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The map label for this gene is thrA [H]

Identifier: 28199159

GI number: 28199159

Start: 1483149

End: 1485635

Strand: Reverse

Name: thrA [H]

Synonym: PD1273

Alternate gene names: 28199159

Gene position: 1485635-1483149 (Counterclockwise)

Preceding gene: 28199160

Following gene: 28199158

Centisome position: 58.96

GC content: 56.74

Gene sequence:

>2487_bases
TTGTCCACCGTTGAAACTCCTGTCTACGTTCCGCGTACCGTCGTTCACAAATTCGGTGGGACTTCGGTTGCCGATGCGGA
TTGCTATCGCCATGTCACGCGTCTCCTATTAGCACGCGAAGAGACTGTGCAGGTCACCATCGTTTCTGCGATGAAAGGGG
TGACTGATACGTTGATTGCACTGGCCGAACAGGCAGCCAATCATTCTCCCGACTGGCAGGTGTTGTGGCAGCAAACCCGC
ACGCGTCATTGCGACGCCGCTCAGGCATTGCTTGGAGAGCATGCCGCTACGCTGCAAGATTGGTTGAATACCCGTTTTAA
TGAACTGCATGAAATGCTGGTGGCCTTGTCCGTCATCGGGATGCTTCCATCTGAAGTGCTGGACCGCTTACAAGGCTTGG
GCGAGGTCTACTCCGCACAGTTGCTTGGGCATTATTTGCAATCCCAGGGTGAGGATTGTGTCGTTCTGGATGCCCGCCAA
GTCCTTGTGGTGAGTCCTGGTGAGTTTGGCGCCGAAGTCGATTGGGCAACCAGTGCCCAGCACTTGGCCCATTGGCGTAT
GCAGCATCCACAGCGGCGTGTGGTGGTCACCGGTTTCGTTGCCTCTGATCGTACTGGACGCGCCATCACCCTGGGTCGCA
ACGGCAGTGATTATTCCGGTGCCATCTTCGCGGCGTTGTTTGAGGCCGATGAGCTTCACATCTGGACAGATGTTGATGGA
GTCATGTCCGCCGACCCCCGGGTCGTCCCGGATGCGGTGCAACTGGATGCCTTGAGTTACGACGAAGCATGTGAATTGGC
CTATTTCGGCGCCAAGGTAGTGCATCCACAAACCATGTCGCCGGTGATGAAACGCGGAGTGCCGATCATCATTCGAAACA
CCTTCCAACCCGGACATCCAGGGACTCGGATCACCGCTGATAGCGTGGTGAGTGGATCTGTTAAAGGCCTGACCCTGAGT
CCAGGACTGGCCGTGCTGAACCTGGAAGGCACGGGGCTGATCGGAGTCCCTGGTACCGCTGAACGCGTGTTTGCCGCCCT
GCGTAATGCACGTTTGTCTGTCGTGATGATCTCACAGGGATCTTCGGAGCATTCCATTTGCTGCCTCGTGCACCAAACCG
AGGCCGAGCGTGCACGTGATGCGTTGTTGTATGCCTTTGCCCATGAATTGGCCATAGGGCATGTCCAGCGCGTACAACTG
ACCAACAATATCAGCGTATTGGCGGCGGTTGGCGACGGCATGGCTGGTCACCTGGGTGTAGCGGCGCGCTTGTTTGAATC
CTTAAGGCGCGCCCATGTCAACATCCTGGCCATTGCCCAGGGGTCTTCAGAGCGCAATATTTCGGTGGCAATCGACAGTC
AGCAGGCGACGAAGGCACTGCGCGCTGCACACGCTGCTTTCTGGTTATCACCCCAGACATTTTCGATTGGTGTGATTGGT
CCGGGTCACGTTGGTGAGGCCCTGTTGGACCAGCTATGTGCTGCACAGCAGCAATTACTGTCCAAGGCAAATCTGGATCT
GCGTTTGCGTGCGATCGTTTCACGGCGGCGTATGTTATTGGATGAGCGTGGCCTGGGGAGTGACTGGCGCCACGCCTTCG
CCGCCGCACCAGAGGCGGTCGATCTGGATGCCTTTACCGCACACTTACAGGCCGCCCATCTGCCTCATGCAGTCATCATT
GACTGTAGCGGCAGTGCTGAGGTGGCTGATCGCTATGCCGACTGGCTGGCAGCCGGTATCCATGTCGTGACACCGAATAA
ACAGGCTGGCTCCGGCCCCCTGCATCGCTACCACGCGATTCGTGCAGCAGCAGATGCCAGCGGCGCACGCTTTCGCTATG
AAGCCACAGTGGGCGCAGGCTTACCGGTGATTACGACATTGCGTGACTTGATGGATACCGGTGATAGCGTCACCTCAATC
GAAGGGATCTTTTCCGGAACTCTGGCGTGGCTATTCAATAAATATGATGGCAGCGTCCCATTCTCCCAATTAATCGCCCA
GGCGCGAGCGATGGGATATACCGAGCCGGATCCACGCGATGACCTGTCTGGGGTTGACGTGGCGCGTAAGCTGGTGATTC
TGGCGCGCGATGCGGGGCGTGATATCAGCCTAGAAGATGTTGCGGTAGAAAGCCTGGTGCCGGAGACATTGTGTCAACTC
AGCGTTGCCGATTTCATGGAACGACTCAATGAAATTGATGCCACCTTTGCAGAGCGTTTGGCTGCGGCCAAACAGCGCGG
TTGCGTATTGCGTTACGTCGCACAACTGTCCCCTGGACACGTCCCTAGTGTCAGCTTGATTGAATTGCCTTCTGACCATG
CCTTCGTCAATCTTCGTTTGACCGATAACGTAGTGCAATTTACAACTCGTCGTTATTCCGAAAACCCCTTGATCGTACAG
GGCCCTGGCGCCGGTCCAGAGGTCACCGCAGCCGGTGTTTTTGCGGATTTACTGCGAGTTGCAGCGGGCGAGGGCGCACG
CTTGTGA

