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The map label for this gene is rof [H]

Identifier: 29140777

GI number: 29140777

Start: 277629

End: 277883

Strand: Reverse

Name: rof [H]

Synonym: t0239

Alternate gene names: 29140777

Gene position: 277883-277629 (Counterclockwise)

Preceding gene: 29140778

Following gene: 29140755

Centisome position: 5.8

GC content: 44.31

Gene sequence:

>255_bases
ATGAATGAAACGTATCAACCCATTAATTGCGATGACTACGATAGTCTTGAGCTTGCCTGCATGCATCATTTAATACTGAC
GCTAACGTTAAAAGATGGCGAGGTTTTGCAGGCAAAAGCGAACGATTTGATCTTACGTAAAAACGTAGAGTATCTGCTTG
CCGAGGTTTCCGGCGAGTCTTGTGAACTTCGTCTCGATAAAATCGCCAGTTTCAGCCACCCGGAAATTGGTACCGTGGTA
GTTAGCGAATCCTGA

Upstream 100 bases:

>100_bases
AAAGTGCTTAATGAAGTGGCGGCAGATAGCGCCCTTTCAGAGTCGGTCAGGGAAAAGGCAGCTTATGCTGCCGCGAACTT
ACTGGTGAGCGATTATGTCA

Downstream 100 bases:

>100_bases
GTATTTTGTTGCCGGATGGTGGTGCAAGCACCTTATCCGGCCTACGTTCCATGCGATACGTAGGCCTGATAAGCGCAGCG
CCATCAGGCATGCCAAACCA

Product: Rho-binding antiterminator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 84; Mature: 84

Protein sequence:

>84_residues
MNETYQPINCDDYDSLELACMHHLILTLTLKDGEVLQAKANDLILRKNVEYLLAEVSGESCELRLDKIASFSHPEIGTVV
VSES

Sequences:

>Translated_84_residues
MNETYQPINCDDYDSLELACMHHLILTLTLKDGEVLQAKANDLILRKNVEYLLAEVSGESCELRLDKIASFSHPEIGTVV
VSES
>Mature_84_residues
MNETYQPINCDDYDSLELACMHHLILTLTLKDGEVLQAKANDLILRKNVEYLLAEVSGESCELRLDKIASFSHPEIGTVV
VSES

Specific function: Suppresses temperature-sensitive mutations in essential genes by modulating rho-dependent transcription termination [H]

COG id: COG4568

COG function: function code K; Transcriptional antiterminator

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI87081698, Length=84, Percent_Identity=80.9523809523809, Blast_Score=144, Evalue=1e-36,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002730
- InterPro:   IPR009778 [H]

Pfam domain/function: PF07073 ROF [H]

EC number: NA

Molecular weight: Translated: 9395; Mature: 9395

Theoretical pI: Translated: 4.22; Mature: 4.22

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.6 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
6.0 %Cys+Met (Translated Protein)
3.6 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
6.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNETYQPINCDDYDSLELACMHHLILTLTLKDGEVLQAKANDLILRKNVEYLLAEVSGES
CCCCCCCCCCCCCCCHHHHHHHHEEEEEECCCCCEEEECCCCEEEECCHHEEEEECCCCC
CELRLDKIASFSHPEIGTVVVSES
EEEEEEHHCCCCCCCCCEEEEECC
>Mature Secondary Structure
MNETYQPINCDDYDSLELACMHHLILTLTLKDGEVLQAKANDLILRKNVEYLLAEVSGES
CCCCCCCCCCCCCCCHHHHHHHHEEEEEECCCCCEEEECCCCEEEECCHHEEEEECCCCC
CELRLDKIASFSHPEIGTVVVSES
EEEEEEHHCCCCCCCCCEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9723924; 9278503 [H]