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The map label for this gene is yacK

Identifier: 29140714

GI number: 29140714

Start: 198711

End: 200321

Strand: Direct

Name: yacK

Synonym: t0173

Alternate gene names: 29140714

Gene position: 198711-200321 (Clockwise)

Preceding gene: 29140710

Following gene: 29140716

Centisome position: 4.15

GC content: 56.24

Gene sequence:

>1611_bases
ATGTTACGCCGTGATTTCTTAAAATATTCAGTGGCGCTGGGGGTTGCATCAGCGCTGCCGCTGTGGAGCCGCGCCGCTTT
TGCCGCCGAACGTCCCGCGTTGCCTATTCCTGACCTGTTAACGGCAGATGCGAGCAACCGTATGCAGTTAATTGTTAAAG
CCGGACAGTCGACATTCGCCGGTAAGAACGCGACAACCTGGGGCTACAACGGTAATTTGCTGGGGCCAGCGGTACAGCTT
CACAAAGGAAAAAGCGTGACCGTTGATATCCATAACCAACTGGCCGAAGACACGACGCTTCACTGGCATGGTCTGGAGAT
TCCGGGCATCGTTGACGGCGGCCCGCAGGGGATTATTCCCGCTGGCGGAACCCGCACGGTGACGTTTACGCCGCAGCAAC
GCGCCGCGACCTGCTGGATTCATCCGCACAAACACGGCAAAACCGGGCGCCAGGTGGCGATGGGCCTCGCCGGACTGGTG
CTGATTGAAGATGACGAGATTCGCAAATTGCGCCTGCCGAAACAGTGGGGTATCGACGATGTGCCGGTGATCATTCAGGA
CAAACGCTTCTCCGCCGATGGCCAGATTGATTATCAACTGGATATTATGACCGCCGCCGTCGGCTGGTTTGGCGATACGC
TGCTGACCAATGGCGCTATCTATCCGCAGCATTCCGCGCCGAAAGGCTGGCTACGTCTGCGCTTGCTGAATGGCTGTAAT
GCGCGTTCGCTGAATATCGCCGCCAGCGATAATCGTCCGCTTTATGTGATCGCCAGCGACGGCGGCCTGCTGGCGGAGCC
GGTGAAAGTCACCGAACTGCCGTTATTAATGGGCGAGCGTTTTGAAGTGCTGGTGGATATCAGCGACGGGAAAGCCTTTG
ATCTGGTGACCCTGCCGGTCAGCCAGATGGGAATGGCGATCGCTCCGTTTGATAAACCGCATCCGGTGATGCGTATCCAG
CCGCTGCGGATTACCGCCTCCGGTACGCTGCCGGATACGTTGACGACGATGCCGGCGCTGCCGTCGCTGGAAGGGCTGAC
GGTGCGCAACCTGAAACTGTCGATGGACCCGCGCCTTGATATGATGGGGATGCAAATGCTGATGAAGAAATATGGCGCTC
AGGCGATGAGCGGCATGGATCATGACAGCATGAACGCGCATATGCAGGGCGGCAATATGGGGCATGGCGAGATGGATCAT
GGCAACATGGATCACAGCGGGATGAATCATGGCGCGATGGGCAATATGAATCACGGCGGAAAATTCGACTTCCATAACGC
TAACTTTATCAACGGCCAGGTCTTCGATATGAACAAACCGATGTTCGCGGCGCAAAAAGGCCGACATGAACGTTGGGTGA
TTTCCGGCGTGGGTGACATGATGCTGCATCCTTTCCATATTCACGGCACCCAGTTCCGTATTTTGTCAGAGAACGGCAAA
GCGCCAGCGGCGCACAGAACGGGCTGGAAGGATACGGTACGCGTTGAGGGCGGTATCAGCGAAGTGCTGGTCAAGTTCGA
TCACGACGCGCCGAAGGAACATGCCTATATGGCGCACTGTCATCTGTTAGAACATGAAGATACGGGAATGATGTTAGGAT
TTACGGTCTAA

