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The map label for this gene is vacJ [H]

Identifier: 29140988

GI number: 29140988

Start: 545225

End: 545980

Strand: Direct

Name: vacJ [H]

Synonym: t0472

Alternate gene names: 29140988

Gene position: 545225-545980 (Clockwise)

Preceding gene: 29140984

Following gene: 29140990

Centisome position: 11.38

GC content: 53.84

Gene sequence:

>756_bases
ATGAAGCTTCGCCTGTCGGCGCTGGCGCTGGGAACCACACTGCTGGTCGGGTGTGCGAGTTCCGGTACAGAACAGCAGGG
GCGTTCAGACCCATTTGAAGGGTTTAACCGCACCATGTACAACTTCAACTTTAATGTGCTGGACCCGTATGTTGTTCGGC
CGGTCGCGGTCGCCTGGCGTGATTATGTTCCACAGCCTGCGCGTAACGGTTTAAGTAATTTCACCGGCAACCTCGAAGAA
CCGGCGATCATGGTGAACTATTTCCTGCAGGGCGATCCTTATCAGGGTATGGTGCATTTCACCCGTTTCTTCCTGAATAC
CTTATTAGGGATGGGCGGCTTTATTGACGTCGCGGGAATGGCGAATCCTAAGCTGCAGCGCGTTGAACCGCATCGCTTTG
GCAGTACACTGGGCCATTATGGCGTGGGGTATGGGCCTTATATGCAACTTCCGTTCTACGGCAGCTTCACGCTGCGTGAA
GATGGCGGGGATATGGCGGATACCTTGTATCCGGTGCTCTCGTGGTTGACCTGGCCAATGTCCATAGGAAAATGGACTAT
CGAAGGGATAGAAACCCGCGCGCAGTTGCTGGACTCCGATGGTTTGCTGCGCCAGTCTTCCGATCCTTATATTATGGTGC
GCGAGGCATACTTCCAGCGTCATGACTTTATCGCCAATGGCGGAAAACTCAAGCCGCAGGAAAACCCGAACGCGCAAGCG
ATTCAGGACGAACTCAAAGAGATCGACTCGGAATAA

Upstream 100 bases:

>100_bases
TGACGTGCTGTTAAAATCGCTCACACCTAAACAGGCGGATACGGTAGCGTTCCGTCATGGATGGCAATCAGCGATAGCCA
TAATTCACAGGGAGACATCT

Downstream 100 bases:

>100_bases
ACGGCAGTAAATAAAAAGGTGAGCGCAATGCTCACCTTTTTGATCCGTTTCAGAAAGAGATTAGAACGCGTAGTTAAAGT
TAGTACCGAACAGCCAGGCT

Product: VacJ lipoprotein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 251; Mature: 251

Protein sequence:

>251_residues
MKLRLSALALGTTLLVGCASSGTEQQGRSDPFEGFNRTMYNFNFNVLDPYVVRPVAVAWRDYVPQPARNGLSNFTGNLEE
PAIMVNYFLQGDPYQGMVHFTRFFLNTLLGMGGFIDVAGMANPKLQRVEPHRFGSTLGHYGVGYGPYMQLPFYGSFTLRE
DGGDMADTLYPVLSWLTWPMSIGKWTIEGIETRAQLLDSDGLLRQSSDPYIMVREAYFQRHDFIANGGKLKPQENPNAQA
IQDELKEIDSE

Sequences:

>Translated_251_residues
MKLRLSALALGTTLLVGCASSGTEQQGRSDPFEGFNRTMYNFNFNVLDPYVVRPVAVAWRDYVPQPARNGLSNFTGNLEE
PAIMVNYFLQGDPYQGMVHFTRFFLNTLLGMGGFIDVAGMANPKLQRVEPHRFGSTLGHYGVGYGPYMQLPFYGSFTLRE
DGGDMADTLYPVLSWLTWPMSIGKWTIEGIETRAQLLDSDGLLRQSSDPYIMVREAYFQRHDFIANGGKLKPQENPNAQA
IQDELKEIDSE
>Mature_251_residues
MKLRLSALALGTTLLVGCASSGTEQQGRSDPFEGFNRTMYNFNFNVLDPYVVRPVAVAWRDYVPQPARNGLSNFTGNLEE
PAIMVNYFLQGDPYQGMVHFTRFFLNTLLGMGGFIDVAGMANPKLQRVEPHRFGSTLGHYGVGYGPYMQLPFYGSFTLRE
DGGDMADTLYPVLSWLTWPMSIGKWTIEGIETRAQLLDSDGLLRQSSDPYIMVREAYFQRHDFIANGGKLKPQENPNAQA
IQDELKEIDSE

Specific function: Actively prevents phospholipid accumulation at the cell surface. Probably maintains lipid asymmetry in the outer membrane by retrograde trafficking of phospholipids from the outer membrane to the inner membrane [H]

COG id: COG2853

COG function: function code M; Surface lipoprotein

Gene ontology:

Cell location: Cell outer membrane; Lipid-anchor (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mlaA family [H]

Homologues:

Organism=Escherichia coli, GI1788688, Length=251, Percent_Identity=94.0239043824701, Blast_Score=500, Evalue=1e-143,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007428 [H]

Pfam domain/function: PF04333 VacJ [H]

EC number: NA

Molecular weight: Translated: 28185; Mature: 28185

Theoretical pI: Translated: 4.90; Mature: 4.90

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLRLSALALGTTLLVGCASSGTEQQGRSDPFEGFNRTMYNFNFNVLDPYVVRPVAVAWR
CCEEHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHCCEEEEECCCCCCHHHHHHHHHHHH
DYVPQPARNGLSNFTGNLEEPAIMVNYFLQGDPYQGMVHFTRFFLNTLLGMGGFIDVAGM
HHCCCHHHCCHHHHCCCCCCCEEEEEEEECCCCCCHHHHHHHHHHHHHHCCCCCEEECCC
ANPKLQRVEPHRFGSTLGHYGVGYGPYMQLPFYGSFTLREDGGDMADTLYPVLSWLTWPM
CCCCHHCCCCHHHHHHHHCCCCCCCCEEECCCCCCEEEECCCCCHHHHHHHHHHHHHHHH
SIGKWTIEGIETRAQLLDSDGLLRQSSDPYIMVREAYFQRHDFIANGGKLKPQENPNAQA
HCCCEEHHHHHHHHHHHCCCCCCCCCCCCEEEEHHHHHHHHHHHCCCCCCCCCCCCCHHH
IQDELKEIDSE
HHHHHHHHCCC
>Mature Secondary Structure
MKLRLSALALGTTLLVGCASSGTEQQGRSDPFEGFNRTMYNFNFNVLDPYVVRPVAVAWR
CCEEHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHCCEEEEECCCCCCHHHHHHHHHHHH
DYVPQPARNGLSNFTGNLEEPAIMVNYFLQGDPYQGMVHFTRFFLNTLLGMGGFIDVAGM
HHCCCHHHCCHHHHCCCCCCCEEEEEEEECCCCCCHHHHHHHHHHHHHHCCCCCEEECCC
ANPKLQRVEPHRFGSTLGHYGVGYGPYMQLPFYGSFTLREDGGDMADTLYPVLSWLTWPM
CCCCHHCCCCHHHHHHHHCCCCCCCCEEECCCCCCEEEECCCCCHHHHHHHHHHHHHHHH
SIGKWTIEGIETRAQLLDSDGLLRQSSDPYIMVREAYFQRHDFIANGGKLKPQENPNAQA
HCCCEEHHHHHHHHHHHCCCCCCCCCCCCEEEEHHHHHHHHHHHCCCCCCCCCCCCCHHH
IQDELKEIDSE
HHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 9205837; 9278503 [H]