Click here to switch to the map view.

The map label for this gene is prfB

Identifier: 28377601

GI number: 28377601

Start: 678084

End: 679157

Strand: Direct

Name: prfB

Synonym: lp_0741

Alternate gene names: 28377601

Gene position: 678084-679157 (Clockwise)

Preceding gene: 28377600

Following gene: 28377602

Centisome position: 20.5

GC content: 47.86

Gene sequence:

>1074_bases
GTGCTGTCGACGACTTTAGGGGGTCGCTTTGACTTAGATGCTTTGAACGAAAGCATTCAAGAAAATGAAGCTCGAATGGC
CGAACCCGGTTTTTGGGACGACCAGGCTGCCGCCCAGAAAGTCATCGATGAGAATAACGTGTTGAAAGGAAAGTATGATA
CGTTTAAGCAATTAGCTGATGAAGTCGGTGACTTGGCAGTCGCTTATGAGTTACTGAGTGAGGAACCCGATGCTGAGATG
CAGGCTGAGTTTGAAACGGACTTTCAGCACGCTGAACATGATTTACAACAGTACCGGCTCAACTTACTTTTGGACGGTCC
GTACGACCGCAATAACGCTATTTTAGAAATCCATCCTGGAGCTGGTGGGACCGAATCCCAAGATTGGGGTGCGATGCTAC
TAAGAATGTACACGCGCTGGGCTGCGAGTCACAATTTCAAAGTCGAGACTGTTGACTACCAAGCTGGTGATGAGGCAGGC
ATTAAGAGTGTGACTTTATTAATTAGCGGCCACAATGCTTACGGTTATTTGCGGTCAGAAAAAGGGGTTCATCGTCTTGT
TCGGATTTCGCCGTTCGATGCGGCTGGTCGGCGCCACACATCTTTTGCGAGTGTTGATGTGATGCCTGAGTTAGATGATA
CCGTGGATGTTGATATTCGACCTGAAGATTTGAAGATTGACGTTTATCGAGCCAGTGGGGCCGGTGGACAACATGTCAAT
AAGACTTCTTCAGCGGTTCGGATTACCCACGTGCCAACGGGAATTGTCGTTGCCAGTCAAGCGCAACGGTCACAATTACA
GAACCGACAAACAGCATTGAACATGTTACGAGCGAAGTTGTACGAACGTGAAGAAGAGAAGAAGGCTAAGGAACGGGCGG
CCATCCAAGGTGAGCAAATGGACATTGGGTGGGGCTCACAAATTCGCTCCTACGTTTTTCATCCGTATACGATGGTCAAG
GATCACCGGACTAATTATGAATCACATCATGGGCAGGCTGTCATGGATGGTGATCTTGATCCGTTTATGGATGCTTACTT
ACAATGGAAATTAGCCCAACGTAATCCGCAATAG

Upstream 100 bases:

>100_bases
TTTACAGTTAAACCAGATATTAAATAATTAATAAATAAGTTGAAAGAAGGGTCCTTTGTGGAATTAAGTGAATATAAACA
CCTGATTGAAGAAATGCAAA

Downstream 100 bases:

>100_bases
ATTGCACAAATACTAAGGTGACCTATCATTGCGCAGGTCGTCTTAGTATTTTTTTTGCTACTAGTCTTATAATATAGAGA
TATTAAAGCGGTAATAGCAG

Product: peptide chain release factor 2

Products: NA

Alternate protein names: RF-2

Number of amino acids: Translated: 357; Mature: 357

Protein sequence:

>357_residues
MLSTTLGGRFDLDALNESIQENEARMAEPGFWDDQAAAQKVIDENNVLKGKYDTFKQLADEVGDLAVAYELLSEEPDAEM
QAEFETDFQHAEHDLQQYRLNLLLDGPYDRNNAILEIHPGAGGTESQDWGAMLLRMYTRWAASHNFKVETVDYQAGDEAG
IKSVTLLISGHNAYGYLRSEKGVHRLVRISPFDAAGRRHTSFASVDVMPELDDTVDVDIRPEDLKIDVYRASGAGGQHVN
KTSSAVRITHVPTGIVVASQAQRSQLQNRQTALNMLRAKLYEREEEKKAKERAAIQGEQMDIGWGSQIRSYVFHPYTMVK
DHRTNYESHHGQAVMDGDLDPFMDAYLQWKLAQRNPQ

Sequences:

>Translated_357_residues
MLSTTLGGRFDLDALNESIQENEARMAEPGFWDDQAAAQKVIDENNVLKGKYDTFKQLADEVGDLAVAYELLSEEPDAEM
QAEFETDFQHAEHDLQQYRLNLLLDGPYDRNNAILEIHPGAGGTESQDWGAMLLRMYTRWAASHNFKVETVDYQAGDEAG
IKSVTLLISGHNAYGYLRSEKGVHRLVRISPFDAAGRRHTSFASVDVMPELDDTVDVDIRPEDLKIDVYRASGAGGQHVN
KTSSAVRITHVPTGIVVASQAQRSQLQNRQTALNMLRAKLYEREEEKKAKERAAIQGEQMDIGWGSQIRSYVFHPYTMVK
DHRTNYESHHGQAVMDGDLDPFMDAYLQWKLAQRNPQ
>Mature_357_residues
MLSTTLGGRFDLDALNESIQENEARMAEPGFWDDQAAAQKVIDENNVLKGKYDTFKQLADEVGDLAVAYELLSEEPDAEM
QAEFETDFQHAEHDLQQYRLNLLLDGPYDRNNAILEIHPGAGGTESQDWGAMLLRMYTRWAASHNFKVETVDYQAGDEAG
IKSVTLLISGHNAYGYLRSEKGVHRLVRISPFDAAGRRHTSFASVDVMPELDDTVDVDIRPEDLKIDVYRASGAGGQHVN
KTSSAVRITHVPTGIVVASQAQRSQLQNRQTALNMLRAKLYEREEEKKAKERAAIQGEQMDIGWGSQIRSYVFHPYTMVK
DHRTNYESHHGQAVMDGDLDPFMDAYLQWKLAQRNPQ

Specific function: Peptide chain release factor 2 directs the termination of translation in response to the peptide chain termination codons UGA and UAA

COG id: COG1186

COG function: function code J; Protein chain release factor B

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic/mitochondrial release factor family

Homologues:

Organism=Homo sapiens, GI166795303, Length=268, Percent_Identity=38.0597014925373, Blast_Score=173, Evalue=2e-43,
Organism=Homo sapiens, GI34577120, Length=251, Percent_Identity=37.8486055776892, Blast_Score=163, Evalue=3e-40,
Organism=Homo sapiens, GI166795305, Length=182, Percent_Identity=37.9120879120879, Blast_Score=121, Evalue=8e-28,
Organism=Escherichia coli, GI2367172, Length=346, Percent_Identity=48.8439306358381, Blast_Score=332, Evalue=3e-92,
Organism=Escherichia coli, GI1787462, Length=243, Percent_Identity=42.3868312757202, Blast_Score=189, Evalue=3e-49,
Organism=Caenorhabditis elegans, GI17542784, Length=255, Percent_Identity=32.5490196078431, Blast_Score=111, Evalue=5e-25,
Organism=Saccharomyces cerevisiae, GI6321295, Length=319, Percent_Identity=32.9153605015674, Blast_Score=156, Evalue=5e-39,
Organism=Drosophila melanogaster, GI19921226, Length=211, Percent_Identity=40.2843601895735, Blast_Score=154, Evalue=7e-38,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RF2_LACPL (Q88YL5)

Other databases:

- EMBL:   AL935254
- RefSeq:   NP_784493.1
- ProteinModelPortal:   Q88YL5
- SMR:   Q88YL5
- GeneID:   1063494
- GenomeReviews:   AL935263_GR
- KEGG:   lpl:lp_0741
- NMPDR:   fig|220668.1.peg.627
- HOGENOM:   HBG629764
- OMA:   TEMLLRM
- ProtClustDB:   PRK00578
- BioCyc:   LPLA220668:LP_0741-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00094
- InterPro:   IPR005139
- InterPro:   IPR000352
- InterPro:   IPR020853
- InterPro:   IPR004374
- PANTHER:   PTHR11075:SF6
- SMART:   SM00937
- TIGRFAMs:   TIGR00020

Pfam domain/function: PF03462 PCRF; PF00472 RF-1

EC number: NA

Molecular weight: Translated: 40259; Mature: 40259

Theoretical pI: Translated: 4.84; Mature: 4.84

Prosite motif: PS00745 RF_PROK_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLSTTLGGRFDLDALNESIQENEARMAEPGFWDDQAAAQKVIDENNVLKGKYDTFKQLAD
CCCCCCCCEECHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCEECCCHHHHHHHHH
EVGDLAVAYELLSEEPDAEMQAEFETDFQHAEHDLQQYRLNLLLDGPYDRNNAILEIHPG
HHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHEEEEEEECCCCCCCEEEEEECC
AGGTESQDWGAMLLRMYTRWAASHNFKVETVDYQAGDEAGIKSVTLLISGHNAYGYLRSE
CCCCCCCHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCEEEEEEEECCCCEEEEECC
KGVHRLVRISPFDAAGRRHTSFASVDVMPELDDTVDVDIRPEDLKIDVYRASGAGGQHVN
CCCEEEEEECCCCCCCCCCCCEEEEECCCCCCCCEEEEECCCCEEEEEEEECCCCCCCCC
KTSSAVRITHVPTGIVVASQAQRSQLQNRQTALNMLRAKLYEREEEKKAKERAAIQGEQM
CCCCEEEEEECCCCEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCE
DIGWGSQIRSYVFHPYTMVKDHRTNYESHHGQAVMDGDLDPFMDAYLQWKLAQRNPQ
ECCCHHHHHHHHCCCHHEECCCCCCCCCCCCCEEECCCCCHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MLSTTLGGRFDLDALNESIQENEARMAEPGFWDDQAAAQKVIDENNVLKGKYDTFKQLAD
CCCCCCCCEECHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCEECCCHHHHHHHHH
EVGDLAVAYELLSEEPDAEMQAEFETDFQHAEHDLQQYRLNLLLDGPYDRNNAILEIHPG
HHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHEEEEEEECCCCCCCEEEEEECC
AGGTESQDWGAMLLRMYTRWAASHNFKVETVDYQAGDEAGIKSVTLLISGHNAYGYLRSE
CCCCCCCHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCEEEEEEEECCCCEEEEECC
KGVHRLVRISPFDAAGRRHTSFASVDVMPELDDTVDVDIRPEDLKIDVYRASGAGGQHVN
CCCEEEEEECCCCCCCCCCCCEEEEECCCCCCCCEEEEECCCCEEEEEEEECCCCCCCCC
KTSSAVRITHVPTGIVVASQAQRSQLQNRQTALNMLRAKLYEREEEKKAKERAAIQGEQM
CCCCEEEEEECCCCEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCE
DIGWGSQIRSYVFHPYTMVKDHRTNYESHHGQAVMDGDLDPFMDAYLQWKLAQRNPQ
ECCCHHHHHHHHCCCHHEECCCCCCCCCCCCCEEECCCCCHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12566566