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The map label for this gene is wcaJ [H]

Identifier: 29141270

GI number: 29141270

Start: 859006

End: 860400

Strand: Direct

Name: wcaJ [H]

Synonym: t0769

Alternate gene names: 29141270

Gene position: 859006-860400 (Clockwise)

Preceding gene: 29141269

Following gene: 29141271

Centisome position: 17.93

GC content: 53.55

Gene sequence:

>1395_bases
ATGACAAATCTAAAAAAGCGCGAACGAGCCAAAACCAATGCATCGTTAATCTCTATGGTGCAACGCTTTTCAGATATCAC
CATCATGTTTGGCGGGCTGTGGTTCATCTGTGAAGTCAGTGGACTGCCATTCCTGTACATGCACCTGTTGGTGGCGCTGA
TTACGCTGGTTGTTTTTCAAATGCTGGGCGGGATGACCGATTTTTATCGCTCCTGGCGCGGCGTCAAAATGTCGACAGAG
TTAACGCTGTTACTGCAAAACTGGACGCTGAGCCTGATCTTCAGCGCCGGTCTGGTGGCGTTTAATGATGACTTTGACAA
CCGCCTCGCCACCTGGTTGGCCTGGTATGCGCTGTCCAGCCTGGGCTTAGTGTTATGCCGTTCATTTATTCGTTTCGGCG
CGGGCTGGCTACGCAATCGTGGTTATAACACTCGCCGGGGCGCCGTCGCCGGGGATTTGCCTGCGGGCCAGGCGTTGCTG
GATAGCTTCCGTAATGAGCCGTGGCTGGGTTTTGAGGTGGTGGGCGTCTATCACGATCCTAAACCGGGCGGCGTCTCTGC
TGACTGGGCCGGGAACTTCGAACAGTTAATTGACGATGCGAAAGCCTCGCGAATTCACAATGTTTATATCGCCATGTCAA
TGAGCGACGGCGCCATGGTGAAAAAACTGGTGCGCGAGCTGGCCGACACCACCTGCTCGGTGATCCTTATTCCCGATGTG
TTCACCTTTAATATTCTCCACTCGCGCGTGGAGGAGATGAGCGGCGTTCCGGTCGTTCCGCTGTATGACACGCCGCTATC
AGGGATTAACCGTCTGCTTAAACGGGCAGAAGATATCGTGCTGGCGTCGCTGATTCTGCTGCTCATCTCACCGGTACTGT
GCTGCATTGCGCCGGCGGTCAAATTGAGCTCGCCCGGGCCGGTGATTTTCCGTCAGACGCGCTACGGGATGGACGGCAAG
CCGAGCAAAGTCTGGAGGTTCCGCTCAATGAAGGTGATGGAGAACGATAAGGTCGTTACCCAGGCGACGCAAAACGATCC
GCGCGTCACCCGGGTAGGTAACTTTTTGCGTCGTACCTCGCTGGATGAATTACCGCAGTTTATCAACGTATTAACCGGCG
GGATGTCTATCGTCGGTCCTCGCCCGCACGCGGTGGCGCATAACGAACAGTATCGTCAGTTGATTGAAGGTTACATGCTA
CGCCACAAGATGAAGCCGGGCATCACCGGCTGGGCGCAAATTAATGGCTGGCGCGGTGAGACCGATACGCTGGAGAAAAT
GGAAAAACGCGTGGAATTCGACCTGGAATACATCCGCGAATGGAGTATTTGGTTCGATATCAAAATCGTTTTTCTGACGA
TTTTTAAAGGGTTTGTTAATAAAGCGGCGTATTAA

Upstream 100 bases:

>100_bases
TGATGGAAGCGCGAACACGCACTCTGCTGGCGCTGCTGAATCAGTAACGTCAGATCTCCCCTTACCGCCTGCGGGTAAGG
GGCCAATCACAGGAACAACG

Downstream 100 bases:

>100_bases
GAACCTGACCCACAAGGCTATTTTACTTGCCATATTGCACCAGGGCAGTGCTCAATCTGGCTGCGCCAATGACGCCTTGT
GAACAGGTTCTGGAGAAAAG

Product: UDP-glucose lipid carrier transferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 464; Mature: 463

