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The map label for this gene is dcm [H]

Identifier: 29141374

GI number: 29141374

Start: 987120

End: 988550

Strand: Direct

Name: dcm [H]

Synonym: t0886

Alternate gene names: 29141374

Gene position: 987120-988550 (Clockwise)

Preceding gene: 29141373

Following gene: 29141375

Centisome position: 20.6

GC content: 54.09

Gene sequence:

>1431_bases
ATGCAGGAAAATATTTCAGTAACACACGCCCGGAACCTCATCGCCGACGACGCCGGAAGCGAGATCCAGGCGATGCTGAG
TCAATTGCTGGAAATCTACGATGTTAAAACGCTGGTGGCGCACCTTAACGGCCTGGGCGAACAGCACTGGAGCCCGGCCA
TCTTCAAGCGCGTAATGATGAACGCGGCATGGCATCGTTTGAGCGACAATGAACTCAGCTGTCTTAAAACAGAGTTGCCG
ACGCCGCCAGCGCATCATCCACATTACGCCTTTCGTTTTATCGATCTCTTCGCGGGCATCGGCGGCATTCGCCGCGGATT
TGAAGCGATAGGCGGACAGTGCGTGTTTACCAGCGAATGGAATAAGCACGCGGTACGGACATATAAAGCGAACTATTTTT
GCGATCCGCTGCAACATCGCTTTAATGAAGATATCCGCGATATCACATTGAGCCACCGGGAAGGGGTCAGCGATGATGAG
GCGGCGGAACACATTCGCCAGCATATTCCGCAACATGATGTCCTGCTGGCGGGCTTTCCCTGTCAGCCATTTTCTCTGGC
GGGCGTTTCCAAGAAAAATGCGCTGGGCCGCGCCCACGGCTTTGCCTGCGAGACTCAGGGGACGTTATTTTTTGATGTCG
TAAGAATTATCGACGCCCGCCGCCCCGCGCTGTTTGTGCTGGAAAACGTGAAAAACCTTAAAAGTCACGACCAGGGCAAC
ACCTTCCGCATTATTATGCAAACGCTCGATGAACTGGGATATGACGTGGCGGATGCCGCTGACAATGGCCCGGACGATCC
GAAAATTATCGACGGGCAGCACTTTCTTCCTCAGCATCGGGAACGTATTGTGTTGGTGGGATTCCGTCGCGATTTAAACC
TGAAAACCGATTTTACGTTACGCAATATCGCCCGTTGTTATCCACCGCGCCGTCCGACGCTGGCAGAACTGCTGGAGCCC
GTCGTCGAAGCCAAATATATCCTGACGCCGGTGCTGTGGAAATATTTATATCGCTACGCGAAAAAGCACCAGGCGCGGGG
AAACGGTTTTGGCTATGGCATGGTTTATCCTGACAATCCGGAAAGTGTGGCGCGCACGTTATCTGCTCGCTACTACAAAG
ATGGCGCCGAAATTCTGATCGATCGTGGTTGGGATATGGCGAAAGGCGAAGTGAATTTCGACGATGCTGGCAACCAACAA
CATCGTCCCCGCCGACTCACGCCGAGAGAGTGCGCGCGTTTAATGGGATTTGAGGCGCCGCAAACGTACCAGTTCAGGAT
ACCTGTCTCGGATACGCAGGCCTATCGCCAGTTTGGCAACTCCGTGGTGGTGCCGGTATTTGCCGCGGTAGCAAAGCTGC
TGGAACCCAAAATTCACCAGGCGGTGACGCTGCGTCAGAGAGAGACGGCAGATGGCGGACGTTCACGATAA

Upstream 100 bases:

>100_bases
ATCCAGATGTTTGCTACGTATCAGTAACCCCTGCCGCTGAAACGTAAACCGCGTCATTTTATGTTAGTCTGTCGGCAATT
ATTTTTGGCCGGTTAAATGT

Downstream 100 bases:

>100_bases
AGCCACGCGCAGTAAAAATATGCGCGCGATAGCGACCCGCGATACGGCGATTGAAAAACGCCTTGCTGGCCTGTTAAGCG
CGCAGGGGATTACGTTTCAT

Product: DNA cytosine methylase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 476; Mature: 476

Protein sequence:

>476_residues
MQENISVTHARNLIADDAGSEIQAMLSQLLEIYDVKTLVAHLNGLGEQHWSPAIFKRVMMNAAWHRLSDNELSCLKTELP
TPPAHHPHYAFRFIDLFAGIGGIRRGFEAIGGQCVFTSEWNKHAVRTYKANYFCDPLQHRFNEDIRDITLSHREGVSDDE
AAEHIRQHIPQHDVLLAGFPCQPFSLAGVSKKNALGRAHGFACETQGTLFFDVVRIIDARRPALFVLENVKNLKSHDQGN
TFRIIMQTLDELGYDVADAADNGPDDPKIIDGQHFLPQHRERIVLVGFRRDLNLKTDFTLRNIARCYPPRRPTLAELLEP
VVEAKYILTPVLWKYLYRYAKKHQARGNGFGYGMVYPDNPESVARTLSARYYKDGAEILIDRGWDMAKGEVNFDDAGNQQ
HRPRRLTPRECARLMGFEAPQTYQFRIPVSDTQAYRQFGNSVVVPVFAAVAKLLEPKIHQAVTLRQRETADGGRSR

Sequences:

>Translated_476_residues
MQENISVTHARNLIADDAGSEIQAMLSQLLEIYDVKTLVAHLNGLGEQHWSPAIFKRVMMNAAWHRLSDNELSCLKTELP
TPPAHHPHYAFRFIDLFAGIGGIRRGFEAIGGQCVFTSEWNKHAVRTYKANYFCDPLQHRFNEDIRDITLSHREGVSDDE
AAEHIRQHIPQHDVLLAGFPCQPFSLAGVSKKNALGRAHGFACETQGTLFFDVVRIIDARRPALFVLENVKNLKSHDQGN
TFRIIMQTLDELGYDVADAADNGPDDPKIIDGQHFLPQHRERIVLVGFRRDLNLKTDFTLRNIARCYPPRRPTLAELLEP
VVEAKYILTPVLWKYLYRYAKKHQARGNGFGYGMVYPDNPESVARTLSARYYKDGAEILIDRGWDMAKGEVNFDDAGNQQ
HRPRRLTPRECARLMGFEAPQTYQFRIPVSDTQAYRQFGNSVVVPVFAAVAKLLEPKIHQAVTLRQRETADGGRSR
>Mature_476_residues
MQENISVTHARNLIADDAGSEIQAMLSQLLEIYDVKTLVAHLNGLGEQHWSPAIFKRVMMNAAWHRLSDNELSCLKTELP
TPPAHHPHYAFRFIDLFAGIGGIRRGFEAIGGQCVFTSEWNKHAVRTYKANYFCDPLQHRFNEDIRDITLSHREGVSDDE
AAEHIRQHIPQHDVLLAGFPCQPFSLAGVSKKNALGRAHGFACETQGTLFFDVVRIIDARRPALFVLENVKNLKSHDQGN
TFRIIMQTLDELGYDVADAADNGPDDPKIIDGQHFLPQHRERIVLVGFRRDLNLKTDFTLRNIARCYPPRRPTLAELLEP
VVEAKYILTPVLWKYLYRYAKKHQARGNGFGYGMVYPDNPESVARTLSARYYKDGAEILIDRGWDMAKGEVNFDDAGNQQ
HRPRRLTPRECARLMGFEAPQTYQFRIPVSDTQAYRQFGNSVVVPVFAAVAKLLEPKIHQAVTLRQRETADGGRSR

Specific function: This methylase recognizes the double-stranded sequence CCWGG, causes specific methylation on C-2 on both strands [H]

COG id: COG0270

COG function: function code L; Site-specific DNA methylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the C5-methyltransferase family [H]

Homologues:

Organism=Escherichia coli, GI1788271, Length=477, Percent_Identity=83.2285115303983, Blast_Score=808, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018117
- InterPro:   IPR001525 [H]

Pfam domain/function: PF00145 DNA_methylase [H]

EC number: =2.1.1.37 [H]

Molecular weight: Translated: 54095; Mature: 54095

Theoretical pI: Translated: 8.11; Mature: 8.11

Prosite motif: PS00094 C5_MTASE_1 ; PS00095 C5_MTASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQENISVTHARNLIADDAGSEIQAMLSQLLEIYDVKTLVAHLNGLGEQHWSPAIFKRVMM
CCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHH
NAAWHRLSDNELSCLKTELPTPPAHHPHYAFRFIDLFAGIGGIRRGFEAIGGQCVFTSEW
HHHHHHCCCCCHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCC
NKHAVRTYKANYFCDPLQHRFNEDIRDITLSHREGVSDDEAAEHIRQHIPQHDVLLAGFP
CCHHHHEEECCCCCCHHHHHHCCHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCEEEECCC
CQPFSLAGVSKKNALGRAHGFACETQGTLFFDVVRIIDARRPALFVLENVKNLKSHDQGN
CCCCCCCCCCHHHHHHHHCCCEECCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCC
TFRIIMQTLDELGYDVADAADNGPDDPKIIDGQHFLPQHRERIVLVGFRRDLNLKTDFTL
HHHHHHHHHHHHCCCHHHCCCCCCCCCCEECCCCCCCCCCCEEEEEEECCCCCCCCCHHH
RNIARCYPPRRPTLAELLEPVVEAKYILTPVLWKYLYRYAKKHQARGNGFGYGMVYPDNP
HHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCH
ESVARTLSARYYKDGAEILIDRGWDMAKGEVNFDDAGNQQHRPRRLTPRECARLMGFEAP
HHHHHHHHHHHHCCCCCEEEECCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHCCCCC
QTYQFRIPVSDTQAYRQFGNSVVVPVFAAVAKLLEPKIHQAVTLRQRETADGGRSR
CCEEEEECCCHHHHHHHHCCCCHHHHHHHHHHHHCHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MQENISVTHARNLIADDAGSEIQAMLSQLLEIYDVKTLVAHLNGLGEQHWSPAIFKRVMM
CCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHH
NAAWHRLSDNELSCLKTELPTPPAHHPHYAFRFIDLFAGIGGIRRGFEAIGGQCVFTSEW
HHHHHHCCCCCHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCC
NKHAVRTYKANYFCDPLQHRFNEDIRDITLSHREGVSDDEAAEHIRQHIPQHDVLLAGFP
CCHHHHEEECCCCCCHHHHHHCCHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCEEEECCC
CQPFSLAGVSKKNALGRAHGFACETQGTLFFDVVRIIDARRPALFVLENVKNLKSHDQGN
CCCCCCCCCCHHHHHHHHCCCEECCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCC
TFRIIMQTLDELGYDVADAADNGPDDPKIIDGQHFLPQHRERIVLVGFRRDLNLKTDFTL
HHHHHHHHHHHHCCCHHHCCCCCCCCCCEECCCCCCCCCCCEEEEEEECCCCCCCCCHHH
RNIARCYPPRRPTLAELLEPVVEAKYILTPVLWKYLYRYAKKHQARGNGFGYGMVYPDNP
HHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCH
ESVARTLSARYYKDGAEILIDRGWDMAKGEVNFDDAGNQQHRPRRLTPRECARLMGFEAP
HHHHHHHHHHHHCCCCCEEEECCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHCCCCC
QTYQFRIPVSDTQAYRQFGNSVVVPVFAAVAKLLEPKIHQAVTLRQRETADGGRSR
CCEEEEECCCHHHHHHHHCCCCHHHHHHHHHHHHCHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]