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Identifier: 29140755

GI number: 29140755

Start: 249928

End: 250722

Strand: Reverse

Name: map

Synonym: t0216

Alternate gene names: 29140755

Gene position: 250722-249928 (Counterclockwise)

Preceding gene: 29140777

Following gene: 29140754

Centisome position: 5.23

GC content: 52.2

Gene sequence:

>795_bases
ATGGCTATCTCTATCAAGACATCTGAAGACATCGAAAAAATGCGCGTCGCGGGCCGTCTGGCAGCCGAAGTGCTGGAAAT
GATCGAACCGTATATCAAACCGGGCGTCACCACCGGCGAACTGGACCGTATCTGTAACGATTACATCGTTAACGAACAAC
ACGCGATCTCCGCCTGCCTCGGCTACCACGGCTATCCGAAATCCGTCTGTATCTCTATTAATGAAGTGGTGTGCCACGGC
ATTCCGGATGACGCTAAACATCTGAAAGATGGTGATATCGTCAACATCGACGTGACCGTGATTAAAGATGAATTCCACGG
CGATACCTCAAAGATGTTTATCGTCGGCAAACCGACTATTCTGGGCGAGCGTCTGTGCCGCGTCACGCAAGAGAGCCTGT
ATCTGGGCATCAAAATGGTGAAACCGGGCATCCGCCTGCGCACCATTGGCGCCGCCATCCAGAAATATGCCGAAGGCGAA
GGTTTCTCCGTGGTACGTGAATACTGCGGACACGGTATTGGTCGCGGCTTCCATGAAGAACCGCAGGTGCTGCATTATGA
TGCAGACGATGGTGGCGTCGTGTTGCAGCCTGGCATGACCTTTACCATTGAACCGATGCTGAATGCCGGGGATTACCGTA
TCCGCACCATGAAAGACGGCTGGACGGTAAAAACCAAAGACCGCAGCTTGTCTGCTCAGTACGAGCATACTATTGTGGTA
ACGGAAAACGGCTGTGAAATTCTGACGCTGCGTAAAGATGACACCATCCCGGCGATCATCACACACGACGAATAA

Upstream 100 bases:

>100_bases
CAGAATGATTCTCAATTTGGCAGGGTACACCCCTGACTGATACCATTGTCAGCACTTACACAATTATTGCCGAAATCATC
GGCACTGATGGACAGAATTC

Downstream 100 bases:

>100_bases
TGTTTTGCCTGATGGCGATGCCTTCGCATCTTATCAGGCCTACAAATTGTACTACTTCGTAGGCCGGATAAGGCGTTCAC
GCCACCATCCGGCTTTTTAA

Product: methionine aminopeptidase

Products: NA

Alternate protein names: MAP; Peptidase M

Number of amino acids: Translated: 264; Mature: 263

Protein sequence:

>264_residues
MAISIKTSEDIEKMRVAGRLAAEVLEMIEPYIKPGVTTGELDRICNDYIVNEQHAISACLGYHGYPKSVCISINEVVCHG
IPDDAKHLKDGDIVNIDVTVIKDEFHGDTSKMFIVGKPTILGERLCRVTQESLYLGIKMVKPGIRLRTIGAAIQKYAEGE
GFSVVREYCGHGIGRGFHEEPQVLHYDADDGGVVLQPGMTFTIEPMLNAGDYRIRTMKDGWTVKTKDRSLSAQYEHTIVV
TENGCEILTLRKDDTIPAIITHDE

Sequences:

>Translated_264_residues
MAISIKTSEDIEKMRVAGRLAAEVLEMIEPYIKPGVTTGELDRICNDYIVNEQHAISACLGYHGYPKSVCISINEVVCHG
IPDDAKHLKDGDIVNIDVTVIKDEFHGDTSKMFIVGKPTILGERLCRVTQESLYLGIKMVKPGIRLRTIGAAIQKYAEGE
GFSVVREYCGHGIGRGFHEEPQVLHYDADDGGVVLQPGMTFTIEPMLNAGDYRIRTMKDGWTVKTKDRSLSAQYEHTIVV
TENGCEILTLRKDDTIPAIITHDE
>Mature_263_residues
AISIKTSEDIEKMRVAGRLAAEVLEMIEPYIKPGVTTGELDRICNDYIVNEQHAISACLGYHGYPKSVCISINEVVCHGI
PDDAKHLKDGDIVNIDVTVIKDEFHGDTSKMFIVGKPTILGERLCRVTQESLYLGIKMVKPGIRLRTIGAAIQKYAEGEG
FSVVREYCGHGIGRGFHEEPQVLHYDADDGGVVLQPGMTFTIEPMLNAGDYRIRTMKDGWTVKTKDRSLSAQYEHTIVVT
ENGCEILTLRKDDTIPAIITHDE

Specific function: Removes the amino-terminal methionine from nascent proteins

COG id: COG0024

COG function: function code J; Methionine aminopeptidase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase M24A family

Homologues:

