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The map label for this gene is hisS

Identifier: 28199156

GI number: 28199156

Start: 1479126

End: 1480526

Strand: Reverse

Name: hisS

Synonym: PD1270

Alternate gene names: 28199156

Gene position: 1480526-1479126 (Counterclockwise)

Preceding gene: 28199157

Following gene: 28199155

Centisome position: 58.76

GC content: 55.25

Gene sequence:

>1401_bases
GTGATCAAACCTCGCACGCCGCCTGGCGTCTTGGAACTGCTACCTCGCGAACAGATTGCCTTCCAGCGCATGCTAGATGT
CATCCGCCGCAACTATGAGCGTTTCGGGTTTTTACCAGTAGAAACCCCGGTGTTTGAACTGTCTGATGTCTTATTGACCA
AATCTGGTGGTGAGACAGAGCGTCAAGTCTATTTCGTGCAATCCACCGGCACCCTAGCCAATGCCGCTGAATCTGGAGCG
ACCCGCCTACCGGAACTGGCATTGCGCTTTGATTTGACCGTGCCCCTGGCGCGCTATGTCGCCGAATATGAGCACGTCTT
GGCCTTTCCGTTCCGACGCTACCAAATCCAGCGCGTCTATCGCGGCGAGCGCGCCCAGCGTGGCCGCTTCCGTGAATTTT
ATCAATGCGATATTGATGTCATTGGCAAACAGACCCTGAGCATTCGCTACGATGCCGAGGTCCTGGCGGTCATCCATGCC
GTGTTTTCTGAGTTGGGGATCGGAGATTTCCAAGTCCAGTTAAACAACCGCAAAGTATTGCGGGGCTTCCTTGAGAGCCA
GGGAGTGCGTGACGGCGAATTGCAATTGGCTGTATTGCGCGAGGTGGATAAGCTCGATAAACGCGGTGTCCTTGACGTGA
GAGATACATTGATAGGGCAGGGCTTCGGTATTCCTGCCGCGCAGGTCGAAAATATCCTGACTTTTGTTGCCACCCGTTCC
ACCAGCCATGCCGATGCATTGGCCCGTCTGGATGCACTGATCCAAGACTCAGGGCCAGAGGCACACGACATGCTGCGCCA
AGGTGTTGCCGAATTGCGTGAAGTGCTGACTCTGGTCAACGTCCTGGGTGTCCCCGAACACGCCTACCGCCTGAACTTCT
CCATTGCCCGCGGTTTGGATTACTACACCGGCACGGTGTACGAAACCGCGCTGATCAATCACCCCCAGATTGGTTCCATC
TGCTCCGGGGGGCGCTACGAAAACCTCGCCAATCATTACACCCAATCGAAATTGCCTGGTGTGGGCATCTCGATTGGTTT
AACCCGCCTGTTCTGGCAACTGCGTGATGCTGGTTTGATAGATGGCATTGCAGAAAGCAGCGTCCAAGCCATGGTGGTAT
TGATGGATGAAGCCACACTGGACGATGCCTTGGACATTGCCCGTCGCCTGCGCATCGGCGGAATCAATACCGAAGTACAG
ATGGAAGCAAAAAAGCTCAGCAAACAATTCCAATATGCCTCCCGTGCGGGGATCCGTTTCGTCGTCCTGGCCGGTGACGA
TGAGCGCGCGCGTGGCGTGGTTGCCGTCAAAGACCTGACCCGTGAACAGCAATTTGAGATCCCACGAGAAGAGTTAGCGA
GCACCCTGCAAGTCGAACTGGAGCAAGCCAAGGTTATGTGA

Upstream 100 bases:

>100_bases
GCGATATTTCGAGTTTTCCCGCAGATGCCATGCCACCGGAAGCGATTGCAGCCGGTAGAATGGCGCCCTGCTTTTATCTG
TTTACTTTGGAGTTGCGTTT

Downstream 100 bases:

>100_bases
CCGCCTCCGCGCTGCGGCAAGCCCATCCCATGCCCCCCCCAGAGAACACCCGCCCCTCTTTGAGCCGCAGCAGGCCTGCC
ACCGAGTGCTGCTTCCACTG

Product: histidyl-tRNA synthetase

Products: NA

Alternate protein names: Histidine--tRNA ligase; HisRS

Number of amino acids: Translated: 466; Mature: 466

Protein sequence:

>466_residues
MIKPRTPPGVLELLPREQIAFQRMLDVIRRNYERFGFLPVETPVFELSDVLLTKSGGETERQVYFVQSTGTLANAAESGA
TRLPELALRFDLTVPLARYVAEYEHVLAFPFRRYQIQRVYRGERAQRGRFREFYQCDIDVIGKQTLSIRYDAEVLAVIHA
VFSELGIGDFQVQLNNRKVLRGFLESQGVRDGELQLAVLREVDKLDKRGVLDVRDTLIGQGFGIPAAQVENILTFVATRS
TSHADALARLDALIQDSGPEAHDMLRQGVAELREVLTLVNVLGVPEHAYRLNFSIARGLDYYTGTVYETALINHPQIGSI
CSGGRYENLANHYTQSKLPGVGISIGLTRLFWQLRDAGLIDGIAESSVQAMVVLMDEATLDDALDIARRLRIGGINTEVQ
MEAKKLSKQFQYASRAGIRFVVLAGDDERARGVVAVKDLTREQQFEIPREELASTLQVELEQAKVM

Sequences:

>Translated_466_residues
MIKPRTPPGVLELLPREQIAFQRMLDVIRRNYERFGFLPVETPVFELSDVLLTKSGGETERQVYFVQSTGTLANAAESGA
TRLPELALRFDLTVPLARYVAEYEHVLAFPFRRYQIQRVYRGERAQRGRFREFYQCDIDVIGKQTLSIRYDAEVLAVIHA
VFSELGIGDFQVQLNNRKVLRGFLESQGVRDGELQLAVLREVDKLDKRGVLDVRDTLIGQGFGIPAAQVENILTFVATRS
TSHADALARLDALIQDSGPEAHDMLRQGVAELREVLTLVNVLGVPEHAYRLNFSIARGLDYYTGTVYETALINHPQIGSI
CSGGRYENLANHYTQSKLPGVGISIGLTRLFWQLRDAGLIDGIAESSVQAMVVLMDEATLDDALDIARRLRIGGINTEVQ
MEAKKLSKQFQYASRAGIRFVVLAGDDERARGVVAVKDLTREQQFEIPREELASTLQVELEQAKVM
>Mature_466_residues
MIKPRTPPGVLELLPREQIAFQRMLDVIRRNYERFGFLPVETPVFELSDVLLTKSGGETERQVYFVQSTGTLANAAESGA
TRLPELALRFDLTVPLARYVAEYEHVLAFPFRRYQIQRVYRGERAQRGRFREFYQCDIDVIGKQTLSIRYDAEVLAVIHA
VFSELGIGDFQVQLNNRKVLRGFLESQGVRDGELQLAVLREVDKLDKRGVLDVRDTLIGQGFGIPAAQVENILTFVATRS
TSHADALARLDALIQDSGPEAHDMLRQGVAELREVLTLVNVLGVPEHAYRLNFSIARGLDYYTGTVYETALINHPQIGSI
CSGGRYENLANHYTQSKLPGVGISIGLTRLFWQLRDAGLIDGIAESSVQAMVVLMDEATLDDALDIARRLRIGGINTEVQ
MEAKKLSKQFQYASRAGIRFVVLAGDDERARGVVAVKDLTREQQFEIPREELASTLQVELEQAKVM

Specific function: Unknown

COG id: COG0124

COG function: function code J; Histidyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family

Homologues:

Organism=Homo sapiens, GI6996014, Length=468, Percent_Identity=31.4102564102564, Blast_Score=222, Evalue=6e-58,
Organism=Homo sapiens, GI15029520, Length=476, Percent_Identity=28.1512605042017, Blast_Score=202, Evalue=7e-52,
Organism=Homo sapiens, GI65287717, Length=455, Percent_Identity=23.7362637362637, Blast_Score=81, Evalue=2e-15,
Organism=Escherichia coli, GI1788861, Length=473, Percent_Identity=26.8498942917548, Blast_Score=110, Evalue=3e-25,
Organism=Caenorhabditis elegans, GI71993686, Length=462, Percent_Identity=32.2510822510823, Blast_Score=202, Evalue=2e-52,
Organism=Caenorhabditis elegans, GI71993693, Length=462, Percent_Identity=32.2510822510823, Blast_Score=202, Evalue=3e-52,
Organism=Saccharomyces cerevisiae, GI6325290, Length=477, Percent_Identity=28.7211740041929, Blast_Score=174, Evalue=3e-44,
Organism=Drosophila melanogaster, GI24643061, Length=481, Percent_Identity=29.7297297297297, Blast_Score=189, Evalue=5e-48,
Organism=Drosophila melanogaster, GI24643059, Length=499, Percent_Identity=28.6573146292585, Blast_Score=179, Evalue=5e-45,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): SYH_XYLF2 (B2I5Y4)

