTGGCGCGATTGCC
GGTCAAAACCTGTCGTATTCTGGATTTAGGCACCGGAACCGGCGCGATTGCGCTGGCGCTGGCCTGCGAGCGACCGGATT
GCGAGGTGACTGCGGTCGATCGTATGCCTGATGCGGTTGCGCTGGCGATACGCAACGCGGAACATTTGGCTATCCGGAAT
GTGCGTATACTGCAAAGTTGTTGGTTCAGCGCACTATCGGGGCAGCAGTTCGACATGATTGTCAGCAATCCGCCCTACAT
TGACGCGCAGGACCCGCACCTTAGCGAAGGCGATGTACGCTTTGAACCGCGCTCAGCGTTGGTGGCGGACGAAAACGGTA
TGGCGGATCTTACGCATATTATCGATAATGCCCGCCAGATGTTAACGCCCGGCGGCTTCCTGCTGCTGGAACACGGTTGG
CGGCAGGGCGAAGCGGTAAGAGCGGCTTTCAGGCGGTCTGGTTACACGGATGTAGAAACGTGTCGTGATTATGGTGGCAA
TGAACGCGTGGCCTGCGGGCGTTTTACGCCATGA

Upstream 100 bases:

>100_bases
GCCTTGATGAAACGATGGAAGGTAAGCTGGATATGCTGATTGAGCCGATTGTTCAGGAACACCAGGCTGACCTGTTAGCC
GCGTTGTCCGAGCAGGAATA

Downstream 100 bases:

>100_bases
CAATTGCAATGTTGCTCACGCTTCATCTTATTTGTGTTGCGCTCTCCGTCAGTTTGTTTGTCGCTCGTTACTGGTGGCGC
TATTGCGGCCATGCGTTGGC

Product: N5-glutamine S-adenosyl-L-methionine-dependent methyltransferase

Products: NA

Alternate protein names: M.StyLTHemKP; Protein-(glutamine-N(5)) MTase hemK; Protein-glutamine N-methyltransferase hemK [H]

Number of amino acids: Translated: 277; Mature: 277

Protein sequence:

>277_residues
MDFQHWLHEAVNQLRDSDSPRRDAEILLEYVTGKRRTYIMAFGETPLTDVQQQQLAELLQRRKQGEPIAYLTGLREFWSL
PLFVSPATLIPRPDTECLVEQALARLPVKTCRILDLGTGTGAIALALACERPDCEVTAVDRMPDAVALAIRNAEHLAIRN
VRILQSCWFSALSGQQFDMIVSNPPYIDAQDPHLSEGDVRFEPRSALVADENGMADLTHIIDNARQMLTPGGFLLLEHGW
RQGEAVRAAFRRSGYTDVETCRDYGGNERVACGRFTP

Sequences:

>Translated_277_residues
MDFQHWLHEAVNQLRDSDSPRRDAEILLEYVTGKRRTYIMAFGETPLTDVQQQQLAELLQRRKQGEPIAYLTGLREFWSL
PLFVSPATLIPRPDTECLVEQALARLPVKTCRILDLGTGTGAIALALACERPDCEVTAVDRMPDAVALAIRNAEHLAIRN
VRILQSCWFSALSGQQFDMIVSNPPYIDAQDPHLSEGDVRFEPRSALVADENGMADLTHIIDNARQMLTPGGFLLLEHGW
RQGEAVRAAFRRSGYTDVETCRDYGGNERVACGRFTP
>Mature_277_residues
MDFQHWLHEAVNQLRDSDSPRRDAEILLEYVTGKRRTYIMAFGETPLTDVQQQQLAELLQRRKQGEPIAYLTGLREFWSL
PLFVSPATLIPRPDTECLVEQALARLPVKTCRILDLGTGTGAIALALACERPDCEVTAVDRMPDAVALAIRNAEHLAIRN
VRILQSCWFSALSGQQFDMIVSNPPYIDAQDPHLSEGDVRFEPRSALVADENGMADLTHIIDNARQMLTPGGFLLLEHGW
RQGEAVRAAFRRSGYTDVETCRDYGGNERVACGRFTP

Specific function: Methylates the translation termination release factor RF1 on 'Gln-235' and RF2 on 'Gln-252' [H]

COG id: COG2890

COG function: function code J; Methylase of polypeptide chain release factors

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hemK family [H]

Homologues:

Organism=Homo sapiens, GI7705409, Length=286, Percent_Identity=31.4685314685315, Blast_Score=100, Evalue=2e-21,
Organism=Escherichia coli, GI1787463, Length=275, Percent_Identity=76.3636363636364, Blast_Score=438, Evalue=1e-124,
Organism=Escherichia coli, GI87082085, Length=196, Percent_Identity=37.2448979591837, Blast_Score=109, Evalue=2e-25,
Organism=Saccharomyces cerevisiae, GI6324266, Length=180, Percent_Identity=31.1111111111111, Blast_Score=67, Evalue=3e-12,
Organism=Drosophila melanogaster, GI24582226, Length=208, Percent_Identity=28.3653846153846, Blast_Score=84, Evalue=1e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002052
- InterPro:   IPR004556
- InterPro:   IPR019874
- InterPro:   IPR007848 [H]

Pfam domain/function: PF05175 MTS [H]

EC number: 2.1.1.-

Molecular weight: Translated: 31064; Mature: 31064

Theoretical pI: Translated: 5.05; Mature: 5.05

Prosite motif: PS00092 N6_MTASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDFQHWLHEAVNQLRDSDSPRRDAEILLEYVTGKRRTYIMAFGETPLTDVQQQQLAELLQ
CCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCEEEEEEECCCCCCHHHHHHHHHHHH
RRKQGEPIAYLTGLREFWSLPLFVSPATLIPRPDTECLVEQALARLPVKTCRILDLGTGT
HHHCCCCHHHHHHHHHHHCCCEEECCCCCCCCCCHHHHHHHHHHHCCHHHEEEEECCCCC
GAIALALACERPDCEVTAVDRMPDAVALAIRNAEHLAIRNVRILQSCWFSALSGQQFDMI
CCEEEEEECCCCCCCEEECCCCCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCEEEE
VSNPPYIDAQDPHLSEGDVRFEPRSALVADENGMADLTHIIDNARQMLTPGGFLLLEHGW
ECCCCCCCCCCCCCCCCCEEECCCCEEEECCCCHHHHHHHHHHHHHHHCCCCEEEEECCC
RQGEAVRAAFRRSGYTDVETCRDYGGNERVACGRFTP
CCCHHHHHHHHHCCCCHHHHHHHCCCCCCEECCCCCC
>Mature Secondary Structure
MDFQHWLHEAVNQLRDSDSPRRDAEILLEYVTGKRRTYIMAFGETPLTDVQQQQLAELLQ
CCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCEEEEEEECCCCCCHHHHHHHHHHHH
RRKQGEPIAYLTGLREFWSLPLFVSPATLIPRPDTECLVEQALARLPVKTCRILDLGTGT
HHHCCCCHHHHHHHHHHHCCCEEECCCCCCCCCCHHHHHHHHHHHCCHHHEEEEECCCCC
GAIALALACERPDCEVTAVDRMPDAVALAIRNAEHLAIRNVRILQSCWFSALSGQQFDMI
CCEEEEEECCCCCCCEEECCCCCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCEEEE
VSNPPYIDAQDPHLSEGDVRFEPRSALVADENGMADLTHIIDNARQMLTPGGFLLLEHGW
ECCCCCCCCCCCCCCCCCEEECCCCEEEECCCCHHHHHHHHHHHHHHHCCCCEEEEECCC
RQGEAVRAAFRRSGYTDVETCRDYGGNERVACGRFTP
CCCHHHHHHHHHCCCCHHHHHHHCCCCCCEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11677609; 2544564 [H]