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The map label for this gene is eno

Identifier: 28377645

GI number: 28377645

Start: 728191

End: 729519

Strand: Direct

Name: eno

Synonym: lp_0792

Alternate gene names: 28377645

Gene position: 728191-729519 (Clockwise)

Preceding gene: 28377644

Following gene: 28377646

Centisome position: 22.01

GC content: 44.09

Gene sequence:

>1329_bases
ATGTCTATTATTACAGATATTTATGCTCGCGAAGTCTTAGACTCACGTGGTAACCCAACTGTTGAAGTTGAACTTTATAC
TGAAAGTGGCGCATTTGGCCGCGGTATCGTTCCTTCAGGTGCCTCAACTGGTGAACATGAAGCCGTTGAATTACGTGACG
GTGACAAGAGCCGTTTCATGGGCAAGGGTGTTACTAAAGCCGTTGACAATGTTAACAAGTTAATTGCTAAGGAAATTGTT
GGTTACGATGTAACTGACCAACGTGCCATTGACCAAGCTATGATCAAGTTAGATGGTACTCCTAACAAAGCTAAGTTAGG
CGCTAACGCTATCTTAGGTGTTTCCATTGCCGCTGCACGTGCTGCTGCTGACGAACTTGAAATGCCTTTATACAACTACC
TTGGCGGATTCAACGCTCACGTTTTACCAACACCAATGATGAACGTTATCAATGGTGGGGCCCACGCCAACAACGACGTT
GACTTCCAAGAATTCATGATCATGCCTGTTGGCGCTTCTTCAGTTAAAGAAGCTATCCGGATGGGTTCAGAAACTTTCCA
CAACTTGAAAGCGATCTTGAACGAACGCGGTTACTCAACTGCCGTTGGTGACGAAGGTGGTTTTGCGCCTGACTTGAAGA
ACAACGAAGAACCATTCGAAATCTTAGTTGAAGCTATCGAACGTGCCGGTTACAAGCCTGGTAAGGATATTGCCATTGCC
TTTGACTGTGCCGCTTCAGAATTCTACAACGAAGAAACTGGCAAGTACGACTTAAAGGGTGAAGGCGAAAATGGTCAATC
ATTTACTGCCGAAGAATTCGTTGACTTACTTGACAGCATTGTTGACAAGTACCCAATCGTTTCTATCGAAGATCCTTTGG
ATGAAAACAACTGGGAAGACTGGCAAATGGCAACTGCCAAGCTTGGTAAGAAAGTTCAAATCGTTGGTGACGACTTATTC
GTTACGAACACGGACTACCTTGCAAAGGGTATCAAGATGGGCGTTGCTAACTCAATCTTAATCAAGGTTAACCAAATTGG
TACTTTAACTGAAACTGTTGAAGCTATCGAAATGGCTAAAGAAGCTGGCTACACTGCCATCGTTTCTCACCGTTCTGGTG
AAACTGAAGACACGACCATTGCTGACTTAGTTGTCGCAATGAACGCTGGCCAAATCAAGACTGGTTCAATGAGCCGTACT
GAACGGATTGCTAAATACAATCAATTAATGCGGATCGAAGACCAACTTGAAAGCACTTCAGAATACAAAGGTATCCACGG
TTTCTACAACTTAGACGAAGCTGCACGGAACACCATTACTAGCAAGTAA

Upstream 100 bases:

>100_bases
TAATTATCAAAATAACTAATAATAGTTATTGCAAGTTGATTGACAAACTTCTACAATAAGTGTGAAACAAATAACAAGCC
AATTTTAAGGAGAGAAATTA

Downstream 100 bases:

>100_bases
TTTGAAGTTCTAATGAAAACGTCGTTCCGATGGGACGGCGTTTTTATTATACATAATTTAAGGAACATCTGTTTGAGTTT
AGGGTCTAGGGACGGACGAG

Product: phosphopyruvate hydratase

Products: NA

Alternate protein names: 2-phospho-D-glycerate hydro-lyase 1; 2-phosphoglycerate dehydratase 1

Number of amino acids: Translated: 442; Mature: 441

Protein sequence:

>442_residues
MSIITDIYAREVLDSRGNPTVEVELYTESGAFGRGIVPSGASTGEHEAVELRDGDKSRFMGKGVTKAVDNVNKLIAKEIV
GYDVTDQRAIDQAMIKLDGTPNKAKLGANAILGVSIAAARAAADELEMPLYNYLGGFNAHVLPTPMMNVINGGAHANNDV
DFQEFMIMPVGASSVKEAIRMGSETFHNLKAILNERGYSTAVGDEGGFAPDLKNNEEPFEILVEAIERAGYKPGKDIAIA
FDCAASEFYNEETGKYDLKGEGENGQSFTAEEFVDLLDSIVDKYPIVSIEDPLDENNWEDWQMATAKLGKKVQIVGDDLF
VTNTDYLAKGIKMGVANSILIKVNQIGTLTETVEAIEMAKEAGYTAIVSHRSGETEDTTIADLVVAMNAGQIKTGSMSRT
ERIAKYNQLMRIEDQLESTSEYKGIHGFYNLDEAARNTITSK

Sequences:

>Translated_442_residues
MSIITDIYAREVLDSRGNPTVEVELYTESGAFGRGIVPSGASTGEHEAVELRDGDKSRFMGKGVTKAVDNVNKLIAKEIV
GYDVTDQRAIDQAMIKLDGTPNKAKLGANAILGVSIAAARAAADELEMPLYNYLGGFNAHVLPTPMMNVINGGAHANNDV
DFQEFMIMPVGASSVKEAIRMGSETFHNLKAILNERGYSTAVGDEGGFAPDLKNNEEPFEILVEAIERAGYKPGKDIAIA
FDCAASEFYNEETGKYDLKGEGENGQSFTAEEFVDLLDSIVDKYPIVSIEDPLDENNWEDWQMATAKLGKKVQIVGDDLF
VTNTDYLAKGIKMGVANSILIKVNQIGTLTETVEAIEMAKEAGYTAIVSHRSGETEDTTIADLVVAMNAGQIKTGSMSRT
ERIAKYNQLMRIEDQLESTSEYKGIHGFYNLDEAARNTITSK
>Mature_441_residues
SIITDIYAREVLDSRGNPTVEVELYTESGAFGRGIVPSGASTGEHEAVELRDGDKSRFMGKGVTKAVDNVNKLIAKEIVG
YDVTDQRAIDQAMIKLDGTPNKAKLGANAILGVSIAAARAAADELEMPLYNYLGGFNAHVLPTPMMNVINGGAHANNDVD
FQEFMIMPVGASSVKEAIRMGSETFHNLKAILNERGYSTAVGDEGGFAPDLKNNEEPFEILVEAIERAGYKPGKDIAIAF
DCAASEFYNEETGKYDLKGEGENGQSFTAEEFVDLLDSIVDKYPIVSIEDPLDENNWEDWQMATAKLGKKVQIVGDDLFV
TNTDYLAKGIKMGVANSILIKVNQIGTLTETVEAIEMAKEAGYTAIVSHRSGETEDTTIADLVVAMNAGQIKTGSMSRTE
RIAKYNQLMRIEDQLESTSEYKGIHGFYNLDEAARNTITSK

Specific function: Catalyzes the reversible conversion of 2- phosphoglycerate into phosphoenolpyruvate. It is essential for the degradation of carbohydrates via glycolysis

COG id: COG0148

COG function: function code G; Enolase

Gene ontology:

Cell location: Cytoplasm. Secreted. Cell surface. Note=Fractions of enolase are present in both the cytoplasm and on the cell surface. The export of enolase possibly depends on the covalent binding to the substrate; once secreted, it remains attached to the bacterial ce

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enolase family

Homologues:

Organism=Homo sapiens, GI5803011, Length=436, Percent_Identity=52.0642201834862, Blast_Score=439, Evalue=1e-123,
Organism=Homo sapiens, GI4503571, Length=433, Percent_Identity=52.1939953810624, Blast_Score=437, Evalue=1e-123,
Organism=Homo sapiens, GI301897477, Length=428, Percent_Identity=52.3364485981308, Blast_Score=426, Evalue=1e-119,
Organism=Homo sapiens, GI301897469, Length=428, Percent_Identity=52.3364485981308, Blast_Score=426, Evalue=1e-119,
Organism=Homo sapiens, GI301897479, Length=429, Percent_Identity=48.018648018648, Blast_Score=374, Evalue=1e-104,
Organism=Homo sapiens, GI169201331, Length=355, Percent_Identity=25.0704225352113, Blast_Score=112, Evalue=5e-25,
Organism=Homo sapiens, GI169201757, Length=355, Percent_Identity=25.0704225352113, Blast_Score=112, Evalue=5e-25,
Organism=Homo sapiens, GI239744207, Length=355, Percent_Identity=25.0704225352113, Blast_Score=112, Evalue=5e-25,
Organism=Escherichia coli, GI1789141, Length=428, Percent_Identity=60.7476635514019, Blast_Score=514, Evalue=1e-147,
Organism=Caenorhabditis elegans, GI71995829, Length=438, Percent_Identity=52.7397260273973, Blast_Score=434, Evalue=1e-122,
Organism=Caenorhabditis elegans, GI17536383, Length=438, Percent_Identity=52.7397260273973, Blast_Score=434, Evalue=1e-122,
Organism=Caenorhabditis elegans, GI32563855, Length=197, Percent_Identity=45.6852791878173, Blast_Score=179, Evalue=4e-45,
Organism=Saccharomyces cerevisiae, GI6321693, Length=433, Percent_Identity=52.8868360277136, Blast_Score=423, Evalue=1e-119,
Organism=Saccharomyces cerevisiae, GI6324974, Length=439, Percent_Identity=50.3416856492027, Blast_Score=414, Evalue=1e-116,
Organism=Saccharomyces cerevisiae, GI6324969, Length=439, Percent_Identity=50.3416856492027, Blast_Score=414, Evalue=1e-116,
Organism=Saccharomyces cerevisiae, GI6323985, Length=439, Percent_Identity=50.3416856492027, Blast_Score=414, Evalue=1e-116,
Organism=Saccharomyces cerevisiae, GI6321968, Length=433, Percent_Identity=52.4249422632794, Blast_Score=398, Evalue=1e-112,
Organism=Drosophila melanogaster, GI24580918, Length=431, Percent_Identity=51.0440835266821, Blast_Score=404, Evalue=1e-113,
Organism=Drosophila melanogaster, GI24580916, Length=431, Percent_Identity=51.0440835266821, Blast_Score=404, Evalue=1e-113,
Organism=Drosophila melanogaster, GI24580920, Length=431, Percent_Identity=51.0440835266821, Blast_Score=404, Evalue=1e-113,
Organism=Drosophila melanogaster, GI24580914, Length=431, Percent_Identity=51.0440835266821, Blast_Score=404, Evalue=1e-113,
Organism=Drosophila melanogaster, GI281360527, Length=431, Percent_Identity=51.0440835266821, Blast_Score=403, Evalue=1e-112,
Organism=Drosophila melanogaster, GI17137654, Length=431, Percent_Identity=51.0440835266821, Blast_Score=403, Evalue=1e-112,

Paralogues:

None

Copy number: 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1660 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 20 Molecules/Cell In: Stationary Phase,

Swissprot (AC and ID): ENO1_LACPL (Q88YH3)

Other databases:

- EMBL:   AL935254
- RefSeq:   NP_784537.1
- ProteinModelPortal:   Q88YH3
- SMR:   Q88YH3
- GeneID:   1063808
- GenomeReviews:   AL935263_GR
- KEGG:   lpl:lp_0792
- NMPDR:   fig|220668.1.peg.671
- HOGENOM:   HBG726599
- OMA:   DIAVGTN
- ProtClustDB:   PRK00077
- BioCyc:   LPLA220668:LP_0792-MONOMER
- BRENDA:   4.2.1.11
- GO:   GO:0006096
- HAMAP:   MF_00318
- InterPro:   IPR000941
- InterPro:   IPR020810
- InterPro:   IPR020809
- InterPro:   IPR020811
- PIRSF:   PIRSF001400
- PRINTS:   PR00148
- TIGRFAMs:   TIGR01060

Pfam domain/function: PF00113 Enolase_C; PF03952 Enolase_N

EC number: =4.2.1.11

Molecular weight: Translated: 48030; Mature: 47899

Theoretical pI: Translated: 4.34; Mature: 4.34

Prosite motif: PS00164 ENOLASE

Important sites: ACT_SITE 205-205 ACT_SITE 342-342 BINDING 155-155 BINDING 164-164 BINDING 290-290 BINDING 317-317 BINDING 342-342 BINDING 393-393

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSIITDIYAREVLDSRGNPTVEVELYTESGAFGRGIVPSGASTGEHEAVELRDGDKSRFM
CCHHHHHHHHHHHHCCCCCEEEEEEEECCCCCCCCCCCCCCCCCCCCEEEECCCCHHHHH
GKGVTKAVDNVNKLIAKEIVGYDVTDQRAIDQAMIKLDGTPNKAKLGANAILGVSIAAAR
HCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHEECCCCCHHHCCCCHHHHHHHHHHH
AAADELEMPLYNYLGGFNAHVLPTPMMNVINGGAHANNDVDFQEFMIMPVGASSVKEAIR
HHHHHHCCCHHHHHCCCCCCCCCCHHHHHHCCCCCCCCCCCHHHEEEEECCHHHHHHHHH
MGSETFHNLKAILNERGYSTAVGDEGGFAPDLKNNEEPFEILVEAIERAGYKPGKDIAIA
HCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCEEEE
FDCAASEFYNEETGKYDLKGEGENGQSFTAEEFVDLLDSIVDKYPIVSIEDPLDENNWED
EECHHHHHHCCCCCCEEECCCCCCCCCCCHHHHHHHHHHHHHHCCEEEECCCCCCCCCHH
WQMATAKLGKKVQIVGDDLFVTNTDYLAKGIKMGVANSILIKVNQIGTLTETVEAIEMAK
HHHHHHHHCCEEEEEECCEEEECHHHHHHHHHHCCCCEEEEEEECCCCHHHHHHHHHHHH
EAGYTAIVSHRSGETEDTTIADLVVAMNAGQIKTGSMSRTERIAKYNQLMRIEDQLESTS
HCCCEEEEECCCCCCCCHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
EYKGIHGFYNLDEAARNTITSK
HCCCCCCCCCHHHHHHHHCCCC
>Mature Secondary Structure 
SIITDIYAREVLDSRGNPTVEVELYTESGAFGRGIVPSGASTGEHEAVELRDGDKSRFM
CHHHHHHHHHHHHCCCCCEEEEEEEECCCCCCCCCCCCCCCCCCCCEEEECCCCHHHHH
GKGVTKAVDNVNKLIAKEIVGYDVTDQRAIDQAMIKLDGTPNKAKLGANAILGVSIAAAR
HCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHEECCCCCHHHCCCCHHHHHHHHHHH
AAADELEMPLYNYLGGFNAHVLPTPMMNVINGGAHANNDVDFQEFMIMPVGASSVKEAIR
HHHHHHCCCHHHHHCCCCCCCCCCHHHHHHCCCCCCCCCCCHHHEEEEECCHHHHHHHHH
MGSETFHNLKAILNERGYSTAVGDEGGFAPDLKNNEEPFEILVEAIERAGYKPGKDIAIA
HCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCEEEE
FDCAASEFYNEETGKYDLKGEGENGQSFTAEEFVDLLDSIVDKYPIVSIEDPLDENNWED
EECHHHHHHCCCCCCEEECCCCCCCCCCCHHHHHHHHHHHHHHCCEEEECCCCCCCCCHH
WQMATAKLGKKVQIVGDDLFVTNTDYLAKGIKMGVANSILIKVNQIGTLTETVEAIEMAK
HHHHHHHHCCEEEEEECCEEEECHHHHHHHHHHCCCCEEEEEEECCCCHHHHHHHHHHHH
EAGYTAIVSHRSGETEDTTIADLVVAMNAGQIKTGSMSRTERIAKYNQLMRIEDQLESTS
HCCCEEEEECCCCCCCCHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
EYKGIHGFYNLDEAARNTITSK
HCCCCCCCCCHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12566566