Click here to switch to the map view.

The map label for this gene is hcaR [H]

Identifier: 28378585

GI number: 28378585

Start: 1756858

End: 1757748

Strand: Direct

Name: hcaR [H]

Synonym: lp_1938

Alternate gene names: 28378585

Gene position: 1756858-1757748 (Clockwise)

Preceding gene: 28378574

Following gene: 28378586

Centisome position: 53.1

GC content: 46.58

Gene sequence:

>891_bases
ATGGAATTACGAGTTCTACGTTATTTTCAAGCAGTGGTCGCCGAGTTAAATATCAGTCGGGCCGCTGAACGCTTACACGT
CTCGCAGCCAACAATTTCACGACAATTAAAGGATTTAGAGGATGAACTGGGCGCGACCCTATTTGAACGCAATGGCCGTC
ATATCAGTCTCACGAGTAGTGGCGAATACTTTGCCACGCAGGCGAACCAAATCATCGCCTTAGCCGATAAGACGCTTGCC
AACATTAATACAGCCAAAGAAATCAGTGGCACGATTGAATTAGGGAGTGCCGAGATGCGCAGTTTTTTGACGATTGCTCA
GTCGATTAAGAAACTACAACAACAGTATCCGAAAATCAGGATCAACGTGACGAGTAGTAATGCGAATCAGATTCGAACCA
ACCTCAAAACCGGGAACTTTGACTTTGGAATCGTCACGGAACCAACTGACAAACACGACTTAAACTTTATCCATCTACCC
GGCGAAAGTCGCTGGGGATTACTGGTTCCGCGGCATTCGCCGCTAGCTGACCATGATTACATTAGCTTAGCTGATCTTGA
AGGCCAAAAGATCATTGTTTCTGCACAACACGGTACGATCAATCAACTTCAGGATTGGTATGGTGAAAGCACGCCAAAAT
TCACCATCGTCGCCACTTATAATTTACTCTATAATGCCTCGTTACTAGTTTCTGCGGGCGTCGGGTACGCGTTGGGGATT
GATGGCATCATCAATACGAACCAATCCGATTTGATTTTCATCCCACTAACACCTCGAATAACCGCCCGCACTAGCCTAGT
CTGGACTAAGGGACAACGCTTGTCGGAAGCCGCCAAAACCTTTTTAACGCAATTGGCGACAGACCTGCAGCAACAAATAC
CTACTGACTGA

Upstream 100 bases:

>100_bases
ACTAACCTCAAGTTTCATCTTAAAGCTTGACAGCAACCATGTCGAATGCTTATATGGTATTTAAAATTATACTTAATTGT
AATAGAAAGAAGTGCTTTAG

Downstream 100 bases:

>100_bases
CACTATCGACTCAGCTTTCACCAATCACCACAACACACTGCCCAATTGATGGCTGCTGAAGACGACGCGCATCTAATTCA
CGCTAGTCCGAACTAAATAA

Product: transcription regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 296; Mature: 296

Protein sequence:

>296_residues
MELRVLRYFQAVVAELNISRAAERLHVSQPTISRQLKDLEDELGATLFERNGRHISLTSSGEYFATQANQIIALADKTLA
NINTAKEISGTIELGSAEMRSFLTIAQSIKKLQQQYPKIRINVTSSNANQIRTNLKTGNFDFGIVTEPTDKHDLNFIHLP
GESRWGLLVPRHSPLADHDYISLADLEGQKIIVSAQHGTINQLQDWYGESTPKFTIVATYNLLYNASLLVSAGVGYALGI
DGIINTNQSDLIFIPLTPRITARTSLVWTKGQRLSEAAKTFLTQLATDLQQQIPTD

Sequences:

>Translated_296_residues
MELRVLRYFQAVVAELNISRAAERLHVSQPTISRQLKDLEDELGATLFERNGRHISLTSSGEYFATQANQIIALADKTLA
NINTAKEISGTIELGSAEMRSFLTIAQSIKKLQQQYPKIRINVTSSNANQIRTNLKTGNFDFGIVTEPTDKHDLNFIHLP
GESRWGLLVPRHSPLADHDYISLADLEGQKIIVSAQHGTINQLQDWYGESTPKFTIVATYNLLYNASLLVSAGVGYALGI
DGIINTNQSDLIFIPLTPRITARTSLVWTKGQRLSEAAKTFLTQLATDLQQQIPTD
>Mature_296_residues
MELRVLRYFQAVVAELNISRAAERLHVSQPTISRQLKDLEDELGATLFERNGRHISLTSSGEYFATQANQIIALADKTLA
NINTAKEISGTIELGSAEMRSFLTIAQSIKKLQQQYPKIRINVTSSNANQIRTNLKTGNFDFGIVTEPTDKHDLNFIHLP
GESRWGLLVPRHSPLADHDYISLADLEGQKIIVSAQHGTINQLQDWYGESTPKFTIVATYNLLYNASLLVSAGVGYALGI
DGIINTNQSDLIFIPLTPRITARTSLVWTKGQRLSEAAKTFLTQLATDLQQQIPTD

Specific function: Transcriptional activator of the hca operon for 3- phenylpropionic acid catabolism [H]

COG id: COG0583

COG function: function code K; Transcriptional regulator

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1788887, Length=289, Percent_Identity=28.719723183391, Blast_Score=109, Evalue=3e-25,
Organism=Escherichia coli, GI1787879, Length=299, Percent_Identity=26.4214046822742, Blast_Score=104, Evalue=7e-24,
Organism=Escherichia coli, GI157672245, Length=287, Percent_Identity=30.3135888501742, Blast_Score=103, Evalue=1e-23,
Organism=Escherichia coli, GI1788748, Length=292, Percent_Identity=27.3972602739726, Blast_Score=98, Evalue=5e-22,
Organism=Escherichia coli, GI145693105, Length=302, Percent_Identity=26.8211920529801, Blast_Score=93, Evalue=2e-20,
Organism=Escherichia coli, GI1787530, Length=265, Percent_Identity=27.1698113207547, Blast_Score=90, Evalue=1e-19,
Organism=Escherichia coli, GI1790399, Length=196, Percent_Identity=29.5918367346939, Blast_Score=88, Evalue=7e-19,
Organism=Escherichia coli, GI2367136, Length=276, Percent_Identity=26.4492753623188, Blast_Score=87, Evalue=9e-19,
Organism=Escherichia coli, GI1788296, Length=200, Percent_Identity=27.5, Blast_Score=75, Evalue=7e-15,
Organism=Escherichia coli, GI87081904, Length=208, Percent_Identity=27.8846153846154, Blast_Score=70, Evalue=1e-13,
Organism=Escherichia coli, GI1787806, Length=157, Percent_Identity=26.1146496815287, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1790208, Length=149, Percent_Identity=26.8456375838926, Blast_Score=67, Evalue=9e-13,
Organism=Escherichia coli, GI1787601, Length=152, Percent_Identity=26.9736842105263, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI87082132, Length=181, Percent_Identity=27.0718232044199, Blast_Score=65, Evalue=5e-12,
Organism=Escherichia coli, GI1788297, Length=149, Percent_Identity=27.5167785234899, Blast_Score=65, Evalue=7e-12,
Organism=Escherichia coli, GI1789204, Length=246, Percent_Identity=23.5772357723577, Blast_Score=62, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 32862; Mature: 32862

Theoretical pI: Translated: 6.90; Mature: 6.90

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
0.7 %Met     (Translated Protein)
0.7 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
0.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MELRVLRYFQAVVAELNISRAAERLHVSQPTISRQLKDLEDELGATLFERNGRHISLTSS
CCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCHHHHHCCCCEEEEECC
GEYFATQANQIIALADKTLANINTAKEISGTIELGSAEMRSFLTIAQSIKKLQQQYPKIR
CCEEEECCCEEEEEECHHHHCCCCHHHCCCEEEECHHHHHHHHHHHHHHHHHHHHCCEEE
INVTSSNANQIRTNLKTGNFDFGIVTEPTDKHDLNFIHLPGESRWGLLVPRHSPLADHDY
EEEECCCCHHHEEECCCCCCCEEEEECCCCCCCCEEEECCCCCCCEEEEECCCCCCCCCC
ISLADLEGQKIIVSAQHGTINQLQDWYGESTPKFTIVATYNLLYNASLLVSAGVGYALGI
EEEEECCCCEEEEEECCCCHHHHHHHHCCCCCCEEEEEEEEHHHCCEEEEECCCCEEEEC
DGIINTNQSDLIFIPLTPRITARTSLVWTKGQRLSEAAKTFLTQLATDLQQQIPTD
CCEEECCCCCEEEEECCCCEEEEEEEEEECCHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MELRVLRYFQAVVAELNISRAAERLHVSQPTISRQLKDLEDELGATLFERNGRHISLTSS
CCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCHHHHHCCCCEEEEECC
GEYFATQANQIIALADKTLANINTAKEISGTIELGSAEMRSFLTIAQSIKKLQQQYPKIR
CCEEEECCCEEEEEECHHHHCCCCHHHCCCEEEECHHHHHHHHHHHHHHHHHHHHCCEEE
INVTSSNANQIRTNLKTGNFDFGIVTEPTDKHDLNFIHLPGESRWGLLVPRHSPLADHDY
EEEECCCCHHHEEECCCCCCCEEEEECCCCCCCCEEEECCCCCCCEEEEECCCCCCCCCC
ISLADLEGQKIIVSAQHGTINQLQDWYGESTPKFTIVATYNLLYNASLLVSAGVGYALGI
EEEEECCCCEEEEEECCCCHHHHHHHHCCCCCCEEEEEEEEHHHCCEEEEECCCCEEEEC
DGIINTNQSDLIFIPLTPRITARTSLVWTKGQRLSEAAKTFLTQLATDLQQQIPTD
CCEEECCCCCEEEEECCCCEEEEEEEEEECCHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9205837; 9278503; 9603882 [H]