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The map label for this gene is pgtE [H]

Identifier: 29140984

GI number: 29140984

Start: 537782

End: 538720

Strand: Direct

Name: pgtE [H]

Synonym: t0463

Alternate gene names: 29140984

Gene position: 537782-538720 (Clockwise)

Preceding gene: 29140983

Following gene: 29140988

Centisome position: 11.22

GC content: 46.43

Gene sequence:

>939_bases
ATGAAAAAACATGCTATTGCAGTAATGATGATCGCCGTATTTTCTGAGTCGGTTTATGCGGAGTCTACCTTATTTATTCC
GGACGTCTCTCCTGAGAGCGTCACGACATCCCTTTCTGTGGGGGTGTTAAACGGTAAATCCAGGGAGCTGGTTTATGATA
CCGACACCGGACGGAAGCTGAGTCAACTGGACTGGAAAATAAAAAATGTCGCCACGTTGCAGGGGGATTTATCATGGGAA
CCCTATTCGTTCATGACGCTGGACGCCCGCGGCTGGACGTCTTTGGCGTCGGGATCGGGTCATATGGTTGACCATGACTG
GATGAGCAGTGAGCAGCCAGGCTGGACCGATCGTTCAATTCATCCGGACACCAGCGCCAACTATGCTAATGAATACGATT
TGAACGTGAAAGGTTGGTTATTGCAGGGAGATAACTACAAGGCGGGCGTAACAGCGGGCTATCAGGAAACTCGTTTTAGC
TGGACGGCAAGAGGCGGGTCTTATATTTATGATAATGGTCGATATATTGGTAACTTTCCTCATGGCGTGCGCGGTATAGG
TTATAGCCAGCGTTTCGAAATGCCCTATATCGGGCTGGCGGGTGATTATCGTATTAATGACTTTGAGTGTAATGTACTGT
TTAAATACAGCGACTGGGTAAATGCGCATGATAATGACGAACACTACATGCGAAAACTTACCTTCCGTGAAAAAACGGAA
AATTCACGATATTATGGCGCGTCTATTGACGCCGGATATTATATTACCAGTAATGCAAAAATCTTTGCTGAGTTCGCTTA
CAGTAAATATGAAGAAGGTAAGGGCGGTACGCAAATCATAGATAAAACCAGCGGTAATACGGCGTATTTTGGTGGCGATG
CCGCAGGTATAGCTAATAATAACTATACGGTTACCGCGGGGTTGCAGTACCGCTTCTAG

Upstream 100 bases:

>100_bases
CAAGTTCTCTGGCGTAATAAATGTACTCTTGTCCGACGATTTGACAAGATGAAAACTTCATCACCTCTCCAGATTACATC
TGAATATGAGGACAAGAGAA

Downstream 100 bases:

>100_bases
ACCACATCGGGATGTCATCGGTCATAACCGGCCGATGACGACTTTTTGCTGAACGTGTGGCATGCCCGGTGATATTGTGT
TTACCTCATATAGATCTGGC

Product: outer membrane protease

Products: Hydrolyzed protein [C]

Alternate protein names: E protein [H]

Number of amino acids: Translated: 312; Mature: 312

Protein sequence:

>312_residues
MKKHAIAVMMIAVFSESVYAESTLFIPDVSPESVTTSLSVGVLNGKSRELVYDTDTGRKLSQLDWKIKNVATLQGDLSWE
PYSFMTLDARGWTSLASGSGHMVDHDWMSSEQPGWTDRSIHPDTSANYANEYDLNVKGWLLQGDNYKAGVTAGYQETRFS
WTARGGSYIYDNGRYIGNFPHGVRGIGYSQRFEMPYIGLAGDYRINDFECNVLFKYSDWVNAHDNDEHYMRKLTFREKTE
NSRYYGASIDAGYYITSNAKIFAEFAYSKYEEGKGGTQIIDKTSGNTAYFGGDAAGIANNNYTVTAGLQYRF

Sequences:

>Translated_312_residues
MKKHAIAVMMIAVFSESVYAESTLFIPDVSPESVTTSLSVGVLNGKSRELVYDTDTGRKLSQLDWKIKNVATLQGDLSWE
PYSFMTLDARGWTSLASGSGHMVDHDWMSSEQPGWTDRSIHPDTSANYANEYDLNVKGWLLQGDNYKAGVTAGYQETRFS
WTARGGSYIYDNGRYIGNFPHGVRGIGYSQRFEMPYIGLAGDYRINDFECNVLFKYSDWVNAHDNDEHYMRKLTFREKTE
NSRYYGASIDAGYYITSNAKIFAEFAYSKYEEGKGGTQIIDKTSGNTAYFGGDAAGIANNNYTVTAGLQYRF
>Mature_312_residues
MKKHAIAVMMIAVFSESVYAESTLFIPDVSPESVTTSLSVGVLNGKSRELVYDTDTGRKLSQLDWKIKNVATLQGDLSWE
PYSFMTLDARGWTSLASGSGHMVDHDWMSSEQPGWTDRSIHPDTSANYANEYDLNVKGWLLQGDNYKAGVTAGYQETRFS
WTARGGSYIYDNGRYIGNFPHGVRGIGYSQRFEMPYIGLAGDYRINDFECNVLFKYSDWVNAHDNDEHYMRKLTFREKTE
NSRYYGASIDAGYYITSNAKIFAEFAYSKYEEGKGGTQIIDKTSGNTAYFGGDAAGIANNNYTVTAGLQYRF