Organism=Homo sapiens, GI164420681, Length=260, Percent_Identity=46.1538461538462, Blast_Score=238, Evalue=4e-63,
Organism=Homo sapiens, GI40385867, Length=248, Percent_Identity=39.5161290322581, Blast_Score=189, Evalue=2e-48,
Organism=Escherichia coli, GI1786364, Length=264, Percent_Identity=89.3939393939394, Blast_Score=494, Evalue=1e-141,
Organism=Caenorhabditis elegans, GI71996291, Length=250, Percent_Identity=46.8, Blast_Score=224, Evalue=2e-59,
Organism=Saccharomyces cerevisiae, GI6323273, Length=251, Percent_Identity=44.6215139442231, Blast_Score=216, Evalue=4e-57,
Organism=Drosophila melanogaster, GI21355531, Length=253, Percent_Identity=47.0355731225296, Blast_Score=231, Evalue=3e-61,
Organism=Drosophila melanogaster, GI24583427, Length=245, Percent_Identity=44.0816326530612, Blast_Score=202, Evalue=1e-52,

Paralogues:

None

Copy number: 3,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): AMPM_SALTI (P0A1X7)

Other databases:

- EMBL:   AL627265
- EMBL:   AE014613
- RefSeq:   NP_454822.1
- RefSeq:   NP_804097.1
- ProteinModelPortal:   P0A1X7
- SMR:   P0A1X7
- MEROPS:   M24.001
- PRIDE:   P0A1X7
- GeneID:   1067809
- GeneID:   1246727
- GenomeReviews:   AE014613_GR
- GenomeReviews:   AL513382_GR
- KEGG:   stt:t0216
- KEGG:   sty:STY0238
- HOGENOM:   HBG299384
- OMA:   GIPDDGK
- ProtClustDB:   PRK05716
- BioCyc:   SENT209261:T0216-MONOMER
- BioCyc:   SENT220341:STY0238-MONOMER
- BRENDA:   3.4.11.18
- GO:   GO:0006508
- InterPro:   IPR001714
- InterPro:   IPR000994
- InterPro:   IPR002467
- Gene3D:   G3DSA:3.90.230.10
- PANTHER:   PTHR10804:SF13
- PANTHER:   PTHR10804
- PRINTS:   PR00599
- TIGRFAMs:   TIGR00500

Pfam domain/function: PF00557 Peptidase_M24; SSF55920 Peptidase_M24_cat_core

EC number: =3.4.11.18

Molecular weight: Translated: 29292; Mature: 29161

Theoretical pI: Translated: 5.37; Mature: 5.37

Prosite motif: PS00680 MAP_1

Important sites: BINDING 79-79 BINDING 178-178

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.7 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
5.7 %Cys+Met (Translated Protein)
2.7 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAISIKTSEDIEKMRVAGRLAAEVLEMIEPYIKPGVTTGELDRICNDYIVNEQHAISACL
CEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCHHHHHHHH
GYHGYPKSVCISINEVVCHGIPDDAKHLKDGDIVNIDVTVIKDEFHGDTSKMFIVGKPTI
CCCCCCCCEEEEEEHHEECCCCCHHHHCCCCCEEEEEEEEEEECCCCCCCEEEEEECCHH
LGERLCRVTQESLYLGIKMVKPGIRLRTIGAAIQKYAEGEGFSVVREYCGHGIGRGFHEE
HHHHHHHHHHHHHEEEEEEECCCCEEEHHHHHHHHHHCCCCHHHHHHHHCCCCCCCCCCC
PQVLHYDADDGGVVLQPGMTFTIEPMLNAGDYRIRTMKDGWTVKTKDRSLSAQYEHTIVV
CCEEEEECCCCCEEECCCCEEEEEECCCCCCEEEEEECCCCEEEECCCCCCCCEEEEEEE
TENGCEILTLRKDDTIPAIITHDE
ECCCCEEEEEECCCCCCEEEECCC
>Mature Secondary Structure 
AISIKTSEDIEKMRVAGRLAAEVLEMIEPYIKPGVTTGELDRICNDYIVNEQHAISACL
EEEECCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCHHHHHHHH
GYHGYPKSVCISINEVVCHGIPDDAKHLKDGDIVNIDVTVIKDEFHGDTSKMFIVGKPTI
CCCCCCCCEEEEEEHHEECCCCCHHHHCCCCCEEEEEEEEEEECCCCCCCEEEEEECCHH
LGERLCRVTQESLYLGIKMVKPGIRLRTIGAAIQKYAEGEGFSVVREYCGHGIGRGFHEE
HHHHHHHHHHHHHEEEEEEECCCCEEEHHHHHHHHHHCCCCHHHHHHHHCCCCCCCCCCC
PQVLHYDADDGGVVLQPGMTFTIEPMLNAGDYRIRTMKDGWTVKTKDRSLSAQYEHTIVV
CCEEEEECCCCCEEECCCCEEEEEECCCCCCEEEEEECCCCEEEECCCCCCCCEEEEEEE
TENGCEILTLRKDDTIPAIITHDE
ECCCCEEEEEECCCCCCEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11677608; 12644504