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The map label for this gene is manB [H]

Identifier: 29141269

GI number: 29141269

Start: 857582

End: 858952

Strand: Direct

Name: manB [H]

Synonym: t0768

Alternate gene names: 29141269

Gene position: 857582-858952 (Clockwise)

Preceding gene: 29141268

Following gene: 29141270

Centisome position: 17.9

GC content: 61.71

Gene sequence:

>1371_bases
ATGACAAAATTAACCTGTTTCAAGGCCTACGACATTCGCGGCAGGCTGGGCGAAGAGCTCAATGAAGATATCGCGTGGCG
GATTGGCCGCGCCTATGGCGAATATTTAAAACCGAAAACCATCGTGCTGGGCGGCGACGTGCGCCTGACCAGCGAGGTGC
TGAAGATGGCGCTGGCAAAAGGGTTACAGGACGCGGGCGTCGACGTGCTGGATATCGGTATGTCCGGCACCGAAGAGATT
TACTTCGCCACCTTCCACCTCGGCGTGGACGGCGGTATTGAAGTGACCGCCAGCCATAACCCGATGGACTACAACGGTAT
GAAGCTGGTGCGCGAGGGCGCCCGCCCGATAAGCGGCGACACCGGTCTGCGCGACGTTCAGCGCCTGGCGGAAGCGGGCG
ACTTCCCGCCGGTCAACGACGCCACGCGCGGCAGCTACAGACAAATCAGCATGCGCGACGCCTACATCGACCACCTGCTC
GGCTACATCAGTGTTAACAATCTCACCCCGCTGAAGCTGGTGTTTAACGCCGGCAACGGCGCGGCGGGGCCGGTCATTGA
CGCCATTGAAGCGCGCCTGAAGGCGCTGGGCGCGCCGGTGGAGTTCATCAAAATCCACAACACCCCGGACGGCACCTTCC
CGAAAGGCATTCCCAACCCGTTGCTGCCGGAGTGTCGCGACGACACCCGCAAGGCCGTTATTGAACACGGGGCGGACATG
GGCATCGCCTTTGACGGCGACTTCGACCGCTGCTTCCTGTTCGATGAGAAAGGGAAGTTTATCGAGGGGTACTACATCGT
CGGGCTGCTGGCGGAAGCGTTTCTGGAAAAACACCCGGGGGCGAAGATTATCCACGACCCGCGCCTGACCTGGAACACCG
AGGCAGTGGTGACGGCGGCGGGCGGGACGCCGATGATGTCAAAAACCGGGCACGCCTTTATCAAGGAGCGGATGCGTACG
GAGGATGCCATTTATGGCGGGGAAATGAGCGCGCATCACTATTTCCGTGATTTTGCCTACTGCGACAGCGGGATGATCCC
GTGGCTGCTGGTGGCGGAACTGGTATGCCTGAAGGGGCAGTCGCTGGGTGAACTGGTTCGCGACCGGATGGCGGCGTTCC
CGGCGAGCGGGGAAATCAACAGCAGACTGGCGGAGCCGGCGGCGGCCATCGCGCGGGTGGAAGCGCACTTCGCGGAAGAG
GCGCAGGCAGTGGACCGCACGGACGGCCTCAGCATGTCGTTCGCCGACTGGCGTTTCAACCTGCGCTCGTCGAACACCGA
ACCGGTGGTGCGCCTGAATGTGGAATCACGCGGCGATATTCCGCTGATGGAAGCGCGAACACGCACTCTGCTGGCGCTGC
TGAATCAGTAA

Upstream 100 bases:

>100_bases
TATGCCCGGTAAGCGCAACGCGCCATCCGGCAATGAATAACAATAACGATGTTGGCCATTTGCGGTCAGGGCTAACTGTT
GCCTGAAGAAGGGTAAGCAC

Downstream 100 bases:

>100_bases
CGTCAGATCTCCCCTTACCGCCTGCGGGTAAGGGGCCAATCACAGGAACAACGATGACAAATCTAAAAAAGCGCGAACGA
GCCAAAACCAATGCATCGTT

Product: phosphomannomutase

Products: NA

Alternate protein names: PMM [H]

Number of amino acids: Translated: 456; Mature: 455

Protein sequence:

>456_residues
MTKLTCFKAYDIRGRLGEELNEDIAWRIGRAYGEYLKPKTIVLGGDVRLTSEVLKMALAKGLQDAGVDVLDIGMSGTEEI
YFATFHLGVDGGIEVTASHNPMDYNGMKLVREGARPISGDTGLRDVQRLAEAGDFPPVNDATRGSYRQISMRDAYIDHLL
GYISVNNLTPLKLVFNAGNGAAGPVIDAIEARLKALGAPVEFIKIHNTPDGTFPKGIPNPLLPECRDDTRKAVIEHGADM
GIAFDGDFDRCFLFDEKGKFIEGYYIVGLLAEAFLEKHPGAKIIHDPRLTWNTEAVVTAAGGTPMMSKTGHAFIKERMRT
EDAIYGGEMSAHHYFRDFAYCDSGMIPWLLVAELVCLKGQSLGELVRDRMAAFPASGEINSRLAEPAAAIARVEAHFAEE
AQAVDRTDGLSMSFADWRFNLRSSNTEPVVRLNVESRGDIPLMEARTRTLLALLNQ

Sequences:

>Translated_456_residues
MTKLTCFKAYDIRGRLGEELNEDIAWRIGRAYGEYLKPKTIVLGGDVRLTSEVLKMALAKGLQDAGVDVLDIGMSGTEEI
YFATFHLGVDGGIEVTASHNPMDYNGMKLVREGARPISGDTGLRDVQRLAEAGDFPPVNDATRGSYRQISMRDAYIDHLL
GYISVNNLTPLKLVFNAGNGAAGPVIDAIEARLKALGAPVEFIKIHNTPDGTFPKGIPNPLLPECRDDTRKAVIEHGADM
GIAFDGDFDRCFLFDEKGKFIEGYYIVGLLAEAFLEKHPGAKIIHDPRLTWNTEAVVTAAGGTPMMSKTGHAFIKERMRT
EDAIYGGEMSAHHYFRDFAYCDSGMIPWLLVAELVCLKGQSLGELVRDRMAAFPASGEINSRLAEPAAAIARVEAHFAEE
AQAVDRTDGLSMSFADWRFNLRSSNTEPVVRLNVESRGDIPLMEARTRTLLALLNQ
>Mature_455_residues
TKLTCFKAYDIRGRLGEELNEDIAWRIGRAYGEYLKPKTIVLGGDVRLTSEVLKMALAKGLQDAGVDVLDIGMSGTEEIY
FATFHLGVDGGIEVTASHNPMDYNGMKLVREGARPISGDTGLRDVQRLAEAGDFPPVNDATRGSYRQISMRDAYIDHLLG
YISVNNLTPLKLVFNAGNGAAGPVIDAIEARLKALGAPVEFIKIHNTPDGTFPKGIPNPLLPECRDDTRKAVIEHGADMG
IAFDGDFDRCFLFDEKGKFIEGYYIVGLLAEAFLEKHPGAKIIHDPRLTWNTEAVVTAAGGTPMMSKTGHAFIKERMRTE
DAIYGGEMSAHHYFRDFAYCDSGMIPWLLVAELVCLKGQSLGELVRDRMAAFPASGEINSRLAEPAAAIARVEAHFAEEA
QAVDRTDGLSMSFADWRFNLRSSNTEPVVRLNVESRGDIPLMEARTRTLLALLNQ

Specific function: Involved in GDP-mannose biosynthesis which serves as the activated sugar nucleotide precursor for mannose residues in cell surface polysaccharides. This enzyme participates in synthesis of the LPS O antigen [H]

COG id: COG1109

COG function: function code G; Phosphomannomutase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phosphohexose mutase family [H]

Homologues:

Organism=Escherichia coli, GI1788361, Length=456, Percent_Identity=88.3771929824561, Blast_Score=852, Evalue=0.0,
Organism=Escherichia coli, GI1789566, Length=463, Percent_Identity=26.133909287257, Blast_Score=79, Evalue=8e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005844
- InterPro:   IPR016055
- InterPro:   IPR005845
- InterPro:   IPR005846
- InterPro:   IPR005843
- InterPro:   IPR016066
- InterPro:   IPR005841 [H]

Pfam domain/function: PF02878 PGM_PMM_I; PF02879 PGM_PMM_II; PF02880 PGM_PMM_III; PF00408 PGM_PMM_IV [H]

EC number: =5.4.2.8 [H]

Molecular weight: Translated: 50044; Mature: 49913

Theoretical pI: Translated: 5.24; Mature: 5.24

Prosite motif: PS00710 PGM_PMM

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKLTCFKAYDIRGRLGEELNEDIAWRIGRAYGEYLKPKTIVLGGDVRLTSEVLKMALAK
CCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHHHHC
GLQDAGVDVLDIGMSGTEEIYFATFHLGVDGGIEVTASHNPMDYNGMKLVREGARPISGD
CHHHCCCCEEEECCCCCCEEEEEEEEEECCCCEEEEECCCCCCCCHHHHHHHCCCCCCCC
TGLRDVQRLAEAGDFPPVNDATRGSYRQISMRDAYIDHLLGYISVNNLTPLKLVFNAGNG
CCHHHHHHHHHCCCCCCCCCCCCCCEEEEHHHHHHHHHHHHHHEECCCCEEEEEEECCCC
AAGPVIDAIEARLKALGAPVEFIKIHNTPDGTFPKGIPNPLLPECRDDTRKAVIEHGADM
CCCHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCC
GIAFDGDFDRCFLFDEKGKFIEGYYIVGLLAEAFLEKHPGAKIIHDPRLTWNTEAVVTAA
EEEECCCCCEEEEECCCCCEEEHHHHHHHHHHHHHHHCCCCCEEECCCCEECCCEEEEEC
GGTPMMSKTGHAFIKERMRTEDAIYGGEMSAHHYFRDFAYCDSGMIPWLLVAELVCLKGQ
CCCCCCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHCCC
SLGELVRDRMAAFPASGEINSRLAEPAAAIARVEAHFAEEAQAVDRTDGLSMSFADWRFN
HHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEHHEEEE
LRSSNTEPVVRLNVESRGDIPLMEARTRTLLALLNQ
ECCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHCC
>Mature Secondary Structure 
TKLTCFKAYDIRGRLGEELNEDIAWRIGRAYGEYLKPKTIVLGGDVRLTSEVLKMALAK
CCEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHHHHC
GLQDAGVDVLDIGMSGTEEIYFATFHLGVDGGIEVTASHNPMDYNGMKLVREGARPISGD
CHHHCCCCEEEECCCCCCEEEEEEEEEECCCCEEEEECCCCCCCCHHHHHHHCCCCCCCC
TGLRDVQRLAEAGDFPPVNDATRGSYRQISMRDAYIDHLLGYISVNNLTPLKLVFNAGNG
CCHHHHHHHHHCCCCCCCCCCCCCCEEEEHHHHHHHHHHHHHHEECCCCEEEEEEECCCC
AAGPVIDAIEARLKALGAPVEFIKIHNTPDGTFPKGIPNPLLPECRDDTRKAVIEHGADM
CCCHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCC
GIAFDGDFDRCFLFDEKGKFIEGYYIVGLLAEAFLEKHPGAKIIHDPRLTWNTEAVVTAA
EEEECCCCCEEEEECCCCCEEEHHHHHHHHHHHHHHHCCCCCEEECCCCEECCCEEEEEC
GGTPMMSKTGHAFIKERMRTEDAIYGGEMSAHHYFRDFAYCDSGMIPWLLVAELVCLKGQ
CCCCCCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHCCC
SLGELVRDRMAAFPASGEINSRLAEPAAAIARVEAHFAEEAQAVDRTDGLSMSFADWRFN
HHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEHHEEEE
LRSSNTEPVVRLNVESRGDIPLMEARTRTLLALLNQ
ECCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1383393 [H]