Upstream 100 bases:

>100_bases
AGGCCCCGCACCAGAACTCCAGGTTGCGGGTCAAACCCTCAGCCTGATGAGAATGGGGCCTCCATCATGAGGTCTGTTGA
TGTCATCGTCTGCTGTCCGG

Downstream 100 bases:

>100_bases
ATCGGACCTTGCAACCAGACAGCGGCGCATCTCAAACAGCGCCCACAGCGCATCAGGCACGCGCCTTCGCACCGGCATCA
GTGGCGAACGTTGCCGTTGG

Product: bifunctional aspartokinase I/homeserine dehydrogenase I

Products: NA

Alternate protein names: Aspartokinase I/homoserine dehydrogenase I; AKI-HDI; Aspartokinase; Homoserine dehydrogenase [H]

Number of amino acids: Translated: 828; Mature: 827

Protein sequence:

>828_residues
MSTVETPVYVPRTVVHKFGGTSVADADCYRHVTRLLLAREETVQVTIVSAMKGVTDTLIALAEQAANHSPDWQVLWQQTR
TRHCDAAQALLGEHAATLQDWLNTRFNELHEMLVALSVIGMLPSEVLDRLQGLGEVYSAQLLGHYLQSQGEDCVVLDARQ
VLVVSPGEFGAEVDWATSAQHLAHWRMQHPQRRVVVTGFVASDRTGRAITLGRNGSDYSGAIFAALFEADELHIWTDVDG
VMSADPRVVPDAVQLDALSYDEACELAYFGAKVVHPQTMSPVMKRGVPIIIRNTFQPGHPGTRITADSVVSGSVKGLTLS
PGLAVLNLEGTGLIGVPGTAERVFAALRNARLSVVMISQGSSEHSICCLVHQTEAERARDALLYAFAHELAIGHVQRVQL
TNNISVLAAVGDGMAGHLGVAARLFESLRRAHVNILAIAQGSSERNISVAIDSQQATKALRAAHAAFWLSPQTFSIGVIG
PGHVGEALLDQLCAAQQQLLSKANLDLRLRAIVSRRRMLLDERGLGSDWRHAFAAAPEAVDLDAFTAHLQAAHLPHAVII
DCSGSAEVADRYADWLAAGIHVVTPNKQAGSGPLHRYHAIRAAADASGARFRYEATVGAGLPVITTLRDLMDTGDSVTSI
EGIFSGTLAWLFNKYDGSVPFSQLIAQARAMGYTEPDPRDDLSGVDVARKLVILARDAGRDISLEDVAVESLVPETLCQL
SVADFMERLNEIDATFAERLAAAKQRGCVLRYVAQLSPGHVPSVSLIELPSDHAFVNLRLTDNVVQFTTRRYSENPLIVQ
GPGAGPEVTAAGVFADLLRVAAGEGARL

