Click here to switch to the map view.

The map label for this gene is wbnF [C]

Identifier: 28199200

GI number: 28199200

Start: 1541491

End: 1542462

Strand: Reverse

Name: wbnF [C]

Synonym: PD1314

Alternate gene names: 28199200

Gene position: 1542462-1541491 (Counterclockwise)

Preceding gene: 28199201

Following gene: 28199199

Centisome position: 61.21

GC content: 54.63

Gene sequence:

>972_bases
ATGACGGTGCTGGTCACAGGTGCGGCCGGCTTTATCGGCGCTCATGTGTGTCGCGCACTGGCCGCGCGTAACGATACCGT
GGTTGGGTTGGACAACTACAATGCCTACTACGATCCGCAACTGAAGCGCGACCGCGTGACTGCGCTGTGTCCGGATGTTT
ACATTCGAACGCTTGACTTGACTGACCGTGAGAGATGCGCGGCTCTTTTTAATGAGGTCCAGCCTGATCAGGTGGTGCAT
CTGGCGGCGCAGGCGGGGGTACGCTACTCCATTGAAAACCCACATGCCTACGTAGATAGCAATCTTGTCGGTTTTCTCAA
TGTGTTGGAGCTGTGTCGTCATTGCCGTGTGCAGCACCTGGTGTACGCTTCGAGCAGTTCAGTGTATGGGGACTCAGCGA
TTCCGCCGTTCTCGGAGCAGCAGCGCGTGGACCAGCCACGCTCTCTGTATGGGGCAACGAAGGTTGCTAACGAAGTGATG
GCTTATAGCTATGCTCAGCTCTATGGATTGCGTGCGACTGGCCTGCGTTTCTTTACTGTCTATGGTCCCTGGGGACGGCC
TGACATGGCGCCGCTGATCTTTAGCCGCGCTGTAATGGAAGGGCGACCTATTGAAGTATTCAACCATGGCCGCATGGAGC
GTGACTTCACCTTTATCAATGATATCGTGGCTGGTGTCATTGGTGCCCTTGATGCTCCGGGTGAACAAGCGGTGCCGCAT
CGTTTATTCAACTTAGGTAACCATACCCCCGTGTCGCTTGAGCATTTCATCAAGGTGATTGAACAGGCTGCTGGCCGTCC
TGCCGATAAGCATTACAAACCAATGCAACCGGGTGACATGATGGCCACAATGGCTGATATTGCAGCGGCACGCGCTGCAT
TTGGATTTGAACCAGTCACATCCATTGAGATCGGTATGCCGCAAGTGGTGGAGTGGTGCCGTCACTACTTTGATACCCGT
GCCTCAACGTAA

Upstream 100 bases:

>100_bases
AGCACGTTGCAGACTCTGTCATGCCTATAGATAGGATGGATGCATCTGCTACGACTGGTTGTATGGATGTGCGGGTCTGT
GGTTTCTGTGGAGGCTCCCG

Downstream 100 bases:

>100_bases
TGCTTTTTGATCGTAATAGTTGTTGCGATGGATAACCCATGACTGATTTGCAGCTTTCAGTTGTTGTTCCTGTGTTCAAT
GAGCGTGATAATGTCGCCCC

Product: nucleotide sugar epimerase

Products: UDPglucoseal [C]

Alternate protein names: ORF2 [H]

Number of amino acids: Translated: 323; Mature: 322

Protein sequence:

>323_residues
MTVLVTGAAGFIGAHVCRALAARNDTVVGLDNYNAYYDPQLKRDRVTALCPDVYIRTLDLTDRERCAALFNEVQPDQVVH
LAAQAGVRYSIENPHAYVDSNLVGFLNVLELCRHCRVQHLVYASSSSVYGDSAIPPFSEQQRVDQPRSLYGATKVANEVM
AYSYAQLYGLRATGLRFFTVYGPWGRPDMAPLIFSRAVMEGRPIEVFNHGRMERDFTFINDIVAGVIGALDAPGEQAVPH
RLFNLGNHTPVSLEHFIKVIEQAAGRPADKHYKPMQPGDMMATMADIAAARAAFGFEPVTSIEIGMPQVVEWCRHYFDTR
AST

Sequences:

>Translated_323_residues
MTVLVTGAAGFIGAHVCRALAARNDTVVGLDNYNAYYDPQLKRDRVTALCPDVYIRTLDLTDRERCAALFNEVQPDQVVH
LAAQAGVRYSIENPHAYVDSNLVGFLNVLELCRHCRVQHLVYASSSSVYGDSAIPPFSEQQRVDQPRSLYGATKVANEVM
AYSYAQLYGLRATGLRFFTVYGPWGRPDMAPLIFSRAVMEGRPIEVFNHGRMERDFTFINDIVAGVIGALDAPGEQAVPH
RLFNLGNHTPVSLEHFIKVIEQAAGRPADKHYKPMQPGDMMATMADIAAARAAFGFEPVTSIEIGMPQVVEWCRHYFDTR
AST
>Mature_322_residues
TVLVTGAAGFIGAHVCRALAARNDTVVGLDNYNAYYDPQLKRDRVTALCPDVYIRTLDLTDRERCAALFNEVQPDQVVHL
AAQAGVRYSIENPHAYVDSNLVGFLNVLELCRHCRVQHLVYASSSSVYGDSAIPPFSEQQRVDQPRSLYGATKVANEVMA
YSYAQLYGLRATGLRFFTVYGPWGRPDMAPLIFSRAVMEGRPIEVFNHGRMERDFTFINDIVAGVIGALDAPGEQAVPHR
LFNLGNHTPVSLEHFIKVIEQAAGRPADKHYKPMQPGDMMATMADIAAARAAFGFEPVTSIEIGMPQVVEWCRHYFDTRA
ST

Specific function: Galactose metabolism; third step. [C]

COG id: COG0451

COG function: function code MG; Nucleoside-diphosphate-sugar epimerases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sugar epimerase family. dTDP-glucose dehydratase subfamily [H]

Homologues:

Organism=Homo sapiens, GI42516563, Length=327, Percent_Identity=26.2996941896024, Blast_Score=117, Evalue=1e-26,
Organism=Homo sapiens, GI56237023, Length=337, Percent_Identity=27.299703264095, Blast_Score=100, Evalue=1e-21,
Organism=Homo sapiens, GI56118217, Length=337, Percent_Identity=27.299703264095, Blast_Score=100, Evalue=1e-21,
Organism=Homo sapiens, GI189083684, Length=337, Percent_Identity=27.299703264095, Blast_Score=100, Evalue=1e-21,
Organism=Homo sapiens, GI7657641, Length=327, Percent_Identity=24.7706422018349, Blast_Score=94, Evalue=1e-19,
Organism=Escherichia coli, GI48994969, Length=339, Percent_Identity=26.8436578171091, Blast_Score=104, Evalue=8e-24,
Organism=Escherichia coli, GI1786974, Length=335, Percent_Identity=25.3731343283582, Blast_Score=103, Evalue=2e-23,
Organism=Escherichia coli, GI1788353, Length=344, Percent_Identity=25.8720930232558, Blast_Score=100, Evalue=2e-22,
Organism=Caenorhabditis elegans, GI71982035, Length=337, Percent_Identity=26.7062314540059, Blast_Score=111, Evalue=4e-25,
Organism=Caenorhabditis elegans, GI71982038, Length=339, Percent_Identity=26.2536873156342, Blast_Score=108, Evalue=4e-24,
Organism=Caenorhabditis elegans, GI115532424, Length=325, Percent_Identity=25.8461538461538, Blast_Score=94, Evalue=9e-20,
Organism=Caenorhabditis elegans, GI17539532, Length=323, Percent_Identity=24.4582043343653, Blast_Score=94, Evalue=1e-19,
Organism=Caenorhabditis elegans, GI17568069, Length=326, Percent_Identity=23.3128834355828, Blast_Score=74, Evalue=8e-14,
Organism=Saccharomyces cerevisiae, GI6319493, Length=337, Percent_Identity=26.1127596439169, Blast_Score=84, Evalue=4e-17,
Organism=Drosophila melanogaster, GI21356223, Length=320, Percent_Identity=27.8125, Blast_Score=115, Evalue=4e-26,
Organism=Drosophila melanogaster, GI19923002, Length=335, Percent_Identity=28.0597014925373, Blast_Score=100, Evalue=1e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001509
- InterPro:   IPR016040
- InterPro:   IPR008089 [H]

Pfam domain/function: PF01370 Epimerase [H]

EC number: 5.1.3.2 [C]

Molecular weight: Translated: 35830; Mature: 35699

Theoretical pI: Translated: 6.58; Mature: 6.58

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTVLVTGAAGFIGAHVCRALAARNDTVVGLDNYNAYYDPQLKRDRVTALCPDVYIRTLDL
CEEEEECCHHHHHHHHHHHHHHCCCCEEEECCCCCEECCCCCCCHHHHCCCCHHEEEECC
TDRERCAALFNEVQPDQVVHLAAQAGVRYSIENPHAYVDSNLVGFLNVLELCRHCRVQHL
CCHHHHHHHHHCCCHHHHHHHHHHCCCEEEECCCCHHHCCHHHHHHHHHHHHHHHHHHHH
VYASSSSVYGDSAIPPFSEQQRVDQPRSLYGATKVANEVMAYSYAQLYGLRATGLRFFTV
EEECCCCCCCCCCCCCCCHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEE
YGPWGRPDMAPLIFSRAVMEGRPIEVFNHGRMERDFTFINDIVAGVIGALDAPGEQAVPH
ECCCCCCCHHHHHHHHHHHCCCCEEECCCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHH
RLFNLGNHTPVSLEHFIKVIEQAAGRPADKHYKPMQPGDMMATMADIAAARAAFGFEPVT
HHHHCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCC
SIEIGMPQVVEWCRHYFDTRAST
EEECCCHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
TVLVTGAAGFIGAHVCRALAARNDTVVGLDNYNAYYDPQLKRDRVTALCPDVYIRTLDL
EEEEECCHHHHHHHHHHHHHHCCCCEEEECCCCCEECCCCCCCHHHHCCCCHHEEEECC
TDRERCAALFNEVQPDQVVHLAAQAGVRYSIENPHAYVDSNLVGFLNVLELCRHCRVQHL
CCHHHHHHHHHCCCHHHHHHHHHHCCCEEEECCCCHHHCCHHHHHHHHHHHHHHHHHHHH
VYASSSSVYGDSAIPPFSEQQRVDQPRSLYGATKVANEVMAYSYAQLYGLRATGLRFFTV
EEECCCCCCCCCCCCCCCHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEE
YGPWGRPDMAPLIFSRAVMEGRPIEVFNHGRMERDFTFINDIVAGVIGALDAPGEQAVPH
ECCCCCCCHHHHHHHHHHHCCCCEEECCCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHH
RLFNLGNHTPVSLEHFIKVIEQAAGRPADKHYKPMQPGDMMATMADIAAARAAFGFEPVT
HHHHCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCC
SIEIGMPQVVEWCRHYFDTRAST
EEECCCHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NAD+ [C]

Metal ions: NA

Kcat value (1/min): 57600 [C]

Specific activity: 233.3

Km value (mM): 0.256 {UDPgalactose}} 0.225 {UDPgalactose}} 0.2 {UDPgalactose}} 0.18 {UDPgalactose}} 0.16 {UDPgalactose}} 0.14 {UDPgalactose}} 0.048 {UDPgalactose}} 0.026 {UDPgalactose}} [C]

Substrates: UDPglucose [C]

Specific reaction: UDPglucose <==> UDPglucoseal [C]

General reaction: Epimerization (specificity for carbon forming a hexosulose) [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7682279 [H]