Other databases:

- EMBL:   CP001011
- RefSeq:   YP_001830047.1
- ProteinModelPortal:   B2I5Y4
- SMR:   B2I5Y4
- GeneID:   6202235
- GenomeReviews:   CP001011_GR
- KEGG:   xfn:XfasM23_1355
- HOGENOM:   HBG616575
- OMA:   ALDIARC
- ProtClustDB:   PRK00037
- GO:   GO:0005737
- HAMAP:   MF_00127
- InterPro:   IPR002314
- InterPro:   IPR006195
- InterPro:   IPR004154
- InterPro:   IPR015807
- InterPro:   IPR004516
- Gene3D:   G3DSA:3.40.50.800
- PANTHER:   PTHR11476
- PIRSF:   PIRSF001549
- TIGRFAMs:   TIGR00442

Pfam domain/function: PF03129 HGTP_anticodon; PF00587 tRNA-synt_2b; SSF52954 Anticodon_bd

EC number: =6.1.1.21

Molecular weight: Translated: 52240; Mature: 52240

Theoretical pI: Translated: 5.77; Mature: 5.77

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIKPRTPPGVLELLPREQIAFQRMLDVIRRNYERFGFLPVETPVFELSDVLLTKSGGETE
CCCCCCCCHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHEECCCCCCC
RQVYFVQSTGTLANAAESGATRLPELALRFDLTVPLARYVAEYEHVLAFPFRRYQIQRVY
EEEEEEECCCCHHHHHHCCCCCCCHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RGERAQRGRFREFYQCDIDVIGKQTLSIRYDAEVLAVIHAVFSELGIGDFQVQLNNRKVL
CCCHHHCCHHHHHHHCCHHEECCEEEEEEECHHHHHHHHHHHHHCCCCEEEEEECCHHHH
RGFLESQGVRDGELQLAVLREVDKLDKRGVLDVRDTLIGQGFGIPAAQVENILTFVATRS
HHHHHHCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHCCC
TSHADALARLDALIQDSGPEAHDMLRQGVAELREVLTLVNVLGVPEHAYRLNFSIARGLD
CCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHEEEEHHHHCCC
YYTGTVYETALINHPQIGSICSGGRYENLANHYTQSKLPGVGISIGLTRLFWQLRDAGLI
HHCCCHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCHH
DGIAESSVQAMVVLMDEATLDDALDIARRLRIGGINTEVQMEAKKLSKQFQYASRAGIRF
HHHHHHHHEEEEEEECCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCEE
VVLAGDDERARGVVAVKDLTREQQFEIPREELASTLQVELEQAKVM
EEEECCCCHHCCEEEEHHHHHHHHCCCCHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MIKPRTPPGVLELLPREQIAFQRMLDVIRRNYERFGFLPVETPVFELSDVLLTKSGGETE
CCCCCCCCHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHEECCCCCCC
RQVYFVQSTGTLANAAESGATRLPELALRFDLTVPLARYVAEYEHVLAFPFRRYQIQRVY
EEEEEEECCCCHHHHHHCCCCCCCHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RGERAQRGRFREFYQCDIDVIGKQTLSIRYDAEVLAVIHAVFSELGIGDFQVQLNNRKVL
CCCHHHCCHHHHHHHCCHHEECCEEEEEEECHHHHHHHHHHHHHCCCCEEEEEECCHHHH
RGFLESQGVRDGELQLAVLREVDKLDKRGVLDVRDTLIGQGFGIPAAQVENILTFVATRS
HHHHHHCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHCCC
TSHADALARLDALIQDSGPEAHDMLRQGVAELREVLTLVNVLGVPEHAYRLNFSIARGLD
CCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHEEEEHHHHCCC
YYTGTVYETALINHPQIGSICSGGRYENLANHYTQSKLPGVGISIGLTRLFWQLRDAGLI
HHCCCHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCHH
DGIAESSVQAMVVLMDEATLDDALDIARRLRIGGINTEVQMEAKKLSKQFQYASRAGIRF
HHHHHHHHEEEEEEECCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCEE
VVLAGDDERARGVVAVKDLTREQQFEIPREELASTLQVELEQAKVM
EEEECCCCHHCCEEEEHHHHHHHHCCCCHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA