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The map label for this gene is ptsJ [H]

Identifier: 29140948

GI number: 29140948

Start: 494098

End: 495390

Strand: Reverse

Name: ptsJ [H]

Synonym: t0421

Alternate gene names: 29140948

Gene position: 495390-494098 (Counterclockwise)

Preceding gene: 29140951

Following gene: 29140947

Centisome position: 10.34

GC content: 59.01

Gene sequence:

>1293_bases
ATGATCGACGGAAAAACCGCTAACGAAATTTTTGACAGTATCCGTCAACACATTATCGCCGGCACGTTACGGGCGAAGGA
TTCGCTCCCGCCGGTACGGGAGCTGGCAAGCGAATTAAAGGTGAACCGCAATACCGTCGCAGCGGCGTATAAACGGCTGA
TAACGGCGGGACTGGCGCAAAGTTTAGGCCGCAATGGGACGGTGATTAAAGGCAGTCCGTCTCCCGTAGCGTTGGAAGGC
GGCGATCCGCATACGCCTCTGCACGACCTTTCCGGCGGCAATCCCGATCCGCAGCGTCTGCCTGACCTTAGCCGTTATTT
TGCCCGTCTCAGCCGCACGCCGCATCTCTACGGCGATGCGCCCGTTTCCCCTGAACTACACGCCTGGGCGGCGCGCTGGC
TACGGGATGCCACCCCCGTCGCAGGCGAAATTGATATCACCAGCGGAGCTATCGACGCTATCGAAAGACTGCTTTGCGCG
CATCTGCTGCCGGGCGACAGCGTTGCGGTGGAAGATCCCTGTTTTTTAAGCAGTATCAATATGCTGCGCTATGCCGGTTT
TAGCGCCAGCCCGGTCAGTGTGGATAGCGAAGGAATGCAGCCTGAAAAGCTGGAGCAGGCGCTAAACCAGGGCGCGCGGG
CAGTGATCTTAACGCCGCGCGCACACAACCCTACCGGCTGTAGCCTGAGCGCTCGCCGTGCCGCTGCGCTGCAAAATATA
CTGGCGCGTTACCCTCAGGTACTGGTCATTATCGACGATCACTTCGCCCTGCTTTCTTCGTCGCCCTGGCAGCCGGTTAT
TGCGCAGACAACGCAACACTGGGCGGTGATACGCTCCGTTTCCAAAGCCCTCGGCCCCGATTTACGTCTTGCTATTGTCG
CCAGCGACAGCTCGACCTCGGCAAAACTTCGGCTGCGGCTAAATGCCGGTAGTCAGTGGGTCAGCCATCTGTTACAGGAT
CTGGTCTACGCCTGCCTGACCGACCCTGAGTATCAGCACAGGCTGGCGCAAACGCGGCTGTTTTACGCCACCCGGCAACA
AAAACTGGCCCGCGCCCTACAGCAATATGGCATCGCCATTTCTCCCGGCGACGGCGTGAATGCCTGGCTCCCGCTGGACA
CCCATAGCCAGGCTACCGCGTTTACGCTGGCAAAATCCGGCTGGCTGGTACGTGAGGGCGAAGCCTTTGGCGTAAGCGCG
CCGTCTCACGGTCTACGTATCACGCTGTCGACATTAAATGACGGCGAGATCAACACGCTGGCGGCTGATATCCATCAGGC
GCTAAATCGCTAA

Upstream 100 bases:

>100_bases
ATCACTCTCTTGTCCCATAAAATTTTCCTCTCGCTGGACAGCAGGCTACCGCCTCATTATCATTGTCATATGCTAAAATT
CATTTTGTCATAGGTCAATA

Downstream 100 bases:

>100_bases
CAGGAGAAAATAACGTGCGAGTTCATTTTGTTATCCATGAGTCGTTTGAATCCGCTGGCGCTTATCTGAAATGGGCTGAA
GATCGCGGTTATACCATTTC

Product: transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 430; Mature: 430

Protein sequence:

>430_residues
MIDGKTANEIFDSIRQHIIAGTLRAKDSLPPVRELASELKVNRNTVAAAYKRLITAGLAQSLGRNGTVIKGSPSPVALEG
GDPHTPLHDLSGGNPDPQRLPDLSRYFARLSRTPHLYGDAPVSPELHAWAARWLRDATPVAGEIDITSGAIDAIERLLCA
HLLPGDSVAVEDPCFLSSINMLRYAGFSASPVSVDSEGMQPEKLEQALNQGARAVILTPRAHNPTGCSLSARRAAALQNI
LARYPQVLVIIDDHFALLSSSPWQPVIAQTTQHWAVIRSVSKALGPDLRLAIVASDSSTSAKLRLRLNAGSQWVSHLLQD
LVYACLTDPEYQHRLAQTRLFYATRQQKLARALQQYGIAISPGDGVNAWLPLDTHSQATAFTLAKSGWLVREGEAFGVSA
PSHGLRITLSTLNDGEINTLAADIHQALNR

Sequences:

>Translated_430_residues
MIDGKTANEIFDSIRQHIIAGTLRAKDSLPPVRELASELKVNRNTVAAAYKRLITAGLAQSLGRNGTVIKGSPSPVALEG
GDPHTPLHDLSGGNPDPQRLPDLSRYFARLSRTPHLYGDAPVSPELHAWAARWLRDATPVAGEIDITSGAIDAIERLLCA
HLLPGDSVAVEDPCFLSSINMLRYAGFSASPVSVDSEGMQPEKLEQALNQGARAVILTPRAHNPTGCSLSARRAAALQNI
LARYPQVLVIIDDHFALLSSSPWQPVIAQTTQHWAVIRSVSKALGPDLRLAIVASDSSTSAKLRLRLNAGSQWVSHLLQD
LVYACLTDPEYQHRLAQTRLFYATRQQKLARALQQYGIAISPGDGVNAWLPLDTHSQATAFTLAKSGWLVREGEAFGVSA
PSHGLRITLSTLNDGEINTLAADIHQALNR
>Mature_430_residues
MIDGKTANEIFDSIRQHIIAGTLRAKDSLPPVRELASELKVNRNTVAAAYKRLITAGLAQSLGRNGTVIKGSPSPVALEG
GDPHTPLHDLSGGNPDPQRLPDLSRYFARLSRTPHLYGDAPVSPELHAWAARWLRDATPVAGEIDITSGAIDAIERLLCA
HLLPGDSVAVEDPCFLSSINMLRYAGFSASPVSVDSEGMQPEKLEQALNQGARAVILTPRAHNPTGCSLSARRAAALQNI
LARYPQVLVIIDDHFALLSSSPWQPVIAQTTQHWAVIRSVSKALGPDLRLAIVASDSSTSAKLRLRLNAGSQWVSHLLQD
LVYACLTDPEYQHRLAQTRLFYATRQQKLARALQQYGIAISPGDGVNAWLPLDTHSQATAFTLAKSGWLVREGEAFGVSA
PSHGLRITLSTLNDGEINTLAADIHQALNR

Specific function: Putative transcriptional regulator of the ptsHI/crr operon [H]

COG id: COG1167

COG function: function code KE; Transcriptional regulators containing a DNA-binding HTH domain and an aminotransferase domain (MocR family) and their eukaryotic orthologs

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH gntR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1787710, Length=418, Percent_Identity=28.4688995215311, Blast_Score=94, Evalue=3e-20,
Organism=Escherichia coli, GI1790797, Length=319, Percent_Identity=30.0940438871473, Blast_Score=85, Evalue=8e-18,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004839
- InterPro:   IPR000524
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00155 Aminotran_1_2; PF00392 GntR [H]

EC number: NA

Molecular weight: Translated: 46431; Mature: 46431

Theoretical pI: Translated: 8.06; Mature: 8.06

Prosite motif: PS50949 HTH_GNTR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
0.7 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIDGKTANEIFDSIRQHIIAGTLRAKDSLPPVRELASELKVNRNTVAAAYKRLITAGLAQ
CCCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHH
SLGRNGTVIKGSPSPVALEGGDPHTPLHDLSGGNPDPQRLPDLSRYFARLSRTPHLYGDA
HHCCCCCEEECCCCCEEECCCCCCCCCHHCCCCCCCHHHCHHHHHHHHHHCCCCCCCCCC
PVSPELHAWAARWLRDATPVAGEIDITSGAIDAIERLLCAHLLPGDSVAVEDPCFLSSIN
CCCCHHHHHHHHHHHCCCCCCCEEECCCHHHHHHHHHHHHHCCCCCCEEECCCHHHHHHH
MLRYAGFSASPVSVDSEGMQPEKLEQALNQGARAVILTPRAHNPTGCSLSARRAAALQNI
HHHHCCCCCCCCCCCCCCCCHHHHHHHHCCCCEEEEECCCCCCCCCCCCHHHHHHHHHHH
LARYPQVLVIIDDHFALLSSSPWQPVIAQTTQHWAVIRSVSKALGPDLRLAIVASDSSTS
HHHCCEEEEEECCCHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCC
AKLRLRLNAGSQWVSHLLQDLVYACLTDPEYQHRLAQTRLFYATRQQKLARALQQYGIAI
EEEEEEECCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEE
SPGDGVNAWLPLDTHSQATAFTLAKSGWLVREGEAFGVSAPSHGLRITLSTLNDGEINTL
CCCCCCCEEEECCCCCCHHEEEEECCCCEEECCCEECCCCCCCCEEEEEEECCCCCHHHH
AADIHQALNR
HHHHHHHHCC
>Mature Secondary Structure
MIDGKTANEIFDSIRQHIIAGTLRAKDSLPPVRELASELKVNRNTVAAAYKRLITAGLAQ
CCCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHH
SLGRNGTVIKGSPSPVALEGGDPHTPLHDLSGGNPDPQRLPDLSRYFARLSRTPHLYGDA
HHCCCCCEEECCCCCEEECCCCCCCCCHHCCCCCCCHHHCHHHHHHHHHHCCCCCCCCCC
PVSPELHAWAARWLRDATPVAGEIDITSGAIDAIERLLCAHLLPGDSVAVEDPCFLSSIN
CCCCHHHHHHHHHHHCCCCCCCEEECCCHHHHHHHHHHHHHCCCCCCEEECCCHHHHHHH
MLRYAGFSASPVSVDSEGMQPEKLEQALNQGARAVILTPRAHNPTGCSLSARRAAALQNI
HHHHCCCCCCCCCCCCCCCCHHHHHHHHCCCCEEEEECCCCCCCCCCCCHHHHHHHHHHH
LARYPQVLVIIDDHFALLSSSPWQPVIAQTTQHWAVIRSVSKALGPDLRLAIVASDSSTS
HHHCCEEEEEECCCHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCC
AKLRLRLNAGSQWVSHLLQDLVYACLTDPEYQHRLAQTRLFYATRQQKLARALQQYGIAI
EEEEEEECCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEE
SPGDGVNAWLPLDTHSQATAFTLAKSGWLVREGEAFGVSAPSHGLRITLSTLNDGEINTL
CCCCCCCEEEECCCCCCHHEEEEECCCCEEECCCEECCCCCCCCEEEEEEECCCCCHHHH
AADIHQALNR
HHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7612925; 11677609 [H]