Specific function: Protease that can cleave T7 RNA polymerase. Specific for paired basic residues [H]

COG id: COG4571

COG function: function code M; Outer membrane protease

Gene ontology:

Cell location: Cell outer membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase A26 family [H]

Homologues:

Organism=Escherichia coli, GI1786777, Length=320, Percent_Identity=46.5625, Blast_Score=298, Evalue=4e-82,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020080
- InterPro:   IPR000036
- InterPro:   IPR020079 [H]

Pfam domain/function: PF01278 Omptin [H]

EC number: 3.4.21.87 [C]

Molecular weight: Translated: 35070; Mature: 35070

Theoretical pI: Translated: 5.38; Mature: 5.38

Prosite motif: PS00018 EF_HAND_1 ; PS00834 OMPTIN_1 ; PS00835 OMPTIN_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKHAIAVMMIAVFSESVYAESTLFIPDVSPESVTTSLSVGVLNGKSRELVYDTDTGRKL
CCCHHHHEEEEEHHHCCCCCCEEEEECCCCCCCEEEEEEEEEECCCCCEEEEECCCCCCH
SQLDWKIKNVATLQGDLSWEPYSFMTLDARGWTSLASGSGHMVDHDWMSSEQPGWTDRSI
HHHCEEEEEEEEEECCCCCCCEEEEEEECCCCEEECCCCCCEEECCCCCCCCCCCCCCCC
HPDTSANYANEYDLNVKGWLLQGDNYKAGVTAGYQETRFSWTARGGSYIYDNGRYIGNFP
CCCCCCCCCCEECCCEEEEEEECCCEEECEECCCCCCEEEEEECCCCEEEECCEEEECCC
HGVRGIGYSQRFEMPYIGLAGDYRINDFECNVLFKYSDWVNAHDNDEHYMRKLTFREKTE
CCCCCCCCCCCCCCCEEECCCCEEECEEEEEEEEEECCCCCCCCCHHHHHHHHHHHHCCC
NSRYYGASIDAGYYITSNAKIFAEFAYSKYEEGKGGTQIIDKTSGNTAYFGGDAAGIANN
CCEEEEEECCCCEEEECCCEEEEEEHHHHHHCCCCCCEEEECCCCCEEEECCCCCCCCCC
NYTVTAGLQYRF
CEEEEEEEEECC
>Mature Secondary Structure
MKKHAIAVMMIAVFSESVYAESTLFIPDVSPESVTTSLSVGVLNGKSRELVYDTDTGRKL
CCCHHHHEEEEEHHHCCCCCCEEEEECCCCCCCEEEEEEEEEECCCCCEEEEECCCCCCH
SQLDWKIKNVATLQGDLSWEPYSFMTLDARGWTSLASGSGHMVDHDWMSSEQPGWTDRSI
HHHCEEEEEEEEEECCCCCCCEEEEEEECCCCEEECCCCCCEEECCCCCCCCCCCCCCCC
HPDTSANYANEYDLNVKGWLLQGDNYKAGVTAGYQETRFSWTARGGSYIYDNGRYIGNFP
CCCCCCCCCCEECCCEEEEEEECCCEEECEECCCCCCEEEEEECCCCEEEECCEEEECCC
HGVRGIGYSQRFEMPYIGLAGDYRINDFECNVLFKYSDWVNAHDNDEHYMRKLTFREKTE
CCCCCCCCCCCCCCCEEECCCCEEECEEEEEEEEEECCCCCCCCCHHHHHHHHHHHHCCC
NSRYYGASIDAGYYITSNAKIFAEFAYSKYEEGKGGTQIIDKTSGNTAYFGGDAAGIANN
CCEEEEEECCCCEEEECCCEEEEEEHHHHHHCCCCCCEEEECCCCCEEEECCCCCCCCCC
NYTVTAGLQYRF
CEEEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: EDTA; Tosyl-L-Lys chloromethyl ketone [C]

Metal ions: NA

Kcat value (1/min): 40 [C]

Specific activity: 58.7

Km value (mM): 0.0036 {plasminogen}} 0.000058 {plasminogen}} [C]

Substrates: Protein; H2O [C]

Specific reaction: Protein + H2O = hydrolyzed protein [C]

General reaction: Peptide bond hydrolysis [C]

Inhibitor: Arginine; Benzamidine; Co2+; Cu2+; Diisopropyl fluorophosphate; Fe2+; NaCl; Tosyl -L-phenyl alaninechloromethyl ketone; Zn2+ [C]

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 3023201; 10869088; 11677609; 2651422 [H]