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The map label for this gene is wcaF [H]

Identifier: 29141263

GI number: 29141263

Start: 851669

End: 852223

Strand: Direct

Name: wcaF [H]

Synonym: t0762

Alternate gene names: 29141263

Gene position: 851669-852223 (Clockwise)

Preceding gene: 29141262

Following gene: 29141264

Centisome position: 17.77

GC content: 49.73

Gene sequence:

>555_bases
ATGCAGGATCTTAGCGGCTTTACGGTGCCGAAAGGGTTCCGCGGCGCTAATGCGCTAAAAGTACAATTATGGTGGGCGGT
ACAGGCGACATTATTCGCCGGATCGCCGCAAATATTGTATCGCTGGCGAGCCTTTTTGCTGCGTCTGTTTGGCGCCAAAA
TTGGAAAAAATGTGGTTATTCGACCGTCAGTAAAAATTACCTATCCGTGGAAATTAACCGTCGGCGATTATGCCTGGATA
GGCGACGACGCTGTGTTATATACGTTGGGTGAAATTAATATTGGCGCACATGCGGTTATTTCACAAAAAGGTTATTTATG
TACCGGTAGCCATGATTATACCAGCGCCCATTTCGATATTAACGCCGCGCCGATTGTTATTGGCGAAAAATGTTGGCTGG
CGACCGATGTTTTTGTCGCGCCCGGCGTGACGATAGGTCATGGCACCGTCGTCGGCGCGCGTAGCAGCGTATTTAAATCA
TTACCGGCAAATGCGATTTGTCGGGGCAATCCCGCAGTGGTAACGCGCCAGCGCGTTCAGAAAGTTACTCCCTAA

Upstream 100 bases:

>100_bases
TGTTCCAGGGTTTTGGGCGCAATTATCCTATTTGTTACGTCTGAAAACGACGGGTAAAACAAAAGCGCGGTATAACAAAG
TCTAATCACCGGGAGTTATT

Downstream 100 bases:

>100_bases
CGGGACTATTTGAGGAAATGAAAATGTCAAAAGTCGCTCTCATTACTGGCGTAACCGGACAGGATGGGTCTTACCTGGCA
GAATTTCTGCTGGAAAAAGG

Product: colanic acid biosynthesis acetyltransferase WcaF

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 184; Mature: 184

Protein sequence:

>184_residues
MQDLSGFTVPKGFRGANALKVQLWWAVQATLFAGSPQILYRWRAFLLRLFGAKIGKNVVIRPSVKITYPWKLTVGDYAWI
GDDAVLYTLGEINIGAHAVISQKGYLCTGSHDYTSAHFDINAAPIVIGEKCWLATDVFVAPGVTIGHGTVVGARSSVFKS
LPANAICRGNPAVVTRQRVQKVTP

Sequences:

>Translated_184_residues
MQDLSGFTVPKGFRGANALKVQLWWAVQATLFAGSPQILYRWRAFLLRLFGAKIGKNVVIRPSVKITYPWKLTVGDYAWI
GDDAVLYTLGEINIGAHAVISQKGYLCTGSHDYTSAHFDINAAPIVIGEKCWLATDVFVAPGVTIGHGTVVGARSSVFKS
LPANAICRGNPAVVTRQRVQKVTP
>Mature_184_residues
MQDLSGFTVPKGFRGANALKVQLWWAVQATLFAGSPQILYRWRAFLLRLFGAKIGKNVVIRPSVKITYPWKLTVGDYAWI
GDDAVLYTLGEINIGAHAVISQKGYLCTGSHDYTSAHFDINAAPIVIGEKCWLATDVFVAPGVTIGHGTVVGARSSVFKS
LPANAICRGNPAVVTRQRVQKVTP

Specific function: Slime polysaccharide colanic acid biosynthesis. [C]

COG id: COG0110

COG function: function code R; Acetyltransferase (isoleucine patch superfamily)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the transferase hexapeptide repeat family [H]

Homologues:

Organism=Escherichia coli, GI1788367, Length=180, Percent_Identity=86.6666666666667, Blast_Score=329, Evalue=6e-92,
Organism=Escherichia coli, GI1786537, Length=138, Percent_Identity=28.9855072463768, Blast_Score=62, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018357
- InterPro:   IPR011004 [H]

Pfam domain/function: NA

EC number: 2.3.1.- [C]

Molecular weight: Translated: 19971; Mature: 19971

Theoretical pI: Translated: 10.29; Mature: 10.29

Prosite motif: PS00101 HEXAPEP_TRANSFERASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
0.5 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
0.5 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQDLSGFTVPKGFRGANALKVQLWWAVQATLFAGSPQILYRWRAFLLRLFGAKIGKNVVI
CCCCCCCCCCCCCCCCCEEEEEEEEEEEEEEECCCHHHHHHHHHHHHHHHHHHHCCCEEE
RPSVKITYPWKLTVGDYAWIGDDAVLYTLGEINIGAHAVISQKGYLCTGSHDYTSAHFDI
CCCEEEEEEEEEEECCEEEECCCEEEEEEECCCCCCEEEEECCCEEEECCCCCEEEEEEC
NAAPIVIGEKCWLATDVFVAPGVTIGHGTVVGARSSVFKSLPANAICRGNPAVVTRQRVQ
CCCCEEEECCEEEEEEEEECCCEEECCCEEECCHHHHHHHCCCCEEECCCCCEEEHHHHH
KVTP
CCCC
>Mature Secondary Structure
MQDLSGFTVPKGFRGANALKVQLWWAVQATLFAGSPQILYRWRAFLLRLFGAKIGKNVVI
CCCCCCCCCCCCCCCCCEEEEEEEEEEEEEEECCCHHHHHHHHHHHHHHHHHHHCCCEEE
RPSVKITYPWKLTVGDYAWIGDDAVLYTLGEINIGAHAVISQKGYLCTGSHDYTSAHFDI
CCCEEEEEEEEEEECCEEEECCCEEEEEEECCCCCCEEEEECCCEEEECCCCCEEEEEEC
NAAPIVIGEKCWLATDVFVAPGVTIGHGTVVGARSSVFKSLPANAICRGNPAVVTRQRVQ
CCCCEEEECCEEEEEEEEECCCEEECCCEEECCHHHHHHHCCCCEEECCCCCEEEHHHHH
KVTP
CCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8759852; 9097040; 9278503 [H]