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The map label for this gene is rnhA [H]

Identifier: 28199105

GI number: 28199105

Start: 1418930

End: 1419382

Strand: Reverse

Name: rnhA [H]

Synonym: PD1218

Alternate gene names: 28199105

Gene position: 1419382-1418930 (Counterclockwise)

Preceding gene: 28199106

Following gene: 28199104

Centisome position: 56.33

GC content: 52.1

Gene sequence:

>453_bases
ATGAAATTGATCAACGCGTACACGGACGGTTCTTGTCTTGGTAATCCTGGTCCTGGTGGTTGGGCTGTGTTGTTGCGTTA
TAAAAACAATGAGAAGGAGTTGGTTGGTGGTGAGCTTGATACGACCAATAATCGAATGGAGCTGATGGCTGCGATTATGG
CTCTGGAGCGGTTGAGTGAGCCTTGTCAGATCAAGCTTCATACTGATTCGCAGTATGTCCGCCAAGGGATCACTGAGTGG
ATGTCCGGTTGGGTACGGCGTGGTTGGAAAACTGCCGCCGGTGACCCGGTCAAGAATCGTGATCTATGGGAGCGGTTGTG
TGCTGCTACGCAGCGGCACATGATCGAATGGTGTTGGGTCAAGGCTCACAATGGTGATTCGGATAATGAACGGGTTGATG
TATTGGCCCGCGGCCAAGCGATGGCGCAGCGTAGTACGGTAGCAAGTCGATGA

Upstream 100 bases:

>100_bases
CTGCTTGAAGCTGATAAGCGCATGGTTGTACCGACTGGTTTGATTCCAGCCCGAGTAGGCTTGCGGCGTCCGTTGTTGGC
TTTCTGAACCTTTGGAATCT

Downstream 100 bases:

>100_bases
GGAATAAGAAGCGTCAGATTGTTCTTGATACTGAGACCACTGGCCTGGAATGGAGCAAGGGGAACCGCATTGTGGAGATC
GGTGCGGTCGAACTGCTTGA

Product: ribonuclease H

Products: NA

Alternate protein names: RNase HI [H]

Number of amino acids: Translated: 150; Mature: 150

Protein sequence:

>150_residues
MKLINAYTDGSCLGNPGPGGWAVLLRYKNNEKELVGGELDTTNNRMELMAAIMALERLSEPCQIKLHTDSQYVRQGITEW
MSGWVRRGWKTAAGDPVKNRDLWERLCAATQRHMIEWCWVKAHNGDSDNERVDVLARGQAMAQRSTVASR

Sequences:

>Translated_150_residues
MKLINAYTDGSCLGNPGPGGWAVLLRYKNNEKELVGGELDTTNNRMELMAAIMALERLSEPCQIKLHTDSQYVRQGITEW
MSGWVRRGWKTAAGDPVKNRDLWERLCAATQRHMIEWCWVKAHNGDSDNERVDVLARGQAMAQRSTVASR
>Mature_150_residues
MKLINAYTDGSCLGNPGPGGWAVLLRYKNNEKELVGGELDTTNNRMELMAAIMALERLSEPCQIKLHTDSQYVRQGITEW
MSGWVRRGWKTAAGDPVKNRDLWERLCAATQRHMIEWCWVKAHNGDSDNERVDVLARGQAMAQRSTVASR

Specific function: Endonuclease that specifically degrades the RNA of RNA- DNA hybrids [H]

COG id: COG0328

COG function: function code L; Ribonuclease HI

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 RNase H domain [H]

Homologues:

Organism=Homo sapiens, GI21359816, Length=143, Percent_Identity=35.6643356643357, Blast_Score=71, Evalue=4e-13,
Organism=Escherichia coli, GI1786408, Length=141, Percent_Identity=60.9929078014184, Blast_Score=182, Evalue=1e-47,
Organism=Caenorhabditis elegans, GI115532542, Length=146, Percent_Identity=33.5616438356164, Blast_Score=77, Evalue=5e-15,
Organism=Drosophila melanogaster, GI45552509, Length=148, Percent_Identity=37.8378378378378, Blast_Score=75, Evalue=2e-14,
Organism=Drosophila melanogaster, GI17137476, Length=148, Percent_Identity=37.8378378378378, Blast_Score=75, Evalue=2e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022892
- InterPro:   IPR012337
- InterPro:   IPR002156 [H]

Pfam domain/function: PF00075 RnaseH [H]

EC number: =3.1.26.4 [H]

Molecular weight: Translated: 16962; Mature: 16962

Theoretical pI: Translated: 8.33; Mature: 8.33

Prosite motif: PS50879 RNASE_H

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.7 %Cys     (Translated Protein)
4.7 %Met     (Translated Protein)
7.3 %Cys+Met (Translated Protein)
2.7 %Cys     (Mature Protein)
4.7 %Met     (Mature Protein)
7.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLINAYTDGSCLGNPGPGGWAVLLRYKNNEKELVGGELDTTNNRMELMAAIMALERLSE
CCEECCCCCCCCCCCCCCCCEEEEEEEECCCCEECCCCCCCCCCHHHHHHHHHHHHHCCC
PCQIKLHTDSQYVRQGITEWMSGWVRRGWKTAAGDPVKNRDLWERLCAATQRHMIEWCWV
CEEEEEECCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHEE
KAHNGDSDNERVDVLARGQAMAQRSTVASR
ECCCCCCCCCEEHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MKLINAYTDGSCLGNPGPGGWAVLLRYKNNEKELVGGELDTTNNRMELMAAIMALERLSE
CCEECCCCCCCCCCCCCCCCEEEEEEEECCCCEECCCCCCCCCCHHHHHHHHHHHHHCCC
PCQIKLHTDSQYVRQGITEWMSGWVRRGWKTAAGDPVKNRDLWERLCAATQRHMIEWCWV
CEEEEEECCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHEE
KAHNGDSDNERVDVLARGQAMAQRSTVASR
ECCCCCCCCCEEHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10910347 [H]