Protein sequence:

>464_residues
MTNLKKRERAKTNASLISMVQRFSDITIMFGGLWFICEVSGLPFLYMHLLVALITLVVFQMLGGMTDFYRSWRGVKMSTE
LTLLLQNWTLSLIFSAGLVAFNDDFDNRLATWLAWYALSSLGLVLCRSFIRFGAGWLRNRGYNTRRGAVAGDLPAGQALL
DSFRNEPWLGFEVVGVYHDPKPGGVSADWAGNFEQLIDDAKASRIHNVYIAMSMSDGAMVKKLVRELADTTCSVILIPDV
FTFNILHSRVEEMSGVPVVPLYDTPLSGINRLLKRAEDIVLASLILLLISPVLCCIAPAVKLSSPGPVIFRQTRYGMDGK
PSKVWRFRSMKVMENDKVVTQATQNDPRVTRVGNFLRRTSLDELPQFINVLTGGMSIVGPRPHAVAHNEQYRQLIEGYML
RHKMKPGITGWAQINGWRGETDTLEKMEKRVEFDLEYIREWSIWFDIKIVFLTIFKGFVNKAAY

Sequences:

>Translated_464_residues
MTNLKKRERAKTNASLISMVQRFSDITIMFGGLWFICEVSGLPFLYMHLLVALITLVVFQMLGGMTDFYRSWRGVKMSTE
LTLLLQNWTLSLIFSAGLVAFNDDFDNRLATWLAWYALSSLGLVLCRSFIRFGAGWLRNRGYNTRRGAVAGDLPAGQALL
DSFRNEPWLGFEVVGVYHDPKPGGVSADWAGNFEQLIDDAKASRIHNVYIAMSMSDGAMVKKLVRELADTTCSVILIPDV
FTFNILHSRVEEMSGVPVVPLYDTPLSGINRLLKRAEDIVLASLILLLISPVLCCIAPAVKLSSPGPVIFRQTRYGMDGK
PSKVWRFRSMKVMENDKVVTQATQNDPRVTRVGNFLRRTSLDELPQFINVLTGGMSIVGPRPHAVAHNEQYRQLIEGYML
RHKMKPGITGWAQINGWRGETDTLEKMEKRVEFDLEYIREWSIWFDIKIVFLTIFKGFVNKAAY
>Mature_463_residues
TNLKKRERAKTNASLISMVQRFSDITIMFGGLWFICEVSGLPFLYMHLLVALITLVVFQMLGGMTDFYRSWRGVKMSTEL
TLLLQNWTLSLIFSAGLVAFNDDFDNRLATWLAWYALSSLGLVLCRSFIRFGAGWLRNRGYNTRRGAVAGDLPAGQALLD
SFRNEPWLGFEVVGVYHDPKPGGVSADWAGNFEQLIDDAKASRIHNVYIAMSMSDGAMVKKLVRELADTTCSVILIPDVF
TFNILHSRVEEMSGVPVVPLYDTPLSGINRLLKRAEDIVLASLILLLISPVLCCIAPAVKLSSPGPVIFRQTRYGMDGKP
SKVWRFRSMKVMENDKVVTQATQNDPRVTRVGNFLRRTSLDELPQFINVLTGGMSIVGPRPHAVAHNEQYRQLIEGYMLR
HKMKPGITGWAQINGWRGETDTLEKMEKRVEFDLEYIREWSIWFDIKIVFLTIFKGFVNKAAY

Specific function: Slime polysaccharide colanic acid biosynthesis. [C]

COG id: COG2148

COG function: function code M; Sugar transferases involved in lipopolysaccharide synthesis

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial sugar transferase family [H]

Homologues:

Organism=Escherichia coli, GI1788360, Length=464, Percent_Identity=88.3620689655172, Blast_Score=866, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003362
- InterPro:   IPR017475
- InterPro:   IPR017473 [H]

Pfam domain/function: PF02397 Bac_transf [H]

EC number: NA

Molecular weight: Translated: 52511; Mature: 52380

Theoretical pI: Translated: 9.63; Mature: 9.63

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTNLKKRERAKTNASLISMVQRFSDITIMFGGLWFICEVSGLPFLYMHLLVALITLVVFQ
CCCCCHHHHHHHHHHHHHHHHHHHCHHHHHCCEEEEHHHCCCHHHHHHHHHHHHHHHHHH
MLGGMTDFYRSWRGVKMSTELTLLLQNWTLSLIFSAGLVAFNDDFDNRLATWLAWYALSS
HHCCHHHHHHHHCCCEECHHEEEHHHHHHHHHHHHCCEEEECCCHHHHHHHHHHHHHHHH
LGLVLCRSFIRFGAGWLRNRGYNTRRGAVAGDLPAGQALLDSFRNEPWLGFEVVGVYHDP
HHHHHHHHHHHHCCHHHHCCCCCCCCCCCCCCCCCCHHHHHHHCCCCCCCEEEEEEEECC
KPGGVSADWAGNFEQLIDDAKASRIHNVYIAMSMSDGAMVKKLVRELADTTCSVILIPDV
CCCCCCCCCCCCHHHHHHHHHHHHEEEEEEEEECCCCHHHHHHHHHHHHCCEEEEEECCH
FTFNILHSRVEEMSGVPVVPLYDTPLSGINRLLKRAEDIVLASLILLLISPVLCCIAPAV
HHHHHHHHHHHHHCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KLSSPGPVIFRQTRYGMDGKPSKVWRFRSMKVMENDKVVTQATQNDPRVTRVGNFLRRTS
CCCCCCCEEEEECCCCCCCCCHHHHHHHHHHHHCCCCEEEECCCCCCCHHHHHHHHHHCC
LDELPQFINVLTGGMSIVGPRPHAVAHNEQYRQLIEGYMLRHKMKPGITGWAQINGWRGE
HHHHHHHHHHHHCCCEEECCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCEEEECCCCCC
TDTLEKMEKRVEFDLEYIREWSIWFDIKIVFLTIFKGFVNKAAY
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
TNLKKRERAKTNASLISMVQRFSDITIMFGGLWFICEVSGLPFLYMHLLVALITLVVFQ
CCCCHHHHHHHHHHHHHHHHHHHCHHHHHCCEEEEHHHCCCHHHHHHHHHHHHHHHHHH
MLGGMTDFYRSWRGVKMSTELTLLLQNWTLSLIFSAGLVAFNDDFDNRLATWLAWYALSS
HHCCHHHHHHHHCCCEECHHEEEHHHHHHHHHHHHCCEEEECCCHHHHHHHHHHHHHHHH
LGLVLCRSFIRFGAGWLRNRGYNTRRGAVAGDLPAGQALLDSFRNEPWLGFEVVGVYHDP
HHHHHHHHHHHHCCHHHHCCCCCCCCCCCCCCCCCCHHHHHHHCCCCCCCEEEEEEEECC
KPGGVSADWAGNFEQLIDDAKASRIHNVYIAMSMSDGAMVKKLVRELADTTCSVILIPDV
CCCCCCCCCCCCHHHHHHHHHHHHEEEEEEEEECCCCHHHHHHHHHHHHCCEEEEEECCH
FTFNILHSRVEEMSGVPVVPLYDTPLSGINRLLKRAEDIVLASLILLLISPVLCCIAPAV
HHHHHHHHHHHHHCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KLSSPGPVIFRQTRYGMDGKPSKVWRFRSMKVMENDKVVTQATQNDPRVTRVGNFLRRTS
CCCCCCCEEEEECCCCCCCCCHHHHHHHHHHHHCCCCEEEECCCCCCCHHHHHHHHHHCC
LDELPQFINVLTGGMSIVGPRPHAVAHNEQYRQLIEGYMLRHKMKPGITGWAQINGWRGE
HHHHHHHHHHHHCCCEEECCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCEEEECCCCCC
TDTLEKMEKRVEFDLEYIREWSIWFDIKIVFLTIFKGFVNKAAY
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8759852; 9097040; 9278503 [H]