Click here to switch to the map view.

The map label for this gene is proC [H]

Identifier: 28377443

GI number: 28377443

Start: 516781

End: 517572

Strand: Direct

Name: proC [H]

Synonym: lp_0561

Alternate gene names: 28377443

Gene position: 516781-517572 (Clockwise)

Preceding gene: 28377440

Following gene: 28377444

Centisome position: 15.62

GC content: 50.25

Gene sequence:

>792_bases
ATGAAAATCGGATTCATTGGTGCCGGTAACATGGGCCGCGCGATTATTGATGGGTGGTTAAAACAACAAGCGGTCGTCCC
AGCAGATATTTATATTCACAGTGCCCATGCCGCGAGTTATCAACCTTATGCGCAAAAAAATGGGCTACAGGCCTGTGACA
CTAATTTGGCCGTGGCACAAGCTGCTGACGTTATCGTTTTGGCAGTTAAGCCGAACATTGCGTTGGCCGCATTAAAAGAA
GTTCAAGCAGCCTTAACGGGCAAGTGGGTTATTACAATGGTATCAGGGTTGAGTTTGGCCGATTATGCCACGGTTGCCCC
TGATCTCCCCGTTCTGAGAATCATGCCGAATGTGAACGTGGCGATTGGTGCCGGGATGACGGCGTTGGTTGGTAATGATG
CCTTAACACCAGAGCAATATGCGGCTGGGCAACAATTATTCGATGCGATTGGTGCCACGAGTGCCATCGCTGAGAAAGAT
TTTCCAACTTTTTCAGCCTTAGCAGGTAGTTCGCCGGCCTACATCTATTTCTTTATTGACGCGATGGCCCGCGCAGGCGT
CAAGCACGGTTTGAGCAAGGATGCAGCGACTAAGATTGCGGCGCAGGCCACGCTGGGCAGTGCGCAGAATGTTTTAGCTT
CTGATAAAATTCCGTTTGACCTGATCGATCAAGTTTCTTCACCTGGTGGCACGACGGTTGCTGGATTACTGGCCATGGAA
GAGGCCGGCTTTATGACCGCAGTGGTGAAGGGAATTGATGCAACGATTGCGAAGGAACTTGGCGAATCTTAA

Upstream 100 bases:

>100_bases
ATAGTAGAAGAGTAAGCGGGAATGTCTGTTTAATCCTGCCTAGCCGTTAATCGGTTAGTGGTTAATGATATAAATTATAA
GTCAGAGGAGCGTTTTACGG

Downstream 100 bases:

>100_bases
ATTACTTGGTGCTGGTGGTTGCAAGTTTGGTCTATACCGATTATAATTAAACCAGATAAATCCAAGGAGGAATTGTTCAT
GAGCAAAGTGTTAAAACATG

Product: pyrroline-5-carboxylate reductase

Products: NA

Alternate protein names: P5C reductase; P5CR [H]

Number of amino acids: Translated: 263; Mature: 263

Protein sequence:

>263_residues
MKIGFIGAGNMGRAIIDGWLKQQAVVPADIYIHSAHAASYQPYAQKNGLQACDTNLAVAQAADVIVLAVKPNIALAALKE
VQAALTGKWVITMVSGLSLADYATVAPDLPVLRIMPNVNVAIGAGMTALVGNDALTPEQYAAGQQLFDAIGATSAIAEKD
FPTFSALAGSSPAYIYFFIDAMARAGVKHGLSKDAATKIAAQATLGSAQNVLASDKIPFDLIDQVSSPGGTTVAGLLAME
EAGFMTAVVKGIDATIAKELGES

Sequences:

>Translated_263_residues
MKIGFIGAGNMGRAIIDGWLKQQAVVPADIYIHSAHAASYQPYAQKNGLQACDTNLAVAQAADVIVLAVKPNIALAALKE
VQAALTGKWVITMVSGLSLADYATVAPDLPVLRIMPNVNVAIGAGMTALVGNDALTPEQYAAGQQLFDAIGATSAIAEKD
FPTFSALAGSSPAYIYFFIDAMARAGVKHGLSKDAATKIAAQATLGSAQNVLASDKIPFDLIDQVSSPGGTTVAGLLAME
EAGFMTAVVKGIDATIAKELGES
>Mature_263_residues
MKIGFIGAGNMGRAIIDGWLKQQAVVPADIYIHSAHAASYQPYAQKNGLQACDTNLAVAQAADVIVLAVKPNIALAALKE
VQAALTGKWVITMVSGLSLADYATVAPDLPVLRIMPNVNVAIGAGMTALVGNDALTPEQYAAGQQLFDAIGATSAIAEKD
FPTFSALAGSSPAYIYFFIDAMARAGVKHGLSKDAATKIAAQATLGSAQNVLASDKIPFDLIDQVSSPGGTTVAGLLAME
EAGFMTAVVKGIDATIAKELGES

Specific function: Proline biosynthesis; third (last) step. [C]

COG id: COG0345

COG function: function code E; Pyrroline-5-carboxylate reductase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pyrroline-5-carboxylate reductase family [H]

Homologues:

Organism=Homo sapiens, GI198041662, Length=263, Percent_Identity=30.4182509505703, Blast_Score=119, Evalue=2e-27,
Organism=Homo sapiens, GI24797095, Length=264, Percent_Identity=30.3030303030303, Blast_Score=116, Evalue=3e-26,
Organism=Homo sapiens, GI24797097, Length=264, Percent_Identity=30.3030303030303, Blast_Score=115, Evalue=3e-26,
Organism=Homo sapiens, GI21361454, Length=266, Percent_Identity=30.4511278195489, Blast_Score=114, Evalue=8e-26,
Organism=Escherichia coli, GI1786585, Length=259, Percent_Identity=37.4517374517375, Blast_Score=167, Evalue=4e-43,
Organism=Caenorhabditis elegans, GI17569021, Length=257, Percent_Identity=30.3501945525292, Blast_Score=115, Evalue=3e-26,
Organism=Caenorhabditis elegans, GI17540664, Length=280, Percent_Identity=30.7142857142857, Blast_Score=110, Evalue=5e-25,
Organism=Saccharomyces cerevisiae, GI6320861, Length=224, Percent_Identity=28.5714285714286, Blast_Score=101, Evalue=1e-22,
Organism=Drosophila melanogaster, GI21358587, Length=250, Percent_Identity=32.4, Blast_Score=140, Evalue=1e-33,
Organism=Drosophila melanogaster, GI24648116, Length=274, Percent_Identity=30.2919708029197, Blast_Score=135, Evalue=2e-32,
Organism=Drosophila melanogaster, GI24647700, Length=146, Percent_Identity=32.8767123287671, Blast_Score=91, Evalue=9e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008927
- InterPro:   IPR016040
- InterPro:   IPR004455
- InterPro:   IPR000304 [H]

Pfam domain/function: PF03807 F420_oxidored [H]

EC number: =1.5.1.2 [H]

Molecular weight: Translated: 26993; Mature: 26993

Theoretical pI: Translated: 4.77; Mature: 4.77

Prosite motif: PS00521 P5CR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIGFIGAGNMGRAIIDGWLKQQAVVPADIYIHSAHAASYQPYAQKNGLQACDTNLAVAQ
CEEEEEECCCCCHHHHHHHHHHCCCCCHHEEEECCCCCCCCCHHHHCCCCHHCCCHHHEE
AADVIVLAVKPNIALAALKEVQAALTGKWVITMVSGLSLADYATVAPDLPVLRIMPNVNV
CCCEEEEEECCCHHHHHHHHHHHHHCCHHHHHHHCCCCHHHHHHCCCCCCEEEECCCCCE
AIGAGMTALVGNDALTPEQYAAGQQLFDAIGATSAIAEKDFPTFSALAGSSPAYIYFFID
EECCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHCCCCCEEEEHHHH
AMARAGVKHGLSKDAATKIAAQATLGSAQNVLASDKIPFDLIDQVSSPGGTTVAGLLAME
HHHHHHHHHCCCHHHHHHHHHHHHHCCHHHHHHCCCCCHHHHHHHCCCCCCHHHHHHHHH
EAGFMTAVVKGIDATIAKELGES
HCCHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MKIGFIGAGNMGRAIIDGWLKQQAVVPADIYIHSAHAASYQPYAQKNGLQACDTNLAVAQ
CEEEEEECCCCCHHHHHHHHHHCCCCCHHEEEECCCCCCCCCHHHHCCCCHHCCCHHHEE
AADVIVLAVKPNIALAALKEVQAALTGKWVITMVSGLSLADYATVAPDLPVLRIMPNVNV
CCCEEEEEECCCHHHHHHHHHHHHHCCHHHHHHHCCCCHHHHHHCCCCCCEEEECCCCCE
AIGAGMTALVGNDALTPEQYAAGQQLFDAIGATSAIAEKDFPTFSALAGSSPAYIYFFID
EECCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHCCCCCEEEEHHHH
AMARAGVKHGLSKDAATKIAAQATLGSAQNVLASDKIPFDLIDQVSSPGGTTVAGLLAME
HHHHHHHHHCCCHHHHHHHHHHHHHCCHHHHHHCCCCCHHHHHHHCCCCCCHHHHHHHHH
EAGFMTAVVKGIDATIAKELGES
HCCHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 6296787; 9278503 [H]