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The map label for this gene is cbiB [H]

Identifier: 29141337

GI number: 29141337

Start: 934928

End: 935887

Strand: Direct

Name: cbiB [H]

Synonym: t0839

Alternate gene names: 29141337

Gene position: 934928-935887 (Clockwise)

Preceding gene: 29141336

Following gene: 29141338

Centisome position: 19.51

GC content: 59.58

Gene sequence:

>960_bases
ATGACGATCCTTGCCTGGTGTATCGCCTGGGTGCTGGATTTTATCATCGGCGACCCTCAACACTGGCCCCATCCGGTACG
CTGGATAGGCCGATTAATTACGTTTGTGCAGCGTATTGTGCGTCGCTATTGTCCCGGCGATAAAGCGCTGCGGATTGGCG
GCGGCGTAATGTGGGTTGTGGTTGTCGGGGTGACCTGGGGCGTGGCATGGGGCGTACTGGCGCTGGCGCAGCGGATTCAC
CCCTGGTTTGGCTGGAGCGTCGAAGTCTGGATGATCTTTACCACGCTGGCCGGACGCTCTCTGGCCCGCGCCGCGCAGGA
GGTTGAACGACCGTTGCGGGAGAACGACCTTGCGGAAAGCCGAATAAAACTCTCCTGGATCGTCGGGCGCGATACGTCGC
AACTTCAGCCAGCGCAGATCTATCGCGCCGTGGTGGAAACGGTTGCAGAAAACACCGTTGACGGCATTATCGCGCCGCTC
TTTTTCCTCTTTCTCGGCGGCGCGCCGCTGGCGATGGCCTACAAAGCCGTCAATACCCTGGATTCAATGGTGGGCTACAA
ACATGAAAAATACCGGGCGATTGGTATGGTCAGCGCCCGTATGGACGACGTAGCGAACTATTTGCCCGCCCGACTGAGCT
GGCTGTTGCTGGGCATTGCGGCAGGACTGTGTCGCCTGAGTGGTTGGCGGGCGCTGCGTATTGGCTGGCGTGACCGTTAT
AACCACAGTAGCCCAAATTGCGCCTGGTCGGAAGCGTGTGTGGCTGGCGCCTTAGGAATCCAGCTCGGTGGCCCAAATAA
CTACTTTGGCGAGCGTGTGGACAAGCCCTGGATCGGCGACGCGCAGCGCGGTATTTCCGTGGACGATATTTCCCGAACGA
TTCGATTGATGTGGGTCGCTTCAACCCTGGCGCTGGCGCTGTTTATTGCGGCGCGGTGCGGGTTATCTGGCGTGGCCTGA

Upstream 100 bases:

>100_bases
GCTGGCAGACAGGCAACACGTTTGCCAGCTATCTGCACGTCCATTTCGCCCAGCGTCCGGAAATGTTGCAGCACTGGCTG
GCGGCGGCGAGGCGAGTATT

Downstream 100 bases:

>100_bases
GGACGACAGTATGCATTATATCCAGCAGCCCCAAACCATTGAGGCCAACAGTTTCACCATTATTAGCGACATTATTCGCG
AAACGCGTCCTGATTACCGT

Product: cobalamin biosynthesis protein

Products: ADP; phosphate; Adenosyl cobinamide phosphate; Adenosyl cobinamide

Alternate protein names: NA

Number of amino acids: Translated: 319; Mature: 318

Protein sequence:

>319_residues
MTILAWCIAWVLDFIIGDPQHWPHPVRWIGRLITFVQRIVRRYCPGDKALRIGGGVMWVVVVGVTWGVAWGVLALAQRIH
PWFGWSVEVWMIFTTLAGRSLARAAQEVERPLRENDLAESRIKLSWIVGRDTSQLQPAQIYRAVVETVAENTVDGIIAPL
FFLFLGGAPLAMAYKAVNTLDSMVGYKHEKYRAIGMVSARMDDVANYLPARLSWLLLGIAAGLCRLSGWRALRIGWRDRY
NHSSPNCAWSEACVAGALGIQLGGPNNYFGERVDKPWIGDAQRGISVDDISRTIRLMWVASTLALALFIAARCGLSGVA

Sequences:

>Translated_319_residues
MTILAWCIAWVLDFIIGDPQHWPHPVRWIGRLITFVQRIVRRYCPGDKALRIGGGVMWVVVVGVTWGVAWGVLALAQRIH
PWFGWSVEVWMIFTTLAGRSLARAAQEVERPLRENDLAESRIKLSWIVGRDTSQLQPAQIYRAVVETVAENTVDGIIAPL
FFLFLGGAPLAMAYKAVNTLDSMVGYKHEKYRAIGMVSARMDDVANYLPARLSWLLLGIAAGLCRLSGWRALRIGWRDRY
NHSSPNCAWSEACVAGALGIQLGGPNNYFGERVDKPWIGDAQRGISVDDISRTIRLMWVASTLALALFIAARCGLSGVA
>Mature_318_residues
TILAWCIAWVLDFIIGDPQHWPHPVRWIGRLITFVQRIVRRYCPGDKALRIGGGVMWVVVVGVTWGVAWGVLALAQRIHP
WFGWSVEVWMIFTTLAGRSLARAAQEVERPLRENDLAESRIKLSWIVGRDTSQLQPAQIYRAVVETVAENTVDGIIAPLF
FLFLGGAPLAMAYKAVNTLDSMVGYKHEKYRAIGMVSARMDDVANYLPARLSWLLLGIAAGLCRLSGWRALRIGWRDRYN
HSSPNCAWSEACVAGALGIQLGGPNNYFGERVDKPWIGDAQRGISVDDISRTIRLMWVASTLALALFIAARCGLSGVA

Specific function: Converts cobyric acid to cobinamide by the addition of aminopropanol on the F carboxylic group. However, the true cosubstrate could be (R)-1-amino-2-propanol O-2-phosphate, leading to cobinamide phosphate [H]

COG id: COG1270

COG function: function code H; Cobalamin biosynthesis protein CobD/CbiB

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the CobD/CbiB family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004485 [H]

Pfam domain/function: PF03186 CobD_Cbib [H]

EC number: 6.3.1.10

Molecular weight: Translated: 35444; Mature: 35313

Theoretical pI: Translated: 9.64; Mature: 9.64

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTILAWCIAWVLDFIIGDPQHWPHPVRWIGRLITFVQRIVRRYCPGDKALRIGGGVMWVV
CHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCHHHHH
VVGVTWGVAWGVLALAQRIHPWFGWSVEVWMIFTTLAGRSLARAAQEVERPLRENDLAES
HHHHHHHHHHHHHHHHHHHCHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHH
RIKLSWIVGRDTSQLQPAQIYRAVVETVAENTVDGIIAPLFFLFLGGAPLAMAYKAVNTL
HEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHH
DSMVGYKHEKYRAIGMVSARMDDVANYLPARLSWLLLGIAAGLCRLSGWRALRIGWRDRY
HHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECHHHCC
NHSSPNCAWSEACVAGALGIQLGGPNNYFGERVDKPWIGDAQRGISVDDISRTIRLMWVA
CCCCCCCCHHHHHHHHHHHEEECCCCCHHHHHCCCCCCCCHHCCCCHHHHHHHHHHHHHH
STLALALFIAARCGLSGVA
HHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
TILAWCIAWVLDFIIGDPQHWPHPVRWIGRLITFVQRIVRRYCPGDKALRIGGGVMWVV
HHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCHHHHH
VVGVTWGVAWGVLALAQRIHPWFGWSVEVWMIFTTLAGRSLARAAQEVERPLRENDLAES
HHHHHHHHHHHHHHHHHHHCHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHH
RIKLSWIVGRDTSQLQPAQIYRAVVETVAENTVDGIIAPLFFLFLGGAPLAMAYKAVNTL
HEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHH
DSMVGYKHEKYRAIGMVSARMDDVANYLPARLSWLLLGIAAGLCRLSGWRALRIGWRDRY
HHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECHHHCC
NHSSPNCAWSEACVAGALGIQLGGPNNYFGERVDKPWIGDAQRGISVDDISRTIRLMWVA
CCCCCCCCHHHHHHHHHHHEEECCCCCHHHHHCCCCCCCCHHCCCCHHHHHHHHHHHHHH
STLALALFIAARCGLSGVA
HHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; adenosylcobyric acid; (R)-1-aminopropan-2-yl phosphate; (R)-1-aminopropan-2-ol

Specific reaction: (1) ATP + adenosylcobyric acid + (R)-1-aminopropan-2-yl phosphate = ADP + phosphate + adenosylcobinamide phosphate; (2) ATP + adenosylcobyric acid + (R)-1-aminopropan-2-ol = ADP + phosphate + adenosylcobinamide

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA