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The map label for this gene is staB [H]

Identifier: 29140730

GI number: 29140730

Start: 215905

End: 216639

Strand: Reverse

Name: staB [H]

Synonym: t0189

Alternate gene names: 29140730

Gene position: 216639-215905 (Counterclockwise)

Preceding gene: 29140731

Following gene: 29140729

Centisome position: 4.52

GC content: 46.8

Gene sequence:

>735_bases
ATGTTGTATGTAAAAAAAAGCCTGACCGCCATCGTATTAGCCAGTGCTGTATTTAGTACCTTTAGCTATGCGGATATTAT
TATCTCGGGCACCAGAATCGTTTATGACGAAAATAAAAAAGACGTCAGTATACGACTGGAAAATAAGGGTACCCGCCCAT
TATTAGTCCAAAACTGGATAGACCTGGGTAACGATAACGACGATCCCGGGAGTATTAAAGCGCCTTTCGTTTCTACCCCC
CCGGTTTCACGCGTTGAGCCTAAACGTGGGCAATCGGTAAAAATTATGTATACCCAGGCCAGCGCGCTGCCGAAAGACCG
GGAAAGCGTCTTCTGGTTTAACGTACTGGAAGTACCGCCAAAAGCGGATACCAGCAAAGAAGAGAATAAAAGCTTACTGC
AACTGGCTTTTCGTACCCGTATCAAACTGTTCTATCGCCCAACAGGACTAAAAGGCCAGCCTGCTGAAGCGGCAAAACAA
CTGAAGTGGCAAATAGCGAGCGAACAAGGCTACGCGGTACTACGTGCTGATAACTCAACTCCGTATTTCGTCTCATTTAA
TGACGCTACCTACACTGCAGGCGGTAAACGTTATGACGTTGAAGCCACCATGGTTGCGCCTTTCAGTAAATCATCCTTCA
CCGTGAAAGGCTTGTCAGGCGCTAGCGCCGGAAAACTGGAGTATCACGCCATTAATGATTTTGGCGGTTTGATTGAAGGT
TCCGTATCGCTGTAA

Upstream 100 bases:

>100_bases
ACCTTCGGGGTCCAGGAATTATTTCAACGATTCCGGGGGTTCATTCAATTTAATTAAATTCAAAGTTGATTTCATTATCT
TCTACAGGCAAGGAACGCTT

Downstream 100 bases:

>100_bases
CGGATTAACCAAGAATCAGGATGAAGAAATCGGTGTAAAACTTTCCACATTCCAAGGCCATGCCGAAGAAAACGATGCTC
GCAGTTTGCTGTGCGCTATT

Product: chaperone protein EcpD

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 244; Mature: 244

Protein sequence:

>244_residues
MLYVKKSLTAIVLASAVFSTFSYADIIISGTRIVYDENKKDVSIRLENKGTRPLLVQNWIDLGNDNDDPGSIKAPFVSTP
PVSRVEPKRGQSVKIMYTQASALPKDRESVFWFNVLEVPPKADTSKEENKSLLQLAFRTRIKLFYRPTGLKGQPAEAAKQ
LKWQIASEQGYAVLRADNSTPYFVSFNDATYTAGGKRYDVEATMVAPFSKSSFTVKGLSGASAGKLEYHAINDFGGLIEG
SVSL

Sequences:

>Translated_244_residues
MLYVKKSLTAIVLASAVFSTFSYADIIISGTRIVYDENKKDVSIRLENKGTRPLLVQNWIDLGNDNDDPGSIKAPFVSTP
PVSRVEPKRGQSVKIMYTQASALPKDRESVFWFNVLEVPPKADTSKEENKSLLQLAFRTRIKLFYRPTGLKGQPAEAAKQ
LKWQIASEQGYAVLRADNSTPYFVSFNDATYTAGGKRYDVEATMVAPFSKSSFTVKGLSGASAGKLEYHAINDFGGLIEG
SVSL
>Mature_244_residues
MLYVKKSLTAIVLASAVFSTFSYADIIISGTRIVYDENKKDVSIRLENKGTRPLLVQNWIDLGNDNDDPGSIKAPFVSTP
PVSRVEPKRGQSVKIMYTQASALPKDRESVFWFNVLEVPPKADTSKEENKSLLQLAFRTRIKLFYRPTGLKGQPAEAAKQ
LKWQIASEQGYAVLRADNSTPYFVSFNDATYTAGGKRYDVEATMVAPFSKSSFTVKGLSGASAGKLEYHAINDFGGLIEG
SVSL

Specific function: Probably mediates assembly of a pilin [H]

COG id: COG3121

COG function: function code NU; P pilus assembly protein, chaperone PapD

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Ig-like (immunoglobulin-like) domain [H]

Homologues:

Organism=Escherichia coli, GI1786333, Length=246, Percent_Identity=57.3170731707317, Blast_Score=299, Evalue=1e-82,
Organism=Escherichia coli, GI1788428, Length=237, Percent_Identity=33.7552742616034, Blast_Score=130, Evalue=8e-32,
Organism=Escherichia coli, GI1789532, Length=213, Percent_Identity=35.6807511737089, Blast_Score=112, Evalue=2e-26,
Organism=Escherichia coli, GI1786936, Length=211, Percent_Identity=35.5450236966825, Blast_Score=108, Evalue=3e-25,
Organism=Escherichia coli, GI1787171, Length=234, Percent_Identity=32.9059829059829, Blast_Score=108, Evalue=4e-25,
Organism=Escherichia coli, GI1786743, Length=239, Percent_Identity=30.1255230125523, Blast_Score=100, Evalue=9e-23,
Organism=Escherichia coli, GI87082203, Length=239, Percent_Identity=30.1255230125523, Blast_Score=100, Evalue=1e-22,
Organism=Escherichia coli, GI1790771, Length=215, Percent_Identity=30.6976744186047, Blast_Score=99, Evalue=2e-22,
Organism=Escherichia coli, GI1788677, Length=211, Percent_Identity=33.175355450237, Blast_Score=97, Evalue=1e-21,
Organism=Escherichia coli, GI87081806, Length=234, Percent_Identity=29.0598290598291, Blast_Score=86, Evalue=3e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008962
- InterPro:   IPR001829
- InterPro:   IPR016148
- InterPro:   IPR018046
- InterPro:   IPR016147 [H]

Pfam domain/function: PF02753 Pili_assembly_C; PF00345 Pili_assembly_N [H]

EC number: NA

Molecular weight: Translated: 26861; Mature: 26861

Theoretical pI: Translated: 9.79; Mature: 9.79

Prosite motif: PS00635 PILI_CHAPERONE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLYVKKSLTAIVLASAVFSTFSYADIIISGTRIVYDENKKDVSIRLENKGTRPLLVQNWI
CEEEHHHHHHHHHHHHHHHHCCEEEEEEECEEEEEECCCCEEEEEECCCCCCCEEEEEHH
DLGNDNDDPGSIKAPFVSTPPVSRVEPKRGQSVKIMYTQASALPKDRESVFWFNVLEVPP
HCCCCCCCCCCEECCEECCCCCCCCCCCCCCEEEEEEEECCCCCCCCCEEEEEEEEECCC
KADTSKEENKSLLQLAFRTRIKLFYRPTGLKGQPAEAAKQLKWQIASEQGYAVLRADNST
CCCCCCHHHHHHHHHHHHHEEEEEECCCCCCCCCHHHHHHHEEEEECCCCEEEEEECCCC
PYFVSFNDATYTAGGKRYDVEATMVAPFSKSSFTVKGLSGASAGKLEYHAINDFGGLIEG
CEEEEECCEEEECCCEEEEEEEEEEECCCCCCEEEEECCCCCCCEEEEEEECCCCCEEEC
SVSL
CCCC
>Mature Secondary Structure
MLYVKKSLTAIVLASAVFSTFSYADIIISGTRIVYDENKKDVSIRLENKGTRPLLVQNWI
CEEEHHHHHHHHHHHHHHHHCCEEEEEEECEEEEEECCCCEEEEEECCCCCCCEEEEEHH
DLGNDNDDPGSIKAPFVSTPPVSRVEPKRGQSVKIMYTQASALPKDRESVFWFNVLEVPP
HCCCCCCCCCCEECCEECCCCCCCCCCCCCCEEEEEEEECCCCCCCCCEEEEEEEEECCC
KADTSKEENKSLLQLAFRTRIKLFYRPTGLKGQPAEAAKQLKWQIASEQGYAVLRADNST
CCCCCCHHHHHHHHHHHHHEEEEEECCCCCCCCCHHHHHHHEEEEECCCCEEEEEECCCC
PYFVSFNDATYTAGGKRYDVEATMVAPFSKSSFTVKGLSGASAGKLEYHAINDFGGLIEG
CEEEEECCEEEECCCEEEEEEEEEEECCCCCCEEEEECCCCCCCEEEEEEECCCCCEEEC
SVSL
CCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8102362; 8202364; 9278503 [H]