Sequences:

>Translated_828_residues
MSTVETPVYVPRTVVHKFGGTSVADADCYRHVTRLLLAREETVQVTIVSAMKGVTDTLIALAEQAANHSPDWQVLWQQTR
TRHCDAAQALLGEHAATLQDWLNTRFNELHEMLVALSVIGMLPSEVLDRLQGLGEVYSAQLLGHYLQSQGEDCVVLDARQ
VLVVSPGEFGAEVDWATSAQHLAHWRMQHPQRRVVVTGFVASDRTGRAITLGRNGSDYSGAIFAALFEADELHIWTDVDG
VMSADPRVVPDAVQLDALSYDEACELAYFGAKVVHPQTMSPVMKRGVPIIIRNTFQPGHPGTRITADSVVSGSVKGLTLS
PGLAVLNLEGTGLIGVPGTAERVFAALRNARLSVVMISQGSSEHSICCLVHQTEAERARDALLYAFAHELAIGHVQRVQL
TNNISVLAAVGDGMAGHLGVAARLFESLRRAHVNILAIAQGSSERNISVAIDSQQATKALRAAHAAFWLSPQTFSIGVIG
PGHVGEALLDQLCAAQQQLLSKANLDLRLRAIVSRRRMLLDERGLGSDWRHAFAAAPEAVDLDAFTAHLQAAHLPHAVII
DCSGSAEVADRYADWLAAGIHVVTPNKQAGSGPLHRYHAIRAAADASGARFRYEATVGAGLPVITTLRDLMDTGDSVTSI
EGIFSGTLAWLFNKYDGSVPFSQLIAQARAMGYTEPDPRDDLSGVDVARKLVILARDAGRDISLEDVAVESLVPETLCQL
SVADFMERLNEIDATFAERLAAAKQRGCVLRYVAQLSPGHVPSVSLIELPSDHAFVNLRLTDNVVQFTTRRYSENPLIVQ
GPGAGPEVTAAGVFADLLRVAAGEGARL
>Mature_827_residues
STVETPVYVPRTVVHKFGGTSVADADCYRHVTRLLLAREETVQVTIVSAMKGVTDTLIALAEQAANHSPDWQVLWQQTRT
RHCDAAQALLGEHAATLQDWLNTRFNELHEMLVALSVIGMLPSEVLDRLQGLGEVYSAQLLGHYLQSQGEDCVVLDARQV
LVVSPGEFGAEVDWATSAQHLAHWRMQHPQRRVVVTGFVASDRTGRAITLGRNGSDYSGAIFAALFEADELHIWTDVDGV
MSADPRVVPDAVQLDALSYDEACELAYFGAKVVHPQTMSPVMKRGVPIIIRNTFQPGHPGTRITADSVVSGSVKGLTLSP
GLAVLNLEGTGLIGVPGTAERVFAALRNARLSVVMISQGSSEHSICCLVHQTEAERARDALLYAFAHELAIGHVQRVQLT
NNISVLAAVGDGMAGHLGVAARLFESLRRAHVNILAIAQGSSERNISVAIDSQQATKALRAAHAAFWLSPQTFSIGVIGP
GHVGEALLDQLCAAQQQLLSKANLDLRLRAIVSRRRMLLDERGLGSDWRHAFAAAPEAVDLDAFTAHLQAAHLPHAVIID
CSGSAEVADRYADWLAAGIHVVTPNKQAGSGPLHRYHAIRAAADASGARFRYEATVGAGLPVITTLRDLMDTGDSVTSIE
GIFSGTLAWLFNKYDGSVPFSQLIAQARAMGYTEPDPRDDLSGVDVARKLVILARDAGRDISLEDVAVESLVPETLCQLS
VADFMERLNEIDATFAERLAAAKQRGCVLRYVAQLSPGHVPSVSLIELPSDHAFVNLRLTDNVVQFTTRRYSENPLIVQG
PGAGPEVTAAGVFADLLRVAAGEGARL

