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The map label for this gene is yfiF [H]

Identifier: 29140797

GI number: 29140797

Start: 300799

End: 301836

Strand: Direct

Name: yfiF [H]

Synonym: t0262

Alternate gene names: 29140797

Gene position: 300799-301836 (Clockwise)

Preceding gene: 29140791

Following gene: 29140799

Centisome position: 6.28

GC content: 57.03

Gene sequence:

>1038_bases
ATGAACGATGAATTAAAAAACAAAAGCGGCAAAGTCAAAGTGATGTATGTCCGTAGTGATGATGACTCTGATAAACGCAC
CCATAACCCGCGTACCGGGAAAGGGGGAGGCCGCCCAGCAAAGTCTCGCACTGACGGTGGACGTCGCCCCGCCCGTGATG
AGCGAAATAATCAGAGCCGTGACCGTAAACATGAAACTTCGCCGTGGCGCACCGTGTCCCGCGCGCCAGGCGATGAAACG
CCTGAGAAAGTCGATCATGGTGGTATTAGCGGTAAAAGTTTTATCGACCCTGAAGTTTTGCGTCGCCAGCGTGCGGAAGA
GACGCGCGTCTACGGCGAAAATGCCTGCCAGGCGCTATTCCAGAGCCGTCCTGACGCCATCGTGCGCGCATGGTTTATCC
AAAGCGTAACGCCGCGTTTTAAAGAGGCGCTGCGCTGGATGGCGGCGAATCGTAAAGCCTACCATGTGGTGGATGAAGCC
GAACTGGCAAAAGCTTCCGGGACTGAGCACCACGGCGGCGTGTGTTTCCTGATTAAAAAGCGTAACGGTACGACCGTTAA
GCAATGGGTAAAGCAGGCGGCGGATCAAGACTGCGTGCTGGCGTTGGAAGATGTCGCTAACCCGCATAATCTGGGCGGCA
TGATGCGTAGCTGCGCGCACTTCGGCGTGAAGGGCGTGGTGGTACAGGATGCAGCGTTGCTGGAGTCCGGCGCGGCGATT
CGTACCGCGGAAGGCGGGGCGGAACATGTCCAGCCAATCACTGGTGAAAGCATTGTTGACGTACTGGATGACTTTCGTCA
GGCGGGGTACACCGTGGTGACGACGTCCAGCGATCGCGGTCAGGCGCTCTTTAGTACGACCCTGCCGGAAAAGATGGTGC
TGGTCTTAGGCCGTGAATATGACTACCTGCCGGAAGCGGCGCGCGAACCCGACGATCTGTGCGTGAAAATTAACGGCACC
GGGAACGTGGAAAGCCTGAATGTGTCGGTTGCGACAGGCGTACTGCTCGCCGAGTGGTGGCGACAGAATAAAGCGTAA

Upstream 100 bases:

>100_bases
TCAATGAGTACAATAGTCGCAAGCTTTTAAACGAAATCATGGCTAACCGGATGCACATCGGGTAATCTTCGCGCTTCGCG
CAGCGCTGGTGGAGAAAAGC

Downstream 100 bases:

>100_bases
GGGCTGCAGCCCGGTGACGCAACGCGCGCCGGGCCAATGATGTACTTTCTACCGCCCGGTCAGACAAGGCGCTTTCGCGC
CGTTATCCGGGCGATAAAAC

Product: methyltransferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 345; Mature: 345

Protein sequence:

>345_residues
MNDELKNKSGKVKVMYVRSDDDSDKRTHNPRTGKGGGRPAKSRTDGGRRPARDERNNQSRDRKHETSPWRTVSRAPGDET
PEKVDHGGISGKSFIDPEVLRRQRAEETRVYGENACQALFQSRPDAIVRAWFIQSVTPRFKEALRWMAANRKAYHVVDEA
ELAKASGTEHHGGVCFLIKKRNGTTVKQWVKQAADQDCVLALEDVANPHNLGGMMRSCAHFGVKGVVVQDAALLESGAAI
RTAEGGAEHVQPITGESIVDVLDDFRQAGYTVVTTSSDRGQALFSTTLPEKMVLVLGREYDYLPEAAREPDDLCVKINGT
GNVESLNVSVATGVLLAEWWRQNKA

Sequences:

>Translated_345_residues
MNDELKNKSGKVKVMYVRSDDDSDKRTHNPRTGKGGGRPAKSRTDGGRRPARDERNNQSRDRKHETSPWRTVSRAPGDET
PEKVDHGGISGKSFIDPEVLRRQRAEETRVYGENACQALFQSRPDAIVRAWFIQSVTPRFKEALRWMAANRKAYHVVDEA
ELAKASGTEHHGGVCFLIKKRNGTTVKQWVKQAADQDCVLALEDVANPHNLGGMMRSCAHFGVKGVVVQDAALLESGAAI
RTAEGGAEHVQPITGESIVDVLDDFRQAGYTVVTTSSDRGQALFSTTLPEKMVLVLGREYDYLPEAAREPDDLCVKINGT
GNVESLNVSVATGVLLAEWWRQNKA
>Mature_345_residues
MNDELKNKSGKVKVMYVRSDDDSDKRTHNPRTGKGGGRPAKSRTDGGRRPARDERNNQSRDRKHETSPWRTVSRAPGDET
PEKVDHGGISGKSFIDPEVLRRQRAEETRVYGENACQALFQSRPDAIVRAWFIQSVTPRFKEALRWMAANRKAYHVVDEA
ELAKASGTEHHGGVCFLIKKRNGTTVKQWVKQAADQDCVLALEDVANPHNLGGMMRSCAHFGVKGVVVQDAALLESGAAI
RTAEGGAEHVQPITGESIVDVLDDFRQAGYTVVTTSSDRGQALFSTTLPEKMVLVLGREYDYLPEAAREPDDLCVKINGT
GNVESLNVSVATGVLLAEWWRQNKA

Specific function: Unknown

COG id: COG0566

COG function: function code J; rRNA methylases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase TrmH family [H]

Homologues:

Organism=Escherichia coli, GI1788935, Length=345, Percent_Identity=87.536231884058, Blast_Score=633, Evalue=0.0,
Organism=Escherichia coli, GI1790623, Length=247, Percent_Identity=27.5303643724696, Blast_Score=79, Evalue=6e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001537
- InterPro:   IPR013123
- InterPro:   IPR016479 [H]

Pfam domain/function: PF00588 SpoU_methylase; PF08032 SpoU_sub_bind [H]

EC number: 2.1.1.- [C]

Molecular weight: Translated: 38078; Mature: 38078

Theoretical pI: Translated: 8.47; Mature: 8.47

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNDELKNKSGKVKVMYVRSDDDSDKRTHNPRTGKGGGRPAKSRTDGGRRPARDERNNQSR
CCCCCCCCCCCEEEEEEECCCCCCHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHH
DRKHETSPWRTVSRAPGDETPEKVDHGGISGKSFIDPEVLRRQRAEETRVYGENACQALF
HHHCCCCCHHHHHCCCCCCCCHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
QSRPDAIVRAWFIQSVTPRFKEALRWMAANRKAYHVVDEAELAKASGTEHHGGVCFLIKK
HCCCHHHHHHHHHHHCCHHHHHHHHHHHHCCCEEEEECHHHHHHHCCCCCCCCEEEEEEC
RNGTTVKQWVKQAADQDCVLALEDVANPHNLGGMMRSCAHFGVKGVVVQDAALLESGAAI
CCCCHHHHHHHHHCCCCCEEEHHHCCCCHHHHHHHHHHHHCCCCEEEEHHHHHHHCCCEE
RTAEGGAEHVQPITGESIVDVLDDFRQAGYTVVTTSSDRGQALFSTTLPEKMVLVLGREY
EECCCCHHHCCCCCCHHHHHHHHHHHHCCCEEEEECCCCCCEEEHHCCCHHHHHHCCCCC
DYLPEAAREPDDLCVKINGTGNVESLNVSVATGVLLAEWWRQNKA
CCCCHHHCCCCCEEEEECCCCCEEEEEEHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MNDELKNKSGKVKVMYVRSDDDSDKRTHNPRTGKGGGRPAKSRTDGGRRPARDERNNQSR
CCCCCCCCCCCEEEEEEECCCCCCHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHH
DRKHETSPWRTVSRAPGDETPEKVDHGGISGKSFIDPEVLRRQRAEETRVYGENACQALF
HHHCCCCCHHHHHCCCCCCCCHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
QSRPDAIVRAWFIQSVTPRFKEALRWMAANRKAYHVVDEAELAKASGTEHHGGVCFLIKK
HCCCHHHHHHHHHHHCCHHHHHHHHHHHHCCCEEEEECHHHHHHHCCCCCCCCEEEEEEC
RNGTTVKQWVKQAADQDCVLALEDVANPHNLGGMMRSCAHFGVKGVVVQDAALLESGAAI
CCCCHHHHHHHHHCCCCCEEEHHHCCCCHHHHHHHHHHHHCCCCEEEEHHHHHHHCCCEE
RTAEGGAEHVQPITGESIVDVLDDFRQAGYTVVTTSSDRGQALFSTTLPEKMVLVLGREY
EECCCCHHHCCCCCCHHHHHHHHHHHHCCCEEEEECCCCCCEEEHHCCCHHHHHHCCCCC
DYLPEAAREPDDLCVKINGTGNVESLNVSVATGVLLAEWWRQNKA
CCCCHHHCCCCCEEEEECCCCCEEEEEEHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]