Click here to switch to the map view.

The map label for this gene is phs [H]

Identifier: 28199692

GI number: 28199692

Start: 2125604

End: 2127103

Strand: Reverse

Name: phs [H]

Synonym: PD1822

Alternate gene names: 28199692

Gene position: 2127103-2125604 (Counterclockwise)

Preceding gene: 28199693

Following gene: 28199686

Centisome position: 84.42

GC content: 53.8

Gene sequence:

>1500_bases
GTGCAGATAGTGTTGGATGACGGTCAACCGGTTGACGGTATATTCCGTTTGACCAATGCCAGCTTTGGTCGCCATCTCCG
GCTTGATTCGGTGGTTGGGGAGTCCGTGTGCGGCATCATGCCTGACGATGGGCCGGAATACATTAAGCGTTATGGTGGGG
TTGCGTTGTCTGGGCAGCGGGTTCACTTCGAGGCGCGGGCGTTGCGCCGTTGGTATTCGGTGGAAGTGATGCGTGTTGGA
GCGCCGGAGGGGCGATTGCTTGGGGTTCTATTTGTGGATATCACCGAGCACAAGCGTATGGAACGTAGGTTGGCCGAGAG
CGAGGTGCGTTTCAATATTTTGGCTGATGGTTTGCCGATGCCCGTTTGGGTATTTGATGCACAAGGAGGGATGCGGTTCG
TCAATACTGCGTATGGGGAGTTCTTCGGTGTCGATTTGAGCAGTGGCACGCTTCCAGAGTGGAGTATGGTGCTGCATCCG
GAGGATGTGCTGATATTCGAGTTCAAGTTGAGTGCGGCGCTGAAGACGCAGAGTGCGCTTCGTGCGTTGGTACGGGCACG
TCGTTATGACGGGCAATGGCGTTGGATTGAAATGAGTGCCACGCCGCGTTATTCGCCGGATGGTCACTTCATCGGTCTGA
CGGGGAGCAGTCAGGATGTGACGGAGCGTCTTGAGATTGAGTTGGCGCGTGAGCAGTTGCTGAAATCCGAGCGTGCGGCG
CGCAATGAGGCCGAGAATATGGCGCGATTGAAGGATGAGTTTCTGTCCACGCTTTCGCATGAGTTACGTACCCCGTTAAC
GACGATTTTGGGTTGGAGTGAGTTGTTGTTGCAACGCATTGAGGAGCAGCATCCTTGTTACAAAGGGTTGTCGGTGATTG
CTAGCAGCGCCAAGGTTCAAAAGCGTTTGATTTCGGATATGTTGGATCTAAGTAGCATGCTGCTGGGCAAGATGCAGTTG
GAAATGGAGGTGCTGGATTTAGTGGAGCAGATCCGCGAGGTGATTGGGTTGTATGAGCAGGTTGTCGAGAGCAAGGGGCA
GCGGTTGCTGCTGCGCGCGCCGGAGATGCCGTGTTTGGTGTTGGGTGATGCAACGCGCTTGCGGCAGATATTCGAAAATT
TGTTGTCTAATGCGATCAAATTTACTCCAACGGATGGCTGCATTGATGTGGAGATTGATGCCGATGGCGACAGTTTCCGG
GTATCGGTGACGGATTCTGGTGATGGGATCGCTGCCGAATTTCTACCGCATTTGTTTAGTCGGTTTCGTCAGGCTGATGG
TACGACGACCCGTTGTCACGGTGGGCTTGGTCTGGGGTTGGCGATTGTGCAGAGTTTGGTGGAAATGCATGGGGGTTATG
TTGGTGCGACCAGTCCGGGGCTTGGTAAGGGTGCAATGTTCACGGTGTCTTTGTTGCGTTATGTATGTGCGATCAATGTT
GCCGATACTGTGATCTGTGATCGGCGTTTTGGATCAGTAGGCGACCAAGGGGAGTATTGA

Upstream 100 bases:

>100_bases
TCTTTTGGACATATCGCCTCCGCTTGTCCTGCAGTCACTCGACATCCCATCGGATGTCTACCGGGAGATGCTGTGTACCC
TTGAGGCTGGCTTTTGCATC

Downstream 100 bases:

>100_bases
AGTGATTTCATTGTTCTGGTTGTACCAGGATGCATTTGCAAAATGCCGTTTCCAGGTTGGATGCAGCATTGCCGTTCAAT
GTCATTGTGTGGCCGGTGTC

Product: histidine kinase/response regulator hybrid protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 499; Mature: 499

Protein sequence:

>499_residues
MQIVLDDGQPVDGIFRLTNASFGRHLRLDSVVGESVCGIMPDDGPEYIKRYGGVALSGQRVHFEARALRRWYSVEVMRVG
APEGRLLGVLFVDITEHKRMERRLAESEVRFNILADGLPMPVWVFDAQGGMRFVNTAYGEFFGVDLSSGTLPEWSMVLHP
EDVLIFEFKLSAALKTQSALRALVRARRYDGQWRWIEMSATPRYSPDGHFIGLTGSSQDVTERLEIELAREQLLKSERAA
RNEAENMARLKDEFLSTLSHELRTPLTTILGWSELLLQRIEEQHPCYKGLSVIASSAKVQKRLISDMLDLSSMLLGKMQL
EMEVLDLVEQIREVIGLYEQVVESKGQRLLLRAPEMPCLVLGDATRLRQIFENLLSNAIKFTPTDGCIDVEIDADGDSFR
VSVTDSGDGIAAEFLPHLFSRFRQADGTTTRCHGGLGLGLAIVQSLVEMHGGYVGATSPGLGKGAMFTVSLLRYVCAINV
ADTVICDRRFGSVGDQGEY

Sequences:

>Translated_499_residues
MQIVLDDGQPVDGIFRLTNASFGRHLRLDSVVGESVCGIMPDDGPEYIKRYGGVALSGQRVHFEARALRRWYSVEVMRVG
APEGRLLGVLFVDITEHKRMERRLAESEVRFNILADGLPMPVWVFDAQGGMRFVNTAYGEFFGVDLSSGTLPEWSMVLHP
EDVLIFEFKLSAALKTQSALRALVRARRYDGQWRWIEMSATPRYSPDGHFIGLTGSSQDVTERLEIELAREQLLKSERAA
RNEAENMARLKDEFLSTLSHELRTPLTTILGWSELLLQRIEEQHPCYKGLSVIASSAKVQKRLISDMLDLSSMLLGKMQL
EMEVLDLVEQIREVIGLYEQVVESKGQRLLLRAPEMPCLVLGDATRLRQIFENLLSNAIKFTPTDGCIDVEIDADGDSFR
VSVTDSGDGIAAEFLPHLFSRFRQADGTTTRCHGGLGLGLAIVQSLVEMHGGYVGATSPGLGKGAMFTVSLLRYVCAINV
ADTVICDRRFGSVGDQGEY
>Mature_499_residues
MQIVLDDGQPVDGIFRLTNASFGRHLRLDSVVGESVCGIMPDDGPEYIKRYGGVALSGQRVHFEARALRRWYSVEVMRVG
APEGRLLGVLFVDITEHKRMERRLAESEVRFNILADGLPMPVWVFDAQGGMRFVNTAYGEFFGVDLSSGTLPEWSMVLHP
EDVLIFEFKLSAALKTQSALRALVRARRYDGQWRWIEMSATPRYSPDGHFIGLTGSSQDVTERLEIELAREQLLKSERAA
RNEAENMARLKDEFLSTLSHELRTPLTTILGWSELLLQRIEEQHPCYKGLSVIASSAKVQKRLISDMLDLSSMLLGKMQL
EMEVLDLVEQIREVIGLYEQVVESKGQRLLLRAPEMPCLVLGDATRLRQIFENLLSNAIKFTPTDGCIDVEIDADGDSFR
VSVTDSGDGIAAEFLPHLFSRFRQADGTTTRCHGGLGLGLAIVQSLVEMHGGYVGATSPGLGKGAMFTVSLLRYVCAINV
ADTVICDRRFGSVGDQGEY

Specific function: Member of the two-component regulatory system phoP/phoR involved in the alkaline phosphatase genes regulation. PhoR may function as a membrane-associated protein kinase that phosphorylates phoP in response to environmental signals [H]

COG id: COG0642

COG function: function code T; Signal transduction histidine kinase

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PAS (PER-ARNT-SIM) domain [H]

Homologues:

Organism=Escherichia coli, GI1789149, Length=266, Percent_Identity=36.8421052631579, Blast_Score=134, Evalue=1e-32,
Organism=Escherichia coli, GI145693157, Length=258, Percent_Identity=35.2713178294574, Blast_Score=128, Evalue=1e-30,
Organism=Escherichia coli, GI48994928, Length=296, Percent_Identity=34.7972972972973, Blast_Score=127, Evalue=2e-30,
Organism=Escherichia coli, GI1786783, Length=263, Percent_Identity=31.9391634980989, Blast_Score=119, Evalue=5e-28,
Organism=Escherichia coli, GI1788713, Length=383, Percent_Identity=27.154046997389, Blast_Score=115, Evalue=6e-27,
Organism=Escherichia coli, GI87081816, Length=262, Percent_Identity=34.3511450381679, Blast_Score=112, Evalue=5e-26,
Organism=Escherichia coli, GI1788393, Length=227, Percent_Identity=29.5154185022026, Blast_Score=109, Evalue=5e-25,
Organism=Escherichia coli, GI1786912, Length=245, Percent_Identity=31.4285714285714, Blast_Score=103, Evalue=3e-23,
Organism=Escherichia coli, GI1790436, Length=244, Percent_Identity=31.1475409836066, Blast_Score=101, Evalue=1e-22,
Organism=Escherichia coli, GI1786600, Length=229, Percent_Identity=31.0043668122271, Blast_Score=97, Evalue=3e-21,
Organism=Escherichia coli, GI1790346, Length=218, Percent_Identity=32.1100917431193, Blast_Score=95, Evalue=1e-20,
Organism=Escherichia coli, GI1790861, Length=210, Percent_Identity=27.6190476190476, Blast_Score=73, Evalue=4e-14,
Organism=Escherichia coli, GI1788279, Length=298, Percent_Identity=23.1543624161074, Blast_Score=73, Evalue=5e-14,
Organism=Escherichia coli, GI1788549, Length=288, Percent_Identity=28.125, Blast_Score=66, Evalue=6e-12,
Organism=Escherichia coli, GI1787894, Length=245, Percent_Identity=27.3469387755102, Blast_Score=65, Evalue=9e-12,
Organism=Escherichia coli, GI1789403, Length=205, Percent_Identity=30.7317073170732, Blast_Score=64, Evalue=2e-11,
Organism=Saccharomyces cerevisiae, GI6322044, Length=150, Percent_Identity=32.6666666666667, Blast_Score=70, Evalue=7e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR000014
- InterPro:   IPR013767
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF02518 HATPase_c; PF00512 HisKA; PF00989 PAS [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 55539; Mature: 55539

Theoretical pI: Translated: 5.18; Mature: 5.18

Prosite motif: PS50112 PAS ; PS50113 PAC ; PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQIVLDDGQPVDGIFRLTNASFGRHLRLDSVVGESVCGIMPDDGPEYIKRYGGVALSGQR
CEEEECCCCCCCHHHEECCCCCCCEEEHHHHHCCHHCCCCCCCCHHHHHHHCCEEECCCE
VHFEARALRRWYSVEVMRVGAPEGRLLGVLFVDITEHKRMERRLAESEVRFNILADGLPM
EEHHHHHHHHHHEEEEEEECCCCCCEEEEEEEEEHHHHHHHHHHHHHHHEEEEEECCCCC
PVWVFDAQGGMRFVNTAYGEFFGVDLSSGTLPEWSMVLHPEDVLIFEFKLSAALKTQSAL
CEEEEECCCCCHHHHHHHHHHEEEECCCCCCCCCEEEECCCCEEEEEEEHHHHHHHHHHH
RALVRARRYDGQWRWIEMSATPRYSPDGHFIGLTGSSQDVTERLEIELAREQLLKSERAA
HHHHHHHHCCCCEEEEEECCCCCCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHH
RNEAENMARLKDEFLSTLSHELRTPLTTILGWSELLLQRIEEQHPCYKGLSVIASSAKVQ
HHHHHHHHHHHHHHHHHHHHHHHCHHHHHHCHHHHHHHHHHHHCCHHHHHHHHHHHHHHH
KRLISDMLDLSSMLLGKMQLEMEVLDLVEQIREVIGLYEQVVESKGQRLLLRAPEMPCLV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCEEE
LGDATRLRQIFENLLSNAIKFTPTDGCIDVEIDADGDSFRVSVTDSGDGIAAEFLPHLFS
ECCHHHHHHHHHHHHHCCEEECCCCCEEEEEEECCCCEEEEEEECCCCCHHHHHHHHHHH
RFRQADGTTTRCHGGLGLGLAIVQSLVEMHGGYVGATSPGLGKGAMFTVSLLRYVCAINV
HHHHCCCCCEEECCCCCHHHHHHHHHHHHCCCEEECCCCCCCCCHHHHHHHHHHHHHCCH
ADTVICDRRFGSVGDQGEY
HHHHHCCCCCCCCCCCCCC
>Mature Secondary Structure
MQIVLDDGQPVDGIFRLTNASFGRHLRLDSVVGESVCGIMPDDGPEYIKRYGGVALSGQR
CEEEECCCCCCCHHHEECCCCCCCEEEHHHHHCCHHCCCCCCCCHHHHHHHCCEEECCCE
VHFEARALRRWYSVEVMRVGAPEGRLLGVLFVDITEHKRMERRLAESEVRFNILADGLPM
EEHHHHHHHHHHEEEEEEECCCCCCEEEEEEEEEHHHHHHHHHHHHHHHEEEEEECCCCC
PVWVFDAQGGMRFVNTAYGEFFGVDLSSGTLPEWSMVLHPEDVLIFEFKLSAALKTQSAL
CEEEEECCCCCHHHHHHHHHHEEEECCCCCCCCCEEEECCCCEEEEEEEHHHHHHHHHHH
RALVRARRYDGQWRWIEMSATPRYSPDGHFIGLTGSSQDVTERLEIELAREQLLKSERAA
HHHHHHHHCCCCEEEEEECCCCCCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHH
RNEAENMARLKDEFLSTLSHELRTPLTTILGWSELLLQRIEEQHPCYKGLSVIASSAKVQ
HHHHHHHHHHHHHHHHHHHHHHHCHHHHHHCHHHHHHHHHHHHCCHHHHHHHHHHHHHHH
KRLISDMLDLSSMLLGKMQLEMEVLDLVEQIREVIGLYEQVVESKGQRLLLRAPEMPCLV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCEEE
LGDATRLRQIFENLLSNAIKFTPTDGCIDVEIDADGDSFRVSVTDSGDGIAAEFLPHLFS
ECCHHHHHHHHHHHHHCCEEECCCCCEEEEEEECCCCEEEEEEECCCCCHHHHHHHHHHH
RFRQADGTTTRCHGGLGLGLAIVQSLVEMHGGYVGATSPGLGKGAMFTVSLLRYVCAINV
HHHHCCCCCEEECCCCCHHHHHHHHHHHHCCCEEECCCCCCCCCHHHHHHHHHHHHHCCH
ADTVICDRRFGSVGDQGEY
HHHHHCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 3142862; 9387221; 9384377 [H]