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The map label for this gene is wcaE [H]

Identifier: 29141262

GI number: 29141262

Start: 850907

End: 851653

Strand: Direct

Name: wcaE [H]

Synonym: t0761

Alternate gene names: 29141262

Gene position: 850907-851653 (Clockwise)

Preceding gene: 29141261

Following gene: 29141263

Centisome position: 17.76

GC content: 49.13

Gene sequence:

>747_bases
ATGTTTTTAAGCGTCGTTACCGTCGCCTTTCGCAATTACGAAGGGGTGGTAAAAACCTGGCGCTCGCTGCGCAATCTGGC
GCGCGATCCGGGCCTGTCATTTGAATGGATCGTAGTGGATGGCGGTTCGGAGGACGGCACGCGCGAATTTCTGCAAAAAC
GTCACGGTGAGTTTAACCTGCGTTTTATCAGTGAAAAGGATCGCGGTATTTATGATGCCATGAACAAAGGCATTGCCTTG
GCGCAGGGGCGCTATACCCTGTTTCTTAATTCCGGTGATGCGTTCCATGATGATGCCGCGCTGTTTGTCCGCCAACTGGC
GCGGCAAAAGGGCGAGGCGATGTATATTGGCGACGCGCTGCTGGATTTCGGCGGCGGGCATAAAATACGCCGGAAAGCTA
AACCCGGGTATTATATCTACCATAGTTTGCCAGCCAGCCATCAGGCGATATTTTTCCCGACGGCAGCGTTAAAAAAGTAT
CCTTATGATCTGCAATATCATGTCTCTTCAGATTATGCGCTGGCGGCCCGTCTCTATAAAGCAGGCTATCCTTTTCGGCG
AATTAACGGACTGGTATCTGAATTTTCGATGGGCGGCGTGTCAACCTCGAATAATCTGGAATTATGTCAGGATGCAAAAA
ACGTACAGCGAAAAATATTACGTGTTCCAGGGTTTTGGGCGCAATTATCCTATTTGTTACGTCTGAAAACGACGGGTAAA
ACAAAAGCGCGGTATAACAAAGTCTAA

Upstream 100 bases:

>100_bases
GTTTTTTACCGGTTCAATATTTAGCCCGGAATATGCTTTTTTAATCGTCTGTCCGTTCATTTTGCGCAAGGCGTTAAAAA
TTTCATAACGGGGTGGTTGC

Downstream 100 bases:

>100_bases
TCACCGGGAGTTATTATGCAGGATCTTAGCGGCTTTACGGTGCCGAAAGGGTTCCGCGGCGCTAATGCGCTAAAAGTACA
ATTATGGTGGGCGGTACAGG

Product: glycosyl transferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 248; Mature: 248

Protein sequence:

>248_residues
MFLSVVTVAFRNYEGVVKTWRSLRNLARDPGLSFEWIVVDGGSEDGTREFLQKRHGEFNLRFISEKDRGIYDAMNKGIAL
AQGRYTLFLNSGDAFHDDAALFVRQLARQKGEAMYIGDALLDFGGGHKIRRKAKPGYYIYHSLPASHQAIFFPTAALKKY
PYDLQYHVSSDYALAARLYKAGYPFRRINGLVSEFSMGGVSTSNNLELCQDAKNVQRKILRVPGFWAQLSYLLRLKTTGK
TKARYNKV

Sequences:

>Translated_248_residues
MFLSVVTVAFRNYEGVVKTWRSLRNLARDPGLSFEWIVVDGGSEDGTREFLQKRHGEFNLRFISEKDRGIYDAMNKGIAL
AQGRYTLFLNSGDAFHDDAALFVRQLARQKGEAMYIGDALLDFGGGHKIRRKAKPGYYIYHSLPASHQAIFFPTAALKKY
PYDLQYHVSSDYALAARLYKAGYPFRRINGLVSEFSMGGVSTSNNLELCQDAKNVQRKILRVPGFWAQLSYLLRLKTTGK
TKARYNKV
>Mature_248_residues
MFLSVVTVAFRNYEGVVKTWRSLRNLARDPGLSFEWIVVDGGSEDGTREFLQKRHGEFNLRFISEKDRGIYDAMNKGIAL
AQGRYTLFLNSGDAFHDDAALFVRQLARQKGEAMYIGDALLDFGGGHKIRRKAKPGYYIYHSLPASHQAIFFPTAALKKY
PYDLQYHVSSDYALAARLYKAGYPFRRINGLVSEFSMGGVSTSNNLELCQDAKNVQRKILRVPGFWAQLSYLLRLKTTGK
TKARYNKV

Specific function: Slime polysaccharide colanic acid biosynthesis. [C]

COG id: COG0463

COG function: function code M; Glycosyltransferases involved in cell wall biogenesis

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1788368, Length=248, Percent_Identity=71.7741935483871, Blast_Score=378, Evalue=1e-106,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001173 [H]

Pfam domain/function: PF00535 Glycos_transf_2 [H]

EC number: NA

Molecular weight: Translated: 28186; Mature: 28186

Theoretical pI: Translated: 10.28; Mature: 10.28

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFLSVVTVAFRNYEGVVKTWRSLRNLARDPGLSFEWIVVDGGSEDGTREFLQKRHGEFNL
CHHHHEEHHHHCCHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCHHHHHHHHHCCCEEE
RFISEKDRGIYDAMNKGIALAQGRYTLFLNSGDAFHDDAALFVRQLARQKGEAMYIGDAL
EEECCCCCCHHHHHCCCEEEECCEEEEEECCCCCCCCHHHHHHHHHHHHCCCEEEECHHH
LDFGGGHKIRRKAKPGYYIYHSLPASHQAIFFPTAALKKYPYDLQYHVSSDYALAARLYK
HHCCCCCEEHHCCCCCEEEEECCCCCCCEEEEEHHHHHHCCCCEEEEECCCHHHHHHHHH
AGYPFRRINGLVSEFSMGGVSTSNNLELCQDAKNVQRKILRVPGFWAQLSYLLRLKTTGK
CCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHEECCCC
TKARYNKV
CCHHCCCC
>Mature Secondary Structure
MFLSVVTVAFRNYEGVVKTWRSLRNLARDPGLSFEWIVVDGGSEDGTREFLQKRHGEFNL
CHHHHEEHHHHCCHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCHHHHHHHHHCCCEEE
RFISEKDRGIYDAMNKGIALAQGRYTLFLNSGDAFHDDAALFVRQLARQKGEAMYIGDAL
EEECCCCCCHHHHHCCCEEEECCEEEEEECCCCCCCCHHHHHHHHHHHHCCCEEEECHHH
LDFGGGHKIRRKAKPGYYIYHSLPASHQAIFFPTAALKKYPYDLQYHVSSDYALAARLYK
HHCCCCCEEHHCCCCCEEEEECCCCCCCEEEEEHHHHHHCCCCEEEEECCCHHHHHHHHH
AGYPFRRINGLVSEFSMGGVSTSNNLELCQDAKNVQRKILRVPGFWAQLSYLLRLKTTGK
CCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHEECCCC
TKARYNKV
CCHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8759852; 9278503 [H]