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The map label for this gene is hyaF [H]

Identifier: 29141558

GI number: 29141558

Start: 1169212

End: 1170060

Strand: Reverse

Name: hyaF [H]

Synonym: t1085

Alternate gene names: 29141558

Gene position: 1170060-1169212 (Counterclockwise)

Preceding gene: 29141559

Following gene: 29141557

Centisome position: 24.42

GC content: 59.95

Gene sequence:

>849_bases
ATGAGCAACGCCTTTTTTCATCTGCTGGGGCCAGGCACGCAGCCTGATGACGCCAGTTTCTCGATGAATCCCCTACCGCT
CACCTGTCAGGTCAATGGCGACCCAAGTATGGCGGCGCTGGAACGCTGCGCTCACAGCCCGGCGGTGATGGCATTGTTGA
CCGATCTGCGAGGTCAGCTTGCCCGGCGCATCCCGGAAGTCGGCGACGTGCTGGGATGGGAGTTATCTCCCCTGAATGCC
GATGATCTCTCATTCCTCAATACGCTATTGGGCGAAGGCGAAGTCTCGGTACGCATTCAGCATCCGGACGGGAGTGAAAG
CGAGATCCAGGAGACCATCTTCTGCGGCCTGTGGCGGGTACGCCATCTGCATAACCGACGTCTGCTGACGGATCGTCTGG
AAGCGGGTAGCGCGCCCCTGACATTGTGGCAAGCGGCGACTGCCGATACGCTGCCTGACGACTCTCTGCTGCCTCCGCCT
GTCGCTGGCCTGATGAATGGACTGCCGCTGGCCCATGAGTTATTGGCGCACGTACGCGATCCGGCGTTGCAACCTCACAG
CATCAATTTGACCCAACTGCCGCTCAGCGAGGCCGATCGTCTTTTTCTGGCGCGTTTATGCGGACACGGGAACATCCAGA
TTCGCATCTCCGGGTATGGCGAAAGCCAAATCAACGCCACAGCATTGCGCCATCTCTGGCATGTGCGCTGTCTGGACGCC
CTTAAAGGGCCGTTGCTCGATAGCTATGAGATTTGTCCTTTGCCGGAGCTGGTACTGGCGGCCCCGGAAGATCTGGCCGA
TTCGCGCCAGCGCCTGGATGAAGTCTGCCGATGGCTGGAGACGCGCTAG

Upstream 100 bases:

>100_bases
GCGAGCTACGCGGCGCGCTCAGCGGCATTCATCCCTGGGCTGAACTGCTCACGCTGATGCGTTCGATGGTTGACACTCCC
GCAGCGCAGGAGACAGCACA

Downstream 100 bases:

>100_bases
CGCTCTCAACAGGAACGTCACCGGTGTCCGGTATTGATTAGCTTTTTACTCTCTTTCTGCCTACAGGAGTTAGCATGTGG
GATGTCATTGACTTATCGCG

Product: hydrogenase-1 operon protein HyaF

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 282; Mature: 281

Protein sequence:

>282_residues
MSNAFFHLLGPGTQPDDASFSMNPLPLTCQVNGDPSMAALERCAHSPAVMALLTDLRGQLARRIPEVGDVLGWELSPLNA
DDLSFLNTLLGEGEVSVRIQHPDGSESEIQETIFCGLWRVRHLHNRRLLTDRLEAGSAPLTLWQAATADTLPDDSLLPPP
VAGLMNGLPLAHELLAHVRDPALQPHSINLTQLPLSEADRLFLARLCGHGNIQIRISGYGESQINATALRHLWHVRCLDA
LKGPLLDSYEICPLPELVLAAPEDLADSRQRLDEVCRWLETR

Sequences:

>Translated_282_residues
MSNAFFHLLGPGTQPDDASFSMNPLPLTCQVNGDPSMAALERCAHSPAVMALLTDLRGQLARRIPEVGDVLGWELSPLNA
DDLSFLNTLLGEGEVSVRIQHPDGSESEIQETIFCGLWRVRHLHNRRLLTDRLEAGSAPLTLWQAATADTLPDDSLLPPP
VAGLMNGLPLAHELLAHVRDPALQPHSINLTQLPLSEADRLFLARLCGHGNIQIRISGYGESQINATALRHLWHVRCLDA
LKGPLLDSYEICPLPELVLAAPEDLADSRQRLDEVCRWLETR
>Mature_281_residues
SNAFFHLLGPGTQPDDASFSMNPLPLTCQVNGDPSMAALERCAHSPAVMALLTDLRGQLARRIPEVGDVLGWELSPLNAD
DLSFLNTLLGEGEVSVRIQHPDGSESEIQETIFCGLWRVRHLHNRRLLTDRLEAGSAPLTLWQAATADTLPDDSLLPPPV
AGLMNGLPLAHELLAHVRDPALQPHSINLTQLPLSEADRLFLARLCGHGNIQIRISGYGESQINATALRHLWHVRCLDAL
KGPLLDSYEICPLPELVLAAPEDLADSRQRLDEVCRWLETR

Specific function: Not known. Could enhance the incorporation of nickel to the hydrogenase [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hupH/hyaF family [H]

Homologues:

Organism=Escherichia coli, GI1787211, Length=279, Percent_Identity=75.2688172043011, Blast_Score=429, Evalue=1e-121,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006894 [H]

Pfam domain/function: PF04809 HupH_C [H]

EC number: NA

Molecular weight: Translated: 31001; Mature: 30870

Theoretical pI: Translated: 4.88; Mature: 4.88

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSNAFFHLLGPGTQPDDASFSMNPLPLTCQVNGDPSMAALERCAHSPAVMALLTDLRGQL
CCCCEEEEECCCCCCCCCCCCCCCCEEEEEECCCCCHHHHHHHCCCHHHHHHHHHHHHHH
ARRIPEVGDVLGWELSPLNADDLSFLNTLLGEGEVSVRIQHPDGSESEIQETIFCGLWRV
HHHCCHHHHHHCCCCCCCCCHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHHHHHHH
RHLHNRRLLTDRLEAGSAPLTLWQAATADTLPDDSLLPPPVAGLMNGLPLAHELLAHVRD
HHHHCCHHHHHHHHCCCCCEEEEHHCCCCCCCCCCCCCCCHHHHHCCCCHHHHHHHHHHC
PALQPHSINLTQLPLSEADRLFLARLCGHGNIQIRISGYGESQINATALRHLWHVRCLDA
CCCCCCCCCEEECCHHHHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHH
LKGPLLDSYEICPLPELVLAAPEDLADSRQRLDEVCRWLETR
HCCCCCCCCCCCCCCHHHHCCCHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SNAFFHLLGPGTQPDDASFSMNPLPLTCQVNGDPSMAALERCAHSPAVMALLTDLRGQL
CCCEEEEECCCCCCCCCCCCCCCCEEEEEECCCCCHHHHHHHCCCHHHHHHHHHHHHHH
ARRIPEVGDVLGWELSPLNADDLSFLNTLLGEGEVSVRIQHPDGSESEIQETIFCGLWRV
HHHCCHHHHHHCCCCCCCCCHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHHHHHHH
RHLHNRRLLTDRLEAGSAPLTLWQAATADTLPDDSLLPPPVAGLMNGLPLAHELLAHVRD
HHHHCCHHHHHHHHCCCCCEEEEHHCCCCCCCCCCCCCCCHHHHHCCCCHHHHHHHHHHC
PALQPHSINLTQLPLSEADRLFLARLCGHGNIQIRISGYGESQINATALRHLWHVRCLDA
CCCCCCCCCEEECCHHHHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHH
LKGPLLDSYEICPLPELVLAAPEDLADSRQRLDEVCRWLETR
HCCCCCCCCCCCCCCHHHHCCCHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2180913; 8905232; 9278503 [H]