Specific function: Catalyzes 2 nonconsecutive reactions in the common biosynthetic pathway leading from Asp to diaminopimelate and Lys, to Met, and to Thr and Ile. [C]

COG id: COG0527

COG function: function code E; Aspartokinases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 ACT domains [H]

Homologues:

Organism=Escherichia coli, GI1786183, Length=818, Percent_Identity=40.2200488997555, Blast_Score=564, Evalue=1e-162,
Organism=Escherichia coli, GI1790376, Length=820, Percent_Identity=34.1463414634146, Blast_Score=404, Evalue=1e-113,
Organism=Escherichia coli, GI1790455, Length=464, Percent_Identity=29.0948275862069, Blast_Score=149, Evalue=1e-36,
Organism=Saccharomyces cerevisiae, GI6322599, Length=367, Percent_Identity=38.6920980926431, Blast_Score=221, Evalue=3e-58,
Organism=Saccharomyces cerevisiae, GI6320893, Length=502, Percent_Identity=27.4900398406374, Blast_Score=157, Evalue=6e-39,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002912
- InterPro:   IPR001048
- InterPro:   IPR005106
- InterPro:   IPR001341
- InterPro:   IPR018042
- InterPro:   IPR011147
- InterPro:   IPR001342
- InterPro:   IPR019811
- InterPro:   IPR016040 [H]

Pfam domain/function: PF00696 AA_kinase; PF01842 ACT; PF00742 Homoserine_dh; PF03447 NAD_binding_3 [H]

EC number: =2.7.2.4; =1.1.1.3 [H]

Molecular weight: Translated: 89057; Mature: 88926

Theoretical pI: Translated: 6.11; Mature: 6.11

Prosite motif: PS01042 HOMOSER_DHGENASE ; PS00324 ASPARTOKINASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTVETPVYVPRTVVHKFGGTSVADADCYRHVTRLLLAREETVQVTIVSAMKGVTDTLIA
CCCCCCCCCCCHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCEEEEEHHHHCCHHHHHHH
LAEQAANHSPDWQVLWQQTRTRHCDAAQALLGEHAATLQDWLNTRFNELHEMLVALSVIG
HHHHHCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MLPSEVLDRLQGLGEVYSAQLLGHYLQSQGEDCVVLDARQVLVVSPGEFGAEVDWATSAQ
CCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCEEEEECCCCCCCCCCCCHHHH
HLAHWRMQHPQRRVVVTGFVASDRTGRAITLGRNGSDYSGAIFAALFEADELHIWTDVDG
HHHHHHHCCCCCEEEEEEEEECCCCCCEEEECCCCCCCCCHHHHHEECCCCEEEEECCCC
VMSADPRVVPDAVQLDALSYDEACELAYFGAKVVHPQTMSPVMKRGVPIIIRNTFQPGHP
HHCCCCCCCCCHHHCCCCCCCHHHHHHHHCCEEECCCHHHHHHHCCCCEEEECCCCCCCC
GTRITADSVVSGSVKGLTLSPGLAVLNLEGTGLIGVPGTAERVFAALRNARLSVVMISQG
CCEEEHHHHHCCCCCCEEECCCEEEEEECCCEEEECCCHHHHHHHHHHCCCEEEEEEECC
SSEHSICCLVHQTEAERARDALLYAFAHELAIGHVQRVQLTNNISVLAAVGDGMAGHLGV
CCCCCEEEEEECCHHHHHHHHHHHHHHHHHHHCCCEEEEEECCEEEEEEECCCCCHHHHH
AARLFESLRRAHVNILAIAQGSSERNISVAIDSQQATKALRAAHAAFWLSPQTFSIGVIG
HHHHHHHHHHCCEEEEEEECCCCCCEEEEEECCHHHHHHHHHHHHHEEECCCEEEEEECC
PGHVGEALLDQLCAAQQQLLSKANLDLRLRAIVSRRRMLLDERGLGSDWRHAFAAAPEAV
CCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCC
DLDAFTAHLQAAHLPHAVIIDCSGSAEVADRYADWLAAGIHVVTPNKQAGSGPLHRYHAI
CHHHHHHHHHHHCCCEEEEEECCCCHHHHHHHHHHHHCCEEEECCCCCCCCCCHHHHHHH
RAAADASGARFRYEATVGAGLPVITTLRDLMDTGDSVTSIEGIFSGTLAWLFNKYDGSVP
HHHCCCCCCEEEEEEECCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCC
FSQLIAQARAMGYTEPDPRDDLSGVDVARKLVILARDAGRDISLEDVAVESLVPETLCQL
HHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHEEEEECCCCCCCHHHHHHHHHHHHHHHHH
SVADFMERLNEIDATFAERLAAAKQRGCVLRYVAQLSPGHVPSVSLIELPSDHAFVNLRL
HHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCCCCEEEEECCCCCEEEEEEE
TDNVVQFTTRRYSENPLIVQGPGAGPEVTAAGVFADLLRVAAGEGARL
ECHHHHHHHHHCCCCCEEEECCCCCCCCHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
STVETPVYVPRTVVHKFGGTSVADADCYRHVTRLLLAREETVQVTIVSAMKGVTDTLIA
CCCCCCCCCCHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCEEEEEHHHHCCHHHHHHH
LAEQAANHSPDWQVLWQQTRTRHCDAAQALLGEHAATLQDWLNTRFNELHEMLVALSVIG
HHHHHCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MLPSEVLDRLQGLGEVYSAQLLGHYLQSQGEDCVVLDARQVLVVSPGEFGAEVDWATSAQ
CCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCEEEEECCCCCCCCCCCCHHHH
HLAHWRMQHPQRRVVVTGFVASDRTGRAITLGRNGSDYSGAIFAALFEADELHIWTDVDG
HHHHHHHCCCCCEEEEEEEEECCCCCCEEEECCCCCCCCCHHHHHEECCCCEEEEECCCC
VMSADPRVVPDAVQLDALSYDEACELAYFGAKVVHPQTMSPVMKRGVPIIIRNTFQPGHP
HHCCCCCCCCCHHHCCCCCCCHHHHHHHHCCEEECCCHHHHHHHCCCCEEEECCCCCCCC
GTRITADSVVSGSVKGLTLSPGLAVLNLEGTGLIGVPGTAERVFAALRNARLSVVMISQG
CCEEEHHHHHCCCCCCEEECCCEEEEEECCCEEEECCCHHHHHHHHHHCCCEEEEEEECC
SSEHSICCLVHQTEAERARDALLYAFAHELAIGHVQRVQLTNNISVLAAVGDGMAGHLGV
CCCCCEEEEEECCHHHHHHHHHHHHHHHHHHHCCCEEEEEECCEEEEEEECCCCCHHHHH
AARLFESLRRAHVNILAIAQGSSERNISVAIDSQQATKALRAAHAAFWLSPQTFSIGVIG
HHHHHHHHHHCCEEEEEEECCCCCCEEEEEECCHHHHHHHHHHHHHEEECCCEEEEEECC
PGHVGEALLDQLCAAQQQLLSKANLDLRLRAIVSRRRMLLDERGLGSDWRHAFAAAPEAV
CCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCC
DLDAFTAHLQAAHLPHAVIIDCSGSAEVADRYADWLAAGIHVVTPNKQAGSGPLHRYHAI
CHHHHHHHHHHHCCCEEEEEECCCCHHHHHHHHHHHHCCEEEECCCCCCCCCCHHHHHHH
RAAADASGARFRYEATVGAGLPVITTLRDLMDTGDSVTSIEGIFSGTLAWLFNKYDGSVP
HHHCCCCCCEEEEEEECCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCC
FSQLIAQARAMGYTEPDPRDDLSGVDVARKLVILARDAGRDISLEDVAVESLVPETLCQL
HHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHEEEEECCCCCCCHHHHHHHHHHHHHHHHH
SVADFMERLNEIDATFAERLAAAKQRGCVLRYVAQLSPGHVPSVSLIELPSDHAFVNLRL
HHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCCCCEEEEECCCCCEEEEEEE
TDNVVQFTTRRYSENPLIVQGPGAGPEVTAAGVFADLLRVAAGEGARL
ECHHHHHHHHHCCCCCEEEECCCCCCCCHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7003595; 1630901; 7610040; 9278503; 6277952; 2410621; 387092; 6298218; 390305 [H]