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The map label for this gene is folK [H]

Identifier: 29140732

GI number: 29140732

Start: 217797

End: 218276

Strand: Reverse

Name: folK [H]

Synonym: t0191

Alternate gene names: 29140732

Gene position: 218276-217797 (Counterclockwise)

Preceding gene: 29140733

Following gene: 29140731

Centisome position: 4.56

GC content: 57.92

Gene sequence:

>480_bases
ATGACGATCGCGTATATCGCGCTTGGCAGTAATCTGGCCTCTCCGCTGGAGCAGGTCAATGCCGCCCTGAAGGCTATCGC
CGACATTCCCGATAGCCGTATCGTCGCGGTTTCTTCGTTTTACCGCACGCCGCCGCTTGGCCCACAGGATCAGCCCGATT
ACCTGAACGCTGCGGTGGCGCTGGATACCGCTCTCGCCCCCGAAGAATTGCTTAACCATACCCAGCGCATTGAGCTGCAA
CAGGGCCGGGTGCGCAAGGCCGAACGCTGGGGGCCGCGCACGCTGGATCTCGACATTATGCTGTTTGGCGATGAAGTCAT
TAACACCGACCGTCTGACCGTGCCGCACTACGACATGAAAAACCGCGGTTTTATGCTGTGGCCGTTATTCGAAATCGCTC
CGGAGCTGGTGTTTCCCGACGATAAAGGTCTGCTGATCGAGCTGATGTCCCAAATGTCTCCCTGTCCCGATCGTTGGTAA

Upstream 100 bases:

>100_bases
CGGAACAAAAAGGGATGCTCAACGAGCTGGACGACGATCCTGCTCCACGCCGCCGTCGTTCACGTCCGCGCAAACGCGCG
CCGCGCCGCGAGGGCACCGT

Downstream 100 bases:

>100_bases
GAAATACCCCTATTATCAATTCAACAAATAGTTTCTATGCTGTTATTCCGTATAATTAGATAAATTAAATACTCTTGTTA
GAAATAATCATGGAACCAGT

Product: 2-amino-4-hydroxy-6-hydroxymethyldihyropteridine pyrophosphokinase

Products: NA

Alternate protein names: 6-hydroxymethyl-7,8-dihydropterin pyrophosphokinase; PPPK; 7,8-dihydro-6-hydroxymethylpterin-pyrophosphokinase; HPPK [H]

Number of amino acids: Translated: 159; Mature: 158

Protein sequence:

>159_residues
MTIAYIALGSNLASPLEQVNAALKAIADIPDSRIVAVSSFYRTPPLGPQDQPDYLNAAVALDTALAPEELLNHTQRIELQ
QGRVRKAERWGPRTLDLDIMLFGDEVINTDRLTVPHYDMKNRGFMLWPLFEIAPELVFPDDKGLLIELMSQMSPCPDRW

Sequences:

>Translated_159_residues
MTIAYIALGSNLASPLEQVNAALKAIADIPDSRIVAVSSFYRTPPLGPQDQPDYLNAAVALDTALAPEELLNHTQRIELQ
QGRVRKAERWGPRTLDLDIMLFGDEVINTDRLTVPHYDMKNRGFMLWPLFEIAPELVFPDDKGLLIELMSQMSPCPDRW
>Mature_158_residues
TIAYIALGSNLASPLEQVNAALKAIADIPDSRIVAVSSFYRTPPLGPQDQPDYLNAAVALDTALAPEELLNHTQRIELQQ
GRVRKAERWGPRTLDLDIMLFGDEVINTDRLTVPHYDMKNRGFMLWPLFEIAPELVFPDDKGLLIELMSQMSPCPDRW

Specific function: Dihydrofolate biosynthesis; first step. [C]

COG id: COG0801

COG function: function code H; 7,8-dihydro-6-hydroxymethylpterin-pyrophosphokinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the HPPK family [H]

Homologues:

Organism=Escherichia coli, GI1786335, Length=159, Percent_Identity=82.3899371069182, Blast_Score=271, Evalue=1e-74,
Organism=Saccharomyces cerevisiae, GI6324073, Length=155, Percent_Identity=30.9677419354839, Blast_Score=83, Evalue=2e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000550 [H]

Pfam domain/function: PF01288 HPPK [H]

EC number: =2.7.6.3 [H]

Molecular weight: Translated: 17888; Mature: 17757

Theoretical pI: Translated: 4.42; Mature: 4.42

Prosite motif: PS00794 HPPK

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTIAYIALGSNLASPLEQVNAALKAIADIPDSRIVAVSSFYRTPPLGPQDQPDYLNAAVA
CEEEEEEECCHHHHHHHHHHHHHHHHHCCCCCCEEEEEHHHCCCCCCCCCCCCHHHHHHH
LDTALAPEELLNHTQRIELQQGRVRKAERWGPRTLDLDIMLFGDEVINTDRLTVPHYDMK
HHHCCCHHHHHHHHHHHHHHHCCHHHHHHCCCEEEEEEEEEECCCCCCCCCEECCCCCCC
NRGFMLWPLFEIAPELVFPDDKGLLIELMSQMSPCPDRW
CCCEEEEEHHHHCCHHCCCCCCCHHHHHHHHCCCCCCCC
>Mature Secondary Structure 
TIAYIALGSNLASPLEQVNAALKAIADIPDSRIVAVSSFYRTPPLGPQDQPDYLNAAVA
EEEEEEECCHHHHHHHHHHHHHHHHHCCCCCCEEEEEHHHCCCCCCCCCCCCHHHHHHH
LDTALAPEELLNHTQRIELQQGRVRKAERWGPRTLDLDIMLFGDEVINTDRLTVPHYDMK
HHHCCCHHHHHHHHHHHHHHHCCHHHHHHCCCEEEEEEEEEECCCCCCCCCEECCCCCCC
NRGFMLWPLFEIAPELVFPDDKGLLIELMSQMSPCPDRW
CCCEEEEEHHHHCCHHCCCCCCCHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1325970; 8202364; 9278503; 1657875; 2537812; 10378268; 10452528 [H]