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The map label for this gene is pduF [H]

Identifier: 29141334

GI number: 29141334

Start: 931030

End: 931824

Strand: Direct

Name: pduF [H]

Synonym: t0837

Alternate gene names: 29141334

Gene position: 931030-931824 (Clockwise)

Preceding gene: 29141313

Following gene: 29141335

Centisome position: 19.43

GC content: 50.44

Gene sequence:

>795_bases
ATGAATGATTCTCTAAAGGCGCAATGTGGTGCGGAATTTTTAGGCACCGGACTCTTTTTGTTTTTTGGTATCGGTTGTTT
GAGCGCGCTCAAAGTCGCAGGCGCCAGCCTCGGATTATGGGAGATTTGCATTATTTGGGGGCTTGGAATTTCACTGGCCG
TTTACCTTACTGCCGGAATCTCCGGCGGACATCTTAACCCTGCGGTGACGATTGCGCTGTGGCTGTTTGCCTGCTTCCCC
AAACAGAAAGTATTGCCTTATATTATTGCCCAGTTTGCCGGCGCTTTTGGCGGCGATCTGCTGGCGTATGTGCTTTATAG
TAGCCTTTTTACTGAATTTGAGACTGCGCATCATATGGTGCGCGGTAGCGTCGAAAGTTTGCAATTAGCCAGCATTTTCA
GTACCTATCCGGCCGCAGCGCTAAATGTATGGCAGGCTGCGTTGGTGGAAGTGGTCATTACGTCGATCCTGATGGGCATG
ATCATGGCATTGACGGATGATGGCAATGGTATTCCTAAAGGGCCGCTTGCGCCTTTGCTTATTGGGATACTGGTTGCCGT
TATCGGCGCGTCTACTGGACCCCTTACCGGTTTTGCAATGAACCCGGCGCGTGATTTTGGTCCCAAACTGTTCACATGGT
TAGCGGGGTGGGGGAACATGGCGATGAGCGGTGGGCGAGAGATCCCTTATTTTATCGTGCCGATTGTCGCGCCTGTTATC
GGCGCCTGTGCTGGAGCAGCCATATATCGCTATTTTATTGGTAAGAATTTACCTTGTAACCGCTGTAAACTGTAA

Upstream 100 bases:

>100_bases
CAATTTACAGGCGGCACTTTTTATAGTGAGGTTAGCTGTACTCTACCGCCAGCCGAAACTTGCGCTGGCCAGGACACGAA
CTTTGCTCAGAAGGTGTCAC

Downstream 100 bases:

>100_bases
GAAAATGCGAACCAGATTCACAGCAATGATGTATCGTAAAAACAGGTGAGATAATTTCGTGATCTGCTTTCACGAACTGA
TCAAAACGTCTCCCAGCAAT

Product: propanediol diffusion facilitator

Products: glycerol [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 264; Mature: 264

Protein sequence:

>264_residues
MNDSLKAQCGAEFLGTGLFLFFGIGCLSALKVAGASLGLWEICIIWGLGISLAVYLTAGISGGHLNPAVTIALWLFACFP
KQKVLPYIIAQFAGAFGGDLLAYVLYSSLFTEFETAHHMVRGSVESLQLASIFSTYPAAALNVWQAALVEVVITSILMGM
IMALTDDGNGIPKGPLAPLLIGILVAVIGASTGPLTGFAMNPARDFGPKLFTWLAGWGNMAMSGGREIPYFIVPIVAPVI
GACAGAAIYRYFIGKNLPCNRCKL

Sequences:

>Translated_264_residues
MNDSLKAQCGAEFLGTGLFLFFGIGCLSALKVAGASLGLWEICIIWGLGISLAVYLTAGISGGHLNPAVTIALWLFACFP
KQKVLPYIIAQFAGAFGGDLLAYVLYSSLFTEFETAHHMVRGSVESLQLASIFSTYPAAALNVWQAALVEVVITSILMGM
IMALTDDGNGIPKGPLAPLLIGILVAVIGASTGPLTGFAMNPARDFGPKLFTWLAGWGNMAMSGGREIPYFIVPIVAPVI
GACAGAAIYRYFIGKNLPCNRCKL
>Mature_264_residues
MNDSLKAQCGAEFLGTGLFLFFGIGCLSALKVAGASLGLWEICIIWGLGISLAVYLTAGISGGHLNPAVTIALWLFACFP
KQKVLPYIIAQFAGAFGGDLLAYVLYSSLFTEFETAHHMVRGSVESLQLASIFSTYPAAALNVWQAALVEVVITSILMGM
IMALTDDGNGIPKGPLAPLLIGILVAVIGASTGPLTGFAMNPARDFGPKLFTWLAGWGNMAMSGGREIPYFIVPIVAPVI
GACAGAAIYRYFIGKNLPCNRCKL

Specific function: May facilitate the diffusion of propanediol [H]

COG id: COG0580

COG function: function code G; Glycerol uptake facilitator and related permeases (Major Intrinsic Protein Family)

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MIP/aquaporin (TC 1.A.8) family [H]

Homologues:

Organism=Homo sapiens, GI157266307, Length=260, Percent_Identity=37.3076923076923, Blast_Score=145, Evalue=4e-35,
Organism=Homo sapiens, GI4502187, Length=257, Percent_Identity=36.1867704280156, Blast_Score=145, Evalue=4e-35,
Organism=Homo sapiens, GI22538420, Length=256, Percent_Identity=35.546875, Blast_Score=143, Evalue=2e-34,
Organism=Homo sapiens, GI4826645, Length=256, Percent_Identity=36.328125, Blast_Score=137, Evalue=7e-33,
Organism=Homo sapiens, GI310133356, Length=218, Percent_Identity=31.651376146789, Blast_Score=96, Evalue=4e-20,
Organism=Homo sapiens, GI310114181, Length=218, Percent_Identity=31.651376146789, Blast_Score=96, Evalue=4e-20,
Organism=Homo sapiens, GI45446752, Length=202, Percent_Identity=34.6534653465347, Blast_Score=80, Evalue=2e-15,
Organism=Homo sapiens, GI4502183, Length=246, Percent_Identity=32.9268292682927, Blast_Score=73, Evalue=2e-13,
Organism=Homo sapiens, GI4502179, Length=244, Percent_Identity=31.9672131147541, Blast_Score=72, Evalue=7e-13,
Organism=Homo sapiens, GI6912506, Length=254, Percent_Identity=29.9212598425197, Blast_Score=69, Evalue=4e-12,
Organism=Homo sapiens, GI86792455, Length=202, Percent_Identity=33.1683168316832, Blast_Score=68, Evalue=8e-12,
Organism=Escherichia coli, GI1790362, Length=262, Percent_Identity=67.9389312977099, Blast_Score=379, Evalue=1e-106,
Organism=Escherichia coli, GI1787101, Length=220, Percent_Identity=30.9090909090909, Blast_Score=70, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI71992966, Length=245, Percent_Identity=37.5510204081633, Blast_Score=143, Evalue=9e-35,
Organism=Caenorhabditis elegans, GI71994009, Length=252, Percent_Identity=36.1111111111111, Blast_Score=141, Evalue=4e-34,
Organism=Caenorhabditis elegans, GI17533613, Length=252, Percent_Identity=34.9206349206349, Blast_Score=127, Evalue=5e-30,
Organism=Caenorhabditis elegans, GI32564052, Length=252, Percent_Identity=34.9206349206349, Blast_Score=127, Evalue=6e-30,
Organism=Caenorhabditis elegans, GI17544068, Length=246, Percent_Identity=38.6178861788618, Blast_Score=127, Evalue=9e-30,
Organism=Caenorhabditis elegans, GI17531431, Length=251, Percent_Identity=31.4741035856574, Blast_Score=112, Evalue=2e-25,
Organism=Caenorhabditis elegans, GI17531429, Length=251, Percent_Identity=31.4741035856574, Blast_Score=112, Evalue=2e-25,
Organism=Caenorhabditis elegans, GI71992961, Length=245, Percent_Identity=34.6938775510204, Blast_Score=109, Evalue=2e-24,
Organism=Caenorhabditis elegans, GI71993722, Length=253, Percent_Identity=27.2727272727273, Blast_Score=74, Evalue=6e-14,
Organism=Caenorhabditis elegans, GI17558372, Length=260, Percent_Identity=26.9230769230769, Blast_Score=74, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI212646268, Length=236, Percent_Identity=30.0847457627119, Blast_Score=70, Evalue=8e-13,
Organism=Saccharomyces cerevisiae, GI6321054, Length=212, Percent_Identity=41.5094339622642, Blast_Score=142, Evalue=4e-35,
Organism=Saccharomyces cerevisiae, GI6322985, Length=233, Percent_Identity=30.4721030042918, Blast_Score=87, Evalue=3e-18,
Organism=Drosophila melanogaster, GI45550503, Length=248, Percent_Identity=26.2096774193548, Blast_Score=79, Evalue=3e-15,
Organism=Drosophila melanogaster, GI24762344, Length=249, Percent_Identity=28.9156626506024, Blast_Score=73, Evalue=2e-13,
Organism=Drosophila melanogaster, GI24762346, Length=249, Percent_Identity=28.9156626506024, Blast_Score=73, Evalue=2e-13,
Organism=Drosophila melanogaster, GI24762348, Length=249, Percent_Identity=28.9156626506024, Blast_Score=73, Evalue=2e-13,
Organism=Drosophila melanogaster, GI20130305, Length=249, Percent_Identity=28.9156626506024, Blast_Score=73, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012269
- InterPro:   IPR000425
- InterPro:   IPR022357 [H]

Pfam domain/function: PF00230 MIP [H]

EC number: NA

Molecular weight: Translated: 27787; Mature: 27787

Theoretical pI: Translated: 7.73; Mature: 7.73

Prosite motif: PS00013 PROKAR_LIPOPROTEIN ; PS00221 MIP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.7 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
5.7 %Cys+Met (Translated Protein)
2.7 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
5.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHHHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure
MNDSLKAQCGAEFLGTGLFLFFGIGCLSALKVAGASLGLWEICIIWGLGISLAVYLTAGI
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCC
SGGHLNPAVTIALWLFACFPKQKVLPYIIAQFAGAFGGDLLAYVLYSSLFTEFETAHHMV
CCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RGSVESLQLASIFSTYPAAALNVWQAALVEVVITSILMGMIMALTDDGNGIPKGPLAPLL
HCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHH
IGILVAVIGASTGPLTGFAMNPARDFGPKLFTWLAGWGNMAMSGGREIPYFIVPIVAPVI
HHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHH
GACAGAAIYRYFIGKNLPCNRCKL
HHHHHHHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: glycerol [Periplasm] [C]

Specific reaction: glycerol [Periplasm] = glycerol [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 8071226; 9352910; 11677609 [H]