Upstream 100 bases:

>100_bases
ATTGCTGTCAGGAACGGTAAAGACGTGTTGCTGGCTTGACCTTCCCGTTAGGGCAGGGTCTAAGCTTAGACACCCGCCTG
TTCATTATAAGGAAATGATT

Downstream 100 bases:

>100_bases
TCGATGTCTGTGACGCAGGCCGGGTAAGGCGAAACCGCTACCCGGCACAATGCCCGGCATACGCCGGGCATTAATGGTTA
TTTCGCGTCGTCAGGCAAAG

Product: multicopper oxidase

Products: NA

Alternate protein names: Copper efflux oxidase

Number of amino acids: Translated: 536; Mature: 536

Protein sequence:

>536_residues
MLRRDFLKYSVALGVASALPLWSRAAFAAERPALPIPDLLTADASNRMQLIVKAGQSTFAGKNATTWGYNGNLLGPAVQL
HKGKSVTVDIHNQLAEDTTLHWHGLEIPGIVDGGPQGIIPAGGTRTVTFTPQQRAATCWIHPHKHGKTGRQVAMGLAGLV
LIEDDEIRKLRLPKQWGIDDVPVIIQDKRFSADGQIDYQLDIMTAAVGWFGDTLLTNGAIYPQHSAPKGWLRLRLLNGCN
ARSLNIAASDNRPLYVIASDGGLLAEPVKVTELPLLMGERFEVLVDISDGKAFDLVTLPVSQMGMAIAPFDKPHPVMRIQ
PLRITASGTLPDTLTTMPALPSLEGLTVRNLKLSMDPRLDMMGMQMLMKKYGAQAMSGMDHDSMNAHMQGGNMGHGEMDH
GNMDHSGMNHGAMGNMNHGGKFDFHNANFINGQVFDMNKPMFAAQKGRHERWVISGVGDMMLHPFHIHGTQFRILSENGK
APAAHRTGWKDTVRVEGGISEVLVKFDHDAPKEHAYMAHCHLLEHEDTGMMLGFTV

Sequences:

>Translated_536_residues
MLRRDFLKYSVALGVASALPLWSRAAFAAERPALPIPDLLTADASNRMQLIVKAGQSTFAGKNATTWGYNGNLLGPAVQL
HKGKSVTVDIHNQLAEDTTLHWHGLEIPGIVDGGPQGIIPAGGTRTVTFTPQQRAATCWIHPHKHGKTGRQVAMGLAGLV
LIEDDEIRKLRLPKQWGIDDVPVIIQDKRFSADGQIDYQLDIMTAAVGWFGDTLLTNGAIYPQHSAPKGWLRLRLLNGCN
ARSLNIAASDNRPLYVIASDGGLLAEPVKVTELPLLMGERFEVLVDISDGKAFDLVTLPVSQMGMAIAPFDKPHPVMRIQ
PLRITASGTLPDTLTTMPALPSLEGLTVRNLKLSMDPRLDMMGMQMLMKKYGAQAMSGMDHDSMNAHMQGGNMGHGEMDH
GNMDHSGMNHGAMGNMNHGGKFDFHNANFINGQVFDMNKPMFAAQKGRHERWVISGVGDMMLHPFHIHGTQFRILSENGK
APAAHRTGWKDTVRVEGGISEVLVKFDHDAPKEHAYMAHCHLLEHEDTGMMLGFTV
>Mature_536_residues
MLRRDFLKYSVALGVASALPLWSRAAFAAERPALPIPDLLTADASNRMQLIVKAGQSTFAGKNATTWGYNGNLLGPAVQL
HKGKSVTVDIHNQLAEDTTLHWHGLEIPGIVDGGPQGIIPAGGTRTVTFTPQQRAATCWIHPHKHGKTGRQVAMGLAGLV
LIEDDEIRKLRLPKQWGIDDVPVIIQDKRFSADGQIDYQLDIMTAAVGWFGDTLLTNGAIYPQHSAPKGWLRLRLLNGCN
ARSLNIAASDNRPLYVIASDGGLLAEPVKVTELPLLMGERFEVLVDISDGKAFDLVTLPVSQMGMAIAPFDKPHPVMRIQ
PLRITASGTLPDTLTTMPALPSLEGLTVRNLKLSMDPRLDMMGMQMLMKKYGAQAMSGMDHDSMNAHMQGGNMGHGEMDH
GNMDHSGMNHGAMGNMNHGGKFDFHNANFINGQVFDMNKPMFAAQKGRHERWVISGVGDMMLHPFHIHGTQFRILSENGK
APAAHRTGWKDTVRVEGGISEVLVKFDHDAPKEHAYMAHCHLLEHEDTGMMLGFTV

