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The map label for this gene is kgtP [H]

Identifier: 29140791

GI number: 29140791

Start: 293573

End: 294874

Strand: Direct

Name: kgtP [H]

Synonym: t0256

Alternate gene names: 29140791

Gene position: 293573-294874 (Clockwise)

Preceding gene: 29140790

Following gene: 29140797

Centisome position: 6.13

GC content: 51.77

Gene sequence:

>1302_bases
ATGGCAGAAATTATTCAGCGTATAGATAAAAACAACGCTGAGGCTCCGGAGATGCGGCGACACATATGGGCTATCGTGGG
GGCCTCTTCCGGAAACTTAGTGGAATGGTTTGATTTCTACGTCTACTCTTTCTGCTCGCTCTATTTTGCACATATCTTCT
TTCCTTCTAGTAATACCACTACTCAGCTTTTGCAAACGGCAGGCGTCTTTGCCGCCGGTTTTTTAATGCGGTCTATCGGT
GGTTGGCTGTTTGGACGCATTGCCGATCGACGCGGGCGCAAAGCCTCAATGTTGATTTCGGTGTGCATGATGTGCATGGG
GTCGCTGGTGATTGCCTGCTTACCTGGTTACGATACCATCGGCACCTGGGCACCGGCGCTGCTACTTATTGCAAGGTTAT
TTCAGGGGCTCTCGGTAGGGGGAGAGTATGGGACCAGCGCGACATATATGAGTGAAGTTGCGCTTGAAGGTCGTAAGGGA
TTTTTCGCGTCGTTTCAGTACGTTACGCTTATCGGTGGCCAACTACTGGCATTGCTGGTCGTTGTGATTTTGCAGCAGGT
TCTGGAGGATGCTGAGCTTCGTGCCTGGGGATGGCGTATTCCCTTTGCGCTGGGCGCCGTGCTGGCGGTTGTGGCGTTAT
GGCTACGTCGCCAGCTGGATGAAACCTCGAAGCATGAGACTCGCGCATTAAAAGAAGCCGGTTCGTTGAAAGGTTTGTGG
CGTAATCGCAAAGCCTTTTTGATGGTGCTTGGTTTCACCGCGGCAGGCTCGCTGTGCTTTTATACCTTCACCACCTATAT
GCAGAAATATCTGGTAAATACAGCAGGTATGCATGCCAATGTCGCCAGCGGCATTATGACGGCTGCGCTGTGTGTTTTCA
TGTTGGTGCAGCCGTTATTCGGCGCGTTATCCGATAAGATTGGCAGGCGCACCTCTATGCTATGTTTTGGCGCACTCGCG
ACGCTTTTCACCGTTCCAATTCTCTCCGCGTTGCAGAATGTGACGTCGCCTTATGCCGCATTTGCTTTAGTGATGTGCGC
GCTATTGATTGTGAGTTTCTATACCTCGATTAGCGGGATCCTGAAAGCGGAGATGTTTCCGGCGCAGGTTCGCGCCCTGG
GCGTCGGTTTATCTTACGCGGTAGCAAACGCGTTATTTGGCGGCTCTGCGGAATATGTCGCGCTGTCGCTAAAATCTGTC
GGTATGGAGAGTTCGTTCTTCTGGTATGTTACCGCGATGGCCGTTCTGGCTTTCCTGGTTTCGTTGATGTTGCACCGTAA
AGGAAAGGGCTTACGCCTTTAG

Upstream 100 bases:

>100_bases
TTAGCAGACAAATAATTAACACTTTATTTACTTTTTAGTCTCGCGTAATCTGAATCTCCAAAATAAAAGGCCGATTAAGC
CTGGCAACAAGGAGAACAGT

Downstream 100 bases:

>100_bases
TATCGCGCCATTTGCCAGATAGCGTAACCGGTTGTCGCGCCAGCGACATCCCAGACAAAATCTTTCCAGCTCCAGCCGCT
GCCTTCCGGGCGGCTGTCCC

Product: alpha-ketoglutarate transporter

Products: Proton [Cytoplasm]; 2-oxoglutarate [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 433; Mature: 432

Protein sequence:

>433_residues
MAEIIQRIDKNNAEAPEMRRHIWAIVGASSGNLVEWFDFYVYSFCSLYFAHIFFPSSNTTTQLLQTAGVFAAGFLMRSIG
GWLFGRIADRRGRKASMLISVCMMCMGSLVIACLPGYDTIGTWAPALLLIARLFQGLSVGGEYGTSATYMSEVALEGRKG
FFASFQYVTLIGGQLLALLVVVILQQVLEDAELRAWGWRIPFALGAVLAVVALWLRRQLDETSKHETRALKEAGSLKGLW
RNRKAFLMVLGFTAAGSLCFYTFTTYMQKYLVNTAGMHANVASGIMTAALCVFMLVQPLFGALSDKIGRRTSMLCFGALA
TLFTVPILSALQNVTSPYAAFALVMCALLIVSFYTSISGILKAEMFPAQVRALGVGLSYAVANALFGGSAEYVALSLKSV
GMESSFFWYVTAMAVLAFLVSLMLHRKGKGLRL

Sequences:

>Translated_433_residues
MAEIIQRIDKNNAEAPEMRRHIWAIVGASSGNLVEWFDFYVYSFCSLYFAHIFFPSSNTTTQLLQTAGVFAAGFLMRSIG
GWLFGRIADRRGRKASMLISVCMMCMGSLVIACLPGYDTIGTWAPALLLIARLFQGLSVGGEYGTSATYMSEVALEGRKG
FFASFQYVTLIGGQLLALLVVVILQQVLEDAELRAWGWRIPFALGAVLAVVALWLRRQLDETSKHETRALKEAGSLKGLW
RNRKAFLMVLGFTAAGSLCFYTFTTYMQKYLVNTAGMHANVASGIMTAALCVFMLVQPLFGALSDKIGRRTSMLCFGALA
TLFTVPILSALQNVTSPYAAFALVMCALLIVSFYTSISGILKAEMFPAQVRALGVGLSYAVANALFGGSAEYVALSLKSV
GMESSFFWYVTAMAVLAFLVSLMLHRKGKGLRL
>Mature_432_residues
AEIIQRIDKNNAEAPEMRRHIWAIVGASSGNLVEWFDFYVYSFCSLYFAHIFFPSSNTTTQLLQTAGVFAAGFLMRSIGG
WLFGRIADRRGRKASMLISVCMMCMGSLVIACLPGYDTIGTWAPALLLIARLFQGLSVGGEYGTSATYMSEVALEGRKGF
FASFQYVTLIGGQLLALLVVVILQQVLEDAELRAWGWRIPFALGAVLAVVALWLRRQLDETSKHETRALKEAGSLKGLWR
NRKAFLMVLGFTAAGSLCFYTFTTYMQKYLVNTAGMHANVASGIMTAALCVFMLVQPLFGALSDKIGRRTSMLCFGALAT
LFTVPILSALQNVTSPYAAFALVMCALLIVSFYTSISGILKAEMFPAQVRALGVGLSYAVANALFGGSAEYVALSLKSVG
MESSFFWYVTAMAVLAFLVSLMLHRKGKGLRL

Specific function: Uptake of alpha-ketoglutarate across the boundary membrane with the concomitant import of a cation (symport system) [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family [H]

Homologues:

Organism=Escherichia coli, GI1788942, Length=433, Percent_Identity=88.2217090069284, Blast_Score=740, Evalue=0.0,
Organism=Escherichia coli, GI1790550, Length=361, Percent_Identity=32.409972299169, Blast_Score=206, Evalue=2e-54,
Organism=Escherichia coli, GI1788292, Length=403, Percent_Identity=31.2655086848635, Blast_Score=187, Evalue=8e-49,
Organism=Escherichia coli, GI1789941, Length=429, Percent_Identity=32.1678321678322, Blast_Score=174, Evalue=8e-45,
Organism=Escherichia coli, GI87082231, Length=175, Percent_Identity=32.5714285714286, Blast_Score=81, Evalue=1e-16,
Organism=Escherichia coli, GI87082404, Length=374, Percent_Identity=23.7967914438503, Blast_Score=75, Evalue=1e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004736
- InterPro:   IPR020846
- InterPro:   IPR016196
- InterPro:   IPR005828
- InterPro:   IPR005829 [H]

Pfam domain/function: PF00083 Sugar_tr [H]

EC number: NA

Molecular weight: Translated: 47267; Mature: 47136

Theoretical pI: Translated: 9.63; Mature: 9.63

Prosite motif: PS50850 MFS ; PS00217 SUGAR_TRANSPORT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
4.4 %Met     (Translated Protein)
6.2 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
4.2 %Met     (Mature Protein)
6.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAEIIQRIDKNNAEAPEMRRHIWAIVGASSGNLVEWFDFYVYSFCSLYFAHIFFPSSNTT
CHHHHHHHCCCCCCCHHHHHHHHHHEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCH
TQLLQTAGVFAAGFLMRSIGGWLFGRIADRRGRKASMLISVCMMCMGSLVIACLPGYDTI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCCH
GTWAPALLLIARLFQGLSVGGEYGTSATYMSEVALEGRKGFFASFQYVTLIGGQLLALLV
HHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHH
VVILQQVLEDAELRAWGWRIPFALGAVLAVVALWLRRQLDETSKHETRALKEAGSLKGLW
HHHHHHHHCCCHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RNRKAFLMVLGFTAAGSLCFYTFTTYMQKYLVNTAGMHANVASGIMTAALCVFMLVQPLF
HCCHHHEEEEEHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHH
GALSDKIGRRTSMLCFGALATLFTVPILSALQNVTSPYAAFALVMCALLIVSFYTSISGI
HHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHH
LKAEMFPAQVRALGVGLSYAVANALFGGSAEYVALSLKSVGMESSFFWYVTAMAVLAFLV
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHH
SLMLHRKGKGLRL
HHHHHCCCCCCCC
>Mature Secondary Structure 
AEIIQRIDKNNAEAPEMRRHIWAIVGASSGNLVEWFDFYVYSFCSLYFAHIFFPSSNTT
HHHHHHHCCCCCCCHHHHHHHHHHEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCH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HHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; 2-oxoglutarate [Periplasm] [C]

Specific reaction: Proton [Periplasm] + 2-oxoglutarate [Periplasm] = Proton [Cytoplasm] + 2-oxoglutarate [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2118499; 9205837; 9278503; 1709493; 2190187; 2053984; 8419306 [H]