Specific function: Probably involved in periplasmic detoxification of copper by oxidizing Cu(+) to Cu(2+) and thus preventing its uptake into the cytoplasm. Possesses phenoloxidase and ferroxidase activities and might be involved in the production of polyphenolic compounds

COG id: COG2132

COG function: function code Q; Putative multicopper oxidases

Gene ontology:

Cell location: Periplasm

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 3 plastocyanin-like domains

Homologues:

Organism=Escherichia coli, GI1786314, Length=536, Percent_Identity=81.9029850746269, Blast_Score=907, Evalue=0.0,
Organism=Escherichia coli, GI1789394, Length=391, Percent_Identity=33.5038363171356, Blast_Score=205, Evalue=6e-54,

Paralogues:

None

Copy number: 40 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): CUEO_SALTI (Q8Z9E1)

Other databases:

- EMBL:   AL627265
- EMBL:   AE014613
- RefSeq:   NP_454781.1
- RefSeq:   NP_804056.1
- ProteinModelPortal:   Q8Z9E1
- SMR:   Q8Z9E1
- GeneID:   1067759
- GeneID:   1246684
- GenomeReviews:   AE014613_GR
- GenomeReviews:   AL513382_GR
- KEGG:   stt:t0173
- KEGG:   sty:STY0190
- HOGENOM:   HBG655385
- OMA:   HGMKLPA
- ProtClustDB:   PRK10965
- BioCyc:   SENT209261:T0173-MONOMER
- BioCyc:   SENT220341:STY0190-MONOMER
- InterPro:   IPR001117
- InterPro:   IPR011706
- InterPro:   IPR011707
- InterPro:   IPR002355
- InterPro:   IPR008972
- InterPro:   IPR006311
- Gene3D:   G3DSA:2.60.40.420
- TIGRFAMs:   TIGR01409

Pfam domain/function: PF00394 Cu-oxidase; PF07731 Cu-oxidase_2; PF07732 Cu-oxidase_3; SSF49503 Cupredoxin

EC number: NA

Molecular weight: Translated: 58625; Mature: 58625

Theoretical pI: Translated: 7.24; Mature: 7.24

Prosite motif: PS00080 MULTICOPPER_OXIDASE2; PS51318 TAT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
6.0 %Met     (Translated Protein)
6.5 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
6.0 %Met     (Mature Protein)
6.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLRRDFLKYSVALGVASALPLWSRAAFAAERPALPIPDLLTADASNRMQLIVKAGQSTFA
CCCHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCCCHHEECCCCCCEEEEEECCCCCCC
GKNATTWGYNGNLLGPAVQLHKGKSVTVDIHNQLAEDTTLHWHGLEIPGIVDGGPQGIIP
CCCCEECCCCCCCCCCEEEEECCCEEEEEEHHHCCCCCEEEEECCCCCCEECCCCCCEEC
AGGTRTVTFTPQQRAATCWIHPHKHGKTGRQVAMGLAGLVLIEDDEIRKLRLPKQWGIDD
CCCCEEEEECCCCCCEEEEEECCCCCCCHHHHHHHHEEEEEEECCCCEEEECCHHCCCCC
VPVIIQDKRFSADGQIDYQLDIMTAAVGWFGDTLLTNGAIYPQHSAPKGWLRLRLLNGCN
CCEEEECCCCCCCCEEEEEEEEEEHHHHHHCHHHHCCCEECCCCCCCCCEEEEEEECCCC
ARSLNIAASDNRPLYVIASDGGLLAEPVKVTELPLLMGERFEVLVDISDGKAFDLVTLPV
CCEEEEEECCCCCEEEEECCCCEEECCCEEEECCCCCCCCEEEEEEECCCCEEEEEEECH
SQMGMAIAPFDKPHPVMRIQPLRITASGTLPDTLTTMPALPSLEGLTVRNLKLSMDPRLD
HHCCEEEECCCCCCCCEEEEEEEEEECCCCCCHHHHCCCCCCCCCEEEEEEEEECCCCHH
MMGMQMLMKKYGAQAMSGMDHDSMNAHMQGGNMGHGEMDHGNMDHSGMNHGAMGNMNHGG
HHHHHHHHHHHCHHHHCCCCCCCCEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
KFDFHNANFINGQVFDMNKPMFAAQKGRHERWVISGVGDMMLHPFHIHGTQFRILSENGK
EEECCCCCEECCEEEECCCCCHHHHCCCCCEEEEECCCCHHCCEEEECCEEEEEEECCCC
APAAHRTGWKDTVRVEGGISEVLVKFDHDAPKEHAYMAHCHLLEHEDTGMMLGFTV
CCCCCCCCCCCEEEECCCHHHEEEEECCCCCCCCEEEEEEEEEEECCCCEEEEEEC
>Mature Secondary Structure
MLRRDFLKYSVALGVASALPLWSRAAFAAERPALPIPDLLTADASNRMQLIVKAGQSTFA
CCCHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCCCHHEECCCCCCEEEEEECCCCCCC
GKNATTWGYNGNLLGPAVQLHKGKSVTVDIHNQLAEDTTLHWHGLEIPGIVDGGPQGIIP
CCCCEECCCCCCCCCCEEEEECCCEEEEEEHHHCCCCCEEEEECCCCCCEECCCCCCEEC
AGGTRTVTFTPQQRAATCWIHPHKHGKTGRQVAMGLAGLVLIEDDEIRKLRLPKQWGIDD
CCCCEEEEECCCCCCEEEEEECCCCCCCHHHHHHHHEEEEEEECCCCEEEECCHHCCCCC
VPVIIQDKRFSADGQIDYQLDIMTAAVGWFGDTLLTNGAIYPQHSAPKGWLRLRLLNGCN
CCEEEECCCCCCCCEEEEEEEEEEHHHHHHCHHHHCCCEECCCCCCCCCEEEEEEECCCC
ARSLNIAASDNRPLYVIASDGGLLAEPVKVTELPLLMGERFEVLVDISDGKAFDLVTLPV
CCEEEEEECCCCCEEEEECCCCEEECCCEEEECCCCCCCCEEEEEEECCCCEEEEEEECH
SQMGMAIAPFDKPHPVMRIQPLRITASGTLPDTLTTMPALPSLEGLTVRNLKLSMDPRLD
HHCCEEEECCCCCCCCEEEEEEEEEECCCCCCHHHHCCCCCCCCCEEEEEEEEECCCCHH
MMGMQMLMKKYGAQAMSGMDHDSMNAHMQGGNMGHGEMDHGNMDHSGMNHGAMGNMNHGG
HHHHHHHHHHHCHHHHCCCCCCCCEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
KFDFHNANFINGQVFDMNKPMFAAQKGRHERWVISGVGDMMLHPFHIHGTQFRILSENGK
EEECCCCCEECCEEEECCCCCHHHHCCCCCEEEEECCCCHHCCEEEECCEEEEEEECCCC
APAAHRTGWKDTVRVEGGISEVLVKFDHDAPKEHAYMAHCHLLEHEDTGMMLGFTV
CCCCCCCCCCCEEEECCCHHHEEEEECCCCCCCCEEEEEEEEEEECCCCEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11677608; 12644504